Patch Clamp Electrophysiology: Methods and Protocols PDF
Preview Patch Clamp Electrophysiology: Methods and Protocols
Methods in Molecular Biology 2188 Mark Dallas Damian Bell Editors Patch Clamp Electro- physiology Methods and Protocols M M B ETHODS IN OLECULAR IO LO GY SeriesEditor JohnM.Walker School of Lifeand MedicalSciences University ofHertfordshire Hatfield, Hertfordshire, UK Forfurther volumes: http://www.springer.com/series/7651 For over 35 years, biological scientists have come to rely on the research protocols and methodologiesinthecriticallyacclaimedMethodsinMolecularBiologyseries.Theserieswas thefirsttointroducethestep-by-stepprotocolsapproachthathasbecomethestandardinall biomedicalprotocolpublishing.Eachprotocolisprovidedinreadily-reproduciblestep-by- step fashion, opening with an introductory overview, a list of the materials and reagents neededtocompletetheexperiment,andfollowedbyadetailedprocedurethatissupported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. These hallmark features were introduced by series editor Dr. John Walker and constitutethekeyingredientineachandeveryvolumeoftheMethodsinMolecularBiology series. Tested and trusted, comprehensive and reliable, all protocols from the series are indexedinPubMed. Patch Clamp Electrophysiology Methods and Protocols Edited by Mark Dallas University of Reading, Reading, UK Damian Bell Sophion Bioscience A/S, Ballerup, Denmark Editors MarkDallas DamianBell UniversityofReading SophionBioscienceA/S Reading,UK Ballerup,Denmark ISSN1064-3745 ISSN1940-6029 (electronic) MethodsinMolecularBiology ISBN978-1-0716-0817-3 ISBN978-1-0716-0818-0 (eBook) https://doi.org/10.1007/978-1-0716-0818-0 ©SpringerScience+BusinessMedia,LLC,partofSpringerNature2021 Thisworkissubjecttocopyright.AllrightsarereservedbythePublisher,whetherthewholeorpartofthematerialis concerned,specificallytherightsoftranslation,reprinting,reuseofillustrations,recitation,broadcasting,reproduction onmicrofilmsorinanyotherphysicalway,andtransmissionorinformationstorageandretrieval,electronicadaptation, computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdeveloped. Theuseofgeneraldescriptivenames,registerednames,trademarks,servicemarks,etc.inthispublicationdoesnotimply, evenintheabsenceofaspecificstatement,thatsuchnamesareexemptfromtherelevantprotectivelawsandregulations andthereforefreeforgeneraluse. Thepublisher,theauthors,andtheeditorsaresafetoassumethattheadviceandinformationinthisbookarebelievedto betrueandaccurateatthedateofpublication.Neitherthepublishernortheauthorsortheeditorsgiveawarranty, expressedorimplied,withrespecttothematerialcontainedhereinorforanyerrorsoromissionsthatmayhavebeen made.Thepublisherremainsneutralwithregardtojurisdictionalclaimsinpublishedmapsandinstitutionalaffiliations. ThisHumanaimprintispublishedbytheregisteredcompanySpringerScience+BusinessMedia,LLC,partofSpringer Nature. Theregisteredcompanyaddressis:1NewYorkPlaza,NewYork,NY10004,U.S.A. Preface Theflowofionsacrosscellularmembraneshasintriguedresearchersfordecades,andassuch therehasbeenacontinualquesttodevelopandimproveamethodologytounderstandthe regulatory mechanisms underpinning ionic homeostasis. It is vital that we understand the role that ionic fluxes play in cellular physiology, given the pathological consequences of uncontrolledionicflux.Centraltothiscontrolareionchannelsthatpresentalargearrayof protein superfamilies that have been studied extensively to probe their structure and function.Thedevelopmentofthepatchclamptechniquein1981byHamillandcolleagues providedourfirstinsightintothe“real-time”functioningoftheseionchannelsandwithit theabilitytodefinetheirbiophysicsandpharmacology.Indeed,theoriginalsingle-channel work by Neher and Sakmann featured in Nature’s 10 extraordinary papers. Since this seminal work, the ion channel field has grown exponentially with the addition of new technologiessupportingourevolvingideasabout“howionchannelswork.” Thisvolumeprovidesaninvaluableupdatebydescribingarangeofnovelmethodologi- cal approaches that have been developed to probe ion channel function across different modalities. These approaches have been applied to distinct channels or indeed distinct research areas, often transferable across the vast vistas of ion channel and transporter families. Here a global collection of experts share their substantial practical experience overawidespanofmethods,fromabasicintroductiontopatchclampfor theionchannel neophyte to more complex and bleeding-edge experimental protocols, including optoge- netics.Naturally, the field ofion channel methodologyis extensive,and thereare ahost of valuable sources to use to support this volume. We would signpost the reader to use Ionic ChannelsofExcitableMembranes(Hille)andMicroelectrodeTechniques:ThePlymouthWork- shop Handbook (http://plymsea.ac.uk/id/eprint/7954/) as complementary texts. Our focus of this volume was to add to these and provide a timely update to existing books and articles, building on these foundations to hopefully become a routine reference guide forcurrentandfutureionchannelphysiologists. Researchers from a wide range of disciplines will benefit from this volume, which sets out to simplify the perceivedcomplexities of electrophysiology and providesome advances in methods for more experienced ion channel researchers. With an increasing demand for thesubmissionofcompletestoriestojournalsandfundingbodies,thescopeforinclusionof these highly technical procedures is becoming a fundamental part of scientific enquiry. We hopeyoufindthisausefuladditiontotheionchannelliteraturecanon. Reading,UK MarkDallas Ballerup,Denmark DamianBell v Contents Preface ..................................................................... v Contributors................................................................. ix 1 AnIntroductiontoPatchClampRecording................................ 1 CharlotteL.HillandGaryJ.Stephens 2 PatchClampTechnologyintheTwenty-FirstCentury ...................... 21 JanDolzer 3 HeterologousExpressionofIonChannelsinMammalianCellLines .......... 51 AlistairMathie,EmmaL.Veale,andRobynG.Holden 4 ElectrophysiologyonChannel-FormingProteinsinArtificial LipidBilayers:Next-GenerationInstrumentationforMultiple RecordingsinParallel ................................................... 67 EkaterinaZaitseva,AlisonObergrussberger,ConradWeichbrodt, MordjaneBoukhet,FrankBernhard,ChristopherHein,GerhardBaaken, NielsFertig,andJanC.Behrends 5 PerforatedWhole-CellRecordingsinAutomatedPatchClamp Electrophysiology....................................................... 93 KadlaR.Rosholm,KimBoddum,andAndersLindquist 6 MultielectrodeArrays.................................................... 109 RussellBurleyandJennaR.M.Harvey 7 UtilisingAutomatedElectrophysiologicalPlatformsinEpilepsyResearch...... 133 CarolJ.MilliganandSvenjaPachernegg 8 DynamicClamponaWindowsPC........................................ 157 LorinS.MilescuandJo¨elTabak 9 CombiningWhole-CellPatch-ClampRecordingswithSingle-Cell RNASequencing ....................................................... 179 KashifMahfoozandTommasJ.Ellender 10 BioluminescenceMethodologyforIonChannelStudies..................... 191 PaulA.Wadsworth,AdityaK.Singh,NghiNguyen, CliffordStephan,andFernandaLaezza 11 Nucleated,Outside-Out,Somatic,MacropatchRecordings inNativeNeurons ...................................................... 229 FrancescoTamagnini 12 PreparationofRatOrganotypicHippocampalSliceCultures UsingtheMembrane-InterfaceMethod................................... 243 TimothyW.ChurchandMatthewG.Gold 13 InVivoPatch-ClampStudies............................................. 259 YiZhou,HeLi,andZhongjuXiao 14 InVivoOptogeneticswithStimulusCalibration............................ 273 LukeT.CoddingtonandJoshuaT.Dudman vii viii Contents 15 SingleorDoublePatch-ClampRecordingsInExVivoSlice Preparation:FunctionalConnectivity,SynapseDynamics, andOptogenetics....................................................... 285 JeanSimonnet,LouisRichevaux,andDesdemonaFricker 16 OptogeneticsandOpticalToolsinAutomatedPatchClamping .............. 311 KimBoddum,PederSkafte-Pedersen,Jean-FrancoisRolland, andSandraWilson Index ...................................................................... 331 Contributors GERHARDBAAKEN • IoneraTechnologiesGmbH,Freiburg,Germany JANC.BEHRENDS • LaboratoryforMembranePhysiologyandTechnology,Departmentof Physiology,FacultyofMedicine,UniversityofFreiburg,Freiburg,Germany FRANKBERNHARD • InstituteofBiophysicalChemistry&Center forBiomolecularMagnetic Resonance,GoetheUniversityFrankfurt,FrankfurtamMain,Germany KIMBODDUM • SophionBioscienceA/S,Ballerup,Denmark MORDJANEBOUKHET • IoneraTechnologiesGmbH,Freiburg,Germany RUSSELL BURLEY • CerevanceLtd.,Cambridge,UK TIMOTHYW.CHURCH • DepartmentofNeuroscience,PhysiologyandPharmacology, UniversityCollegeLondon,London,UK LUKET.CODDINGTON • JaneliaResearchCampus,HowardHughesMedicalInstitute, Ashburn,VA,USA JANDOLZER • SutterInstrumentCompany,Novato,CA,USA JOSHUAT.DUDMAN • JaneliaResearchCampus,HowardHughesMedicalInstitute, Ashburn,VA,USA TOMMASJ.ELLENDER • DepartmentofPharmacology,UniversityofOxford,Oxford,UK NIELSFERTIG • NanionTechnologiesGmbH,Munich,Germany DESDEMONAFRICKER • CNRS(IntegrativeNeuroscienceandCognitionCenter,UMR 8002),Paris,France;Universite´deParis,Paris,France MATTHEWG.GOLD • DepartmentofNeuroscience,PhysiologyandPharmacology,University CollegeLondon,London,UK JENNAR.M.HARVEY • CerevanceLtd.,Cambridge,UK CHRISTOPHERHEIN • InstituteofBiophysicalChemistry&CenterforBiomolecularMagnetic Resonance,GoetheUniversityFrankfurt,FrankfurtamMain,Germany CHARLOTTEL.HILL • MetrionBiosciencesLtd,Cambridge,UK ROBYNG.HOLDEN • MedwaySchoolofPharmacy,UniversityofKentandUniversityof Greenwich,ChathamMaritime,UK FERNANDALAEZZA • DepartmentofPharmacologyandToxicology,TheUniversityofTexas MedicalBranch,Galveston,TX,USA HELI • DepartmentofPhysiology,SouthernMedicalUniversity,Guangzhou,Guangdong, China ANDERSLINDQUIST • SophionBioscienceA/S,Ballerup,Denmark KASHIF MAHFOOZ • DepartmentofPharmacology,UniversityofOxford,Oxford,UK ALISTAIRMATHIE • MedwaySchoolofPharmacy,UniversityofKentandUniversityof Greenwich,ChathamMaritime,UK LORINS.MILESCU • DepartmentofBiology,UniversityofMarylandatCollegePark,College Park,MD,USA CAROLJ.MILLIGAN • FloreyInstituteofNeuroscienceandMentalHealth,MelbourneBrain Centre,Parkville,VIC,Australia NGHINGUYEN • HTSScreeningCore,Center forTranslationalCancerResearch,Texas A&MHealthScienceCenter:InstituteofBiosciencesandTechnology,Houston,TX,USA ALISONOBERGRUSSBERGER • NanionTechnologiesGmbH,Munich,Germany ix x Contributors SVENJAPACHERNEGG • FloreyInstituteofNeuroscienceandMentalHealth,MelbourneBrain Centre,Parkville,VIC,Australia;ReproductiveDevelopmentGroup,MurdochChildren’s ResearchInstitute,Parkville,VIC,Australia LOUISRICHEVAUX • CNRS(IntegrativeNeuroscienceandCognitionCenter,UMR8002), Paris,France;Universite´deParis,Paris,France JEAN-FRANCOISROLLAND • AxxamSpA,Milan,Italy KADLA R.ROSHOLM • SophionBioscienceA/S,Ballerup,Denmark JEANSIMONNET • BernsteinCenterforComputationalNeuroscience,Humboldt-Universita€t zuBerlin,Berlin,Germany ADITYAK.SINGH • DepartmentofPharmacologyandToxicology,TheUniversityofTexas MedicalBranch,Galveston,TX,USA PEDERSKAFTE-PEDERSEN • SophionBioscienceA/S,Ballerup,Denmark CLIFFORDSTEPHAN • HTSScreeningCore,Center forTranslationalCancerResearch,Texas A&MHealthScienceCenter:InstituteofBiosciencesandTechnology,Houston,TX,USA GARYJ.STEPHENS • ReadingSchoolofPharmacy,UniversityofReading,Berkshire,UK JOE¨LTABAK • InstituteofBiomedicalandClinicalScience,UniversityofExeterMedical School,Exeter,Devon,UK FRANCESCOTAMAGNINI • SchoolofPharmacy,UniversityofReading,WhiteknightsCampus, Reading,UK EMMA L.VEALE • MedwaySchoolofPharmacy,UniversityofKentandUniversityof Greenwich,ChathamMaritime,UK PAULA.WADSWORTH • BiochemistryandMolecularBiologyGraduateProgram,The UniversityofTexasMedicalBranch,Galveston,TX,USA;DepartmentofPharmacology andToxicology,TheUniversityofTexasMedicalBranch,Galveston,TX,USA CONRADWEICHBRODT • NanionTechnologiesGmbH,Munich,Germany SANDRAWILSON • SophionBioscienceA/S,Ballerup,Denmark ZHONGJU XIAO • DepartmentofPhysiology,SouthernMedicalUniversity,Guangdong, Guangzhou,China EKATERINAZAITSEVA • IoneraTechnologiesGmbH,Freiburg,Germany YIZHOU • DepartmentofNeurobiology,SchoolofBasicMedicalSciences,Army Medical University,Chongqing,China
The list of books you might like
