MMMMMMMaaaaaaannnnnnnaaaaaaagggggggeeeeeeemmmmmmmeeeeeeennnnnnnttttttt ooooooofffffff cccccccooooooollllllllllllllaaaaaaarrrrrrr rrrrrrrooooooottttttt ooooooofffffff ccccccchhhhhhhiiiiiiiccccccckkkkkkkpppppppeeeeeeeaaaaaaa bbbbbbbyyyyyyy nnnnaaaattttiiiivvvveeee iiiissssoooollllaaaatttteeeessss ooooffff TTTrrriiiccchhhooodddeeerrrmmmaaa BByy MMMMMMdddddd...... SSSSSShhhhhhaaaaaahhhhhhzzzzzzaaaaaammmmmmaaaaaannnnnn AAAAAAhhhhhhssssssaaaaaannnnnn MMMMMMMaaaaaaasssssssttttttteeeeeeerrrrrrr ooooooofffffff SSSSSSSccccccciiiiiiieeeeeeennnnnnnccccccceeeeeee iiiiiiinnnnnnn AAAAAAAgggggggrrrrrrriiiiiiicccccccuuuuuuullllllltttttttuuuuuuurrrrrrreeeeeee ((((PPPPllllaaaannnntttt PPPPaaaatttthhhhoooollllooooggggyyyy)))) DDDDDDEEEEEEPPPPPPAAAAAARRRRRRTTTTTTMMMMMMEEEEEENNNNNNTTTTTT OOOOOOFFFFFF PPPPPPLLLLLLAAAAAANNNNNNTTTTTT PPPPPPAAAAAATTTTTTHHHHHHOOOOOOLLLLLLOOOOOOGGGGGGYYYYYY DDrr.. RRAAJJEEEEEENNNNNNDDDDDDRRRRRRAAAAAA PPPPPPRRRRRRAAAAAASSSSSSAAAAAADDDDDD CCCCCCEEEEEENNNNNNTTTTTTRRRRRRAAAAAALLLLLL AAAAAAGGGGGGRRRRRRIIIIIICCCCCCUUUUUULLLLLLTTTTTTUUUUUURRRRRRAAAAAALLLLLL UUUUUUUNNNNNNNIIIIIIIVVVVVVVEEEEEEERRRRRRRSSSSSSSIIIIIIITTTTTTTYYYYYYY,,,,,,, BBBBBBBIIIIIIIHHHHHHHAAAAAAARRRRRRR,,,,,,, PPPPPPPUUUUUUUSSSSSSSAAAAAAA (((((((SSSSSSSAAAAAAAMMMMMMMAAAAAAASSSSSSSTTTTTTTIIIIIIIPPPPPPPUUUUUUURRRRRRR))))))) – 884488 112255 IINNDDIIAA 22001166 RReegg.. NNoo.. – MMMM////PPPPPPPP////111144444444////2222000011114444-15 MMMMMMMMaaaaaaaannnnnnnnaaaaaaaaggggggggeeeeeeeemmmmmmmmeeeeeeeennnnnnnntttttttt ooooooooffffffff ccccccccoooooooollllllllllllllllaaaaaaaarrrrrrrr rrrrrrrrooooooootttttttt ooooooooffffffff cccccccchhhhhhhhiiiiiiiicccccccckkkkkkkkppppppppeeeeeeeeaaaaaaaa bbbbbbbbyyyyyyyy nnnnaaaattttiiiivvvveeee iiiissssoooollllaaaatttteeeessss ooooffff TTTrrriiiccchhhooodddeeerrrmmmaaa BByy MMMMMMdddddd...... SSSSSShhhhhhaaaaaahhhhhhzzzzzzaaaaaammmmmmaaaaaannnnnn AAAAAAhhhhhhssssssaaaaaannnnnn AAAA TTTTHHHHEEEESSSSIIIISSSS SSSSUUUUBBBBMMMMIIIITTTTTTTTEEEEDDDD TTTTOOOO DDDDDDDrrrrrrr....... RRRRRRRAAAAAAAJJJJJJJEEEEEEENNNNNNNDDDDDDDRRRRRRRAAAAAAA PPPPPPPRRRRRRRAAAAAAASSSSSSSAAAAAAADDDDDDD CCCCCCCEEEEEEENNNNNNNTTTTTTTRRRRRRRAAAAAAALLLLLLL AAAAAAAGGGGGGGRRRRRRRIIIIIIICCCCCCCUUUUUUULLLLLLLTTTTTTTUUUUUUURRRRRRRAAAAAAALLLLLLL UUUNNNIIIVVVEEERRRSSSIIITTTYYY, PPUUSSAA IIIIINNNNN PPPPPAAAAARRRRRTTTTTIIIIIAAAAALLLLL FFFFFUUUUULLLLLFFFFFIIIIILLLLLLLLLLMMMMMEEEEENNNNNTTTTT OOOOOFFFFF TTTTTHHHHHEEEEE RRRRRRREEEEEEEQQQQQQQUUUUUUURRRRRRREEEEEEEMMMMMMMEEEEEEENNNNNNNTTTTTTTSSSSSSS FFFFFFFOOOOOOORRRRRRR TTTTTTTHHHHHHHEEEEEEE AAAAAAAWWWWWWWAAAAAAARRRRRRRDDDDDDD OOOOOOOFFFFFFF TTTTTTTHHHHHHHEEEEEEE DDDDDDDEEEEEEEGGGGGGGRRRRRRREEEEEEEEEEEEEE OOOOOOOFFFFFFF MMMMMMMaaaaaaasssssssttttttteeeeeeerrrrrrr ooooooofffffff SSSSSSSccccccciiiiiiieeeeeeennnnnnnccccccceeeeeee iiiiiiinnnnnnn AAAAAAAgggggggrrrrrrriiiiiiicccccccuuuuuuullllllltttttttuuuuuuurrrrrrreeeeeee ((((PPPPllllaaaannnntttt PPPPaaaatttthhhhoooollllooooggggyyyy)))) DDDDDDEEEEEEPPPPPPAAAAAARRRRRRTTTTTTMMMMMMEEEEEENNNNNNTTTTTT OOOOOOFFFFFF PPPPPPLLLLLLAAAAAANNNNNNTTTTTT PPPPPPAAAAAATTTTTTHHHHHHOOOOOOLLLLLLOOOOOOGGGGGGYYYYYY DDDDrrrr.... RRRRAAAAJJJJEEEENNNNDDDDRRRRAAAA PPPPRRRRAAAASSSSAAAADDDD CCCCEEEENNNNTTTTRRRRAAAALLLL AAAAGGGGRRRRIIIICCCCUUUULLLLTTTTUUUURRRRAAAALLLL UUUUUUUNNNNNNNIIIIIIIVVVVVVVEEEEEEERRRRRRRSSSSSSSIIIIIIITTTTTTTYYYYYYY,,,,,,, BBBBBBBIIIIIIIHHHHHHHAAAAAAARRRRRRR,,,,,,, PPPPPPPUUUUUUUSSSSSSSAAAAAAA (((((((SSSSSSSAAAAAAAMMMMMMMAAAAAAASSSSSSSTTTTTTTIIIIIIIPPPPPPPUUUUUUURRRRRRR))))))) – 884488 112255 IINNDDIIAA 2200116 RReegg. No. –– M///PPPPPP///111444444///222000111444-115 Md. Shahzaman Ahsan…. Acknowledgement It is a matter of gratitude and euphoria to grace back and recall the path one travels during the days of hard work and pre-perseverance and it is still great at this juncture to recall the faces and spirit in the form of parents, teachers, friends, near and dear ones. I would consider this work nothing more than incomplete without attending to the task of acknowledging the overwhelming help I received during this endeavour of mine. Fervently and modestly, I express my deep sense of gratitude to Dr. J. P. Upadhyay, University professor, Department of Plant Pathology, Dr. Rajendra Prasad Central Agricultural University, Pusa, Bihar and Chairman of my advisory committee, for his valuable guidance, keen and continued interest and encouragement with constructive suggestions throughout the present investigation and in the preparation of this manuscript. I am immensely grateful to the distinguished members of my advisory Committee Dr. Sangita Sahni, Dr. Neeraj Kumar and Dean’s Nominee- Dr. N. K. Singh for their valuable suggestions and constructive criticism during the course of this investigation. I am immensely grateful to Dr. R. C. Rai, Dr. P. K. Jha, Dr. R. K. Ranjan, Dr. C. S. Chaudhary, Dr. S. K. Singh, Dr. Minnatullah, Dr. Phool chand and Dr. B. Kumar for their valuable suggestions and constructive criticism during the course of this investigation. I would be failing in my duties if I do not take this opportunity to express my sincere gratitude to Dr. (Mrs.) Bimla Rai chairperson, Department of Plant Pathology who assisted me in various ways throughout the research work. I express my gratitude to all teachers and staff members, Department of Soil Science, Dr. Rajendra Prasad Central Agricultural University, Pusa, Bihar for their advice, moral support, inspiration and stimulating encouragement. I express my heartfelt gratitude to my reverend father Late Md. Idris, Mother Late B. B. Mazlooman Khatoon, Brothers Md. Shahid Reza, Md. Shahnawaz Rezvi, Md. Shahzad Anwar, and Md. Shahbaj Alam, Sisters Shahnaz Perween, and Shahina Perween for their unite help, love, affection, sacrifices untiring help, constant inspiration and making memorably strong without which I could not materialize this dream. I wish to record my special thanks to my juniors and seniors Shashimala Kumari, Paritosh Kumar, Rahul Kumar, Rahul Maurya, Pankaj Ojha, Abhinandan singh, Vijay kumar, Chaitanya, Md. Akram, Abhay, Prince, Pankaj, Prasoon, Komal, Priyanka and my friends Atul Kumar, Vipul Mishra, Indrajeet kumar, Prabhat Kiran, Ramayan Kushwaha, Nitesh, Prakash Chand, Alok kumar, Shashi kumar, Pankaj Kesari, Subhash kumar and Kuldeep Sharma who helped me in various ways during the course of present investigation. Lastly, I bow at the feet of “Allah” with whose omnipresent blessing today on the eve of completion of my thesis. This is a long adventurous journey to the unknown destination with a hope for future. I was not alone in this journey, to accomplish this Hercules task. Today is one of delightful moments of my life. I am in position to acknowledge all those, who helped me a lot to cross the way, in finishing the marathon work. CONTENTS Sl. PAGE PARTICULARS No. NO. 1. INTRODUCTION 1-4 2. REVIEW OF LITERATURE 5-22 3. MATERIALS AND METHODS 23-36 4. EXPERIMENTAL RESULTS 37-52 4.1 Soil texture, pH and organic carbon content in soil samples 37 4.2 Enumeration of total fungal population in soil 37-38 4.3 Identification of Trichoderma 38-39 4.4 Cultural and morphological characters of S. rolfsii 41 4.5 Screening of T. harzianum isolates against S. rolfsii 41 4.6 Mycoparasitism of Trichoderma harzianum 4 on S. rolfsii 42 4.7 Viability test of mycelium of S. rolfsii in dual culture 42 4.8 Formation of sclerotia by S. rolfsii in dual culture 43 4.9 Viability test of sclerotia of S. rolfsii in dual culture 44 4.10 In vitro evaluation of different fungicides against Sclerotium 45 rolfsii 4.11 Tolerance of different fungicides by T. harzianum isolate 4 in 46 vitro 4.12 Screening of chickpea varieties against collar rot in pot 47 4.13 Effect of different doses of T. harzianum on management of 48 collar rot of chickpea 4.14 Fungicidal control of collar rot 49 4.15 Integration of fungicide and T. harzianum isolate 4 for 50-52 management of collar rot of chickpea 5. DISCUSSION 53-61 6. SUMMARY AND CONCLUSION 62-64 BIBLIOGRAPHY i-xvii
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