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569 Pages·2016·20.705 MB·English
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Methods in Cell Biology The Zebrafish: Genetics, Genomics, and Transcriptomics Volume 135 Series Editors Leslie Wilson DepartmentofMolecular,CellularandDevelopmentalBiology UniversityofCalifornia SantaBarbara,California Phong Tran University of Pennsylvania Philadelphia, USA & Institut Curie, Paris, France Methods in Cell Biology The Zebrafish: Genetics, Genomics, and Transcriptomics Volume 135 Edited by H. William Detrich, III Northeastern University Marine Science Center, Nahant,MA,United States Monte Westerfield University of Oregon, Eugene,OR,United States Leonard I. Zon Harvard University, Boston,MA,United States AMSTERDAM(cid:129)BOSTON(cid:129)HEIDELBERG(cid:129)LONDON NEWYORK(cid:129)OXFORD(cid:129)PARIS(cid:129)SANDIEGO SANFRANCISCO(cid:129)SINGAPORE(cid:129)SYDNEY(cid:129)TOKYO AcademicPressisanimprintofElsevier AcademicPressisanimprintofElsevier 50HampshireStreet,5thFloor,Cambridge,MA02139,USA 525BStreet,Suite1800,SanDiego,CA92101-4495,USA 125LondonWall,LondonEC2Y5AS,UK TheBoulevard,LangfordLane,Kidlington,OxfordOX51GB,UK Fourthedition2016 Copyright©2016,2011,2004,1999ElsevierInc.Allrightsreserved. Nopartofthispublicationmaybereproducedortransmittedinanyformorbyanymeans, electronicormechanical,includingphotocopying,recording,oranyinformationstorage andretrievalsystem,withoutpermissioninwritingfromthepublisher.Detailsonhowto seekpermission,furtherinformationaboutthePublisher’spermissionspoliciesandour arrangementswithorganizationssuchastheCopyrightClearanceCenterandthe CopyrightLicensingAgency,canbefoundatourwebsite:www.elsevier.com/permissions. Thisbookandtheindividualcontributionscontainedinitareprotectedundercopyrightby thePublisher(otherthanasmaybenotedherein). Notices Knowledgeandbestpracticeinthisfieldareconstantlychanging.Asnewresearchand experiencebroadenourunderstanding,changesinresearchmethods,professional practices,ormedicaltreatmentmaybecomenecessary. Practitionersandresearchersmustalwaysrelyontheirownexperienceandknowledgein evaluatingandusinganyinformation,methods,compounds,orexperimentsdescribed herein.Inusingsuchinformationormethodstheyshouldbemindfuloftheirownsafety andthesafetyofothers,includingpartiesforwhomtheyhaveaprofessionalresponsibility. Tothefullestextentofthelaw,neitherthePublishernortheauthors,contributors,or editors,assumeanyliabilityforanyinjuryand/ordamagetopersonsorpropertyasa matterofproductsliability,negligenceorotherwise,orfromanyuseoroperationofany methods,products,instructions,orideascontainedinthematerialherein. ISBN:978-0-12-803474-3 ISSN:0091-679X ForinformationonallAcademicPresspublications visitourwebsiteathttps://www.elsevier.com Publisher:ZoeKruze AcquisitionEditor:ZoeKruze EditorialProjectManager:SarahLay ProductionProjectManager:MalathiSamayan Designer:VictoriaPearson TypesetbyTNQBooksandJournals Len,Monte,andIdedicatethe4thEditionofMethodsinCellBiology: The Zebrafish to the postdoctoral fellows and graduate students who conducted the genetic screens that established the zebrafish as a preeminent vertebrate model system for analysis of development. Contributors J. Ablain Howard Hughes Medical Institute and Harvard Medical School, Boston, MA, United States K. Asakawa SOKENDAI (TheGraduateUniversity forAdvanced Studies),Mishima, Shizuoka,Japan H. Ata Mayo Clinic, Rochester,MN, UnitedStates J. Bakkers HubrechtInstituteand University Medical Centre Utrecht, Utrecht,The Netherlands J. Bessa IBMC-InstitutodeBiologiaMoleculareCelular,Porto,Portugal;Universidadedo Porto,Porto, Portugal Y.M. Bradford University ofOregon, Eugene,OR, UnitedStates B.R. Cairns University ofUtah School ofMedicine,SaltLake City, UT,United States W.Chen VanderbiltUniversity School ofMedicine, Nashville, TN, UnitedStates J. Cibelli Michigan State University,East Lansing, MI,United States; BIONAND, Andalucı´a,Spain K.J. Clark Mayo Clinic, Rochester,MN, UnitedStates P. Coucke Ghent University,Ghent,Belgium V.T.Cunliffe University ofSheffield, Sheffield, United Kingdom F.DelBene PSL ResearchUniversity,Paris, France xvii xviii Contributors A.De Paepe Ghent University,Ghent, Belgium F. DeSantis PSL Research University,Paris, France V.Di Donato PSL Research University,Paris, France A.Eagle University ofOregon,Eugene,OR, United States S.C. Ekker Mayo Clinic,Rochester, MN, United States T.Erickson Oregon Health& Science University,Portland, OR, United States T.Evans WeillCornellMedical College, New York, NY,UnitedStates D.Fashena University ofOregon,Eugene,OR, United States A.Felker University ofZu¨rich,Zu¨rich, Switzerland A.Ferna´ndez-Min˜a´n Centro Andaluz de Biologı´a delDesarrollo (CABD), ConsejoSuperior de Investigaciones Cientı´ficas/Universidad Pablo de Olavide/Junta de Andaluc´ıa, Sevilla, Spain K.Frazer University ofOregon,Eugene,OR, United States A.Ghosha Carnegie Institution for Science,Baltimore, MD, United States M.G. Goll MemorialSloan Kettering Cancer Center,New York, NY,United States J.L. Go´mez-Skarmeta Centro Andaluz de Biologı´a delDesarrollo (CABD), ConsejoSuperior de Investigaciones Cientı´ficas/Universidad Pablo de Olavide/Junta de Andaluc´ıa, Sevilla, Spain aCurrentaddress:TataInstituteofFundamentalResearch,Mumbai,India Contributors xix D.J. Grunwald University ofUtah, SaltLake City, UT,United States M.E. Halpern Carnegie Institution for Science,Baltimore, MD, UnitedStates J.K. Heath Walterand Eliza Hall InstituteofMedical Research,Parkville, VIC, Australia K. Hoshijima University ofUtah, SaltLake City, UT,United States D.G. Howe University ofOregon, Eugene,OR, UnitedStates H. Huang University ofCalifornia LosAngeles, LosAngeles, CA, United States J.P.Junker HubrechtInstituteand University Medical Centre Utrecht, Utrecht,The Netherlands M.J. Jurynec University ofUtah, SaltLake City, UT,United States P. Kalita University ofOregon, Eugene,OR, UnitedStates K. Kawakami SOKENDAI(TheGraduateUniversityforAdvancedStudies),Mishima,Shizuoka, Japan M.C. Keightley Australian Regenerative Medicine Institute,Clayton, VIC, Australia; Monash University,Clayton,VIC, Australia F.Kruse HubrechtInstituteand University Medical Centre Utrecht, Utrecht, The Netherlands C. Lawrence Boston Children’s Hospital, Boston, MA, UnitedStates S. Lefever Ghent University,Ghent,Belgium C. Li Memorial Sloan KetteringCancerCenter,New York, NY,UnitedStates; Weill CornellGraduateSchool ofMedical Sciences, New York, NY,United States xx Contributors G.J.Lieschke Australian RegenerativeMedicine Institute,Clayton,VIC, Australia; Monash University,Clayton, VIC, Australia S.Lin University ofCalifornia Los Angeles,Los Angeles,CA, United States C.G. Love Walterand ElizaHall Institute ofMedical Research, Parkville,VIC, Australia; University ofMelbourne, Parkville, VIC, Australia L.A. Maddison Vanderbilt University School ofMedicine, Nashville,TN, United States P.Mani University ofOregon,Eugene,OR, United States S.Markmiller University ofCalifornia San Diego, La Jolla, CA,UnitedStates R.Martin University ofOregon,Eugene,OR, United States S.Masuda Tokyo Institute ofTechnology,Yokohama,Japan A.C. Miller University ofOregon,Eugene,OR, United States C.B. Moens Fred Hutchinson Cancer Research Center, Seattle,WA,United States C.Mosimann University ofZu¨rich,Zu¨rich, Switzerland S.T.Moxon University ofOregon,Eugene,OR, United States M.C. Mullins University ofPennsylvania Perelman School ofMedicine,Philadelphia,PA, United States P.J.Murphy University ofUtah School ofMedicine, Salt Lake City,UT, United States K.N. Murray University ofOregon,Eugene,OR, United States Contributors xxi A. Muto SOKENDAI (TheGraduateUniversity forAdvanced Studies),Mishima, Shizuoka,Japan T. Nicolson Oregon Health & Science University, Portland, OR, UnitedStates C.J. Ott Dana Farber CancerInstitute,Harvard Stem Cell Institute,Boston, MA, United States H. Paddock University ofOregon, Eugene,OR, UnitedStates F.Pelegri University ofWisconsineMadison, Madison, WI,United States C. Pich University ofOregon, Eugene,OR, UnitedStates S. Prukudom Kasetsart University, Bangkok, Thailand;Center ofExcellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, Thailand S. Ramachandran University ofOregon, Eugene,OR, UnitedStates J.E.J.Rasko Centenary Institute,Camperdown, NSW, Australia; University ofSydney, Sydney,NSW,Australia; Royal Prince Alfred Hospital, Newtown,NSW, Australia M.P. Rossmann Harvard University,Harvard,Cambridge, MA, UnitedStates L. Ruzicka University ofOregon, Eugene,OR, UnitedStates K. Schaper University ofOregon, Eugene,OR, UnitedStates A.N. Shah Fred Hutchinson CancerResearch Center,Seattle,WA,UnitedStates X. Shao University ofOregon, Eugene,OR, UnitedStates A. Singer University ofOregon, Eugene,OR, UnitedStates

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