The Use of Resealed Erythrocytes as Carriers and Bioreactors

341 Pages·1992·10.495 MB·English

by  Joseph F. Hoffman (auth.), Mauro Magnani, John R. DeLoach (eds.)

THE USE OF RESEALED
ERYTHROCYTES AS
CARRIERS AND
BIOREACTORS
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
Editorial Board:
NATHAN BACK, State University of New York at Buffalo
IRUN R. COHEN, The Weizmann Institute of Science
DAVID KRITCHEVSKY, Wistar Institute
ABEL LAJTHA, N.S. Kline Institute for Psychiatric Research
RODOLFO PAOLETTI, University of Milan
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THE USE OF RESEALED
ERYTHROCYTES AS
CARRIERS AND
BIOREACTORS
Edited hy
Mauro Magnani
University of Urbino
Urbino, Italy
and
lohn R. DeLoach
United States Department of Agriculture
Agricultural Research Service
Food Animal Protection Research Laboratory
College Station, Texas
SPRINGER SCIENCE+BUSINESS MEDIA, LLC
Llbrary of Congress Cataloglng-ln-Publlcatlon Data
The Use of resealed erythrocytes as carriers and bioreactors / edited
by Mauro Magnani and John R. DeLoach.
p. cm. -- (Advances in experimental medicine and biology ; v.
326)
"Proceedings of the Fourth International Meeting of the
International Society for the Use of Resealed Erythrocytes as
Carriers and Bioreactors, held September 5-7, 1991, in Urbino,
Italy. "--T.p. verso.
Includes bibI iographical references and index.
ISBN 978-1-4613-6321-7 ISBN 978-1-4615-3030-5 (eBook)
001 10.1007/978-1-4615-3030-5
1. Erythrocytes--Biotechnology--Congresses. 2. Bioreactors-
-Congresses. 3. Drugs--Vehicles--Congresses. I. Magnani, Mauro.
11. DeLoach, J. R. (John R.) III. International Society for the Use
of Resealed Erythrocytes as Carriers and Bioreactors. International
Meeting (4th 1991 Urbino, Italy) IV. Series.
[DNLM: 1. Drug Carriers--congresses. 2. Erythrocytes--congresses.
Wl AD559 v. 326 / WH 150 UB4 19911
TP248.27.A53U84 1992
615' . 19--dc20
DNLM/DLC
for Library of Congress 92-48773
CIP
Proceedings of the Fourth International Meeting of the International
Society for The Use of Resealed Erythrocytes as Carriers and Bioreactors,
held September 5-7, 1991, in Urbino, Italy
© 1992 by Springer Science+Business Media New York
Originally published by Plenum Press, New York in 1992
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise, without written permission from the Publisher
This book is dedicated to our families
PREFACE
Until recently the only biomedical use of erythrocytes was in transfusion medicine
to restore a normal oxygen delivery. The development of a technology that permits one to
open and reseal erythrocytes has dramatically changed this perspective. Currently, a
number of teams have shown that engineered erythrocytes can behave as circulating
bioreactors for the degradation of toxic metabolites or the inactivation of xenobiotics, as
drug delivery systems, as carriers of antigens of vaccinal interest, and in many others
biomedical applications. The technology of opening and resealing the erythrocytes has also
been used successfully to investigate several basic aspects of erythrocyte metabolism,
survival, pathology, etc. Thus, researchers in this field have an extraordinary opportunity to
specifically modify the erythrocytes by the introduction of enzymes that generate new
metabolic abilities, antibodies that inactivate single metabolic steps, or metabolites that can
influence oxygen delivery and/or other cell properties. Furthermore, the pharmacokinetics
of any drug can be potentially manipulated by using the erythrocytes as a delivery system.
This book, The Use of Resealed Erythrocytes, is based on the fourth meeting of the
"International Society for the Use of Resealed Erythrocytes as Carriers and Bioreactors"
(I.S.U.R.E.), held in Urbino, Italy, in 1991, and examines the most recent applications and
developments of this technology. Since the first biennial meeting of this Society, in 1984,
new applications, methodological improvements, and advancements in basic cell biology
have been characteristic of these meetings and this volume in particular reports new
technical approaches and new applications. Therefore, this book can certainly provide an
overview of the most recent advances in the field and, we hope, can also be inspiring for
those researchers that are not yet familiar with this approach.
vii
CONTENTS
On Red Blood Cells, Hemolysis and Resealed Ghosts ........................................................ 1
J.F. Hoffman
METHODS AND PROCEDURES FOR PREPARING RESEALED ERYTHROCYTES
IHP Entrapment into Human Erythrocytes: Comparison Between
Hypotonic Dialysis and DMSO Osmotic Pulse ....................................................... 19
A. Mosca, R. Paleari, V. Russo, E. Rosti, R. Nano, A. Boicelli,
S. Villla, and A. Zanella
Density Gradient Separation of Inositol Hexaphosphate Loaded
Red Blood Cells in Various Preparation Conditions ............................................... 27
G. Bourget, L. Boucher, and C. Ropars
Quantitative IHP Determination by 31P-NMR: Proposal for a
Standardized Protocol ............................................................................................ 35
R. Nano, A. Mosca, R. Paleari, and A. Boicelli
Determination of Inositol Hexaphosphate (IHP) in Human IHP-Loaded
Red Blood Cells by a Simple High Performance Liquid
Chromatography Method ...................................................................................... 41
S. Villa, F. Rossi, P.A. Biondi, V. Russo, T. Crimella, G. Fiorelli, and
A. Zanella
Determination of Purity of Commercially Available Inositol
Hexaphosphate (Phytates) and Preparation of a Reference
Material ................................................................................................................ 51
T. Crimella, S. Villa, F. Rossi, G. Fiorelli, and A. Zanella
Standardization of an Encapsulation System: A Method to
Remove Fragile Cells ........................................................................................... 55
L. Chiarantini, and J.R. DeLoach
The Dialysis Encapsulation Process: The Role of Endocytosis ...................................... 63
K. Andrews, R.E. Droleskey, L. Chiarantini, and J.R. DeLoach
Use of Fluorescent Probes for Describing the Process of
Encapsulation by Hypotonic Dialysis ................................................................... 73
R.E. Droleskey, K. Andrews, L. Chiarantini, and 1.R. DeLoach
ix
Properties of Hypotonized, Crosslinked and Crosslinked-
Permeabilized Rat Erythrocytes As Potential Carrier Systems ................................ 81
J. Luque, M.l. Garin, S. Sanz, P. Ropero and M. Pinilla
Membrane Properties of Senescent and Carrier Human Erythrocytes ............................. 91
M.A. Castellana, M.R. De Renzis, G. Piccinini, G. Minetti,
C. Seppi, C. Balduini, and A. Brovelli
Studies with Biotinylated RBC: (1) Use of Flow Cytometry to Determine
Posttransfusion Survival and (2) Isolation Using Streptavidin
Conjugated Magnetic Beads ................................................................................. 101
Y. Russo, R. Barker-Gear, R. Gates, and R. Franco
RESEALED ERYTHROCYTES AS A TOOL FOR BASIC STUDIES
Resealing of Protein Tyrosine Kinase Substrates Into Human
Erythrocytes by Rapid Freezing and Thawing in Liquid
Nitrogen .............................................................................................................. 111
M. Harrison, C. Isaacson, P. Rathinavelu, and P.S. Low
The Metabolic Role of Glucose 1,6-P in Human Erythrocytes
2
Studied by Encapsulation Procedures .................................................................. 119
A. Accorsi, E. Piatti, M.P. Piacentini, and A. Fazi
The Application of Newer Concepts of Oxygen Transport to the
Evaluation of the Potential Utility of Reduced Hb-0 Affinity ............................ 127
2
M. Weiner
Single Cell Analysis of Factors Increasing the Survival of
Resealed Erythrocytes in the Circulation of Mice ............................................... 133
R.A. Schlegel, K. Lumley-Sapanski, and P. Williamson
Entrapment of Purified a-Hemoglobin Chains in Normal
Erythrocytes as a Model for Human pThalassemia ............................................. 139
M.D. Scott
AT P Monitoring in Human Red Blood Cells with Luciferase
Introduced Intracellularly ................................................................................... 149
Y.M. Yitvitsky, F.A. Ataullakhanov, and E.I. Sinauridze
RESEALED ERYTHROCYTES AS CELLULAR BIOREACTORS
Antagonism of the Lethal Effects of Cyanide with Resealed
Erythrocytes Containing Rhodanese and Thiosulfate ......................................... 159
J.L. Way, E.P. Cannon, P. Leung, A. Hawkins-Zitzer,
Luqi Pei, and I. Petrikovics
Acetaldehyde Oxidation by Aldehyde Dehydrogenase
Loaded Erythrocytes .......................................................................................... 165
P. Ninfali, L. Rossi, L. Baronciani, C. Ropars, and M. Magnani
x
Immunological Response to L-Asparaginase Loaded into
Red Blood Cells ................................................................................................... 175
R. Kravtzoff, I. Desbois, C. Doinel, P. Colombat, J.P. Lamagnere,
M. Chassaigne, and C. Ropars
Normalization of Hyperglycemia in Diabetic Mice by Enzyme-
Loaded Erythrocytes ............................................................................................ 183
L. Rossi, M. Bianchi, A. Fraternale, and M. Magnani
Comparison of Uricase-Bound and Uricase-Loaded Erythrocytes
as Bioreactors for Uric Acid Degradation ............................................................. 189
M. Magnani, U. Mancini, M. Bianchi, and A. Fazi
Xenobiotic Detoxification by GSH-Loaded Erythrocytes .............................................. 195
A. Fazi, U. Mancini, E. Piatti, A. Accorsi, and M. Magnani
A New Chemotherapeutic Agent: L-Asparaginase Entrapped
in Red Blood Cells ............................................................................................... 203
E.1. Sinauridze, V.M. Vitvitsky, A.V. Pichugin,
A.M. Zhabotinsky, and F.1. Ataullakhanov
RESEALED ERYTHROCYTES AS ADVANCED DRUG
DELIVER Y SYSTEMS
Rubomycin Loaded Erythrocytes in the Treatment of Mouse
Tumor P388 .......................................................................................................... 209
F.I. Ataullakhanov, V.M. Vitvitsky, V.L. Kovaleva, and
B. Mironova
Erythrocytes as Carriers of New Anti-Opioid Prodrugs:
In Vitro Studies .................................................................................................... 215
S. Noel-Hocquet, S. Jabbouri, S. Lazar, J.e. Maunier,
G. Guillaumet, and e. Ropars
Interactions of Carboplatin with Human Erythrocytes and
Murine Erythroleukemic Cells ............................................................................. 223
M. Tonetti, A. Gasparini, M. Giovine, U. Benatti, and
A. De Flora
Modulation of Thiopurine Metabolism in Native Human Erythrocytes ......................... 233
C. Salerno, A. Lucano, and e. Crifo
Red Blood Cells as Advanced Drug Delivery Systems for
Antiviral Nucleoside Analogues ........................................................................... 239
M. Magnani, L. Rossi, A. Casabianca, A. Fraternale,
G. Schiavano, G. Brandi, F. Mannello, and
G. Piedimonte
An Optimal Control Problem for the Administration of a
Drug by Using Red Blood Cells as Bioreactors .................................................... 247
E. Beretta, GJ. Bischi, and F. Solimano
xi
SITE SPECIFIC TARGETING OF RESEALED ERYTHROCYTES
Targeting of Erythrocytes to Cytotoxic T -Cells ............................................................. 257
L. Chiarantini, R. Droleskey, M. Magnani, H. Kirch, and
J.R. DeLoach
Introduction to In Vivo Targeting .................................................................................. 269
L. Chiarantini, R.E. Droleskey, and J.R. DeLoach
Erythrocytes as Carriers of Ricin A Chain: Effects on the
Erythrophagocytic Cells ....................................................................................... 279
N. Chestier, R. Kravtzoff, S. Canepa, M. Chassaigne, and
C. Ropars
In Vitro Targeting of Doxorubicin Loaded Canine Erythrocytes
to Cytotoxic T-Lymphocytes (CTLL) .................................................................. 291
A. Gasparini, L. Chiarantini, H. Kirch, and lR. DeLoach
Pharmacokinetics of Doxorubicin Loaded and Glutaraldehyde
Treated Erythrocytes in Healthy and Lymphoma Bearing
Dogs .................................................................................................................... 299
A. Gasparini, M. Tonetti, B. Astroff, L. Rowe, W. Satterfield,
R. Schmidt, and 1.R. DeLoach
HUMAN AND VETERINARY STUDIES USING
RESEALED ERYTHROCYTES
Use of Glutaraldehyde Treated Autologous Human
Erythrocytes for Hepatic Targeting of Doxorubicin .............................................. 307
M. Tonetti, E. Zocchi, L. Guida, C. Polvani, U. Benatti,
P. Biassoni, F. Romei, A. Guglielmi, C. Aschele,
A. Sobrero, and A. De Flora
Clinical Evaluation of Glutaraldehyde-Treated Canine
Erythrocytes in Normal Dogs ............................................................................... 319
W.C. Satterfield, C.M. Matherne, M.S. Clarke, and
1.R. DeLoach
Sickle Cells Modified by an Osmotic Pulse in the Presence
of Inositol Hexaphosphate have Decreased Intracellular
Hemoglobin Concentration and Decreased In Vitro
Sickling without Prolonged In Vivo Survival ....................................................... 325
R. Franco, R. Barker-Gear, E. Silberstein, G. Mayfield,
M. Weiner, 1. Palascak, and R. Green
A Baboon Model to Test Physiological and Adverse Effects
of Human Red Cells Loaded with Inositol
Hexaphosphate (IHP) ........................................................................................... 333
P. Di Mauro, M. Langer, P. Prato, B. Gridelli, S. Gatti,
G. Fiorelli, S. Villa, F. Rossi, and A. Zanella
xii