Searching and analysis of EST-SSR markers from linkage group 12 of the silkworm, Bombyx mori

2011·7.9 MB·

by  Zhi, Mi

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Te AU MLSE. ER MS pa, EAI EB aL | RACH 34
SOR OTR. Hole. TRS IDL RE Nat, ARIA EPS AT Le
PIU TLR, Sac 20 O28. ST ASBEPTAT Hel fe BET HEC EE
Bian ip, 2 ARLY BISHE SO), AGT ao AP HTL OLE | KESTATE A, LH pa
AAUP SH, WL F250 EP SUL U1 F27.S ip SUI 1S PISS MS I, AE
ARIA AR_AWANE: Wale by, oS We READ, LT MER I NE ERE
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RARE: Wes ARitaieh AGU, vistamecom: massa

Searching and analysis of EST-SSR markers from linkage group 12 of the
silkworm, Bombyx mori

MIZd, LI Dodie, LAN Crug-La, Li GuurNaa, DL Souls, LONG Perllane, ZUM You
(Collage af Bao arara oe, Santon Uninnat Changryg AN071 Ci

beim: Li alee bs eal dis plese

weal 16S epee smqunic tie CPST} oF Trkeaye ans 12 ofthe silkenira, Boose ear Mie
tilling end jetting, we obtained 58) naw-tsnadaat USLa vith de total leg about 40 Kb, A cota of
St ernple sere mate E

SSH oaslos ja elves, we scisved sad

ths weet is 122 ESTs shi erie a2, 786% a al a

fncacignted Ste, oneing ane FSIESEH jor 3,12 ban avenge, “Teveleaile pont ard ttamanlatie
rowing emit lagen. aie sumed fr 3 3846 nd 28.574 of ll ele HST
respectively, The areruge ‘orgth of mucostide: eps ue 16. 2 Up. amd the emcbnern ae 20 bp
Horas as moe: nf 26 FETs way een i MIB dene, which cto 4) 9S. Area
shen, SSRs {25.29} ms nl nthe STR, 1 SBR (28 $6) its 2 PTR an 1S SR

SS tle vag wseueuees {CUSR). Hho pans pace eae desig Ga ts woe EST-SSLe
aint ® of then shen pobemgp ie baad, Tie yriner FSI2A shar gly onnng stk
fseaias, Ue sills sageat Ua a Hate Wp He SK carers Ube gh seating of ikea
slants.

Kes words; Soom GomBys mor}: Linkage ou: expsceen! coqucuos tage ¢ STs

i Boni mort METRE, ANAM, SEH BS
AKG, A RSH RNID LRAEME — RAS LILA AT. Bonet, Rel
Hata EMES, OO NAY Lec sinple METTLE OTR UL28 AA,
sequence repeats, SSH MLM Ot Bee WISP RLURASUNS IS CR MAN PSM
aT Has Se ths Yer
SURAREMES | RAPH, SNR GUTS

FE a ek oN Ice, sym UD
FARR Pe, UU EE. ESP, Ue ARIE, t
“RC *
kas nase

SEWLE fs Min BOING OP
S516 PS A, ARS HH 9
#2 PLL
ral auch SOM AT SE
fa abals cSMAC, TTR,
MIE USER AAIP SW expreaed sequence ag;
AVERIANAPEIBIRE AR TEMEIRA, CINE
ERG CME, SAN Sa
«AMY EST E92, FHL ISU Meas UST-SSH
SSL LR PUT

FAT SSR GER

aa

Frade SER ARIEU, EA,

Fh, PT URACT PE SLRS, LUST
fi, Wi ac HSM

BERECT or, 20063 FSTSSR FRALIELLTIM,
Aa Bee. OR gh rR
SHTML, Solan ot wy 1999) HCL
SG 1Seom era 20005,, 11 HE; Condeao off, 2001)
AVE Poinsl ot alg 2001, 422 Hanh as
Welle. 20023, 2m). A.
anh. OE). ET Raley a, 2003s
Doo aT A

LUT
HI ldoulsbamersananin ct a,
(Sot af 2000 7 SHAD, RLY AAP RE
Do TCU We i OE, 2) HE
CRMS, ow), ECE, OUT YAN
‘ ARIAL (TST SOR
1 LALA,

hy 200) #5 1.26 1, NBL AEE
245 ODL See fe ESE H's

noe ot 263

bests
2S LAKIN Ta Ae ET
SERINE Del 2H SK BIE
S1212U1, 9121202, 91214 NSIS, FPA HE
es 2 HO we AEC,
FOR ISA P A) FST SSR AAT pw Hi.
PU SPCR RST ROAR, WHEE ATR
SI ie NH HOD TUES
sade

1 ee aR

La SSE 12H EST REN
SECACHE HL NC Ree uA aye

ves ri ihe neZBAST) ADP 205 9
SQE US. PINET HAL bad, DEER 2
ETOH AMELRUR Carp’ celUevor. eva. edu. cv
sil 305, SEHEIERE KE 250% I—Fiy 90%
AEST POISE 465 Be STM 33H 50 I
pale a sola Ji SMA: 1-200 bp A EST 9,
lila TAMIA. 2,9 LF TIMER ARTY SHAT AL
vek. ris SUAS I. nha
NEB eduussd Jy 20, AL SPA ina
SHUM RIAA CERT T:

UI sstluate Ke ARSE SEML. 2005,
POR A UBbsba, TEAR PSTs HET SSR Hes.
Pe M mR, IR. CIR. MET RR,
ERT QWAET ARS OMIM YL, 6.
4,3, 3 MSE, INGER SERV APE
Ae.
1.3 seme 2 sethaea SOR GmRRy ESI HH
WRK ARES

Fi MIASIA hp ws nis tw, go
eH SSR. A FST TATE
AE MSTA RC some) ALR LIF
‘identi Jc SS. EUR LST PAE
PERO SSR Sea RLS! TTR, TR CTS
ian
14 SHER 2 esier evr-ssn Bae
LAL Tell; MAIR LOTS RL de

(REF AIMEE PE Premine Poorer $73 AM
Feih, Ret: PEE TT 24 Up, PCH
BUDE AL 350 be
WE. CMY
epee AIRS AURAL al
14.2 GRAAL LAMAL NA HSER, ST
To, Joy TAL, 872, CIDR FT, IRV LT
JSPR TARA, RULSGE RMP
$1906) Pet SS Hale BZ TEAM DNA. Hy Sh
SeLAIAHMISE WHA JOD LS TU
SD NA Bead, BoA ORGS 10
Las Og Rabie sien. Pon REMC RS
1S pl TED CAE PUI buen, 2. auael LS
Mg!" 0,3 meet. BY AVIFE, 1 pal. 2031
OA TH Tay BA TH rg REE DA, POR
FA3Y ATRIA SACHS HSE
om RR. goes 2 ie rss RTI rs
nesses, tue 2, 90480 Wh, PGRN lp, HE vein
WoO. FUSE IPs 2k AI
2 BRSSHE Site SAFER A Ree, JE ALORA
AD FST SSR Sd +, SpAgF 122 SR RST RRIE,
21 maRa a aiNe EST oR EAT ee eee on

"Ve wae at
FETTDNAMAS 98.2.9 $c itabd des J FST
ete WUME, ARN SRL Se AE

293.12 Lb AYA) PSR, CLT
BME

Al son ARETE RST MIN
Table 1 The SSRs ofthe mwaednmdent EST to Bob act

a Ru IWR ENA) SRMRVAR
eH Test 7 26 68
“THA Dame 25 25.009
HH erent 4 ast a2

1 RAG ne SEIN ARIST BS, BMH — JOE ET B.S BR, Reape foment me

EAS HST-ASR Ani MMU TE, SA
Te aRE, ARE CHIE, FHT AES
SABIE, SE 96%, CUE ESE GAD
RAR 9. Gl. b35 SER BEANE 36. 6%
AEA RERE LE, MURETARIN 7.570:
FARR TER PT HO AURA
22.65% 2756410. M6, ATE
SI ULSUILAE 1.30%, dee
ESTES, WMCTR AN RE. HRT
MALIN, ADT-AAT Hem eC 7)
Pe RCS. GF A THRU RR
SET, TTA HBR CS PF LLEORRE SSR.
FH 16.0% 3)

Hie,

OLE SS
foe

NTE RARTINT
“ee zs a anil nies pn

2 FREE.

renee pets

21.2 ae 12 EALERTS EH

eB
ACUNMERES 12 HERUSY USTESSIE Hh, SDSL AL
ROM AREA RMN}, YEE BE

PULR, TRUSTS ROE, AEE
KHER 2. IRA TAL, IR
2), BF RAREST ARAMA ME
a REOMAE OM, cra, =
PAT, AT IL TOTAGA EMEA,
SUE AT AL ATS? SPR SER CUT He
SLAM RY. AT ROM IIE,
fe SHAT Bae IEE. 1
CR ERRtmAE. Thee 20K NSWAST hI A
4OP SSK, he 3s. 08 FF SL
(ESR GR STIR, IS NEAT S8 FF CTS
Me, Ae SUT LL, eT HAH AL,
16.2%, AMISH NIC ATR ART A SOLU SUI A, MARTI 7H Be
(2 op MARIE 20 Ip, EDEN ETEGERY COS ARAM. VRE ORD,
SERS. FUP RREE NY SSR Y PRET F ARSTCTR USIITR MS YARD T MLSE} SR
15 bp, TEAS HRT MOM 18 up, A AT ie Ta

WZ CTE, Wisk Cos

SBS SOU KNEE 20 bp, SS EWI ACAC/TIOA, SSR EE ORE,
21.3 BST T/T, SOR SA Hea GAIL Wyse->aHdA UE LAM AAR,
2 RR eH UST EDSSR DMEM
‘able 2 te tog sud moperts of re SKE af he Uoe-edaM ESP in Bombe wd
aah as TR LER FE pw ys
KR ma a Ana Be
1 Sinwenoe Bt ° 1 ‘
ABE co be ss . ‘
eH ede ne 2 ° .
3 OSU RRPETHENH SOR
[TAMeS The SSR dbtrthulln ak ten ea ol BST sense.
a ao ae, ET =
2.2 MSTSSR ASAE 4), RTM esr SER Atl wo aE

ATPL RSTSSC7 Oh ERP soT HE

PALM. =RTRE WMI E, UTES 13 TROT

FIM, FAA WERERS BERL STH JIT STS Nt ea Pn ST

Testis RRA) Som TOPS RSET OE S55 la
-QUCR [Ma 29-U RAED LHR. AT8—TSI2D1, FAIQO, SION AL RSI HELA HES!

MEM CETUS. ORME RR E SINS EV HUY HLA BGR 7a 902, ClM, abi

‘PCE KER PST-SSH IE STAD 4 fe SPCR, gf ean ENTSOK tet © NFL

weve A Weta, FS WT AV ESLNS (8

Ra HOD ER,
ir

De RAN PR Sag Te ENACT,

Fa REABRHq srs PER SmIMANR EER,

"he cin al ea of te ppnaepic He in ame or [ara sin} hy BESS mares

Fame Rae

rr

ANB:

ee EEE

Every

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Vig. 2 Tae ME omnes ea ih ra ISLS pines Hawa mow (Das esi

19 EAA OK ake

Fell Sibaoee BOTH 8 RR ARIE
ATU 2, UHR ET HO. RLS
POOLE ZEB, WIS SR CR,
AES CONS LAAT L Sea Tin T 92
P38. #1. PLLC MARES RSA TAL
SH MUNA LT, TENE | ATO WTR
TR FIAT EN PI he

WS AT clus rust TY

a ae

AMIE ICSE ART ACHP IEDRT BATSON
bh Set. SMBH (FST SSR ARERR
TONGA, RAB 12 TERS Slt PEST TEAL
Sandie
Fa
oe See Toe!

ble am ak nde ha

sat

eee ke Ty ae
ee S55 A HIB 85

MS 7 ay FOS signee GUE
otis ELEY moray 1 FER

ease

Bs TASS wT B
Ff MaPOR,

13 sa ob Sr
3 DNA FLAP INA ae LTE 2 Ba le 8B
a te Ny 5 5 458 CTH Skene

eee
Fsi5

LL SL Ee PST BA ATO HE
(RST RIE SN, SSR SP Beh PUNE
eR bs SBE, Pure

FRPFR, Winero EOWA NTT, OE
ASREITSNy, AEELTEE, MABE 22 4

GP ARAL DILA

Vanek (2001 i, wR TN
0 bp LOS SOR TL PAL,

SS AEA DN hp HLL Al dadeh RITES. HE

12 RHE ESSN IC eK tp
3.40, PTUTHERCH Sl rm
hs ATLMHLAT EH TE, “Fea

RAEN. iT ATW WATE
JOC, ATAAM RA TRANERS DS
A PRE aca aS SOR AIC A HE,
BOARS WOE 2 Hu, PAINS
EGULRAP BART). 10 797 FLICK Saha
SEM MLE RST-SSR fre i
RAT fh CHU. BEM ET.
ELI RATE AML,
aon

1 USTSSSUL SHIRE Je, CRAM 4 ah]
HiaMMI Aa As Paseo
BRAT PW RAM. Stites.
FORE ALG. MILL ALAC SRI 2 AA
(EHO, 10) aime 88 SETA TT
(625 102 APTLAATRA 350 HO SHETI Wi Mota a
2008), ER 12 SEAVER
STR OC, TREE a A

sr. $84
USFS ARR FT dee Has

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RO RE Heme, Coes a
Jug SC2WUS YF Chabane *& (2005 5
ir

fi. Aaja See Ah
RUT OMEMS Tk, BA HSA
TUM. 6 AST RTP IOTI 2.28 TE
SRHGUTOEAT, PRMEB a, Sag AETCIMNG)
ia

rit Smo

AREY Sas MARE, Se tea
HASH mina TNL AR

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kw ENTE tee Sages Sa He
eon wel “ ai beg Bs Mee
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