Techniques in Genetic Engineering PDF
Preview Techniques in Genetic Engineering
LIFE SCIENCES Kurnaz Techniques in Techniques in GENETIC ENGINEERING GENE TIC T e c Although designed for undergraduates with an interest in molecular biology, h ENGINEERING n biotechnology, and bioengineering, this book—Techniques in Genetic i q Engineering—IS NOT a laboratory manual; nor is it a textbook on molecular u biology or biochemistry. There is some basic information in the appendices about e s core concepts such as DNA, RNA, protein, genes, and genomes; however, in i general it is assumed that the reader has a background on these key issues. n G Techniques in Genetic Engineering briefly introduces some common genetic E engineering techniques and focuses on how to approach different real-life problems using a combination of these key issues. Although not an exhaustive N review of these techniques, basic information includes core concepts such as E DNA, RNA, protein, genes, and genomes. It is assumed that the reader has a T background on these key issues. The book provides sufficient background and I future perspectives for the readers to develop their own experimental strategies C and innovations. E This easy-to-follow book presents not only the theoretical background of N molecular techniques, but also provides case study examples, with some G sample solutions. The book covers basic molecular cloning procedures; genetic modification of cells, including stem cells; as well as multicellular organisms, I N using problem-based case study examples. E E R I N G K24275 Işıl Aksan Kurnaz 6000 Broken Sound Parkway, NW Suite 300, Boca Raton, FL 33487 711 Third Avenue New York, NY 10017 an informa business 2 Park Square, Milton Park www.crcpress.com Abingdon, Oxon OX14 4RN, UK www.crcpress.com Techniques in GENETIC ENGINEERING Techniques in GENETIC ENGINEERING Işıl Aksan Kurnaz Boca Raton London New York CRC Press is an imprint of the Taylor & Francis Group, an informa business CRC Press Taylor & Francis Group 6000 Broken Sound Parkway NW, Suite 300 Boca Raton, FL 33487-2742 © 2015 by Taylor & Francis Group, LLC CRC Press is an imprint of Taylor & Francis Group, an Informa business No claim to original U.S. Government works Version Date: 20150409 International Standard Book Number-13: 978-1-4822-6090-8 (eBook - PDF) This book contains information obtained from authentic and highly regarded sources. Reasonable efforts have been made to publish reliable data and information, but the author and publisher cannot assume responsibility for the validity of all materials or the consequences of their use. The authors and publishers have attempted to trace the copyright holders of all material reproduced in this publication and apologize to copyright holders if permission to publish in this form has not been obtained. If any copyright material has not been acknowledged please write and let us know so we may rectify in any future reprint. Except as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced, transmitted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter invented, including photocopying, microfilming, and recording, or in any information stor- age or retrieval system, without written permission from the publishers. For permission to photocopy or use material electronically from this work, please access www.copy- right.com (http://www.copyright.com/) or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that pro- vides licenses and registration for a variety of users. For organizations that have been granted a photo- copy license by the CCC, a separate system of payment has been arranged. Trademark Notice: Product or corporate names may be trademarks or registered trademarks, and are used only for identification and explanation without intent to infringe. Visit the Taylor & Francis Web site at http://www.taylorandfrancis.com and the CRC Press Web site at http://www.crcpress.com To my husband and partner in life, my two adorable kids, my parents and my family, my devoted assistants, and all my students … We must not forget that when radium was discov- ered no one knew that it would prove useful in hospitals. The work was done of pure science. And this is a proof that scientific work must not be con- sidered from the point of view of the direct useful- ness of it. It must be done for itself, for the beauty of science, and then there is always the chance that a scientific discovery may become like the radium a benefit for humanity. Marie Curie (1867–1934) Lecture at Vassar College, May 14, 1921
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