Table Of ContentMUSCLE, MATRIX, AND
BLADDER FUNCTION
ADV ANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
Editorial Board:
NATHAN BACK, State University of New York at Buffalo
IRUN R. COHEN, The Weizmann Institute of Science
DA VID KRITCHEVSKY, Wistar Institute
ABEL LAJTHA, N. S. Kline Institute for Psychiatric Research
RODOLFO PAOLETTI, University of Milan
Recent Volumes in this Series
Volume 379
SUBTILISIN ENZYMES: Practical Protein Engineering
Edited by Richard Bott and Christian Betzel
Volume 380
CORONA-AND RELATED VIRUSES: Current Concepts in Molecular Biology
and Pathogenesis
Edited by Pierre J. Talbot and Gary A. Levy
Volume 381
CONTROL OF THE CARDIOVASCULAR AND RESPIRATORY SYSTEMS IN
HEALTH AND DISEASE
Edited by C. Tissa Kappagoda and Marc P. Kaufman
Volume 382
MOLECULAR AND SUBCELLULAR CARDIOLOGY: Effects of Structure and Function
Edited by Samuel Sideman and Rafael Beyar
Volume 383
IMMUNOBIOLOGY OF PROTEINS AND PEPTIDES VIII: Manipulation or Modulation
of the Immune Response
Edited by M. Zouhair Atassi and Garvin S. Bixler, Jr.
Volume 384
FATIGUE: Neural and Muscular Mechanisms
Edited by Simon C. Gandevia, Roger M. Enoka, Alan J. McComas, Douglas G. Stuart,
and Christine K. Thomas
Volume 385
MUSCLE, MATRIX, AND BLADDER FUNCTION
Edited by Stephen A. Zderic
Volume 386
INHIBITORS TO COAGULATION FACTORS
Edited by Louis M. Aledort, Leon W. Hoyer, Jeanne M. Lusher, Howard M. Reisner,
and Gilbert C. White III
Volume 387
BIOLOGICAL REACTIVE INTERMEDIATES V: Basic Mechanistic Research in
Toxicology and Human Risk Assessment
Edited by Robert Snyder, I. Glenn Sipes, George F. Kalf, David J. Jollow,
Helmut Greim, James J. Kocsis, Terrence J. Monks, and Charlotte M. Witmer
A Continuation Order Plan is available for this series. A continuation order wil1 bring delivery of each new volume
immediately upon publication. Volumes are bil1ed only upon actual shipment. For further information please contact
the publisher.
MUSCLE, MATRIX, AND
BLADDER FUNCTION
Edited by
Stephen A. Zderic
University of Pennsylvania
School of Medicine
Children's Hospital of Philadelphia
Philadelphia, Pennsylvania
SPRINGER SCIENCE+BUSINESS MEDIA, LLC
Library of Congress Cataloging-in-Publication Data
On file
Proceedings of a symposium on Muscle, Matrix, and Bladder Function,
held March 18-19, 1994, in Philadelphia, Pennsylvania
ISBN 978-1-4899-1587-0 ISBN 978-1-4899-1585-6 (eBook)
DOI 10.1007/978-1-4899-1585-6
© Springer Science+Business Media New York 1995
Originally published by Plenum Press, New York in 1995
Softcover reprint of the hardcover 1st edition 1995
1098765432 1
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any
means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written
permission from the Publisher
PREFACE
This conference and monograph were the result of many collective efforts. The whole
concept was formulated one early Wednesday morning at our weekly research meeting at
Children's Hospital in our division of urology. We have been most fortunate to have a close
collaboration with Bob Levin, Ed Macarak, and Pam Howard who have helped steer the
course of our division's growing interest in basic science. At our weekly meetings our
laboratory fellow will summarize their current work. Other ongoing areas of investigation in
our labs and elsewhere are discussed. We have always made an effort to try and understand
what other groups are doing who are working in the area of bladder smooth muscle research.
It occurred to us that the best way to really know what everyone working in this field was
doing would be to sponsor a 2-day meeting where we could all gather to discuss our ongoing
work. A major limitation of the annual meeting of the American Urologic Association or the
urology section of the American Academy of Pediatrics is that the scientfic sessions are limited
as these are meant to be primarily clinical meetings (as they should be). For this reason the
idea of a meeting devoted solely to research about the urinary bladder had great appeal. In
addition to allowing for longer presentations than the standard 5 to 7 minutes, every effort
would be made to encourage a dialogue amongst the presenters and the audience. We were
pleased that so many people took their time to come to Philadelphia, and participate.
No editor should ever fail to acknowledge the many assistants without whom such a
meeting and book could be completed. It all started with Olga Korytko fromour Office of
Medical Education at Children's who tended to the details of putting on such a meeting. The
administration of this effort was made so much easier thanks to her experience and know
how. My office staff in the division of urology fielded endless phone calls and last minute
requests, and over the course of the past two years have handled countless changes in
scheduling and other last minute requests. To Marolyn Buchanan I extend special thanks for
her assistance with the pesky details associated with the preparation of a camera-ready
manuscript. At Plenum Publishing Eileen Bcrmingham and Robert Wheeler provided
valuable guidance in steering this project to completion. A listing of our corporate and NIH
sponsors appears on the next page and I am grateful to them for the interest they have shown.
No project like this ever gets completed without a major boost from one's family and I
wish to thank my wife Kate (who has the stamina to direct a busy department of pediatric
emergency medicine and organize our home), and our children, Olivia and Colin, for all they
have given me. Kate and I wish to acknowledge her parents Joan and Charles Cronan, who
always seem to magically appear when our schedules are on a collision course. They have all
tolerated the endless piles of manuscripts, late hours, and occasional lapses of memory that
often pulled me away physically and mentally, at times when I was especially needed. I am
forever grateful for their inspiration, love, and support.
Stephen A. Zderic MD
v
ACKNOWLEDGMENTS
Support from the following organizations is gratefully acknowledged, for without this
funding, the meeting and this book would not have been possible.
The National Institutes of Health
Conference Grant R-13 DK DK47242
Smith Kline Beecham Pharmaceuticals
Sandoz Pharmaceuticals
Glaxo Research Institute
Ortho McNeil Pharmaceuticals
Pfizer Research PLC
C.R. Bard Inc.
vi
CONTENTS
The Clinical Relevance of Basic Science: A Surgeon's Perspective 1
John W. Duckett
Bladder Outlet Obstruction - Overview 3
AlanJ. Wein
Bladder Function in Experimental Outlet Obstruction: Pharmacologic 7
Responses to Alterations in Innervation, Energetics, Calcium
Mobilization, and Genetics.
Robert M. Levin, Niels Haugaard, Sheila S. Levin, Ralph Buttyan,
Min-Wei Chen, Frederick C. Monson, and Alan J. Wein
Electrical Properties of Obstructed Guinea Pig Bladder 21
Jacek L Mostwin, Narihito Seki, Orner Karim, and Gommert van Koeveringe
Metabolism of Detrusor Smooth Muscle in Normal and Obstructed 29
Urinary Bladder
Bengt Uvelius and Anders Amer
The Neuronal Response to Bladder Outlet Obstruction, a Role for NGF 41
Mary C. DuPont, Katarina Persson, John Spitsbergen, Jeremy B. Tuttle,
and W.D. Steers
Contractile Proteins and Their Response to Bladder Outlet Obstruction 55
Samuel Chacko and Penelope A. Longhurst
Molecular Aspects of Bladder Outlet Obstruction 65
Victor K. Lin and John D. McConnell
Bladder Outlet Obstruction - Discussion 75
Developmental Physiology - Overview 81
William C. De Groat
Fetal Lower Urinary Tract Physiology In Vivo Studies 85
Robert Mevorach and Barry Kogan
Fetal Bovine Compliance Changes 93
Laurence S. Baskin, Dave Meaney, Adam Landsman, Serban
Constantinescu, and Edward Macarak
Developmental Aspects of Excitation Contraction Coupling in
Urinary Bladder Smooth Muscle 105
Stephen A. Zderic, Chaoliang Gong, Joseph Hypolite,
and Robert M. Levin
vii
Congential Bladder Obstruction: Research Strategies and Directions 117
Craig A. Peters
Developmental Bladder Physiology -Discussion 131
Extracellular Matrix Update -Overview 141
Gary Striker
Is Collagen Remodeling Associated with Bladder Obstruction? 143
H. Paul Enrlich
Regulation of Matrix Metalloproteinases during Extracellular
Matrix Turnover 1 51
Marta L. Corcoran, David E. Kleiner, Jr. and William G. Stetler-Stevenson
Elastic Fibers and Their Role in Bladder Extracellular Matrix 161
Joel Rosenbloom, Harry Koo, Pamela S. Howard, Robert Mecham,
and Edwatd J. Macatak
The Collagens and Their Urologic Implications 173
Edwatd 1. Macatak, David Ewalt, Laurence Baskin, Douglas Coplen, Harry
Koo, Robert Levin, John W. Duckett, Howatd Snyder, Joel Rosenbloom,
and Pamela S. Howatd
Extracellular Matrix Update - Discussion 179
Changes in Bladder Extracellular Matrix - Overview 185
Timothy Ratliff
Matrix Changes in the Bladder Associated With Normal Aging 187
Ellen Shapiro and Herbert Lepor
The Role of Proteoglycans in Bladder Structure and Function 191
Bertram J. Levy and Thomas N. Wight
Cultured Mechanical Bladder Cells and Their Response to
Mechanical Strain 207
Douglas E. Coplen, Pamela S. Howatd, John W. Duckett,
Howard M. Snyder, and Edwatd J. Macatak
Alterations in Extracellular Matrix Gene Expression in Normal
Versus Non-Compliant Human Bladders 215
Pamela S. Howatd, David H. Ewalt, John W. Duckett, Howatd M. Snyder,
and Edward 1. Macatak
Changes in Bladder Extracellular Matrix - Discussion 223
Regenerative Bladder Augmentation: A Review of the Initial .
Preclinical Studies With Porcine Small Intestinal Submucosa 229
Bradley P. Kropp, Stephen Badylak, and Katl B. Thor
The Effects of High Urinary Potassium Concentration of Pelvic
Nerve Mechanoreceptors and "Silent" Afferents From the
Rat Bladder 237
Jiang Wen and J. F. B Morrison
Muscarinic Receptor Subtypes in Normal, Fetal, and Gravid Rabbit
Bladder, Heart, and Uterus 241
Steven B. Brandes and Michael R. Ruggieri
viii
Fetal Bovine Bladder Physiology and Pharmacology 251
Harry P. Koo, Edward J. Macarak., John W. Duckett, Howard M. Snyder,
Alan J. Wein, and Robert M. Levin
Two Mathematical Models Predict the Variation in Capacity and
Compliance of Hypertrophied Bladders 255
Margot S. Damaser and Steven L. Lehman
The Effects of Cholinergic Stimulation on Cultured Smooth Muscle
Cells 257
S. Bruce Malkowicz, Juanita Thomas, and Robert M. Levin
Autologous Periurethral Fat Injections for the Creation of Bladder
Outlet Obstruction 261
Douglas A. Canning
Participants and Faculty 265
Index 269
ix
