Practical Thin-Layer Chromatography-A Multidisciplinary Approach PDF
Preview Practical Thin-Layer Chromatography-A Multidisciplinary Approach
PRACTICAL THIN-LAYER CHROMATOGRAPHY A Multidisciplinary Approach PRACTICAL THIN-LAYER CHROMATOGRAPHY A Multidisciplinary Approach Edited by Bernard Fried Joseph Sherma CRC Press Taylor Si Francis Group Boca Raton London New York CRC Press is an imprint of the Taylor & Francis Group, an informa business Acquiring Editor: Mark Dicker Project Editor; Les Kaplan Marketing Manager: Arline Massey Direct Marketing Manager: Becky McEldowney Cover design: Dawn Boyd PrePress: Kevin Luong Manufacturing: Sheri Schwartz Library of Congress Cataloging-in-Pubiication Data Practical thin-layer chromatography : a multidisciplinary approach ; edited by Bernard Fried, Joseph Sherma p. cm. Includes bibliographical references and index. ISBN 0-8493-2660-5 1. Thin-layer chromatography. I. Fried, Bernard, 1933-. II. Sherma, Joseph. H. QD79.C8P73 1996 543'.08956-dc20 DNLM/DLC 95-52348 CIP This book contains information obtained from authentic and highly regarded sources. Reprinted material is quoted with permission, and sources are indicated. A wide variety of references are listed. Reasonable efforts have been made to publish reliable data and information, but the author and the publisher cannot assume responsibility for the validity of all materials or for the consequences of their use. Neither this book nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, microfilming, and recording, or by any information storage or retrieval system, without prior permission in writing from the publisher. All rights reserved. Authorization to photocopy items for internal or personal use, or the personal or internal use of specific clients, may be granted by CRC Press, Inc., provided that $.50 per page photocopied is paid directly to Copyright Clearance Center, 27 Congress Street, Salem, MA 01970 USA. The fee code for users of the Transactional Reporting Service is ISBN 0-8493-2660-5/96/$0.00+$.50. The fee is subject to change without notice. For organizations that have been granted a photocopy license by the CCC, a separate system of payment has been arranged. The consent of CRC Press does not extend to copying for general distribution, for promotion, for creating new works, or for resale. Specific permission must be obtained in writing from CRC Press for such copying. Direct all inquiries to CRC Press, Inc., 2000 Corporate Blvd., N.W., Boca Raton, Florida 33431. © 1996 by CRC Press, Inc. No claim to original U.S. Government works International Standard Book Number 0-8493-2660-5 Library of Congress Card Number 95-52348 1 2 3 4 5 6 7 8 9 0 PREFACE After an introductory chapter on principles, techniques, and instrumentation, this book contains chapters that describe the use of thin-layer chromatography (TLC) in eleven important scientific disciplines. This approach is novel for books on TLC; most of them have a number of introductory chapters dealing with theory, practice, and instrumentation, followed by applications chapters organized according to com pound (analyte) type; contain a series of chapters on practice or applications; or are a random collection of papers from a symposium. The chapter authors comprise an international roster of expert practitioners of TLC in the most important application areas. We proposed this new disciplinary approach not only to avoid redundancy with existing books in the field but because we believe it provides thorough coverage of the principles, practices, and applications of TLC for important sample and compound types directed specifically at workers in the most active scientific fields. We attempted to make this book as practical as possible. Authors were encouraged to include the following topics in each chapter: an introduction that discusses the importance of TLC in the field and compares TLC to other analytical techniques; sample collection and preparation; chromatographic systems (layers and mobile phases); development techniques; detection, identification, and quantification of zones; validation of results; specific protocols for important analyses; and references through 1994. Chapters 2 to 6 cover fields of greatest interest to biologists and biochemists and Chapters 7 to 12 to chemists and analysts. Although the chapters were written by specialists in particular disciplines, the information presented is also applicable to analyses in related fields. The book should be useful to all scientists interested in the principles and practices of TLC. It will be of greatest interest to students, teachers, researchers, clinicians, and technicians who wish to apply TLC in the disciplines covered in the chapters and in related disciplines. We encourage readers to suggest ways to improve the book, including additional topics and authors, for the second edition. Bernard Fried Joseph Sherma Lafayette College, Easton, PA June, 1995 LIST OF CONTRIBUTORS Jan Bladek, Ph.D. M. A. Haseeb, Ph.D. Institute of Chemistry Department of Microbiology, Immunology and Military University of Technology Medicine Warsaw, Poland State University of New York Health Services Center Giuseppe Ceramelli, Ph.D. Brooklyn, New York Dipartimento Farmaco Chimico Technologico Università Degli Studi di Siena, Italy Raka Jain, Ph.D. Drug Dependence Treatment Centre All India Institute of Medical Sciences Pietro Corti, Ph.D. New Delhi, India Dipartimento Farmaco Chimico Technologico Università Degli Studi di Siena, Italy Mark Maloney, Ph.D. Biology Department Tibot Cserhàti, Ph.D. Spelman College Central Research Institute of Chemistry Atlanta, Georgia Hungarian Academy of Sciences Budapest, Hungary Illka Ojanpera, Ph.D. Forensic Toxicology Division Elena Dreassi, Ph.D. Department of Forensic Medicine Dipartimento Farmaco Chimico Technologico University of Helsinki Università Degli Studi di Siena, Italy Helsinki, Finland Richard D. Fell, Ph.D. Ali R. Shalaby, Ph.D. Department of Entomology Food Technology and Dairy Sciences Department Virginia Polytechnic Institute and State University National Research Centre Blacksburg, Virginia Cairo, Egypt Esther Forgacs, Ph.D. H. M. Stahr, Ph J). Central Research Institute of Chemistry College of Veterinary Medicine Hungarian Academy of Sciences University of Iowa Budapest, Hungary Ames, Iowa Bernard Fried, Ph.D. Paul J. Weldon, Ph.D. Department of Biology Conservation and Research Center Lafayette University Smithsonian Institute Easton, Pennsylvania Front Royal, Virginia CONTENTS Chapter 1 Introduction to Techniques and Instrumentation..................................................................................................1 Tibor Cserhati and Ester Forgacs Chapter 2 Thin-Layer Chromatography in Bacteriology...................................................................................................19 Mark Maloney Chapter 3 Thin-Layer Chromatography in Plant Sciences................................................................................................33 Jacques Pothier Chapter 4 Thin-Layer Chromatography in Parasitology...................................................................................................51 Bernard Fried Chapter 5 Thin-Layer Chromatography in Studies in Entomology.................................................................................71 Richard D. Fell Chapter 6 Thin-Layer Chromatography of Skin Secretions in Vertebrates..................................................................105 Paul Weldon Chapter 7 Thin-Layer Chromatography in Clinical Chemistry......................................................................................131 Raka Jain Chapter 8 Thin-Layer Chromatography in Environmental Analysis.............................................................................153 Jan Bladek Chapter 9 Thin-Layer Chromatography in Food Analysis..............................................................................................169 Ali R. Shalaby Chapter 10 Thin-Layer Chromatography in Forensic Toxicology....................................................................................193 Ilkka Ojanpera Chapter 11 Thin-Layer Chromatography in Pharmaceutical Analysis............................................................................231 Flena Dreassi, Giuseppe Ceramelli, and Pietro Corti Chapter 12 Thin-Layer Chromatography in Veterinary Toxicology.................................... 249 H. M. Stahr Index...........................................................................................................................................................................265
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