Mass Spectrometry–Based Glycoproteomics and Its Clinic Application Mass Spectrometry–Based Glycoproteomics and Its Clinic Application Edited by Haojie Lu First edition published 2022 by CRC Press 6000 Broken Sound Parkway NW, Suite 300, Boca Raton, FL 33487-2742 and by CRC Press 2 Park Square, Milton Park, Abingdon, Oxon, OX14 4RN © 2022 selection and editorial matter, Haojie Lu; individual chapters, the contributors CRC Press is an imprint of Taylor & Francis Group, LLC Reasonable efforts have been made to publish reliable data and information, but the author and publisher cannot assume responsibility for the validity of all materials or the consequences of their use. The authors and publishers have attempted to trace the copyright holders of all material reproduced in this publication and apologize to copyright holders if permission to publish in this form has not been obtained. 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Library of Congress Cataloging‑in‑Publication Data Names: Lu, Haojie, 1974- editor. Title: Mass spectrometry based glycoproteomics and its clinic application / edited by Haojie Lu. Description: First edition. | Boca Raton, FL : CRC Press, 2021. | Includes bibliographical references and index. | Summary: “This book demonstrates the progress that has been achieved in Mass spectrometry based glycoproteomic analysis, including a wide range of analytical methodologies and strategies involved in selective enrichment, qualitative analysis, quantitative analysis and data analy.sis, together with their clinical applications. The book will serve as a valuable resource to aid appropriate techniques and their applications for students, postdocs, and researchers working in proteomics, glycoscience, analytical chemistry, biochemistry and clinical medicine”-- Provided by publisher. Identifiers: LCCN 2021001208 (print) | LCCN 2021001209 (ebook) | ISBN 9781032028613 (hardcover) | ISBN 9781003185833 (ebook) Subjects: LCSH: Glycoproteins--Analysis. | Mass spectrometry--Methodology. Classification: LCC QP552.G59 M35 2021 (print) | LCC QP552.G59 (ebook) | DDC 572/.68--dc23 LC record available at https://lccn.loc.gov/2021001208 LC ebook record available at https://lccn.loc.gov/2021001209 ISBN: 978-1-032-02861-3 (hbk) ISBN: 978-1-032-02923-8 (pbk) ISBN: 978-1-003-18583-3 (ebk) DOI: 10.1201/9781003185833 Typeset in Minion by KnowledgeWorks Global Ltd. Contents Editor, vii Contributors, ix Chapter 1 ◾ S elective Enrichment Methods for N-Glycosite Containing Peptides in N-Glycoproteomics 1 Guoli WanG, YinG ZhanG, and haojie lu Chapter 2 ◾ Q uantitative Methods for N-Glycosite Containing Peptides in N-Glycoproteomics 29 ZhenYu Sun, YinG ZhanG, and haojie lu Chapter 3 ◾ Q ualitative and Quantitative Methods for N-Glycans in N-Glycomics 55 ShifanG ren and haojie lu Chapter 4 ◾ Q ualitative and Quantitative Analytical Methods for Intact Glycopeptides 91 Weiqian Cao and penGYuan YanG Chapter 5 ◾ A nalytical Software and Databases in N-Glycoproteomics 131 SuidenG qin and Zhixin tian Chapter 6 ◾ C linical Applications 167 CaiYun fanG and haojie lu INDEX, 237 v Editor Haojie Lu, PhD, is a Professor at Fudan University since 2003. He earned his PhD from Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences in 2001. He specializes in proteomics based on mass spectrom- etry with particular emphasis on novel technologies for separation and identification of low-abundant proteins and post-translationally modified proteins (including glycosylation), as well as relative and absolute quan- tification methods for proteomics. Dr. Lu’s awards include 2009 Winner of Young Chemist Award from China Chemical Society; 2009 University Distinguished Professor of Shanghai (Eastern Scholar); 2010 supported by The National Science Fund for Distinguished Young Scholars; and 2013 he was awarded the Eastern Scholar tracking program. He has authored articles published in national and international journals. vii Contributors Weiqian Cao Suideng Qin The Fifth People’s Hospital, Fudan School of Chemical Science University, and The Shanghai & Engineering Key Laboratory of Medical Shanghai Key Laboratory of Epigenetics Chemical Assessment and The International Co-laboratory Sustainability of Medical Epigenetics and Tongji University Metabolism Shanghai, China Ministry of Science and Technology Institutes of Biomedical Sciences Shifang Ren NHC Key Laboratory of NHC Key Laboratory of Glycoconjugates Research Glycoconjugates Research Fudan University Department of Biochemistry Shanghai, China and Molecular Biology School of Basic Medical Sciences Caiyun Fang Fudan University Department of Chemistry Shanghai, China Fudan University Shanghai, China Zhenyu Sun Institutes of Biomedical Sciences Haojie Lu Fudan University Department of Chemistry Shanghai, China Institutes of Biomedical Sciences NHC Key Laboratory of Glycoconjugates Research, and Shanghai Cancer Center Fudan University Shanghai, China ix