Normal and Malignant Cell Growth PDF
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Normal and Malignant Cell Growth Recent Results in Cancer Research Fortschritte der Krebsforschung Progres dans les recherches sur Ie cancer 17 Edited by V. G. Allfrey, New York· M. Allgower, Chur . K. H. Bauer, Heidelberg· 1. Beren blum, Rehovoth . F. Bergel, London . J. Bernard, Paris . W. Bernhard, Villejuif N. N. Blokhin, Moskva· H. E. Bock, Tubingen . P. Bucalossi, Milano· A. V. Chaklin, Moskva . M. Chorazy, Gliwice . G. J. Cunningham, London . W. Dameshek, Boston M. Dargent, Lyon . G. Della Porta, Milano . P. Denoix, Villejuif . R. Dulbecco, San Diego· H. Eagle, New York· R. Eker, Oslo· P. Grabar, Paris· H. Hamperl, Bonn R. J. C. Harris, London· E. Hecker, Heidelberg· R. Herbeuval, Nancy· J. Higgin son, Lyon· W. C. Hueper, Bethesda· H. Isliker, Lausanne· D. A. Karnofsky, New York· J. Kieler, K¢benhavn • G. Klein, Stockholm . H. Koprowski, Philadelphia o. L. G. Koss, New York· G. Martz, Zurich· G. Mathe, Villejuif . Muhlbock, Am sterdam . G. T. Pack, New York· V. R. Potter, Madison· A. B. Sabin, Cincinnati L. Sachs, Rehovoth . E. A. Saxen, Helsinki . W. Szybalski, Madison . H. T agnon, Bruxelles . R. M. Taylor, Toronto . A. Tissieres, Geneve . E. Uehlinger, Zurich R. W. Wissler, Chicago· T. Yoshida, Tokyo· L. A. Zilber, Moskva Editor in chief P. Rentchnick, Geneve Springer-Verlag New York, Inc. 1969 Normal and Malignant Cell Growth Edited by J. R. M. Fry, M. L. Griem and W. H. Kirsten With 85 Figures Springer-Verlag New York, Inc. 1969 Symposium on Normal and Malignant Cell Growth The University of Chicago School of Medicine February 24th and 25th, 1968 Sponsored by the Swiss League agaimt Cancer ISBN-l3: 978-3-642-48265-6 e-ISBN-13: 978-3-642-48263-2 DOl: 10.1007/978-3-642-48263-2 All righ ts reserved. No part of this book may be translated or reproduced in any form without written permission from Springer-Verlag. © 1969 by Springer-Verlag New York Inc. Softcover reprint of the hardcover 1s t edition 1969 Library of Congress Card Number 65-6383 Title No. 3632 Contents Preface ix Introduction xi List of Participants Xlll Normal Cell Kinetics Chairman: M. FRY General Introduction: L. LAMERTON . 1 Proliferative Capacity of Hemopoietic Stem Cells: L. LAJTHA 10 Discussion: Colony Forming Units in the Bone Marrow of Partial Body Irradiated Mice: V. P. BOND and A. L. CARSTEN 21 Cellular Proliferation in the Liver: J. W. GRISHAM 28 Discussion: Cellular Proliferation in the Liver: NANCY L. BUCHER, F. L. MOOLTEN and T. R. SCHROCK 43 Cell Proliferation in the Intestinal Epithelium: S. LESHER and J. BAUMAN 49 Discussion: Proliferation of Gastrointestinal Cells: M. LIPKIN 56 General Discussion 60 Normal Cell Kinetics (cont.) Chairman: L. LAMERTON Proliferation of Antibody Forming Cells: D. W. TALMADGE 66 Discussion: Proliferation of Antibody Forming Cells: D. A. ROWLEY, F. W. FITCH and D. E. MOISER 75 Composition of Cell Life Cycle: D. M. PRESCOTT 79 Discussion: Methods and Criteria of Mammalian Cell Synchrony: W. K. SINCLAIR . 90 Susceptibility of Intestinal Crypt Epithelium to Hydroxyurea and Other Antitumor Agents: F. S. PHILIPS, S. S. STERNBERG and L. LENAZ 104 vii viii CONTENTS Discussion: Mammalian Cell Killing by Inhibitors of DNA Synthesis: G. F. WHITMORE, J. BORSA, S. BACHETTI and F. GRAHAM . 109 Proliferation of Cancer Cells Chairman: A. M. BRUES General Introduction: The Growth of Tumor Cells under Normal and Fasting Conditions: R. BASERGA and F. WIEBEL 118 Cell Proliferation during Carcinogenesis: A. B. REISKIN 128 Comparison Between the Rates of Proliferation of Induced Malignancies and Their Normal Tissues of Origin: F. D. BERTALANFFY . 136 The Use of Three-Dimensional Matrix Tissue Culture for Bioassay of Cancer: A Progress Report: J. LEIGHTON, G. JUSTH and R. MARK . 147 Discussion: The Use of Three-Dimensional Matrix Tissue Culture for Bioassay of Cancer: K. SANFORD . 163 Importance of the Non-Dividing Leukemia Cell: A. M. MAUER, E. F. SAUNDERS and B. C. LAMPKIN 168 Discussion: Magnitude of Proliferating Fraction and Rate of Proliferation of Populations of Leukemic Cells in Man: B. D. CLARKSON, J. FRIED and M. OGAWA 175 Incorporation of Iodine-Labeled 5- Iodo-2'- Deoxyuridine into DNA of Mouse Mammary Tumors: L. A. DETHLEFSEN 186 In Vivo Cell Kinetics of Human Cancers: M. TUBIANA 202 Summary: H. M. PATT . 218 Round-Table Discussion 226 Preface This volume presents the Proceedings of the University of Chicago's third Cancer Training Grant supported Teaching Symposium. This Symposium received much of its support from grant number T12 CA 08077-02. Most of the planning of the Symposium and most of the local editing of the Proceedings was carried out by Dr. R. J. Michael Fry of the A.E.C. Argonne National Laboratory and by two members of the Advisory Committee of the Cancer Training Grant, Drs. Melvin Griem and Werner Kirsten. They carried the main responsibility for the Symposium. The subject of the Symposium, "Normal and Malignant Cell Growth," was chosen because, as the Proceedings reflect, it is a rapidly advancing field of endeavor which is of utmost importance to the understanding of the processes of malignant neoplasia. In fact, there is increasing evidence that knowledge of the kinetics of the cancer cell will greatly influence approaches to cancer therapy. Like the first two of these Teaching Symposia held in 1964 and 1966, this one attracted about 400 students and staff from this medical institution as well as from other medical centers in the Chicago area. The effective interplay of an excellent group of scientists with a lively and responsive audience was evident as they considered together a topic of great current interest in the field of neoplasia. Much of the credit for the smooth organization and implementation of the Symposium must go to Mrs. Brenda K. Pickens who was ably assisted by Mrs. Jean B. Newgard. Mrs. Pickens' contribution to recording, transcribing and editing the Proceedings is also gratefully acknow ledged. As director of the Cancer Training Grant, I also want to express my appreciation for the contributions of the Advisory Committee to this program and specifically to the success of this Symposium. They are as follows: GEORGE E. BLOCK, Professor of Surgery and Coordinator of Education in Clinical Oncology. MELVIN L. GRIEM, Associate Professor of Radiology in the A.E.C. Argonne Cancer Research Hospital and Director, Registry of Neoplastic Diseases. ELWOOD V. JENSEN, American Cancer Society-Charles Hayden Foundation Research Professor of Physiology in the Ben May Laboratory for Cancer Research. JOSEPH B. KIRSNER, Professor of Medicine and Chief of Gastroenterology. WERNER H. KIRSTEN, Professor of Pathology and Pediatrics and N.C.I. Career De velopment Awardee. JOHN V. PROHASKA, Professor of Surgery. HENRY RAPPAPORT, Professor of Pathology and Director of Surgical Pathology. ROBERT W. WISSLER, Ph.D., M.D., Director of Cancer Training Grant Professor and Chairman Department of Pathology University of Chicago Pritzker School of Medicine and in the A.E.C. Argonne Cancer Research Hospital Introduction The understanding of the proliferation of cells and tissues has proceeded at a rapid pace over the last decade. The one particularly significant accomplishment has been the introduction of labelled DNA precursors and the use of high resolution auto radiography. These techniques have allowed the physiologist and the pathologist to examine many additional parameters in the process of cell proliferation both in vitro and in vivo. Knowledge of cell proliferation may be useful in improving our methods of treatment of a number of diseases and particularly a number of the human cancers which defy current treatment methods. The Cancer Training Grant Director at The University of Chicago, with the help of his Advisory Committee, made plans to hold a teaching symposium on the subject of normal and malignant cell growth as part of the annual program of train ing in oncology at The University of Chicago. The objectives of the conference were to survey the recent accomplishments in this field and to present for the medical students and house staff the newer developments in the cell proliferation kinetics of normal tissues. As with the previous symposia sponsored by this training grant, lead ing scientists in various aspects of cell proliferation were invited to present general reviews as well as their latest results. In a number of instances the speaker was fol lowed by an invited discussant. The first day of the symposium, February 24, 1968, was devoted to the under standing of cell proliferation kinetics and was devoted to the general topics related to normal cell proliferation. A discussion followed this first day symposium so that the main speakers could exchange additional information and could answer questions from the audience. This discussion was especially stimulating under the leadership of Dr. Hewson Swift. On February 25, 1968, the participants considered our current knowledge of the proliferation of tumors. The symposium was summarized most ably by Dr. Harvey Patt. The form of abbreviation for tritiated thymidine still shows the individuality of authors, but as their meaning is clear, no changes have been made. In order to prevent further reduction of figures, some have been placed to make optimal use of page size. It is felt that the reader will use the table of contents for his guidance and we hope therefore will not miss the presence of an index. This symposium held at The University of Chicago has stimulated a number of the staff and the graduate and undergraduate students to organize a weekly confer ence devoted to the discussion of problems related to cell kinetics. The symposium was supported in part by the Rosa Kuhn Levy Fund, The Chicago Tumor Institute, the Rockefeller Foundation Grant for the Activities in Nuclear Medicine (Pharmacology), and mainly by the Cancer Training Grant of the National Cancer Institute #T12 CA 08077-02. Weare grateful to all who have made this conference possible. R. J. M. FRY, M. 1. GRIEM, and W. H. KIRSTEN List of Participants BASERGA, RENATO GRISHAM, J. W. Fels Research Institute and Department of Pathology Department of Pathology Washington University Temple University St. Louis, Missouri Philadelphia, Pennsylvania LAJTHA, LASZLO G. BERTALANFFY, FELIX D. Patterson Research Laboratories Department of Anatomy Christie Hospital and Holt Radium University of Manitoba Institute Winnipeg, Canada Manchester, England BOND, VICTOR P. LAMERTON, LEONARD F. Medical Department Department of Biophysics Brookhaven National Laboratory Institute of Cancer Research Upton, New York Surrey, England BRUES, AUSTIN M. Division of Biological and LEIGHTON, JOSEPH Medical Research Department of Pathology Argonne National Laboratory and University of Pittsburgh The University of Chicago Pittsburgh, Pennsylvania Argonne, Illinois LESHER, SAMUEL W. BUCHER, NANCY L. R. Department of Radiology John Collings Warren Laboratories Allegheny General Hospital Huntington Memorial Hospital Pittsburgh, Pennsylvania Harvard University Boston, Massachusetts LIPKIN, MARTIN Department of Medicine CLARKSON, BAYARD Cornell University Sloan-Kettering Institute for New York, New York Cancer Research New York, New York MAUER, ALVIN M. Children's Hospital Research DETHLEFSEN, LYLE A. Foundation and Department of Radiology Department of Pediatrics University of Pennsylvania University of Cincinnati Philadelphia, Pennsylvania Cincinnati, Ohio J. FRY, R. MICHAEL Division of Biological and PATT, HARVEY M. Medical Research Laboratory of Radiobiology Argonne National Laboratory University of California Argonne, Illinois San Francisco, California xiii xiv LIST OF PARTICIPANTS PHILIPS, FREDERICK S. SINCLAIR, WARREN K. Pharmacology Division Division of Biological and Sloan-Kettering Institute Medical Research for Cancer Research Argonne National Laboratory New York, New York Argonne, Illinois PRESCOTT, DAVID SWIFT, HEWSON H. Institute for Developmental Biology Department of Biology University of Colorado University of Chicago Boulder, Colorado Chicago, Illinois REISKIN, ALLAN B. Division of Biological and Medical TALMAGE, DAVID W. Research Departments of Microbiology and Argonne National Laboratory and Medicine The University of Chicago University of Colorado Argonne, Illinois Denver, Colorado ROWLEY, DONALD A. TUBIANA, MAURICE Department of Pathology Department of Radiation University of Chicago Institute Gustave-Roussy Chicago, Illinois Villejuif, France SANFORD, KATHERINE K. Laboratory of Biology WHITMORE, GORDON National Cancer Institute University of Toronto and National Institutes of Health Ontario Cancer Institute Bethesda, Maryland Toronto, Canada
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