Pathology of the developing mouse : a systematic approach

442 Pages·2015·102.26 MB·English

by  Brad Bolon

Animal Science BOLON
Pathology of the Developing Mouse
A Systematic Approach
Pathology of the Developing Mouse: A Systematic Approach is the first publication to provide
comprehensive guidance regarding the identification, characterization, and interpretation of mouse
developmental defects. Emphasizing the interpretation of abnormal phenotypes, it provides detailed P
protocols for appropriately processing mouse embryos, fetuses, neonates, juveniles, and placentas a
as well as recognizing and categorizing structural lesions. t
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Topics include:
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o
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• Baseline anatomic and physiologic traits of developing mice: embryos, fetuses,
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neonates, and juveniles

• Comprehensive developmental timelines for major organs and systems o
• Principles of good experimental design and statistical analysis for mouse f

developmental pathology studies t
• Procedures for anatomic pathology examinations to evaluate structural changes h
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at the macroscopic, microscopic, and ultrastructural levels, using conventional

autopsy-based or novel noninvasive imaging techniques D
• Methods for clinical pathology testing to assess biochemical and cellular e
composition of tissues and fluids v
e
• Options and protocols for in situ molecular pathology analysis for site-specific
l
explorations of various mechanisms responsible for producing adverse findings o
during development p
• Basic principles for interpreting anatomic pathology and clinical pathology i
n
changes in the animal and placenta Pathology of the
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A complete reference on the design, analysis, and interpretation of abnormal findings in developing M
mice, the book is enhanced with abundant full-color illustrations, enabling readers to comprehend o
u
both normal development and the major categories of defects that may be seen in malformed
s
embryos, fetuses, neonates, juveniles, and placentas. The numerous literature citations provide an
e
excellent starting point for additional reading to begin building true expertise in the field. The
comprehensive coverage of the topic will make this book a regularly referenced developmental Developing Mouse
pathology resource in basic and biomedical research laboratories around the world.
A Systematic Approach
70088
ISBN: 978-1-4200-7008-8
90000
Brad Bolon
Edited by
9 781420 070088

Pathology of the
Developing Mouse

Pathology of the
Developing Mouse
A Systematic Approach
Brad Bolon
Edited by
Boca Raton London New York
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Version Date: 20141017
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This book is dedicated to those hardy souls
who seek understanding of large truths from small canvases,
who aspire to discover universal principles within ever-changing flesh,
who strive to build a healthier tomorrow for all by willingly
paying the price of difficult learning today.
In other words, this book is devoted to all of you
who have already suffered, or will someday encounter,
the delights and debacles of mouse developmental pathology.

Contents
Preface ......................................................................................................................................................ix
Acknowledgments .....................................................................................................................................xi
Editor ......................................................................................................................................................xiii
Contributors .............................................................................................................................................xv
Section I Introduction
1. The Case for Developmental Pathology and Developmental Pathologists .................................3
Brad Bolon
Section II Fundamentals of Mouse Developmental Biology
2. Essentials of Mouse Genetics and Nomenclature........................................................................13
Oded Foreman and Brad Bolon
3. Essential Terminology for Mouse Developmental Pathology Studies .......................................27
Brad Bolon and Colin G. Rousseaux
4. Anatomy and Physiology of the Developing Mouse and Placenta .............................................39
Brad Bolon and Jerrold M. Ward
5. Teratogens and Teratogenic Mechanisms in Mouse Developmental Pathology ......................99
Brad Bolon and Colin G. Rousseaux
Section III Experimental Methods in Mouse Developmental Pathology
6. Principles of Experimental Design for Mouse Developmental Pathology Studies .................117
Brad Bolon and Krista M.D. La Perle
7. Necropsy Sampling and Data Collection for Studying the Anatomy, Histology, and
Pathology of Mouse Development ................................................................................................133
Susan Newbigging, Jerrold M. Ward, and Brad Bolon
8. Clinical Pathology Analysis in Developing Mice ........................................................................175
Kelli L. Boyd, Brad Bolon, and Denise I. Bounous
9. Histotechnological Processing of Developing Mice ...................................................................195
Brad Bolon, Diane Duryea, and Julie F. Foley
10. Localization of Gene Expression in Developing Mice ...............................................................211
Jennifer M. Mele and Brad Bolon
vii
viii Contents
11. Protein Localization in Developing Mice ...................................................................................227
Brad Bolon and Efrain Pacheco
12. Quantitative Morphological Assessment in Mouse Developmental Pathology Studies ........245
Brad Bolon and Stephen Kaufman
13. Ultrastructural Evaluation of Developing Mice ........................................................................259
Brad Bolon, Stephen Kaufman, and Richard Montione
14. Three-Dimensional Imaging in Mouse Developmental Pathology Studies ............................275
Brad Bolon, Kathleen Gabrielson, Sara Cole, Olga N. Kovbasnjuk, Kimerly A. Powell,
and David Weinstein
Section IV Analytical Practices for Mouse Developmental Pathology
15. Pathology of the Developing Mouse from Conception to Weaning .........................................293
Brad Bolon and Vinicius Carreira
16. Pathology of the Placenta .............................................................................................................355
Brad Bolon and Jerrold M. Ward
17. Mouse Developmental Pathology Assessments in High-Throughput
Phenogenomic Facilities ...............................................................................................................377
Colin McKerlie, Susan Newbigging, and Geoffrey A. Wood
18. A Statistical Analysis Primer for Mouse Developmental Pathology Studies .........................405
Brad Bolon and Colin G. Rousseaux
Appendix: Common Abbreviations in Mouse Developmental Pathology Studies..........................421