Making and Using Antibodies: A Practical Handbook

410 Pages·2006·11.16 MB·English

by  Gary C. Howard

Making
using
AND
antibodies
A practical handbook
Edited by
gary C. Howard
matthew R. Kaser
Boca Raton London New York
CRC Press is an imprint of the
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© 2007 by Taylor & Francis Group, LLC
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International Standard Book Number-10: 0-8493-3528-0 (Softcover)
International Standard Book Number-13: 978-0-8493-3528-0 (Softcover)
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Library of Congress Cataloging-in-Publication Data
Making and using antibodies : a practical handbook / edited by Gary C. Howard
and Matthew R. Kaser.
p. cm.
Includes bibliographical references and index.
ISBN 0-8493-3528-0 (alk. paper)
1. Immunoglobulins--Handbooks, manuals, etc. I. Howard, Gary C. II. Kaser,
Matthew R.
QR186.7.M35 2006
571.9’67--dc22 2006047535
Visit the Taylor & Francis Web site at
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3528_Prelims.fm Page iii Wednesday, October 25, 2006 1:42 PM
Dedication
This book is dedicated to
Rebecca and Amanda Howard
G.C.H.
and to
Michael and Elizabeth Kaser,
Margery Ord, and Lloyd Stocken
M.R.K.
3528_Prelims.fm Page iv Wednesday, October 25, 2006 1:42 PM
3528_bookTOC.fm Page v Friday, October 27, 2006 1:40 PM
Table of Contents
Chapter 1
Antibodies..................................................................................................................1
Matthew R. Kaser and Gary C. Howard
Chapter 2
Antigens.....................................................................................................................7
Paul Algate, Jory Baldridge, and Sally Mossman
Chapter 3
Adjuvants.................................................................................................................27
Jory Baldridge, Paul Algate, and Sally Mossman
Chapter 4
Production of Polyclonal Antibodies......................................................................41
Lon V. Kendall
Chapter 5
Production of Monoclonal Antibodies....................................................................73
Kathleen C. F. Sheehan
Chapter 6
Quantitative Production of Monoclonal Antibodies...............................................95
David A. Fox and Elizabeth M. Smith
Chapter 7
Purification and Characterization of Antibodies...................................................125
Joseph P. Chandler
Chapter 8
Making Antibodies inBacteria..............................................................................157
Frederic A. Fellouse and Sachdev S. Sidhu
Chapter 9
Chemical and Proteolytic Modification of Antibodies.........................................181
George P. Smith
3528_bookTOC.fm Page vi Friday, October 27, 2006 1:40 PM
Chapter 10
Applications...........................................................................................................247
Lee Bendickson and Marit Nilsen-Hamilton
Chapter 11
Immunohistochemical Methods............................................................................273
José A. Ramos-Vara and Julie Ackerman Saettele
Chapter 12
Immunoelectron Microscopy.................................................................................315
Sara E. Miller and David N. Howell
Chapter 13
Flow Cytometry.....................................................................................................339
Kristi R. Harkins and M. Elaine Kunze
Chapter 14
ELISAs...................................................................................................................361
John Chen and Gary C. Howard
Chapter 15
Antibodies in the Future: Challenges and Opportunities.....................................371
Matthew R. Kaser and Gary C. Howard
Index......................................................................................................................377
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Preface
Antibodies are perhaps one of the most extraordinary species of protein ever to
evolve during the history of life on Earth.These “magic bullets” have become an
indispensable tool in the study of biology and medicine. In biology, they have been
a key component of the surge in fundamental knowledge that has occurred in the
last quarter century. In the practice of medicine, multiple vaccines have led to the
control (at least in the developed world) of many infectious diseases, such as polio,
mumps, measles, chicken pox, and the almost total eradication of smallpox.
We hope this book will be useful to biomedical researchers and students.
Although new methods for making and using antibodies will certainly be found,
their current applications—ELISAs, Western blotting, immunohistochemistry, and
flow cytometry—are so powerful that they will remain critical to biomedical science
for a considerable period.
We want to thank the contributors to this volume. Their professional knowledge,
excellent writing, and enthusiastic support made the book possible. We also owe
great thanks to our editor at CRC Press, Judith Spiegel, for her valuable help and
great patience with this project.
Matthew R. Kaser and Gary C. Howard
Castro Valley, California
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Editors
Matthew R. Kaser, D.Phil. earned his D.Phil. in biochemistry from Oxford
University (UK) in 1988. After postdoctoral positions at the University of California,
the University of Texas, and at REI Harbor-UCLA Medical Center, he was appointed
to a faculty position at the University of California, San Francisco, Department of
Pediatrics and then served as a scientist and patent agent at Incyte Genomics in Palo
Alto, California. Dr. Kaser has been practicing as a patent agent since 1999, was
associate director of intellectual property at Mendel Biotechnology, and is now a
senior partner at Bell & Associates in San Francisco. He has presented research
papers at a number of regional, national, and international conferences and coau-
thored more than a dozen publications.
Gary C. Howard, Ph.D. earned his Ph.D. in biological sciences from Carnegie
Mellon University in 1979. He completed his postdoctoral training at Harvard
University and The Johns Hopkins University and was a research assistant biochem-
ist at the University of California, San Francisco. He then joined Vector Laboratories
in Burlingame as a biochemist and Medix Biotech (a subsidiary of Genzyme) in
Foster City, California, as chemistry manager and operations manager. Currently,
he is principal scientific editor at the J. David Gladstone Institutes, a private bio-
medical research institute affiliated with the University of California, San Francisco.
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