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Relationship between mixedfunction oxidases and the resistanceto fenvalerate in Helicoverpa armigera PDF

7 Pages·2001·0.3 MB·
by  Li-HongQiu
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Preview Relationship between mixedfunction oxidases and the resistanceto fenvalerate in Helicoverpa armigera

@C= 昆 虫 学 报 @@卷 表! 抗性和敏感种群棉铃虫乙氧试卤灵"#"脱乙基酶活性比较 $%&’(! )*+,%-./*0*123#4%56.7.6.(/&(68((0%1(07%’(-%6("-(/./6%06/6-%.0 %09%/:/5(,6.&’(/6-%.0*1 !; "#$%&’#" &’()[*+,-(. +"/·+0)] 组织!"##$% ’.1 敏感品系1$#2%*3"4-%#356"/ 抗性品系 ’%#"#36/3#356"/ 中肠+"70$3 89:;<=9>:6 ??9==<?98>2 @9?=! 脂肪体A634,7B =98C<=9=C4 ?9D=<=9==7 :9;=!! 体壁"/3%0$+%/3 =9?=<=9==4 =988<=9=@7 898=! 表< 抗性和敏感种群棉铃虫乙氧香豆素"#"脱乙基酶活性测定 $%&’(< )*+,%-./*0*12)#4%56.7.6.(/&(68((0%1(07%’(-%6("-(/./6%06/6-%.0 %09%/:/5(,6.&’(/6-%.0*1 !; "#$%&’#" EF()[/+,-(. +"/G +0)] 组织!"##$% ’.1 敏感品系1$#2%*3"4-%#356"/ 抗性品系 ’%#"#36/3#356"/ 中肠+"70$3 >9;H<=9886 ?=9>@<?9?D2 89::! 脂肪体A634,7B @9??<=9@=4 C9CH<=9?;7 ?9>:! 体壁"/3%0$+%/3 >9?@<=9>C6 @9?;<=9@?7 ?9>>! !=> 芳烷基羟基化酶(?@@)活性测定 以氧化的苯并芘表示IJJ活性(表@),结果表明抗氰戊菊酯种群棉铃虫中肠、脂肪体的 IJJ活性比敏感种群的显著提高(:9>=和@9=C倍),体壁的IJJ活性变化不显著。两种群中 脂肪体的IJJ活性均比中肠和体壁的明显提高,说明脂肪体的 &K(酶系更容易氧化降解苯 并芘。 !=A 艾氏剂环氧化酶(?2)活性测定 从表C 可以看出,抗氰戊菊酯种群的中肠、脂肪体的 IE活性和敏感种群的相比分别提 高89>@倍和?9@;倍,差异性极为显著,而体壁的IE活性则显著地比敏感种群的低(只有敏 感种群的C?L)。两种群中,不同组织部位微粒体对艾氏剂的环氧化能力均是脂肪体的最强, 中肠次之,体壁的最弱。 表> 抗性和敏感种群棉铃虫芳烷基羟基化酶活性比较 $%&’(> )*+,%-./*0*1?@@%56.7.6.(/&(68((0%1(07%’(-%6("-(/./6%06/6-%.0 %09%/:/5(,6.&’(/6-%.0/6-%.0/*1 !; "#$%&’#" 氧化苯并芘M(6)*[/+,-(. +"/·+0)] 组织!"##$% ’.1 敏感品系1$#2%*3"4-%#356"/ 抗性品系 ’%#"#36/3#356"/ 中肠+"70$3 ?9@C<=9=@6 H9?@<89:D6 :9>=!! 脂肪体A634,7B >89DH<89;>4 ?>89;D<889>>4 @9=C! 体壁"/3%0$+%/3 ;9C><=9>>2 :9;?<=9HC6 =9;=

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