Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols

271 Pages·2016·7.8 MB·English

by  Alejandro Brun (eds.)

Methods in
Molecular Biology 1349
Alejandro Brun Editor
Vaccine
Technologies for
Veterinary Viral
Diseases
Methods and Protocols
M M B
ETHODS IN OLECULAR IOLOGY
Series Editor
John M. Walker
School of Life and Medical Sciences
University of Hertfordshire
Hat fi eld, Hertfordshire, AL10 9AB, UK
For further volumes:
http://www.springer.com/series/7651

Vaccine Technologies
for Veterinary Viral Diseases
Methods and Protocols
Edited by
Alejandro Brun
CISA-INIA, Valdeolmos, Madrid, Spain
Editor
Alejandro B run
CISA-INIA
Valdeolmos, Madrid , S pain
ISSN 1064-3745 ISSN 1940-6029 (electronic)
Methods in Molecular Biology
ISBN 978-1-4939-3007-4 ISBN 978-1-4939-3008-1 (eBook)
DOI 10.1007/978-1-4939-3008-1
Library of Congress Control Number: 2015949635
Springer New York Heidelberg Dordrecht London
© Springer Science+Business Media New York 2 016
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Prefa ce
A great amount of knowledge has been generated in recent years on veterinary vaccine
technologies, so presently many different antigen delivery systems are available for vaccine
research, in particular against viral diseases. This book was intended to review most popular
antigen production and delivery strategies that have been tested in veterinary species. Viral
vectors as well as genetic and protein subunit vaccines or large-scale protein production
systems are considered among the different chapters of the book. Our aim has been to
facilitate the access to real and well-established protocols to those beginning in this interest-
ing and laborious fi eld as well as to provide basic knowledge when attempting a novel vac-
cine design or platform. This book, in addition, will provide an updated view of most
options available when vaccine development is considered. In spite of the number of alter-
natives available for vaccine design, few of them often reach market and commercialization,
but, nonetheless, the data obtained along experimental trials contribute to the exploration
and understanding of the immune mechanisms and immune correlates relevant in protec-
tion among different animal species. Therefore this book also could be of help to those
interested in basic and applied immunology, since these technologies represent interesting
tools to induce or modulate different host immune mechanisms.
Valdeolmos, Madrid, Spain Alejandro Brun
v

Contents
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i x
1 Vaccines and Vaccination for Veterinary Viral Diseases:
A General Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Alejandro B run
2 U sing IC-Tagging Methodology for Production and Purification
of Epitope-Loaded Protein Microspheres for Vaccination . . . . . . . . . . . . . . . . 25
Natalia B arreiro-P iñeiro , R ebeca M enaya-Vargas , A lberto Brandariz-Núñez ,
Iria Otero-R omero , I rene L ostalé-Seijo , Javier Benavente ,
and José M . Martínez-Costas
3 P lant-Based Vaccine Antigen Production. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Hoang T rong P han and Udo C onrad
4 DNA Vaccines: Experiences in the Swine Model . . . . . . . . . . . . . . . . . . . . . . . 4 9
Francesc Accensi , F ernando R odríguez , and P aula L . M onteagudo
5 N ovel Adjuvants and Immunomodulators for Veterinary Vaccines. . . . . . . . . . 6 3
Peter M .H. Heegaard , Yongxiang F ang , and Gregers J ungersen
6 Polymerase Mechanism-Based Method of Viral Attenuation . . . . . . . . . . . . . . 8 3
Cheri A. Lee , Avery August , Jamie J . A rnold , and Craig E. C ameron
7 B acMam Platform for Vaccine Antigen Delivery . . . . . . . . . . . . . . . . . . . . . . . 1 05
Günther M. K eil , Reiko P ollin , Claudia M üller , Katrin Giesow ,
and H orst Schirrmeier
8 L aboratory-Scale Production of Replication-Deficient Adenovirus
Vectored Vaccines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Susan J . Morris , Alison V . T urner , N icola Green ,
and George M. Warimwe
9 G eneration of Recombinant Modified Vaccinia Virus Ankara
Encoding VP2, NS1, and VP7 Proteins of Bluetongue Virus. . . . . . . . . . . . . . 137
Alejandro Marín- López and Javier O rtego
10 G eneration of Recombinant Capripoxvirus Vectors
for Vaccines and Gene Knockout Function Studies . . . . . . . . . . . . . . . . . . . . . 1 51
Hani Boshra , Jingxin C ao , and Shawn B abiuk
11 Recombinant Swinepox Virus for Veterinary Vaccine Development. . . . . . . . . 1 63
Hong-Jie Fan and H ui-Xing L in
12 Generation and Selection of Orf Virus (ORFV) Recombinants . . . . . . . . . . . . 1 77
Hanns-Joachim R ziha , Jörg R ohde , and Ralf Amann
vii
viii Contents
13 Polycistronic Herpesvirus Amplicon Vectors
for Veterinary Vaccine Development . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 01
Anita F elicitas Meier , Andrea S ara Laimbacher ,
and Mathias Ackermann
14 C onstruction and Application of Newcastle Disease
Virus-B ased Vector Vaccines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 25
Paul J. Wichgers S chreur
15 Chimeric Pestivirus Experimental Vaccines . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 39
Ilona Reimann , S andra B lome , and Martin B eer
16 A nalysis of the Cellular Immune Responses to Vaccines. . . . . . . . . . . . . . . . . . 2 47
Nicholas S vitek , Evans L . N. Taracha , Rosemary Saya ,
Elias Awino , V ishvanath Nene , and Lucilla S teinaa
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 63
Contributors
FRANCESC A CCENSI • Centre de Recerca en Sanitat Animal (CReSA) , B ellaterra,
Barcelona, S pain ; D epartament de Sanitat i d’Anatomia Animals, U niversitat
Autònoma de Barcelona (UAB) , B ellaterra, Barcelona, S pain
MATHIAS A CKERMANN • Vetsuisse Faculty, Institute of Virology , University of Zurich , Z urich,
Switzerland
RALF AMANN • Institute of Immunology, F riedrich-Loeffl er-Institute , G reifswald,
Island of Riems, G ermany; Department of Immunology, Interfaculty Institute of Cell
Biology, Eberhard Karls Universität, Tübingen, Germany
JAMIE J. A RNOLD • Department of Biochemistry and Molecular Biology, T he Pennsylvania
State University , U niversity Park, P A , U SA
AVERY A UGUST • Department of Microbiology and Immunology, College of Veterinary
Medicine, C ornell University , Ithaca, NY , U SA
ELIAS AWINO • Vaccine Biosciences, I nternational Livestock Research Institute , N airobi,
Kenya
SHAWN BABIUK • Canadian Food Inspection Agency , W innipeg, M B , C anada
NATALIA BARREIRO-PIÑEIRO • Department of Biochemistry and Molecular Biology, Centro
Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS) ,
University of Santiago de Compostela , S antiago de Compostela, S pain
MARTIN BEER • Institute of Diagnostic Virology, Friedrich-Loeffl er-Institut , G reifswald,
Insel Riems, G ermany
JAVIER BENAVENTE • Department of Biochemistry and Molecular Biology, Centro Singular
de Investigación en Química Biológica y Materiales Moleculares (CIQUS) ,
University of Santiago de Compostela , Santiago de Compostela, Spain
SANDRA B LOME • Institute of Diagnostic Virology, F riedrich-Loeffl er-Institut ,
Greifswald, Insel Riems, G ermany
HANI BOSHRA • Canadian Food Inspection Agency , W innipeg, M B , C anada
ALBERTO B RANDARIZ-NÚÑEZ • Department of Biochemistry and Molecular Biology,
Centro Singular de Investigación en Química Biológica y Materiales Moleculares
(CIQUS) , U niversity of Santiago de Compostela , S antiago de Compostela, S pain ;
Department of Biochemistry, Center for Biophysics and Computational Biology,
University of Illinois at Urbana-Champaign , U rbana, I L, USA
ALEJANDRO B RUN • Centro de Investigación en Sanidad Animal (CISA) , I nstituto
Nacional de Investigación y Tecnología Agraria y Almentaria (INIA) , V aldeolmos ,
Madrid, Spain
CRAIG E . CAMERON • Department of Biochemistry and Molecular Biology,
The Pennsylvania State University , University Park, P A , U SA
JINXING CAO • Public Health Agency of Canada , Winnipeg, M B, Canada
UDO CONRAD • Department of Molecular Genetics, L eibniz Institute of Plant Genetics
and Crop Plant Research (IPK) , Gatersleben, G ermany
ix