Table Of Content

Mrutyunjay Suar Namrata Misra Chandravanu Dash Editors Microbial Engineering for Therapeutics Microbial Engineering for Therapeutics (cid:129) (cid:129) Mrutyunjay Suar Namrata Misra Chandravanu Dash Editors Microbial Engineering for Therapeutics Editors MrutyunjaySuar NamrataMisra KIITSchoolofBiotechnology TechnologyBusinessIncubator KIITUniversity KIITUniversity Bhubaneswar,Odisha,India Bhubaneswar,Odisha,India ChandravanuDash Blvd,Hubbard MeharryMedicalCollege Nashville,TN,USA ISBN978-981-19-3978-5 ISBN978-981-19-3979-2 (eBook) https://doi.org/10.1007/978-981-19-3979-2 #TheEditor(s)(ifapplicable)andTheAuthor(s),underexclusivelicensetoSpringerNatureSingapore PteLtd.2022 Thisworkissubjecttocopyright.AllrightsaresolelyandexclusivelylicensedbythePublisher,whether thewholeorpartofthematerialisconcerned,specificallytherightsoftranslation,reprinting,reuseof illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similarordissimilarmethodologynowknownorhereafterdeveloped. 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The registered company address is: 152 Beach Road, #21-01/04 Gateway East, Singapore 189721, Singapore Contents PartI IntroductiontoMicrobialEngineering 1 ExploringthePotentialofMicrobialEngineering: TheProspect,Promise,andEssence. . . . . . . . . . . . . . . . . . . . . . . . 3 SaumyaDarshanaPatra,AryashreeArunima,andMrutyunjaySuar PartII MicrobialEngineeringApproaches 2 RoleofSystemBiologyinMicrobialSystem. . . . . . . . . . . . . . . . . . 43 SriyaPriyadarshani,ParitoshPatel,andSushreeSahoo 3 SyntheticBiology:RefiningHumanHealth. . . . . . . . . . . . . . . . . . . 57 ShaileshKumarSamalandSubhamPreetam 4 GutMicrobiomeandObesity:ConnectingLink. . . . . . . . . . . . . . . 71 JayshreeMishra,KhyatiAmin,LongxiangKuang, andNarendraKumar 5 EngineeringMicrobesforSmartDiagnosticsandLab-on-Chip. . . . 101 SwatiSrijaya,ParitoshPatel,SureshK.Verma, andYogendraKumarMishra 6 BacteriophageandVirusEngineering. . . . . . . . . . . . . . . . . . . . . . . 123 SubratKumar,R.Pattnaik,P.S.Sahu,andB.Bhattacharya 7 CRISPRTechnologies:AToolforEngineeringMicrobes. . . . . . . . 145 SangeetaJaiswal PartIII HealthBenefits 8 RecombinantVaccines:TheRevolutionAhead. . . . . . . . . . . . . . . . 163 AbhijeetNayak 9 Microbe–HostMetabolicInteraction:ProbioticApproach. . . . . . . 201 K.R.KarthikaParvathy,BibekanandMallick,YuwaleeUnpaprom, GaantyPrakashManiam,NatanamurugarajGovindan, andParamasivanBalasubramanian vv vi Contents 10 DesignerMicrobes:OncotherapyApproach. . . . . . . . . . . . . . . . . . 231 JugalKishoreDasandAryashreeArunima 11 TheHumanGutMicrobiomeinHealth,Disease, andTherapeutics. . . . . .. . . . .. . . . .. . . . .. . . . . .. . . . .. . . . .. . 249 NiladriBhusanPati,SwarupaPanda,andFrodeLarsJahnsen 12 UseofEngineeredBacteriafortheProductionofGreen ChemicalandPharmaceuticals. . . . . . . . . . . . . . . . . . . . . . . . . . . . 261 ChitranRoyChowdhury,VenkataS.Sabbisetti, andAmrendraK.Ajay 13 FatFightingMicrobes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 293 RanjitKumarMehta PartIV InnovationandTranslation 14 Scale-UpofEngineeringStrainforIndustrialApplications. . . . . . . 311 LopamudraRayandVishakhaRaina 15 AffordableTherapeuticsThroughEngineeredMicrobes. . . . . . . . . 327 SusritaSahooandNamrataMisra 16 COVID-19:LessonLearntfromDiagnosticstoTherapeutics. . . . . 345 PritamKumarPanda,VivekP.Chavda,KunalNeve,SumanMishra, SureshK.Verma,andRajeevAhuja Editors and Contributors About the Editors MrutyunjaySuar istheDirectorGeneralofR&DandInnovationatKIITUniver- sity, Bhubaneswar, and the CEO of KIIT Technology Business Incubator (KIIT- TBI).HeisanentrepreneurialprofessorwithaPhDinMolecularMicrobiologyfrom the University of Delhi in the year 2003 and trained as a postdoc at ETH Zurich, Switzerland(Feb2004–June2007).HereturnedtoIndiaandestablishedtheSchool ofBiotechnologyin2007underKIITUniversitywhichbecameaCentreofExcel- lenceawardedbytheDepartmentofBiotechnology,GovernmentofIndia.Healso established KIIT-TBI, promoting deep tech start-up companies. Under his leader- ship,KIIT-TBInurturedmorethan300+start-upcompaniesinthelast12yearsand created5000+jobsandestablishedtheCentreofExcellenceinincubationinthearea ofDigitalHealth,Diagnostics&Precisionagriculture,supportedbytheDepartment of Science & Technology, Govt of India. He is a passionate mentor and has been deeplyinvolvedinmentoringdeep-techstart-upcompaniesandhelpedtotakethem through their journey from ideation to enterprise creation. He received several recognitions including Samanta Chandrasekhar Award-2019 from Odisha Bigyan Academy,AmericanSocietyofMicrobiology(ASM)Professorship awardin2012 and visited UCLA, USA, GP Chatterjee Memorial award during 102nd Indian Science Congress, and Future young technical leaders by STARS Switzerland in 2010. He was also nominated forGlobal EntrepreneurshipLeadership Symposium atHAASBusinessSchool,USA,andvisitedUCBerkley. NamrataMisra ispresentlytheHead-TechnologyIncubationatKIITTechnology Business Incubator, Bhubaneswar, which is one of the leading incubators in the country and has incubated more than 250 companies so far. She holds a Master’s degreefromBanarasHinduUniversityandaPhDinLifeSciencefromAcademyof Scientific and Innovative Research, ACSIR (CSIR-IMMT), Government of India. ShehasreceivedPost-DoctoralFellowshipunderScienceandEngineeringResearch Board, Government of India. At KIIT-TBI, she isinvolvedin coordination, imple- mentation,andmonitoringofvariousinnovativeprogramsofministrieslikeBIRAC, DBT, DST, Meity, Startup Odisha, Startup India, and Invest India for technology developmentfromideationtocommercialization.DrMisrahasalsobeenacatalyst vviiii viii EditorsandContributors inaugmentinginnovationecosystembybeinganactivememberoftheTechnology Enabling Centre at KIIT University, supported by DST and the BIRAC Regional Centre at KIIT-TBI focusing on East and North-East states of India and Bhubaneswar City Knowledge Innovation Cluster (BCKIC), supported by PSA, Government of India. Besides being Head-Technology Incubation at the KIIT- TBI, Dr Misra is Assistant Professor in the Department of Biotechnology, KIIT University. Her research and teaching interests includes study of vital metabolic pathways and in-depth sequence-structural analysis of underpinning enzymes to decipher the molecular mechanism of important biological processes in host. She has authored or coauthored more than 45 peer-reviewed international journal publications. She has also developed several cutting-edge computations tools, databases, and web servers which have been widely used by scientific commu- nity. Recently, she has been honored with the Odisha Young Scientist Award by theDepartmentofScience&Technology,GovernmentofOdisha. ChandravanuDash PhDisanAssociateProfessorattheCenterforAIDSHealth Disparities Research (CAHDR) and the Department of Biochemistry and Cancer BiologyattheMeharryMedicalCollege,Nashville,TN,USA.Heisabiochemistin training andhasbeen studyingviruses formore than two decades.He receivedhis PhD in biochemistry from the National Chemical Laboratory in India and post- doctoral training at the National Cancer Institute HIV Drug Resistance Program (NCI-NIH).HejoinedMeharrywithanNIHK99/R00granttostarthisindependent researchprogramfocusingonthemechanismofretroviralreplicationandpathogen- esis.HisresearchhasbeencontinuouslyfundedbyNIHandotherfundingagencies. Hehasauthoredmorethan50peer-reviewedpublicationsandregularlyservesasa peerreviewerformanyjournalsandNIHgrantproposals.Heservesasamemberof the TN-CFAR executive committee and also served as the co-director of the NIH-funded Tennessee Center for AIDS Research (TN-CFAR) Personized Care ScientificWorkingGroup(PC-SWG).Inaddition,hewasamemberoftheInternal Advisory Committees of the Research Centers in Minority Institutions (RCMI) at MeharryandtheMeharryTranslationalResearchCenter(MeTRC).Recently,hehas beenappointedtoserveastheco-directoroftheMeharry2026StrategicPlanningfor ResearchandamemberoftheMeharryResearchAdvisoryCommittee. Contributors Rajeev Ahuja Condensed Matter Theory Group, Materials Theory Division, DepartmentofPhysicsandAstronomy,UppsalaUniversity,Uppsala,Sweden Department of Physics, Indian Institute of Technology Ropar, Rupnagar, Punjab, India Amrendra K. Ajay Division of Renal Medicine, Department of Medicine, BrighamandWomen’sHospital,Boston,MA,USA DepartmentofMedicine,HarvardMedicalSchool,Boston,MA,USA EditorsandContributors ix KheyatiAmin DepartmentofPharmaceuticalSciences,ILRCollegeofPharmacy TexasA&MHealthScienceCenter,Kingsville,TX,USA Aryashree Arunima Department of Microbial Pathogenesis and Immunology, TexasA&MUniversityHealthScienceCenter,Bryan,TX,USA ParamasivanBalasubramanian DepartmentofBiotechnologyandMedicalEngi- neering,NationalInstituteofTechnologyRourkela,Rourkela,Odisha,India B. Bhattacharya School of Biotechnology, KIIT Deemed to be University, Bhubaneswar,India Vivek P. Chavda Department of Pharmaceutics and Pharmaceutical Technology, L.M.CollegeofPharmacy,Ahmedabad,India Chitran Roy Chowdhury Division of Renal Medicine, Department of Medicine, BrighamandWomen’sHospital,Boston,MA,USA Jugal Kishore Das Department of Microbial Pathogenesis and Immunology, TexasA&MUniversityHealthScienceCenter,Bryan,TX,USA Natanamurugaraj Govindan Faculty of Industrial Sciences and Technology, UniversitiMalaysiaPahang,Kuantan,Pahang,Malaysia Frode Lars Jahnsen Institute of Clinical Medicine, University of Oslo, Oslo, Norway SangeetaJaiswal DivisionofGastroenterology,DepartmentofInternalMedicine, UniversityofMichigan,AnnArbor,MI,USA LongxiangKuang DepartmentofPharmaceuticalSciences,ILRCollegeofPhar- macyTexasA&MHealthScienceCenter,Kingsville,TX,USA Narendra Kumar Department of Pharmaceutical Sciences, ILR College of Phar- macyTexasA&MHealthScienceCenter,Kingsville,TX,USA Subrat Kumar School of Biotechnology, KIIT Deemed to be University, Bhubaneswar,India Bibekanand Mallick Department of Life Science, National Institute of Technol- ogyRourkela,Rourkela,Odisha,India Gaanty Prakash Maniam Faculty of Industrial Sciences and Technology, UniversitiMalaysiaPahang,Kuantan,Pahang,Malaysia Ranjit Kumar Mehta Department of Radiation Oncology, University of Michigan,AnnArbor,MI,USA Jayshree Mishra Department of Pharmaceutical Sciences, ILR College of Phar- macyTexasA&MHealthScienceCenter,Kingsville,TX,USA SumanMishra SchoolofBiotechnology,KIITUniversity,Bhubaneswar,India x EditorsandContributors Yogendra Kumar Mishra Mads Clausen Institute, NanoSYD, University of SouthernDenmark,Sønderborg,Denmark NamrataMisra SchoolofBiotechnology,KalingaInstituteofIndustrialTechnol- ogy(KIIT)DeemedtobeUniversity,Bhubaneswar,India KIIT-Technology Business Incubator (KIIT-TBI), Kalinga Institute of Industrial Technology(KIIT)DeemedtobeUniversity,Bhubaneswar,India Abhijeet Nayak Department of Infectious Diseases, Center for Experimental and MolecularMedicine,Amsterdam,TheNetherlands KunalNeve PharmacySection,L.M.CollegeofPharmacy,Ahmedabad,India Pritam Kumar Panda Condensed Matter Theory Group, Materials Theory Divi- sion,DepartmentofPhysicsandAstronomy,UppsalaUniversity,Uppsala,Sweden SwarupaPanda InstituteofClinicalMedicine,UniversityofOslo,Oslo,Norway K.R.KarthikaParvathy DepartmentofLifeScience,NationalInstituteofTech- nologyRourkela,Rourkela,Odisha,India ParitoshPatel SchoolofBiotechnology,KIITUniversity,Bhubaneswar,India Niladri Bhusan Pati Institute of Clinical Medicine, University of Oslo, Oslo, Norway Saumya Darshana Patra School of Biotechnology, KIIT University, Bhubaneswar,India R. Pattnaik School of Biotechnology, KIIT Deemed to be University, Bhubaneswar,India SubhamPreetam InstituteofAdvancedMaterials,IAAM,Ulrika,Sweden CentreforBiotechnology,SikshaOAnusandhanUniversity,Bhubaneswar,India Sriya Priyadarshani School of Biotechnology, KIIT University, Bhubaneswar, India Vishakha Raina School of Biotechnology, KIIT Deemed to be University, Bhubaneswar,Odisha,India Lopamudra Ray School of Biotechnology, KIIT Deemed to be University, Bhubaneswar,Odisha,India SchoolofLaw,KIITDeemedtobeUniversity,Bhubaneswar,Odisha,India Venkata S. Sabbisetti Division of Renal Medicine, Department of Medicine, BrighamandWomen’sHospital,Boston,MA,USA DepartmentofMedicine,HarvardMedicalSchool,Boston,MA,USA SushreeSahoo DepartmentofHaematology,St.JudeChildren’sResearchHospi- tal,Memphis,TN,USA