Patch Clamp Electrophysiology: Methods and Protocols

333 Pages·2021·9.696 MB·English

by  Mark Dallas, Damian Bell

Methods in
Molecular Biology 2188
Mark Dallas
Damian Bell Editors
Patch Clamp
Electro-
physiology
Methods and Protocols
M M B
ETHODS IN OLECULAR IO LO GY
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School of Lifeand MedicalSciences
University ofHertfordshire
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Patch Clamp Electrophysiology
Methods and Protocols
Edited by
Mark Dallas
University of Reading, Reading, UK
Damian Bell
Sophion Bioscience A/S, Ballerup, Denmark
Editors
MarkDallas DamianBell
UniversityofReading SophionBioscienceA/S
Reading,UK Ballerup,Denmark
ISSN1064-3745 ISSN1940-6029 (electronic)
MethodsinMolecularBiology
ISBN978-1-0716-0817-3 ISBN978-1-0716-0818-0 (eBook)
https://doi.org/10.1007/978-1-0716-0818-0
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Preface
Theflowofionsacrosscellularmembraneshasintriguedresearchersfordecades,andassuch
therehasbeenacontinualquesttodevelopandimproveamethodologytounderstandthe
regulatory mechanisms underpinning ionic homeostasis. It is vital that we understand the
role that ionic fluxes play in cellular physiology, given the pathological consequences of
uncontrolledionicflux.Centraltothiscontrolareionchannelsthatpresentalargearrayof
protein superfamilies that have been studied extensively to probe their structure and
function.Thedevelopmentofthepatchclamptechniquein1981byHamillandcolleagues
providedourfirstinsightintothe“real-time”functioningoftheseionchannelsandwithit
theabilitytodefinetheirbiophysicsandpharmacology.Indeed,theoriginalsingle-channel
work by Neher and Sakmann featured in Nature’s 10 extraordinary papers. Since this
seminal work, the ion channel field has grown exponentially with the addition of new
technologiessupportingourevolvingideasabout“howionchannelswork.”
Thisvolumeprovidesaninvaluableupdatebydescribingarangeofnovelmethodologi-
cal approaches that have been developed to probe ion channel function across different
modalities. These approaches have been applied to distinct channels or indeed distinct
research areas, often transferable across the vast vistas of ion channel and transporter
families. Here a global collection of experts share their substantial practical experience
overawidespanofmethods,fromabasicintroductiontopatchclampfor theionchannel
neophyte to more complex and bleeding-edge experimental protocols, including optoge-
netics.Naturally, the field ofion channel methodologyis extensive,and thereare ahost of
valuable sources to use to support this volume. We would signpost the reader to use Ionic
ChannelsofExcitableMembranes(Hille)andMicroelectrodeTechniques:ThePlymouthWork-
shop Handbook (http://plymsea.ac.uk/id/eprint/7954/) as complementary texts. Our
focus of this volume was to add to these and provide a timely update to existing books
and articles, building on these foundations to hopefully become a routine reference guide
forcurrentandfutureionchannelphysiologists.
Researchers from a wide range of disciplines will benefit from this volume, which sets
out to simplify the perceivedcomplexities of electrophysiology and providesome advances
in methods for more experienced ion channel researchers. With an increasing demand for
thesubmissionofcompletestoriestojournalsandfundingbodies,thescopeforinclusionof
these highly technical procedures is becoming a fundamental part of scientific enquiry. We
hopeyoufindthisausefuladditiontotheionchannelliteraturecanon.
Reading,UK MarkDallas
Ballerup,Denmark DamianBell
v
Contents
Preface ..................................................................... v
Contributors................................................................. ix
1 AnIntroductiontoPatchClampRecording................................ 1
CharlotteL.HillandGaryJ.Stephens
2 PatchClampTechnologyintheTwenty-FirstCentury ...................... 21
JanDolzer
3 HeterologousExpressionofIonChannelsinMammalianCellLines .......... 51
AlistairMathie,EmmaL.Veale,andRobynG.Holden
4 ElectrophysiologyonChannel-FormingProteinsinArtificial
LipidBilayers:Next-GenerationInstrumentationforMultiple
RecordingsinParallel ................................................... 67
EkaterinaZaitseva,AlisonObergrussberger,ConradWeichbrodt,
MordjaneBoukhet,FrankBernhard,ChristopherHein,GerhardBaaken,
NielsFertig,andJanC.Behrends
5 PerforatedWhole-CellRecordingsinAutomatedPatchClamp
Electrophysiology....................................................... 93
KadlaR.Rosholm,KimBoddum,andAndersLindquist
6 MultielectrodeArrays.................................................... 109
RussellBurleyandJennaR.M.Harvey
7 UtilisingAutomatedElectrophysiologicalPlatformsinEpilepsyResearch...... 133
CarolJ.MilliganandSvenjaPachernegg
8 DynamicClamponaWindowsPC........................................ 157
LorinS.MilescuandJo¨elTabak
9 CombiningWhole-CellPatch-ClampRecordingswithSingle-Cell
RNASequencing ....................................................... 179
KashifMahfoozandTommasJ.Ellender
10 BioluminescenceMethodologyforIonChannelStudies..................... 191
PaulA.Wadsworth,AdityaK.Singh,NghiNguyen,
CliffordStephan,andFernandaLaezza
11 Nucleated,Outside-Out,Somatic,MacropatchRecordings
inNativeNeurons ...................................................... 229
FrancescoTamagnini
12 PreparationofRatOrganotypicHippocampalSliceCultures
UsingtheMembrane-InterfaceMethod................................... 243
TimothyW.ChurchandMatthewG.Gold
13 InVivoPatch-ClampStudies............................................. 259
YiZhou,HeLi,andZhongjuXiao
14 InVivoOptogeneticswithStimulusCalibration............................ 273
LukeT.CoddingtonandJoshuaT.Dudman
vii
viii Contents
15 SingleorDoublePatch-ClampRecordingsInExVivoSlice
Preparation:FunctionalConnectivity,SynapseDynamics,
andOptogenetics....................................................... 285
JeanSimonnet,LouisRichevaux,andDesdemonaFricker
16 OptogeneticsandOpticalToolsinAutomatedPatchClamping .............. 311
KimBoddum,PederSkafte-Pedersen,Jean-FrancoisRolland,
andSandraWilson
Index ...................................................................... 331
Contributors
GERHARDBAAKEN • IoneraTechnologiesGmbH,Freiburg,Germany
JANC.BEHRENDS • LaboratoryforMembranePhysiologyandTechnology,Departmentof
Physiology,FacultyofMedicine,UniversityofFreiburg,Freiburg,Germany
FRANKBERNHARD • InstituteofBiophysicalChemistry&Center forBiomolecularMagnetic
Resonance,GoetheUniversityFrankfurt,FrankfurtamMain,Germany
KIMBODDUM • SophionBioscienceA/S,Ballerup,Denmark
MORDJANEBOUKHET • IoneraTechnologiesGmbH,Freiburg,Germany
RUSSELL BURLEY • CerevanceLtd.,Cambridge,UK
TIMOTHYW.CHURCH • DepartmentofNeuroscience,PhysiologyandPharmacology,
UniversityCollegeLondon,London,UK
LUKET.CODDINGTON • JaneliaResearchCampus,HowardHughesMedicalInstitute,
Ashburn,VA,USA
JANDOLZER • SutterInstrumentCompany,Novato,CA,USA
JOSHUAT.DUDMAN • JaneliaResearchCampus,HowardHughesMedicalInstitute,
Ashburn,VA,USA
TOMMASJ.ELLENDER • DepartmentofPharmacology,UniversityofOxford,Oxford,UK
NIELSFERTIG • NanionTechnologiesGmbH,Munich,Germany
DESDEMONAFRICKER • CNRS(IntegrativeNeuroscienceandCognitionCenter,UMR
8002),Paris,France;Universite´deParis,Paris,France
MATTHEWG.GOLD • DepartmentofNeuroscience,PhysiologyandPharmacology,University
CollegeLondon,London,UK
JENNAR.M.HARVEY • CerevanceLtd.,Cambridge,UK
CHRISTOPHERHEIN • InstituteofBiophysicalChemistry&CenterforBiomolecularMagnetic
Resonance,GoetheUniversityFrankfurt,FrankfurtamMain,Germany
CHARLOTTEL.HILL • MetrionBiosciencesLtd,Cambridge,UK
ROBYNG.HOLDEN • MedwaySchoolofPharmacy,UniversityofKentandUniversityof
Greenwich,ChathamMaritime,UK
FERNANDALAEZZA • DepartmentofPharmacologyandToxicology,TheUniversityofTexas
MedicalBranch,Galveston,TX,USA
HELI • DepartmentofPhysiology,SouthernMedicalUniversity,Guangzhou,Guangdong,
China
ANDERSLINDQUIST • SophionBioscienceA/S,Ballerup,Denmark
KASHIF MAHFOOZ • DepartmentofPharmacology,UniversityofOxford,Oxford,UK
ALISTAIRMATHIE • MedwaySchoolofPharmacy,UniversityofKentandUniversityof
Greenwich,ChathamMaritime,UK
LORINS.MILESCU • DepartmentofBiology,UniversityofMarylandatCollegePark,College
Park,MD,USA
CAROLJ.MILLIGAN • FloreyInstituteofNeuroscienceandMentalHealth,MelbourneBrain
Centre,Parkville,VIC,Australia
NGHINGUYEN • HTSScreeningCore,Center forTranslationalCancerResearch,Texas
A&MHealthScienceCenter:InstituteofBiosciencesandTechnology,Houston,TX,USA
ALISONOBERGRUSSBERGER • NanionTechnologiesGmbH,Munich,Germany
ix
x Contributors
SVENJAPACHERNEGG • FloreyInstituteofNeuroscienceandMentalHealth,MelbourneBrain
Centre,Parkville,VIC,Australia;ReproductiveDevelopmentGroup,MurdochChildren’s
ResearchInstitute,Parkville,VIC,Australia
LOUISRICHEVAUX • CNRS(IntegrativeNeuroscienceandCognitionCenter,UMR8002),
Paris,France;Universite´deParis,Paris,France
JEAN-FRANCOISROLLAND • AxxamSpA,Milan,Italy
KADLA R.ROSHOLM • SophionBioscienceA/S,Ballerup,Denmark
JEANSIMONNET • BernsteinCenterforComputationalNeuroscience,Humboldt-Universita€t
zuBerlin,Berlin,Germany
ADITYAK.SINGH • DepartmentofPharmacologyandToxicology,TheUniversityofTexas
MedicalBranch,Galveston,TX,USA
PEDERSKAFTE-PEDERSEN • SophionBioscienceA/S,Ballerup,Denmark
CLIFFORDSTEPHAN • HTSScreeningCore,Center forTranslationalCancerResearch,Texas
A&MHealthScienceCenter:InstituteofBiosciencesandTechnology,Houston,TX,USA
GARYJ.STEPHENS • ReadingSchoolofPharmacy,UniversityofReading,Berkshire,UK
JOE¨LTABAK • InstituteofBiomedicalandClinicalScience,UniversityofExeterMedical
School,Exeter,Devon,UK
FRANCESCOTAMAGNINI • SchoolofPharmacy,UniversityofReading,WhiteknightsCampus,
Reading,UK
EMMA L.VEALE • MedwaySchoolofPharmacy,UniversityofKentandUniversityof
Greenwich,ChathamMaritime,UK
PAULA.WADSWORTH • BiochemistryandMolecularBiologyGraduateProgram,The
UniversityofTexasMedicalBranch,Galveston,TX,USA;DepartmentofPharmacology
andToxicology,TheUniversityofTexasMedicalBranch,Galveston,TX,USA
CONRADWEICHBRODT • NanionTechnologiesGmbH,Munich,Germany
SANDRAWILSON • SophionBioscienceA/S,Ballerup,Denmark
ZHONGJU XIAO • DepartmentofPhysiology,SouthernMedicalUniversity,Guangdong,
Guangzhou,China
EKATERINAZAITSEVA • IoneraTechnologiesGmbH,Freiburg,Germany
YIZHOU • DepartmentofNeurobiology,SchoolofBasicMedicalSciences,Army Medical
University,Chongqing,China