Methods Used in Adenosine Research PDF
Preview Methods Used in Adenosine Research
METHODS IN PHARMACOLOGY Volume 6 Methods Used in Adenosine Research General Editor: Arnold Schwartz Baylor Col/ege of Medicine, Houston, Texas Volume 1 Edited by Arnold Schwartz Volume 2: PHYSICAL METHODS Edited by Colin F. Chignell Volume 3: SMOOTH MUSCLE Edited by Edwin E. Daniel and David M. Paton Volume 4A:RENAL PHARMACOLOGY Edited by Manuel Martinez·Maldonado Volume 4B: RENAL PHARMACOLOGY Edited by Manuel Martinez-Maldonado Volume 5: MYOCARDIAL BIOLOGY Edited by Arnold Schwartz Volume 6: METHODS USED IN ADENOSINE RESEARCH Edited by David M. Paton A Continuation Order Plan is available for this series. A continuation order will bring delivery of each new volume immediately upon publication. Volumes are billed only upon actual shipment. For further information please contact the publisher. METHODS IN PHARMACOLOGY Volume 6 Methods Used in Adenosine Research Edited by David M. Paton University of Auckland Auckland, New Zealand PLENUM PRESS • NEW YORK AND LONDON Library of Congress Cataloging in Publication Data Main entry under title: Methods used in adenosine research. (Methods in pharmacology; v. 6) Includes bibliographies and index. I. Adenosine. 2. Adenosine-Research-Methodology. I. Paton, David M. (David Murray), 1938- . II. Series. [DNLM: I. Adenosine. 2. Research-methods. WI ME9616N v.6 / QU 58 M5928] QP905.M45 vol. 6 61Y.1s [615'.71] 84-26638 [QP625.A27] ISBN 978-1-4684-4888-7 ISBN 978-1-4684-4886-3 (eBook) DOI 10.1007/978-1-4684-4886-3 © 1985 Plenum Press, New York Softcover reprint of the hardcover 1s t edition 1985 A Division of Plenum Publishing Corporation 233 Spring Street, New York, N.Y. 10013 All rights reserved No part of this book may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher To my wife Beth, our children Heather and Fiona, and my father Don Contributors RAM P. AGARWAL CAROL E. CASS Section of Medical Oncology Cancer Research Group, McEachern Evans Memorial Department of Clinical Laboratory Research University of Alberta Departments of Medicine and Pharmacology Edmonton, Alberta, Canada and Hubert H. Humphrey Cancer Research Center Boston University Medical Center PETER K. CHIANG Boston, Massachusetts Division of Biochemistry Walter Reed Army Institute of Research Washington, D.C. ROBERT M. BERNE Department of Physiology NOEL J. CUSACK University of Virginia School of Medicine Department of Pharmacology Charlottesville, Viriginia King's College London London, England NOEL J. BUCKLEY ADRIAAN DEN HERTOG Department of Anatomy and Embryology Department of Pharmacology Center for Neuroscience State University University College, London Groningen, The Netherlands London, England JEFFREY S. FEDAN Physiology Section GEOFFREY BURNSTOCK National Institute for Occupational Safety and Department of Anatomy and Embryology Health Center for Neuroscience Department of Pharmacology and Toxicology University College, London West Virginia University Medical Center London, England Morgantown, West Virginia vii viii Contributors BERTIL B. FREDHOLM University of South Florida College of Department of Pharmacology Medicine Karolinska Institute Tampa, Florida Stockholm, Sweden H. J. LEIGHTON Department of Pharmacology ERIC R. HARLEY The Wellcome Research Laboratories Cancer Research Group, McEachern Burroughs Wellcome Company Laboratory Research Triangle Park, North Carolina University of Alberta Edmonton, Alberta, Canada JOHN P. O'DONNELL School of Pharmacy J. FRANK HENDERSON West Virginia University Medical Center Cancer Research Group, McEachern Morgantown, West Virginia Laboratory and Department of Biochemistry University of Alberta R. A. OLSSON Edmonton, Alberta, Canada Suncoast AHA Chapter Cardiovascular Research Laboratory Departments of Internal Medicine and Biochemistry G. KURT HOGABOOM University of South Florida College of Department of Pharmacology and Toxicology Medicine West Virginia University Medical Center Tampa, Florida Morgantown, West Virginia ROBERT E. PARKS, JR. LOWIE P. JAGER Section of Biochemical Pharmacology Department of Pharmacology Division of Biology and Medicine Central Veterinary Institute Brown University Lelystad, The Netherlands Providence, Rhode Island ALAN R. P. PATERSON SIMON M. JARVIS Cancer Research Group, McEachern Department of Physiology Laboratory University of Alberta University of Alberta Edmonton, Alberta, Canada Edmonton, Alberta, Canada TERRY P. KENAKIN DAVID M. PATON Department of Pharmacology Department of Pharmacology and Clinical The Wellcome Research Laboratories Pharmacology Burroughs Well come Company University of Auckland Research Triangle Park, North Carolina Auckland, New Zealand S. KUSACHI J. D. PEARSON Suncoast AHA Chapter Cardiovascular Section of Vascular Biology Research Laboratory MRC Clinical Research Centre Department of Internal Medicine Harrow, Middlesex, England Contributors ix ULRICH SCHWABE DIANNE R. WEBSTER Pharmakologisches Institut Department of Pharmacology and Clinical der Universitat Heidelberg Pharmacology Heidelberg, Federal Republic of Germany University of Auckland School of Medicine Auckland, New Zealand JOHANNA D. STOECKLER Section of Biochemical Pharmacology Division of Biology and Medicine DAVID P. WESTFALL Brown University Department of Pharmacology Providence, Rhode Island University of Nevada School of Medicine Reno, Nevada T. W. STONE Department of Physiology St. George's Hospital Medical School THOMAS D. WHITE University of London Department of Pharmacology London, England Dalhousie University Halifax, Nova Scotia, Canada R. D. THOMPSON Suncoast AHA Chapter Cardiovascular Research Laboratory JAMES D. YOUNG Department of Internal Medicine Department of Biochemistry University of South Florida College of Faculty of Medicine Medicine The Chinese University of Hong Kong Tampa, Florida Shatin, N.T., Hong Kong Preface In their classic paper in 1929, Drury and Szent-Gyorgyi described a number of the important cardiovascular actions of adenosine. Another thirty years were to pass before the possible physiological role of adenosine in coronary vasodilation was studied by Berne and others. Since then, there has been a tremendous increase in research into the actions of adenosine. Workers from many disciplines have employed a wide variety of techniques, since adenosine is a product of and a substrate for a number of metabolic pathways, is transported into cells, and acts at discrete receptor sites to modulate the activity of adenylate cyclase and to produce important actions on many cells and tissues including platelets, adipo cytes, heart, blood vessels, and other smooth muscles. International symposia on the actions of adenosine were held in 1978, 1981, and 1982, and the proceedings of these symposia have been published (Baer and Drummond, 1979; Daly et at., 1983; Berne et at., 1983). Since it is not the primary purpose of the present volume to review our current understanding of the nu merous actions of adenosine, these volumes should be consulted for such details. Rather, the present volume has been planned to provide both graduate students and investigators in pharmacology and related disciplines with a summary of some of the methods now available for the study of the actions of adenosine and, in particular, to highlight their possible uses and limitations. The volume has been organized into sections dealing with related topics. It is hoped that this format will facilitate use of the volume and that it may introduce readers to techniques they have not used before or provide deeper insights into their use. Space has not allowed coverage to be given to all the methods or topics that might have been included, such as the effects of adenosine on platelet function and on immunological processes. Such omissions are certainly not intended to imply that these actions are unimportant. As the editor, I am pleased to acknowledge the tremendous support I have received from the contributors to this volume. They come from eight nations and xi xii Preface are among the foremost investigators in this research area. Without their expertise and cooperation, this volume would not have been possible. I personally have found the editing of the volume an educational and rewarding experience, and it is hoped that readers will similarly benefit from its use. The editing of this volume would not have been possible without the careful and thorough assistance I have received from my secretary, Mrs. Brenda Carlson. Her help is gratefully acknowledged, as is that of my colleague, Dr. Dianne R. Webster, and of my other secretarial assistants in Auckland (Mrs. J. Simpson and Mrs. J. Williamson) and Cape Town (Miss F. Clarke). David M. Paton Auckland, New Zealand REFERENCES Baer, H. P., and Drummond, G. I. 1979. Physiological and Regulatory Functions of Adenosine and Adenine Nucleotides. Raven Press, New York. Berne, R. M., Rail, T. W., and Rubio, R. 1983. Regulatory Functions ofA denosine. Martinus Nijhoff, The Hague. Daly, J. W., Kuroda, Y., Phillis, J. W., Shimizu, H., and Ui, M. 1983. Physiology and Pharmacology of Adenosine Derivatives. Raven Press, New York. Drury, A. N., and Szent-Gyorgyi, A. 1929. The physiological activity of adenine compounds with especial reference to their action upon the mammalian heart. J. Physiol. (Lond)., 68:213-237.
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