Mechanics of Biological Systems and Materials, Volume 6: Proceedings of the 2016 Annual Conference on Experimental and Applied Mechanics PDF
Preview Mechanics of Biological Systems and Materials, Volume 6: Proceedings of the 2016 Annual Conference on Experimental and Applied Mechanics
Conference Proceedings of the Society for Experimental Mechanics Series Chad S. Korach · Srinivasan Arjun Tekalur Pablo Zavattieri Editors Mechanics of Biological Systems and Materials, Volume 6 Proceedings of the 2016 Annual Conference on Experimental and Applied Mechanics Conference Proceedings of the Society for Experimental Mechanics Series Series Editor Kristin B. Zimmerman, Ph.D. Society for Experimental Mechanics, Inc. Bethel,CT,USA More information about this series at http://www.springer.com/series/8922 Chad S. Korach (cid:129) Srinivasan Arjun Tekalur (cid:129) Pablo Zavattieri Editors Mechanics of Biological Systems and Materials, Volume 6 Proceedings of the 2016 Annual Conference on Experimental and Applied Mechanics Editors ChadS.Korach SrinivasanArjunTekalur DepartmentofMechanicalEngineering MichiganStateUniversity StateUniversityofNewYork EastLansing,MI,USA atStonyBrook StonyBrook,NY,USA PabloZavattieri PurdueUniversity WestLafayette,IN,USA ISSN2191-5644 ISSN2191-5652 (electronic) ConferenceProceedingsoftheSocietyforExperimentalMechanicsSeries ISBN978-3-319-41350-1 ISBN978-3-319-41351-8 (eBook) DOI10.1007/978-3-319-41351-8 LibraryofCongressControlNumber:2016949413 #TheSocietyforExperimentalMechanics,Inc.2017 Thisworkissubjecttocopyright.AllrightsarereservedbythePublisher,whetherthewholeorpartofthematerialisconcerned,specificallytherightsof translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission orinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdeveloped. Theuseofgeneraldescriptivenames,registerednames,trademarks,servicemarks,etc.inthispublicationdoesnotimply,evenintheabsenceofaspecific statement,thatsuchnamesareexemptfromtherelevantprotectivelawsandregulationsandthereforefreeforgeneraluse. Thepublisher,theauthorsandtheeditorsaresafetoassumethattheadviceandinformationinthisbookarebelievedtobetrueandaccurateatthedateof publication.Neitherthepublishernortheauthorsortheeditorsgiveawarranty,expressorimplied,withrespecttothematerialcontainedhereinorforany errorsoromissionsthatmayhavebeenmade. Printedonacid-freepaper ThisSpringerimprintispublishedbySpringerNature TheregisteredcompanyisSpringerInternationalPublishingAGSwitzerland Preface MechanicsofBiologicalSystemsandMaterialsrepresentsoneoftenvolumesoftechnicalpaperspresentedattheSEM2016 Annual Conference and Exposition on Experimental and Applied Mechanics organized by the Society for Experimental MechanicsandheldinOrlando,FL, June6–9,2016.ThecompleteproceedingsalsoincludesvolumesonDynamicBehavior of Materials; Challenges in Mechanics of Time-Dependent Materials; Advancement of Optical Methods in Experimental Mechanics; Experimental and Applied Mechanics; Micro-and Nanomechanics; Mechanics of Composite and Multifunc- tional Materials; Fracture, Fatigue, Failure and Damage Evolution; Residual Stress, Thermomechanics and Infrared Imaging,HybridTechniquesandInverseProblems;andJoiningTechnologiesforCompositesandDissimilarMaterials. Eachcollectionpresentsearlyfindingsfromexperimentalandcomputationalinvestigationsonanimportantareawithin experimentalmechanics,themechanicsofbiologicalsystemsandmaterialsbeingoneoftheseareas. Thisvolumesummarizestheexchangeofideasandinformationamongscientistsandengineersinvolvedintheresearch and analysis of how mechanical loads interact with the structure, properties, and function of living organisms and their tissues. The scope includes experimental, imaging, numerical, and mathematical techniques and tools spanning various lengths and time scales. Establishing this symposium at the Annual Meeting of the Society for Experimental Mechanics providesavenuewherestate-of-the-artexperimentalmethodscanbeleveragedinthestudyofbiomechanics.Amajorgoal of the symposium was for participants to collaborate in the asking of fundamental questions and the development of new techniquestoaddressbio-inspiredproblemsinsociety,humanhealth,andthenaturalworld.Theorganizerswouldliketo thankallthespeakersandstaffatSEMforenablingasuccessfulprogram. StonyBrook,NY,USA ChadS.Korach EastLansing,MI,USA SrinivasanArjunTekalur WestLafayette,IN,USA PabloZavattieri v Contents 1 MechanicAdaptabilityofMetastaticCellsinColonCancer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 V.Palmieri,D.Lucchetti,M.Papi,F.Calapa`,G.Ciasca,A.Sgambato,andM.DeSpirito 2 Nano-MechanicalResponseofRedBloodCells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 MassimilianoPapi,GabrieleCiasca,ValentinaPalmieri,GiuseppeMaulucci, CristinaRossi,EleonoraMinelli,andMarcoDeSpirito 3 ScaleDependenceoftheMechanicalPropertiesofInterfacesinCrustaceansThinFilms. . . . . . . . . . . 17 DevendraVerma,TaoQu,andVikasTomar 4 DynamicAnalysisofHumanKnee. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 S.Yoshida,U.Tiwari,A.Saladino,M.Nguyen,D.Hollander,B.Boudreaux,andB.Hadley 5 ViscohyperelasticCalibrationinMechanicalCharacterizationofSoftMatter. . . . . . . . . . . . . . . . . . . 33 E.Ficarella,L.Lamberti,M.Papi,M.DeSpirito,andC.Pappalettere 6 ContactZoneEvaluationofDentalImplantsUsingDigitalPhotoelasticity. . . . . . . . . . . . . . . . . . . . . . 39 M.P.HariprasadandK.Ramesh 7 EvolutionoftheSkinMicrostructuralOrganizationDuringaMechanicalAssay. . . . . . . . . . . . . . . . . 45 B.Lynch,S.Bancelin,C.Bonod-Bidaud,F.Ruggiero,M.-C.Schanne-Klein,andJ.-M.Allain 8 ANumericalStudyofaBiaxialSollicitationtoSet-UptheDisplacement FieldMeasurementofExVivoMouseSkin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 J.-S.Affagard,F.Wijanto,R.RubioAmador,C.Bonod-Bidaud,F.Ruggiero,andJ.-M.Allain 9 DynamicPolarizationMicroscopyforInSituMeasurementsofCollagenFiber RealignmentDuringImpact. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 XianyuWu,Hsiao-YingShadowHuang,MarkPankow,andKaraPeters 10 Self-ShiftingNeutralAxisandNegativePoisson’sRatioinHierarchicalStructured NaturalComposites:Bamboo. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 ShaowenXu,AniruddhaMitra,StephenMigues,JacobMayfield,MichaelShinall, BessenbacherDerek,DavisLinley,andSpratlinRussell 11 High-SpeedHolographyforIn-VivoMeasurementofAcousticallyInducedMotions ofMammalianTympanicMembrane. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 PayamRazavi,JeffreyTaoCheng,CosmeFurlong,andJohnJ.Rosowski 12 RheologyofSoftandRigidMicroParticlesinCurvedMicrofluidicChannels. . . . . . . . . . . . . . . . . . . 83 JiaLiu,YuhaoQiang,MichaelMian,WeiheXu,andE.Du 13 MicrofluidicApproachesforBiomechanicsofRedBloodCells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 E.Du 14 CustomIndentationSystemforMechanicalCharacterizationofSoftMatter. . . . . . . . . . . . . . . . . . . . 95 ChelseySimmons,AndresRubiano,DanielStewart,andBrandeyAndersen vii viii Contents 15 ExperimentalEvaluationofBlastLoadingsontheEarandHeadwithandWithout HearingProtectionDevices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 TimJ.Walilko,RyanD.Lowe,TedF.Argo,G.DougMeegan,NathanielT.Greene, andDanielJ.Tollin 16 AMechano-HydraulicModelofIntracranialPressureDynamics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 D.Evans,C.Drapaca,andJ.P.Cusumano 17 RegionalVariationsintheMechanicalStrainsoftheHumanOpticNerveHead. . . . . . . . . . . . . . . . . 119 DanE.Midgett,MaryE.Pease,HarryA.Quigley,MohakPatel,ChristianFranck, andThaoD.Nguyen 18 ExperimentalElectromechanicsofRedBloodCellsUsingDielectrophoresis-Based Microfluidics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129 YuhaoQiang,JiaLiu,MichaelMian,andE.Du 19 MicrobucklingofFibrousMatricesEnablesLongRangeCellMechanosensing. . . . . . . . . . . . . . . . . . 135 BrianBurkel,AyeletLesman,PhoebusRosakis,DavidA.Tirrell, GuruswamiRavichandran,andJacobNotbohm 20 TheGrowthandMechanicalPropertiesofAbaloneNacreMesolayer. . . . . . . . . . . . . . . . . . . . . . . . . 143 AnqiZhang,YanChen,MariAnneSullivan,andBartonC.Prorok 21 EvaluationofPreciseOptimalCyclicStrainforTenogenicDifferentiationofMSCs. . . . . . . . . . . . . . 149 YasuyukiMorita,ToshihiroSato,SachiWatanabe,andYangJu 22 EffectofFiberArchitectureontheCellFunctionsofElectrospunFiberMembranes. . . . . . . . . . . . . . 157 F.Sultana,M.Vaughan,andM.Khandaker 23 ControllinghESC-CMCellMorphologyonPatternedSubstratesOveraRangeofStiffness. . . . . . . . 161 BrettN.Napiwocki,MaxR.Salick,RandolphS.Ashton,andWendyC.Crone 24 CytoskeletalPerturbingDrugsandTheirEffectonCellElasticity. . . . . . . . . . . . . . . . . . . . . . . . . . . . 169 MarthaE.Grady,RussellJ.Composto,andDavidM.Eckmann Chapter 1 Mechanic Adaptability of Metastatic Cells in Colon Cancer V.Palmieri,D.Lucchetti,M.Papi,F.Calapa`,G.Ciasca,A.Sgambato,andM.DeSpirito Abstract Tumourmicroenvironmentcontributesimportantlytothephenotypedevelopmentofcancercells,whichreceive andadapttochemicalandphysicalsignals.Itishypothesizedthatremodellingoftheactincytoskeletonmayenabletumor cellstoevadenormalapoptoticsignallingandpermittheacquisitionofmetastaticpropertiessuchasanchorage-independent growthandenhancedcellmigration.Amongdifferentcancercelllines,theSW480andSW620coloncells,derivedfromthe same patient at different stages of tumour progression, represent a unique model to study the changes in mechanical propertiesduringmetastases.TheSW480derivefromaprimarycolontumour,whiletheSW620originatefromapopulation ofmalignantcellsappearedinalymphnodemetastasesmonthsaftertheinitialsurgery.InthisworkwemeasuredbyAFM, combinedwithElectronandConfocalMicroscopy,themechanicadaptabilityofcancercellwhichreachedinvivothelymph node environment. We demonstrate how to resist to apoptotic stimuli in the lymphatic circulation, where the flow shear stress reduce cell survival, the metastatic cell increase their non-specific adhesion while retaining a flexible and soft cytoskeleton,relatedtouncontrolledgrowthproperties.Thesefeaturesallowaccomplishingthespreadinginthecirculation aimofmetastaticcells. Keywords Cancermechanics•Colon•AFM•SEM 1.1 Introduction Cancermechanicsisthestudyofforcesinvolvedintheintricateinterplaybetweencellsandextracellularenvironment.The emerging idea in this research field is that forces acting on cells can control biochemical signals responsible for cell proliferation,migrationandapoptosis[1].Cellssenseandrespondtothemechanicalpropertiesoftheenvironment,suchas the stiffness of the extracellular matrix (ECM) orthe compression exerted by neighbouringtissues, by balancing external forceswithchangesincytoskeletonorganizationandshaperemodelling.Thisresponseleadstotheactivationofsignalling pathwaysofcellsspreading,growth,motilityanddeath[2]. The understanding of the forces that link environment and tumour cells can help to understand the metastatic process, leadingcauseofmortalityamongcancerpatients.Duringthisprocess,cellsdetachfromneighboursoftheprimarytumor, remodel the external matrix and migrate towards the vasculature and lymphatics. These processes require dynamic modulationofcellshapeandcytoskeletontogetherwithchangesingeneexpression[2].Forexamplemetastaticcellscan detachbyalteringsurfaceproteinsexpressionandreducingadhesionorbyalteringcytoskeletonpolymerizationinorderto acquireahigherplasticitytosqueezeeasilythroughECM[1]. Specificcellularandextra-cellularmechanicalpropertieshaverecentlybeenexploitedaspossiblebiomarkersofdisease [3].Inparticular,incancerprogression,mechanicalpropertieswouldallowidentifyingphysicalattributesofcellsthatare morelikelytometastasize[3,4]. Inthisstudywecomparedthemechanicalandmorphologicalfeaturesofcoloncancercell populationsisolatedfroma primarytumorandfromitslymph-nodemetastases.WeusedtheSW480andSW620coloncancercelllinesthatofferthe unique advantage of having been isolated from the same patient at different stages of cancer progression. In particular SW480celllineoriginatesfromaDukes’stageBcoloncarcinomawhiletheSW620celllinehasbeenderivedfromalymph V.Palmieri(*)•M.Papi•G.Ciasca•M.DeSpirito InstituteofPhysics,Universita`CattolicadelSacroCuore,L.goFrancescoVito1,00168Rome,Italy e-mail:[email protected] D.Lucchetti(*)•F.Calapa`•A.Sgambato InstituteofPathology,Universita`CattolicadelSacroCuore,L.goFrancescoVito1,00168Rome,Italy e-mail:[email protected] #TheSocietyforExperimentalMechanics,Inc.2017 1 C.S.Korachetal.(eds.),MechanicsofBiologicalSystemsandMaterials,Volume6, ConferenceProceedingsoftheSocietyforExperimentalMechanicsSeries,DOI10.1007/978-3-319-41351-8_1
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