Nucleic Acids and Protein Synthesis Part G

985 Pages·1979·22.26 MB·English

by  Kivie Moldave

Contributors to Volume LIX
Article numbers are in parentheses following the names of contributors.
snoitailiffA listed are current.
BERNADETTE L. ALFORD (5), Department ed Biologia Molecular, Facultad de Cien-
of Chemistry, Massachusetts Institute of cias, C.S.I.C. and Universidad Aut6noma
Technology. Cambridge, Massachusetts de Madrid, Canto Blanco, Madrid-34,
93120 Spain
RICARDO AMILS (38), Instituto de Bioqui- KATHRYN CALAME (60), Department of Bi-
mica de Macromol(culas, Centro de ology, California Institute of Technology,
Biologia Molecular, Facultad de Cien- Pasadena, California 52119
cias, Universidad Aut6noma ed Madrid, CHARLES R. CANTOR (38), Department of
Canto Blanco, Madrid-34, Spain Chemistry, Columbia University, New
IRENE L. ANDRULIS (20), Department of York, New York 72001
Medical Genetics, University of Toronto, LI-MING CHANGCHIEN (46), Laboratory of
Toronto, Ontario, Canada Molecular Biology, University of Wiscon-
STUART M. ARFIN (20), Department of Bi- sin-Madison, Madison, Wisconsin 60735
ological Chemistry, Collcoe of Medicine, A. CRAIG CHINAULT (5), Department of Bi-
University of California, lrvine, Califor- ological Sciences, University of Califor-
ain 71729 nia, Santa Barbara, California 60139
JUAN P. G. BALLESTA (58), lnstituto ed B. S. COOPERMAN (57), Department of
Bioquimica de Macromol~culas, Centro Chemistry, University of Pennsylvania,
ed Biologia Molecular, Facultad ed Cien- Philadelphia, Pennsylvania 40191
cias, Universidad AutOnoma de Madrid, FRIEDRICH CRAMER (9, 10, 22), Max-
Canto Blanco, Madrid-34, Spain Planck-lnstitut ri~J Experimentelle Medi-
V. I. BARANOV (30), Institute of Protein zin, Abteihmg Chemic, Hermann-Rein-
Research, Academy of Sciences of the Strasse ,3 D-3400 Ghttingen, Federal Re-
USSR, 292241 Poustchino, Moscow Re- public "~ Germany
gion, USSR GARY R. CRAVEN (45, 46, 47), Laboratory
W. EDGAR BARNETT (13, 14, 35), Graduate of Molecular Biology, University of Wis-
School of Biomedical Sciences, Univer- consin-Madison, Madison, Wisconsin
sity of Tennessee-Oak Ridge, and Biology 35 607
Division, Oak Ridge National Labora- ALBERT E. DAHLBERG (31), Division of -iB
tory, P.O. Box ,Y Oak Ridge, Tennessee ology and Medicine, Brown University,
03873 Providence, Rhode Island 21920
ROGERG DAMASCHUN (53), Central Institute
N. V. BELITSINA (30), Institute of Protein
of Molecular Biology, The Academy of
Research, Academy of Sciences of the
Sciences of the GDR, Department of
USSR, 292241 Poustchino, Moscow Re-
Small-Angle X-Ray Scattering, DDR-
gion, USSR
5111 Berlin-Buch, Federal Republic of
HEINZ BIELKA (53), Central Institute of
Germany
Molecular Biology, The Academy of Sci-
BERNARD D. DAVIS (28, 61), Bacterial Phys-
ences of the GDR, Department of Cell
iology Unit, Harvard Medical School,
Physiology, DDR-I I I5 Berlin-Buch, Fed-
Boston, Massachusetts 51120
eral Republic of Germany
JAN D1JK (40), Max-Planck-lnstitut fiir Mo-
MARK BIRENBAUM (59), Department of -iM lekulare Genetik, Abteilung Wittmann,
crobiology and Immunology, Washington lhnestrasse 63-73, D-IO00 Berlin 33 (Dah-
University School of Medicine, St. Louis, lem), Federal Republic of Germany
Missouri 01136 FERDINAND DOHME (37), AIthouse Labo-
BARTOEOMI~ CABRER (27), lnstituto de ratory, The Pennsylvania State Univer-
Bioquhnica de Macromol~culas, Centro sity, University Park, Pennsylvania 20861
xii
CONTRIBUTORS TO VOLUME L1X
BERNARD S. DUDOCK (12), Department of ROBERT GREENBERG (12), Department of
Biochemistry, State University of New Biochemistry, State University of New
York at Stony Brook, Stony Brook, New York at Stony Brook, Stony Brook, New
York 1 4971 York 11794
DAVID ELSON (39), Biochemistry Depart- CLAUDIO GUALERZI (56), Max-Planck-ln-
ment, The Weizmann Institute of Science, stitut fiir Molekulare Genetik, Abteilung
Rehovot, Israel Wittmann, lhnestrasse 63-73, D-IO00
DONALD M. ENGELMAN (49, 51), Depart- Berlin 33 (Dahlem), Federal Republic "1o
ment of Molecular Biophysics and Bio- Germany
chemistry, Yale University, New Haven, ARNOLD E. HAMPEL (16, 17), Department
Connecticut 06520 of Biological Sciences, Northern Illinois
M. DUANE ENGER (17), Cellular and Mo- University, DeKalb, Illinois 60115
lecular Biology Group, Los Alamos Sci- FRITZ HANSSKE (9, 10), Department of
entific Laboratories, Los Alamos, New Chemistry, The University of Alberta, Ed-
Mexico 87545 monton, Alberta, Canada T6G 2G2
STEPHEN R. FAHNESTOCK (36), Department G. WESLEY HATFIELD (15, 23, 25), Depart-
of Biochemistry and Biophysics, AIthouse ment of Medical Microbiology, College of
Laboratory, The Pennsylvania State Uni- Medicine, University of California, lr-
versity, University Park, Pennsylvania vine, California 92717
20861 SIDNEY M. HECHT (5), Department of
OL'GA O. FAVOROVA (18), Institute of Mo- Chemistry, Massachusetts Institute of
lecular Biology, Academy of Sciences of Technology, Cambridge, Massachusetts
the USSR, Vavilova str., 32, Moscow 02139
V-334, 117984 USSR LANNY I. HECKER (13, 14, 35), Chemical
THOMAS H. FRASER (6, 21), Research Lab- Carcinogenesis Group, Frederick Cancer
oratories, The Upjohn Company, Kala- Research Center, P.O. Box B, Frederick,
mazoo, Michigan 49001 Maryland 21701
G. FREYSSINET (35), Section Biologie Gen- EDGAR C. HENSHAW (33), Cancer Center
erale et Appliqude, Universit~ Lyon, 69 and Department of Medicine, University
Villeurbonne, Lyon, France of Rochester, Rochester, New York 14642
PHILIP T. GAFFNEY (47), Laboratory of
FRANCISCO HERNXNDEZ (58), lnstituto de
Molecular Biology, University of Wiscon-
Bioqulmica de Macromol~culas, Centro
sin-Madison, Madison, Wisconsin 53706
de Biolog(a Molecular, C.S.I.C. and
AMANDA M. GILLUM (3), Department of Universidad AutGnoma de Madrid, Canto
Pathology, Stanford University School of
Blanco, Madrid-34, Spain
Medicine, Palo Alto, California 94305
GABOR L. IGLIO (22), Max-Planek-lnstitut
TOMAS GIRBI~S (27), lnstituto de Bioquimica
fiir Experimentelle Medizin, Abteilung
de Macromoldculas, Centro de Biologla
Chemie. Hertnann-Rein-Strasse 3. D-
Molecular, Facultad de Ciencias, C.S.I.C.
3400 Gbttingen, Federal Republic of Ger-
and Universidad AutGnoma de Madrid,
many
Canto Blanco, Madrid-34, Spain
EMANUEL GOLDMAN (23, 25), Department GARRET M. IHLER (60), Department of
of Medical Microbiology, College of Medical Biochemistry, College of Medi-
Medicine, University of California, lr- cine, Texas A&M University, College
vine, California 92717 Station, Texas 77843
R. A. GOLDMAN (57), Department of Chem- SETSUKO O. JOLLY (5), Biological Labora-
istry, University of Pennsylvania, Phila- tories, Harvard University, Cambridge,
delphia, Pennsylvania 19104 Massachusetts 02139
P. G. GRANT (57), Department of Chemis- DAVID J. JULIUS (21), Department of Bio-
try, University of Pennsylvania, Philadel- chemistry, University of California,
phia, Pennsylvania 19104 Berkeley, California 94720
xiii
CONTRIBUTORS TO VOLUME LIX
JAMES W. KENNY (43), Department of -iB KIVIE MOLDAVE (32), Department of Bio-
ological Chemistry, School of Medicine, logical Chemistry, College of Medicine,
University of California, Davis, Califor- University of California, lrvine, -rofilaC
ain 61659 ain 71729
SUNG-Hou KIM (1), Department of Bio- PETER B. MOORE (49, 50), Department of
chemistry, Duke University Medical Cen- Chemistry and Molecular Biophysics and
ter, Durham, North Carolina 01772 Biochemistry, Yale University, New
LEV L. KISSELEV (18), Institute of Molec- Haven, Connecticut 02560
ular Biology, Academy of Sciences of the JORGEN J. MOLLER (53), Central Institute
USSR, Vavilova str., ,23 Moscow, ,433-V of Molecular Biology, ehT Academy of
489711 USSR Sciences of the GDR, Department of
M. H. J. KOCH (52), European Molecular Small-Angle X-Ray Scattering, DDR-
Biology Laboratory, Notkestrasse ,58 5111 Berlin-Buch, Federal Republic of
2000 Hamburg ,25 Federal Republic of Germany
Germany YO-ICHI NABESHIMA (42), Department of
V. E. YKSNAILETOK (48), Institute of Pro- Biochemistry, Niigata University School
tein Research, Academy of Sciences of of Medicine, Niigata, Japan
the USSR, 292241 Poustchino, Moscow DAI NAKADA (60), Department of Biochem-
Region, USSR istry, University of Pittsburgh School of
GALINA K. KOVALEVA (18), Institute of Medicine, Pittsburgh, Pennsylvania 16251
Molecular Biology, Academy of Sciences A. W. NICHOLSON (57), Department of
of the USSR, Vavilova str. ,23 Moscow Chemistry, University of Pennsylvania,
,433-V 489711 USSR Philadelphia, Pennsylvania 40191
JOHN M. LAMBERT (43), Department of -iB
KNUD H. NIERHAUS (37, 55, 62), Max-
ological Chemistry, School of Medicine,
Planck-hlstitut fiir Molekulare Genetik,
University of California, Davis, Califor-
Abteilung Wittmann, lhnestrasse 63-73,
nia 61659
00OI-D Berlin 33 (Dahlem), Federal Re-
ALAN M. LAMBOWITZ (34), ehT Edward A.
"cilbup of Germany
Daisy Department of Biochemistry, St.
IALOK1N VEALOK1N (59), Institute of Bio-
Louis University Medical School, St.
chemisto', Bulgarian Academy of Sci-
Louis, Missouri 40136
ence, Sofia, Bulgaria
JENNY LITTLECHILD (40), Max-Planck-In-
stitut fiir Molekulare Genetik, Abteilung KIKUO ATA~tO (41, 42), Department of Bio-
Wittmann, lhnestrasse ,37-36 00OI-D Ber- chemistry, Niigata University School of
nil 33 (Dahlem), Federal Republic of -reG Medicine, Niigata, Japan
many YELNATS M. SNOSRAP (25), Department of
M. A. LUDDY (57), Department of Chem- Chemistry, University of CaliJbrnia,
istry, University of Pennsylvania, Phila- Santa Barbara, Calibrnia 60139
delphia, Pennsylvania 40191
AIHTNYC L. NOP (56), Max-Planck-lnstitut
ELIZABETH A. MATTHEWS (38), Depart-
flit Molekulare Genetik, Abteilung Witt-
ment of Chemistry, Columbia University,
mann, lhnestrasse 63-73, D-IO00 Berlin
New York, New York 72001
33 (Dahlem), Federal "cilbupeR of Ger-
A. MINNELLA (57), Department of Chem-
many
istry, University of Pennsylvania, Phila-
GWEN PRIOR (24), Department of Medicine
delphia, Pennsylvania 40191
and ehT Franklin McLean Memorial Re-
JUAN MODOLELL (27), lnstituto de Bioqul-
search Institute, ehT University of Chi-
mica de Macromoldculas, Centro de
cago, Chicago, Illinois 60637
Biologla Molecular, Facultad de Cien-
cias, C.S.I.C. and Universidad Aut6noma YRAG J. QUIGLEY (1), Department of -loiB
ed Madrid, Canto Blanco, Madrid-34, ogy, Massachusetts Institute of Technol-
Spain ogy, Cambridge, Massachusetts 93120
xiv
CONTRIBUTORS TO VOLUME LIX
JESSE C. RABINOWITZ (29), Department of 280, D-6900 Heidelberg, Federal Repub-
Biochemistry, University of California, cil of Germany
Berkeley, California 02749 DAVID SCSLESSINGER (59), Department of
MURRAY RABINOWITZ (24), Department of Microbiology and Immunology, Washing-
Medicine and The Franklin McLean ton University School of Medicine, St.
Memorial Research Institute, ehT Uni- Louis, Missouri 01136
versity of Chicago, Chicago, Illinois HUBERT J. P. SCHOEMAKER (26), Corning
73606 Medical Center, Medfield, Massachusetts
UTTAM L. RAJBHANDARY (3), Department 25020
of Biology, Massachusetts Institute of LADONNE H. SCHULMAN (7, 8), Depart-
Technology, Cambridge, Massachusetts ment of Developmental Biology and Can-
93120 cer, Albert Einstein College of Medicine,
BRIAN R. REID (2), Department of Bio- Bronx, New York 16401
chemistry, University of CaliJornia, Riv- S. D. SCHWARTZSACH (13, 14, 35), School
erside, CaliJornia 12529 of Life Sciences, University of Nebraska,
SCOTT A. REINES (7), Department of Psy- Lincoln, Nebraska 80586
chiatry, Montefiore Hospital and Medical JEAN SCHWARZBAUER (45), Laboratory of
Center, Bronx, New York 76401 Molecular Biology, University of Wiscon-
ALEXANDER RICH (6, 21), Department of sin-Madison, Madison, Wisconsin 60735
Biology, Massachusetts Institute of Tech- F. SEELA (9), Universitiit Paderborn-Ges-
nology, Cambridge, Massachusetts 93120 amthochschule, Lab. Jiir Bioorganische
PRESTON O. RITTER (17), Department of Chemie. Warburger Strasse 100, 0974-D
Chemistry, Eastern Washington Univer- Paderborn, Federal Republic of Germany
sity, Cheney, Washington 40099 IGOR N. SERDYUK (54), Institute of Protein
BRUCE RUEFER (16), Department of Biolog- Research, Academy of Sciences of the
ical Sciences, Northern Illinois Univer- USSR, 292241 Poustchino, Moscow Re-
sity, DeKalb, Illinois 51106 gion, USSR
WAYNE SAaO (32), Department of Biologi- WILLIAM J. SHARROCK (29), Medical Re-
search Council, Laboratory of Molecular
cal Chemistry, College of Medicine, Uni-
Biology, Cambridge 2BC 2QH, England
versity of California, Irvine, California
VICTOR SHEN (59), Department of Micro-
71729
biology and Immunology, Washington
ISAAC SADNIK (32), Department of Biolog-
University School of Medicine, St. Louis,
ical Chemistry, College of Medicine,
Missouri 01136
University of California, lrvine, Califor-
MELVIN SILBERKLANG (3), Department of
ain 71729
Biochemistry and Biophysics, University
ASOK K. SARKAR (8), Department of De-
of California, San Francisco, California
velopmental Biology and Cancer, Albert
34149
Einstein College of Medicine, Bronx,
A. S. SPIRIN (30), Institute of Protein Re-
New York 16401
search, Academy of Sciences of the
JEROME A. SCHIFE (35), Institute of Pho- USSR, 142292 Poustchino, Moscow Re-
tobiology of Cells and Organelles, Bran- gion, USSR
deis University, Waltham, Massachu- PNINA SPITNIK-ELSON (39), Biochemistry
setts 45120
Department, The Weizmann Institute of
PAUL R. SCHIMMEE (26), Department of Science, Rehovot, Israel
Biology, Massachusetts Institute of Tech- MATHIAS SPRINZL (11), Mux-Planck-lnsti-
nology, Cambridge, Massachusetts 93120 tut fiir Experimentelle Medizin, Abteilung
HANS-GEORG SCHLEICH (4), lnstitut fiir Chemie, Hermann-Rein-Strasse ,3 -D
Zellforschung am Deutschen Krebsfor- 0043 .negnitt~fG Federal Republic of -reG
schungszentrum lm Neuenheimer Feld many
CONTRIBUTORS TO VOLUME LIX XV
HANS STERNBACH (11), Max-Planck-lnstitut USSR, 292241 Poustchino, Moscow Re-
fi~r Experimentelle Medizin, Abteilung gion, USSR
Chemie, Hermann-Rein-Strasse ,3 0043-D FRIEDRICH NOV RED HAAR (19, 22), Max-
,negnitt~iG Federal Republic of Germany Planck-lnstitut fiir Experintentelle Medi-
W. A. STRYCHARZ (57), Institute for En- zin, Ahteihmg Chemie, Hermunn-Rein
zyme Research, University of Wisconsin, Strasse ,3 D-3400 G6ttingen, Federal Re-
Madison, Wisconsin 50735 public qf Germany
H. B. STUHRMANN (52), European Molec- KIMITSUNA WATANARE (9), Mitsubishi-
ular Biology Laboratory, Notkestrasse Kasei Institute of Life Sciences, Mina-
,58 2000 Hamburg ,25 Federal Republic miooya H, Machida-Shi, Tokyo, Japan
of Germany WOLFGANG WINTERMEYER (4), lnstitut fiir
PrlANG-C. TAI (28, 61), Bacterial Physiol- Physiologische Chemie, Physikalische
ogy Unit, Harvard Medical School, Bos- Biochemie und Zellbiologie der Univer-
ton, Massachusetts 51120 sitiit Miinchen, Goethestrasse ,33 0008-D
KAZUO TERAO (41, 42), Department of Bio- Miinchen ,2 Federal Republic of Germany
chemistry, Niigata University School of GABRIELE M. WVSTUP (55), Friedrich-
Medicine, Niigata, Japan Miescher Institut, Basel, Switzerland
HIROSHI TERAOKA (62), Shionogi Research H. G. ZACHAU (4), Institut fiir Physiolo-
Laboratory, Fukushima-ku, Osaka 533, gische Chemie, Physikalische Biochemie
Japan und Zellbiologie red Universitiit Miinchen,
ROBERT R. TRAUT (43), Department of -iB Goethestrasse ,33 D-8000 Miinchen ,2
ological Chemistry, School of Medicine, Federal Republic of Germany
University of California, Davis, Califor- RADOVAN ZAK (24), Department of Medi-
ain 61659 cine and The Franklin McLean Memorial
KUNIO TSURUGI (42), Department of Bio- Research Institute, The University of
chemistry, Niigata University School of Chicago, Chicago, Illinois 73606
Medicine, Niigata, Japan ROBERT A. ZIMMERMANN (44), Department
V. D. VASILIEV (48), Institute of Protein of Biochemistry, University of Massa-
Research, Academy of Sciences of the chusetts, Amherst, Massachusetts 30010
Preface
Since the publication of the last two volumes of "Nucleic Acids and
Protein Synthesis" (Volume XXIX, Part E and Volume XXX, Part F),
numerous novel, revealing, and sophisticated techniques have been de-
veloped. Some of these techniques are reflected in the articles that are
included in the current two volumes, LIX, Part G and LX, Part H.
Volume LIX deals mainly with transfer RNA, the aminoacyl-tRNA
synthetases, and ribosomes. Methods for tRNA preparation, fractiona-
tion, structure determination, modification, sequencing, aminoacylation,
and utilization in protein synthesis are detailed in the first part of this
Volume. The latter part presents methods for the preparation of ribonu-
cleoprotein particles from a variety of sources, chemical and enzymatic
modification, physical-chemical analyses, fractionation, and reconstruc-
tion. Volume LX deals with the isolation, purification, regulation, and
determination of protein factors that are involved in translation in a
variety of prokaryotic and eukaryotic cells. Much information has already
been provided by these techniques, and we hope that they will continue
to do so for users of these volumes.
We wish to thank our many colleagues who have so generously con-
tributed of their time and effort and the staff of Academic Press for their
valuable assistance.
KIVIE EVADLOM
ECNERWAL NAMSSORG
xvii
METHODS IN ENZYMOLOGY
DETIDE YB
Sidney P. Colowick and Nathan O. Kaplan
VANDERRILT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDICINE UNIVERSITY OF CALIFORNIA
NASHVILLE~ TENNESSEE AT SAN DIEGO
LA JOLLA~ CALIFORNIA
I. Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
III. Preparation and Assay of Substrates
IV. Special Techniques for the Enzymologist
V. Preparation and Assay of Enzymes
VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
VII. Cumulative Subject Index
xix
METHODS IN ENZYMOLOGY
FEIHC-NI-SROTIDE
Sidney P. Colowick Nathan O. Kaplan
EMULOV VIII. Complex Carbohydrates
Edited yb ELIZABETH F. NEUFELD DNA ROTCIV GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited by WILLIS A. DOOW
EMULOV X. Oxidation and Phosphorylation
Edited by DLANOR W. KOORBATSE DNA DRANYAM E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited yb C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited yb J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited yb J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited yb DNOMYAR B. NOTYALC
EMULOV XWI. Fast Reactions
Edited yb KENNETH N!TSUK
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited yb TREBREH ROBAT DNA CELIA WHITE ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited yb DLANOD B. KCIMROCCM DNA LEMUEL D. THGIRW
EMULOV XIX. Proteolytic Enzymes
Edited yb EDURTREG E. NNAMLREP DNA LASZLO DNAROL
ixx
xxii
METHODS IN ENZYMOLOGY
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part )D
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited yb WILLIAM B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part )A
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited yb ROTCIV GRUBSNIG
EMULOV XXlX. Nucleic Acids and Protein Synthesis (Part E)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited yb YENDIS REHCSIELF DNA LESTER REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited yb SIDNEY REHCSIELF DNA LESTER REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited yb AHTRAM G. DENNIS DNA DRAWDE A. SINNED
METHODS IN ENZYMOLOGY XXlll
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited yb WILLIAM B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part B)
Edited yb JOHN M. NIETSNEWOL
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited yb JOEL G. NAMDRAH DNA BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and
Organ Systems)
Edited yb JOEL G. NAMDRAH DNA BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part )B
Edited by W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part )C
Edited yb W. A. DOOW
EMULOV XLIII. Antibiotics
Edited yb JOHN H. HASH
EMULOV XLIV. Immobilized Enzymes
Edited yb KLAUS HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by LASZLO DNAROL
EMULOV XLVI. Affinity Labeling
Edited by WILLIAM B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XLVII. Enzyme Structure (Part E)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT