Supramolecular Structure and Function 10

159 Pages·2011·4.152 MB·English

by  Sergey Ivanchenko, Don C. Lamb (auth.), Jasminka Brnjas-Kraljević, Greta Pifat-Mrzljak (eds.)

Supramolecular Structure and Function 10

·
Jasminka Brnjas-Kraljevic´ Greta Pifat-Mrzljak
Editors
Supramolecular Structure
and Function 10
123
Editors
JasminkaBrnjas-Kraljevic´ GretaPifat-Mrzljak†
SchoolofMedicine Rud–erBoškovic´Institute
UniversityofZagreb 10000Zagreb
10000Zagreb Croatia
Croatia [e-mail]
[e-mail]
ISBN978-94-007-0892-1 e-ISBN978-94-007-0893-8
DOI10.1007/978-94-007-0893-8
SpringerDordrechtHeidelbergLondonNewYork
LibraryofCongressControlNumber:2011924004
©SpringerScience+BusinessMediaB.V.2011
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Preface
In the fifties of the last century the definition of biophysics arose much dispute
among the scientists who were traditional physicists, chemists and biologists by
training.Asaninterdisciplinaryscience,biophysicssharessignificantoverlapwith
biochemistry, bioengineering and systems biology, but on the other hand offers a
rational language for discussion about a common subject to scientists of differ-
entscientificdisciplines.Biophysicshasgraduallyerasedthefrontiersinscientific
researchbybringingtogetherscientistsfromdifferentfieldsofresearch.Nowadays,
it has been widely accepted that the search for new knowledge depends not only
on new methods and concepts but also on the interaction within different fields of
research.Promotinganinteractionbetweendifferentdisciplinesinnaturalsciences
andenablingyoungscientiststobeinvolvedinitisthegeneralphilosophybehind
the Biophysical Summer Schools organized by the Rudjer Boškovic´ Institute,
Zagreb,CroatiaandtheCroatianBiophysicalSocietyevery3years,since1981.
The International Summer Schools on Biophysics have a broad scope devoted
to the structure-function relationship of biological macromolecules and to mayor
biophysicaltechniques.Theyareinternationallyrecognizedandsuccessfullyestab-
lished under the title “Supramolecular Structure and Function” and are included
into the curricula of doctoral studies at distinguished European universities. The
intention has remained the same through all the ten Schools – to organize courses
which provide advanced training at doctoral or postdoctoral level in biosciences.
The Schools have gained reputation for running Discussion Clubs as extra curric-
ular activities, where students would invite their peers to gather around lecturers
anddiscussvarioustopicsofspecificinterest.Theenthusiasmofthesediscussions
is always equally shared by students and lecturers. The contributions presented at
the Summer School by prominent lecturers illustrate the principles, concepts and
methodsofbiophysicscoupledwithmolecularbiologyapproaches.Giventhecon-
siderablediversityoftopicsitcovers,webelievethatthebookwillbeofinterestto
scientistsinvolvedindifferentdisciplines,asitwastotheaudienceattheSummer
School.
The tenth Summer School, as Master Classes of UNESCO, was supported
by UNESCO and could be considered as a part of the mosaic forming the
EuropeanResearchArea(ERA)andtheEuropeanHigherEducationArea(EHEA).
v
vi Preface
The organizers of the International Summer School on Biophysics hope that the
publicationofthisvolumeanditsdistributionwithinthescientificcommunitywill
serve towards the objectives of expanding, sharing and providing easy access to
scientificknowledgeinthefieldofbiophysics.
The support to the School by IUPAB and EBSA reflects the international and
Europeaninteresttobringtogetherscientistsofdifferentprofilesfromalloverthe
world. The national financial supporters were the Ministry of Science, Education
andSportoftheRepublicofCroatia,theCroatianAcademyofSciencesandArts,
TheAdrisFoundationandTheNationalFoundationforScience,HigherEducation
and Technological Development of the Republic of Croatia whose substantial
supportenabledtheparticipationofyoungscientistsfromCroatia.
This volume will inform the broader scientific community on the profile of the
Summer School and new biophysical achievements, but the most valuable out-
comeofthetenthSchoolistheexchangeofknowledgeandfriendshipsestablished
between lecturers and participants in the pleasant atmosphere of the Crveni otok
nearRovinj,Croatia.
Zagreb,Croatia GretaPifat-Mrzljak
DirectoroftheSchools
ThespiritusmovensofalltenSchoolsduringtheperiodof30yearswasprofessor
GretaPifat-Mrzljak.Unofortunately,shepassedawayDecember11,2009.Tillthe
lastshewasinvolvedinorganizationandpreparationoftheSchoolandthisproceed-
ing.Thetradition,sheestablished,wastopresenttheSchoolwithbookofselected
lecturesheldonSchoolbydistinguishedlectures.Theintentionwastoacquaintthe
brooder scientific communion with the hot subjects in biophysical research. The
resultof30yearsdevotementistheserialoftenbooks“SupramolecularStructure
andFunction”asanicehistoryofbiophysicalresearchdevelopmentandagreathelp
ineducationofyoungscientistinthatfield.DetailedinformationaboutSchoolsand
bookscanbefoundonwebsiteoftheSchool
http://www.irb.hr/events/confpages/biophysics/
For the outstanding record of accomplishments and leadership of the trien-
nial international summer schools and textbooks on Supramolecular Structure and
Function prof. Greta Pifat-Mrzljak was presented the 2010 Emily M. Gray Award
byTheBiophysicalSociety.
Zagreb,Croatia JasminkaBrnjas-Kraljevic´
onbehalfofOrganizingCommittee
Contents
FluorescenceCorrelationSpectroscopy:PrinciplesandDevelopments . 1
SergeyIvanchenkoandDonC.Lamb
Time-Resolved FT-IR Spectroscopy for the Elucidation of
ProteinFunction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
MichaelSchleeger,IonelaRadu,andJoachimHeberle
Recombinant Membrane ProteinProduction: Past,Present
andFuture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
RaviK.R.Marreddy,EricR.Geertsma,andBertPoolman
ColdDenaturationandProteinStability . . . . . . . . . . . . . . . . . . 75
PieroAndreaTemussi
PolyglutamineDiseasesandNeurodegeneration:TheExample
ofAtaxin-1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
CesiradeChiaraandAnnalisaPastore
PhasePlateElectronMicroscopy . . . . . . . . . . . . . . . . . . . . . . 101
KuniakiNagayama
DerivingBiomedicalDiagnosticsfromSpectroscopicData . . . . . . . . 115
IanC.P.SmithandRayL.Somorjai
TheEmergenceandOzoneTreatmentStudiesofLivingCells . . . . . . 125
DavorPavuna,BožidarPavelic´,OgnjenPavicˇevic´,
DomagojPrebeg,andMarioZovak
ToxicityStudyofNanofibers . . . . . . . . . . . . . . . . . . . . . . . . 133
LenkeHorváth,ArnaudMagrez,BeatSchwaller,andLászlóForró
SubjectIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
vii

Contributors
CesiradeChiara NationalInstituteforMedicalResearch–MRC,NW71AA
London,UK,[e-mail]
LászlóForró LaboratoryofPhysicsofComplexMatter,EcolePolytechnique
FédéraledeLausanne,1015 Lausanne,Switzerland,Laszlo.forro@epfl.ch
EricR.Geertsma DepartmentofBiochemistry,GroningenBiomolecular
SciencesandBiotechnologyInstitute,NetherlandsProteomicsCentreandZernike
InstituteforAdvancedMaterials,UniversityofGroningen,Nijenborgh4,9747AG
Groningen,TheNetherlands,[e-mail]
JoachimHeberle ExperimentalMolecularBiophysics,DepartmentofPhysics,
FreeUniversityofBerlin,D-14195 Berlin,Germany,[e-mail]
LenkeHorváth DepartmentofMedicine,UnitofAnatomy,Universityof
Fribourg,1700 Fribourg,Switzerland;LaboratoryofPhysicsofComplexMatter,
EcolePolytechniqueFédéraledeLausanne,1015 Lausanne,Switzerland,
lenke.horvath@epfl.ch
SergeyIvanchenko DepartmentforChemistryandBiochemistry,Centerfor
Nanoscience(CeNS)andMunichCenterforIntegratedProteinScience(CiPSM),
Ludwig-Maximilians-UniversitätMünchen,81377 Munich,Germany,
[e-mail]
DonC.Lamb DepartmentforChemistry,CenterforNanoscience(CeNS)and
MunichCenterforIntegratedProteinScience(CiPSM),Ludwig-Maximilians-
UniversitätMünchen,81377 Munich,Germany;DepartmentofPhysics,
UniversityofIllinoisatUrbana-Champaign,Urbana,IL61801,USA,
[e-mail]
ArnaudMagrez LaboratoryofPhysicsofComplexMatter,EcolePolytechnique
FédéraledeLausanne,1015 Lausanne,Switzerland,arnaud.magrez@epfl.ch
RaviK.R.Marreddy DepartmentofBiochemistry,GroningenBiomolecular
SciencesandBiotechnologyInstitute,NetherlandsProteomicsCentreandZernike
ix
x Contributors
InstituteforAdvancedMaterials,UniversityofGroningen,Nijenborgh4,9747AG
Groningen,TheNetherlands,[e-mail]
KuniakiNagayama NationalInstituteforPhysiologicalSciences,OkazakiCity,
Aichi444-8787,Japan;TheGraduateUniversityforAdvancedStudies,Schoolof
PhysiologicalSciences,Hayamacho,Kanagawa240-0193,Japan,
[e-mail]
AnnalisaPastore NationalInstituteforMedicalResearch–MRC,NW71AA,
London,UK,[e-mail]
BožidarPavelic´ SchoolofDentalMedicine,UniversityofZagreb,Zagreb,
Croatia,[e-mail]
OgnjenPavicˇevic´ Biozondoo,Buzinskiprilaz1010000Zagreb,Croatia,
[e-mail]
DavorPavuna PhysicsSection,EPFL,CH-1015 Lausanne,Switzerland,
davor.pavuna@epfl.ch
BertPoolman DepartmentofBiochemistry,GroningenBiomolecularSciences
andBiotechnologyInstitute,NetherlandsProteomicsCentreandZernikeInstitute
forAdvancedMaterials,UniversityofGroningen,Nijenborgh4,9747AG
Groningen,TheNetherlands,[e-mail]
DomagojPrebeg Biozondoo,Buzinskiprilaz1010000Zagreb,Croatia,
[e-mail]
IonelaRadu ExperimentalMolecularBiophysics,DepartmentofPhysics,Free
UniversityofBerlin,D-14195 Berlin,Germany,[e-mail]
MichaelSchleeger ExperimentalMolecularBiophysics,DepartmentofPhysics,
FreeUniversityofBerlin,D-14195 Berlin,Germany,
[e-mail]
BeatSchwaller DepartmentofMedicine,UnitofAnatomy,Universityof
Fribourg,1700 Fribourg,Switzerland,[e-mail]
IanC.P.Smith InstituteforBiodiagnostics,NationalResearchCouncil
Winnipeg,Winnipeg,MB,CanadaR3B1Y6,[e-mail]
RayL.Somorjai InstituteforBiodiagnostics,NationalResearchCouncil
Winnipeg,Winnipeg,MB Canada,R3B1Y6,[e-mail]
PieroAndreaTemussi DipartimentodiChimica,Universita’diNapoliFederico
II,I-80126 Napoli,Italy;NationalInstituteforMedicalResearch–MRC,
NW71AA London,UK,[e-mail]
MarioZovak CroatiaSchoolofMedicine,UniversityofZagreb,Zagreb,Croatia,
[e-mail]