studies on dry root-rot disease of citrus: molecular characterization of potential native fungal ...

126 Pages·2016·1.77 MB·English

"STUDIES ON DRY ROOT-ROT DISEASE OF
CITRUS: MOLECULAR CHARACTERIZATION OF
POTENTIAL NATIVE FUNGAL ANTAGONIST AND
BIOLOGICAL CONTROL"

By
K. VISWANATH,
B.Sc., (Ag.)

THESIS SUBMITTED TO THE
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
IN PARTIAL FULFILMENT OF THE REQUIREMENTS
FOR THE AWARD OF THE DEGREE OF
MASTER OF SCIENCE IN AGRICULTURE
(PLANT PATHOLOGY)

DEPARTMENT OF PLANT PATHOLOGY
SRI VENKATESWARA AGRICULTURAL COLLEGE
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
HYDERABAD (A.P.)

SEPTEMBER, 2003
CERTIFICATE

This is to certify that the thesis entitled "STUDIES ON DRY ROOT-ROT
DISEASE OF CITRUS: MOLECULAR CHARACTERIZATION OF
POTENTIAL NATIVE FUNGAL ANTAGONIST AND BIOLOGICAL
CONTROL” submitted in partial fulfilment of the requirements for the
degree of 'Master of Science in Agriculture' of the Acharya N.G.Ranga
Agricultural University, Hyderabad, is a record of the bonafide research
work carried out by Mr. K. VISWANATH under our guidance and
supervision. The subject of the thesis has been approved by the Student's
Advisory Committee.

No part of the thesis has been submitted by the student for any other
degree or diploma. The published part has been fully acknowledged. All
assistance and help received during the course of the investigations have
been duly acknowledged by the author of the thesis.

(Dr. K. SEETHA RAMA REDDY)
Chairman of the Advisory Committee

Thesis approved by the Student Advisory Committee

Chairman (Dr. K. SEETHA RAMA REDDY) ______________________
Professor & Head
Department of Plant Pathology
S.V.Agricultural College
Tirupati - 517 502

Member (Dr. K. GOPAL) ______________________
Scientist (Plant Pathology)
A.I.C.R.P. on Tropical Fruits (Citrus)
S.V. Agricultural College Campus
Tirupati - 517 502

Member (Dr. N. VENUGOPALA RAO) ______________________
Professor & Head
Department of Entomology
S.V.Agricultural College
Tirupati - 517 502
CERTIFICATE

This is to certify that Mr. K. VISWANATH, has satisfactorily prosecuted
the course of research and that the thesis entitled “STUDIES ON DRY ROOT-
ROT DISEASE OF CITRUS: MOLECULAR CHARACTERIZATION OF
POTENTIAL NATIVE FUNGAL ANTAGONIST AND BIOLOGICAL
CONTROL” submitted is the result of original research work and is of
sufficiently high standard to warrant its presentation to the examination. I also
certify that the thesis or part thereof has not been previously submitted by her for
a degree of any University.

Date : (Dr. K. SEETHA RAMA REDDY)
Major Advisor
Place :
CONTENTS

Chapter Title Page No.

I INTRODUCTION

II REVIEW OF LITERATURE

III MATERIALS AND METHODS

IV RESULTS

V DISCUSSION

VI SUMMARY

LITERATURE CITED
DECLARATION

I, Mr. K. VISWANATH, hereby declare that the thesis entitled
“STUDIES ON DRY ROOT-ROT DISEASE OF CITRUS: MOLECULAR
CHARACTERIZATION OF POTENTIAL NATIVE FUNGAL
ANTAGONIST AND BIOLOGICAL CONTROL” submitted to Acharya N.G.
Ranga Agricultural University, Hyderabad for the degree of MASTER OF
SCIENCE IN AGRICULTURE is the result of original research work done by
me. I also declare that the materials contained in this thesis has not been
published earlier.

Date : (K. VISWANATH)
LIST OF TABLES

S Page
No. Title No.

Dry root – rot Incidence in Nalgonda, Ananthapur and
1
Prakasam Districts of Andhra Pradesh.

Number of soil samples collected and Trichoderma
2
species isolated from Nalgonda, Ananthapur and
Prakasam Districts of Andhra Pradesh.

Categorisation of Trichoderma isolates based on growth
3
rate

In vitro Screening of Trichoderma isolates against
4
Fusarium solani by dual culture.

Effect of Trichoderma on the growth of Fusarium solani
5
through production of volatile substances

Screening of bacterial isolates against Fusarium solani by
6
dual culture.

Morphological characters of 17 Trichoderma isolates
7

Effect of isolated Trichoderma, Pseudomonas spp. and
8.
organic amendments on incidence of dry root-rot and
plant growth in pot culture
LIST OF ILLUSTRATIONS

Fig. Page
No. Title No.

Incidence of dry root rot of sweet orange in Ananthapur,
1
Nalgonda and Prakasam districts of Andhra Pradesh

In vitro screening of Trichoderma isolates against
2
Fusarium solani by dual culture

Effect of Trichoderma on the growth of Fusarium solani
3
through production of volatile substances

Screening of bacterial isolates against Fusarium solani
4
by dual culture

Relative mobility (Rm) values of protein banding pattern
5
of Trichoderma isolates

Relative mobility (Rm) values of protein banding pattern
6
of Trichoderma isolates

Effect on the isolated Trichoderma, Pseudomonas spp.
7
and organic amendments incidence of dry root rot and

plant growth* in pot cultures
LIST OF SYMBOLS AND ABBREVIATIONS

% - Percentage
AICRP - All India Co-ordinated Research Project.
cm - centimeter
Fig. - Figure
g - gram
h - Hour
ha - Hectare
kg - kilogram
m - Meter
mg - Milli gram
ml - milliliter
mm - millimeter
no. - Number
viz. - Namely
°C - degree celsius
psi - pressure per square inch
LIST OF PLATES

Plate Page
Title
No. No.

I Pure cultures of 17 isolates of Trichoderma

II Pure cultures of bacterial isolates
Antagonistic activity of 17 isolates of Trichoderma
III
spp. against F. solani
Antagonistic activity of TCT and TC isolates of
4 10
IV
Trichoderma against F. solani
Antagonistic activity of bacterial isolate TCB (P )
1
V
against F. solani
Micro photographs with conidiophores, phialides and
VI
phialospores of Trichoderma isolate TCT
1
Micro photographs with conidiophores, phialides and
VII
phialospores of Trichoderma isolate TCT
2
Micro photographs with conidiophores, phialides and
VIII
phialospores of Trichoderma isolate TCT
3
Micro photographs with conidiophores, phialides and
IX
phialospores of Trichoderma isolate TCT
4
Micro photographs with conidiophores, phialides and
X
phialospores of Trichoderma isolate TCT
5
Micro photographs with conidiophores, phialides and
XI
phialospores of Trichoderma isolate TCT
6
Micro photographs with conidiophores, phialides and
XII
phialospores of Trichoderma isolate TCT
7
Micro photographs with conidiophores, phialides and
XIII
phialospores of Trichoderma isolate TCT
8
Micro photographs with conidiophores, phialides and
XIV
phialospores of Trichoderma isolate TCT
9
Micro photographs with conidiophores, phialides and
XV

phialospores of Trichoderma isolate TCT
10
Micro photographs with conidiophores, phialides and
XVI
phialospores of Trichoderma isolate TCT
11
Micro photographs with conidiophores, phialides and
XVII
phialospores of Trichoderma isolate TCT
12
Micro photographs with conidiophores, phialides and
XVIII
phialospores of Trichoderma isolate TCT
13
Micro photographs with conidiophores, phialides and
XIX
phialospores of Trichoderma isolate TCT
14
Micro photographs with conidiophores, phialides and
XX
phialospores of Trichoderma isolate TCT
15
Micro photographs with conidiophores, phialides and
XXI
phialospores of Trichoderma isolate TCT
16
Micro photographs with conidiophores, phialides and
XXII
phialospores of Trichoderma isolate TCT
17
Micro photographs with conidiophores, phialides and
XXIII
phialospores of Trichoderma viride
XXIV Protein profile of Trichoderma isolates by SDS-PAGE
XXV Protein profile of Trichoderma isolates by SDS-PAGE
XXVI Mass multiplication of Trichoderma isolate TCT4,
Pseudomonas isolate TCB(P ) and Fusarium solani
1
Efficacy of TCT , TCB(P ) and organic amendments
XXVII 4 1
in controlling dry root-rot disease