Lipids Part D

876 Pages·1981·14.685 MB·English

by  John M. Lowenstein (Eds.)

Contributors to Volume 72
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
R. G. ACKMAN (11, 12), Fisheries Research Medical Center and ehT Graduate
and Technology Laboratory, Technical School, Washington, D.C. 70002
University of Nova Scotia, P.O. Box DAVID P. BLOXHAM (47), Department of
,0001 Halifax, Nova Scotia B3J 2X4, Biochemistry, School of Biochemical and
Canada Physiological Sciences, University of
DONALD O. ALLEN (58), Department of Southampton, Southampton S09 3TU,
Pharmacology, University of South United Kingdom
Carolina School of Medicine, Columbia, RAGROB BORREaAEK (38), Institute of Medi-
South Carolina 80292 cal Biochemistr3,, University of Oslo, Oslo
MARIETTA L. YKSN1GAB (21), Department ,3 Norway
of Cellular Biology, Scripps Clinic and JaN BREMER (38), Institute of Medical
Research Foundation, La Jolla, Califor- Biochemistry, University of Oslo, Oslo ,3
ain 73029 Norway
FRED. C. BAKER (5), Department of HENRI BRUNENGRABER (2), Department of
Biochemistry, Zoecon Corporation, DiaP Nutrition and Food Science, Massachu-
Alto, California 40349 setts Institute of Technology. Cambridge,
ANTHONY J. BARAK (14), Liver Studs, Unit, Massachusetts 93120
Veterans Administration Medical Center, STEFANIE CANN (2), Department of
and the Departments of Internal Medicine Biochemistry, Dartmouth Medical
and Biochemistry, University of Nebraska School, Hanover, New Hampshire 55730
Medical Center, Omaha, Nebraska 6810f A. CESTELLI (26), Istituto id Anatomia
ROLAND E. BARDEN (44), Departments of Comparata, 32109 Palermo, Italy
Chemistry and Biochemistry, University R. ALAN CHALKLEY (47), Department of
of Wyoming, Laramie, Wyoming 17028 Biochemistry', School of Biochemical and
Y. BARENHOLZ (24), Laboratory of Neuro- Physiological Sciences, University of
chemistry, Department of Biochemistry, Southampton, Southampton S09 3TU,
Hebrew University-Hadassah Medical United Kingdom
School, P.O. Box ,2711 Jerusalem ,00019 WEN-SHERNG CHEN (46), Kraft Inc., Re-
Israel search and Development Center, Glen-
PER BELFRAGE (20), Department of Physio- view, Illinois 52006
logical Chemistry 4, University of Lund, RENATA Z. NESNA1TSIRHC (38), Institute of
P.O. Box 750, S-220 70 Lund, Sweden Medical ,'.3rtsimehcoiB University of Oslo,
G. BESLEY (24), Department of Pathology, Oslo ,3 Norway
Royal Hospital for Sick Children, Edin- DAVID P. CLARK (57), Department of -iM
burgh EH9 ILF, United Kingdom crobiology, University tf lllinois, Urbana.
L. L. BIEBER (13, 16), Department of Illinois 10816
Biochemisto', Michigan State University, PETER R. CLEMENTS (43, 44), Department
East Lansing, Michigan 42884 of Physiological Chemistry, The Johns
DAVID BILLINGTON (49), Department of Hopkins University, Baltimore, Maryland
Chemistry and Biochemistry, Liverpool 50212
Polytechnic, Liverpool L3 3AF, United C. E. COLSON (6), Graduate Department of
Kingdom Biochemistry, Brandeis University,
MELVIN BLECHER (28), Department of Waltham, Massachusetts 45220
Biochemistry, Georgetown University ALI RIADS M. COOK (15), Mikrobiologisches
xii CONTRIBUTORS TO VOLUME 72
lnstitut, Eidgen6ssische Technische Research in Dairying, Shinfield, Reading
Hochschule, CH-8092 Ziirich, Switzer- RG2 9AT, United Kingdom
land R. A. FREEDLAND (37), Department of
GARY COOPER (47), Department of Physiological Sciences, School of Veteri-
Biochemistry, School of Biochemical and nary Medicine, University of California,
Physiological Sciences, University of Davis, California 95616
Southampton, Southampton S09 3TU, HANSrJOACHIM GALLA (33), Department of
United Kingdom Biophysics, University of UIm, D-7900
E. COSTANTINO-CECCARINi (26), lstituto In- Ulm, Federal Republic of Germany
ternazionale Genetica e Biofisica, 52108 JEFFREY L. GARWIN (27), Department of
Napoli, Italy Microbiology and Molecular Genetics,
JOHN E. ,NANORC JR. (27, 57), Department Harvard Medical School, Boston, Mas-
of Microbiology, University of Illinois, sachusets 83120
Urbana, Illinois 10816 SH1MON GATT (24, 25), Laboratory of
CHRISTIAN G. DAUGHTON (15), Sanitary Neurochemistry, Department of Bio-
Engineering Research Laboratory, Uni- chemistry, Hebrew University-Hadassah
versity of California, Berkeley, Richmond, Medical School, P.O. Box 1172, Jeru-
California 40849 salem ,00019 Israel
R. J. DESNICK (24), Division of Medical -eG R. GOLDBERG (24), Laboratory of Neuro-
netics, Department of Pediatrics, Mount chemistry, Department of Biochemistry,
Sinai Hospital School of Medicine, New Hebrew University-Hadassah Medical
York, New York 92001 School, P.O. Box ,2711 Jerusalem ,00019
E. A. DEVINE (24), Division of Medical -eG Israel
netics, Department of Pediatrics, Mount ROBERT G. GRIFFIN (8), Francis Bitter Na-
Sinai Hospital School of Medicine, New tional Magnet Laboratory, Massachu-
York, New York 92001 setts Institute of Technology, Cambridge,
RAYMOND DIES (61), Department of Phys- Massachusetts 93120
iology and Biochemistry, University of ENNAZUcS M. HAAS (16), 498 Cedar Street,
Reading, Whiteknights, Reading RG6 Marine City, Michigan 93084
2A J, United Kingdom ANTHONY J. HANCOCK (54), Department of
T. DIN Jt R (24), Laboratory of Neurochemis- Chemistry and School of Medicine, Uni-
,'Ot Department of Biochemistry, Hebrew versity of Missouri, Kansas City, Missouri
University-Hadassah Medical School, 01146
P.O. Box ,2711 Jerusalem ,00019 Israel ROBERT A. HARRIS (41), Department of
HANSJORG ElSE (53), Max-Planck-lnstitut Biochemistry, Indiana University School
fiir Biophysikalische Chemie, D-3400 of Medicine, Indianapolis, Indiana
GOttingen-Nikolausberg, Federal Repub- 32264
cil of Germany WOLFGANG HARTMANN (33), Department
AKIRO ENDO (56), Department of Agricul- of Biophysics, University of Ulm, 0097-D
tural and Biological Chemistry, Tokyo Ulm, Federal Republic of Germany
Noko University, Fucha-shi, Tokyo ,381 MICHAEL HELLER (22), Department of
Japan Biochemistry, Myocardial Research
WARREN L. ERDAHL (7), The Hormel Insti- ,puorG ehT Hebrew University-
tute, University of Minnesota, Austin, Hadassah Medical School, P.O. Box
Minnesota 21955 ,2711 Jerusalem ,00019 Israel
JIM C. FONG (48), Department of Psychi- Louis B. HERSH (45), Department of
atry, New York University Medical Biochemistry, The University of Texas
Center, New York, New York 61001 Health Science Center at Dallas, Dallas,
ISAaEL A. FORSYTH (61), Department of Texas 53257
Physiology, National Institute for W. Ho (40), Department of Chemical Re-
CONTRIBUTORS TO VOLUME 72 xiii
search, McNeil Pharmaceutical, Spring DOUGLAS S. LEWIS (63), Cardiopulmonary
House, Pennsylvania 77491 Diseases, Southwest Foundation for Re-
PAUL C. HOLLAND (50), Biochemistry -eD search and Education, San Antonio,
partment, University of Saskatchewan, Texas 48287
Saskatoon, Saskatchewan S7N ,OWO MARCEL S. F. LIE KEN JIE (32), Depart-
Canada ment of Chemistry, University of Hong
ANTHONY H. C. HUANG (67), Department Kong, Pokfulam Road, Hong Kong
of Biology, University of South Carolina, NYRHTAK J. LONG (58), Department of
Columbia, South Carolina 80292 Pharmacology, University of South
BRUCE S. HUDSON (34, 35), Department of Carolina School of Medicine, Columbia,
Chemistry, University of Oregon, South Carolina 80292
Eugene, Oregon 50479 JOHN M. LOWENSTEIN (2, 6, 36, 42), De-
VASUDEV C. JOSHI (62), Marrs McLean De- partment of Biochemistry, Brandeis
partment of Biochemistry, Baylor College University, Waltham, Massachusetts
of Medicine, Houston, Texas 03077 45220
D. BRUCE KEECH (43), Department of J. J. MACCARTHY (64), Department of -DiB
Biochemistry, University of Adelaide, chemistry and Biophysics, University of
Adelaide, South Australia 1005 California, Davis, ainr~ifilaC 61659
CLAUDIA KENT (23), Department of SYLVIA A. McCUNE (41), Department of
Biochemistry, Purdue University, West Biochemistry, Indiana University School
Lafayette, Indiana 70974 of Medicine, Indianapolis, Indiana 32264
H. GOSIND KHORANA (29), Departments of MARY ANN K. MARKWELL (16), Depart-
Biology and Chemistry, Massachusetts ment of Microbiology and Molecular -iB
Institute of Technology, Cambridge, Mas- ology Institute, University of ,ainrbCilaC
sachusetts 93120 Los Angeles, California 42009
P. E, KOLATTUKUDY (59), Institute of -oiB EDWARD J. MASORO (63), Department of
logical Chemistry and Biochemistry/ Physiology, University of Texas Health
Biophysics Program, Washington State Science Center, San Antonio, Texas
University, Pullman, Washington 46199 48287
STEPHAN KOVATCHEV (53), Max-Planck- R. J. MOHRBACHER (40), Department of
lnstitut fiir Biophysikalische Chemie, Chemical Research, McNeil Pharmaceu-
0043-D Gottingen-Nikolausberg, Federal tical, Spring House, Pennsylvania 77491
Republic of Germany DENIS J. Mt~RPHY (65), Department of -DiB
EDWARD L. KRUG (23), Department of chemistry and Biophysics, University of
Biochemisry, Purdue University, West California, Davis, ainrbJilaC 95616
Lafayette, Indiana 70974 REGOR S. NEWTON (37), Department of
D. N. KUHN (66), Department of Biochemistry, Michigan State University,
Biochemistry and Biophysics, University East Lansing, Michigan 42844
of California, Davis, California 61659 NILS 0STEN NILSSON (20), Department of
SAMAR K. KUNDU (9, 10), Department .fo Physiological Chemistry, University of
Medicine, Baylor College of Medicine, Lund, P.O. Box 750, S-220 70 Lund,
Houston, Texas 03077 Sweden
PAUL B. LAZAROW (19), ehT Rockefeller SATOSHI OMURA (39), School of Phar-
University, New York, New York 12001 maceutical Sciences, Kitasato University,
Z. LEIBOVITZ-BEN GERSHON (24), Pediat- Minato-ku, Tokyo 108, Japan
rics Research Unit, Hadassah University HARALD OSMUNDSEN (18, 38), Institute "fo
Hospital, Jerusalem ,00019 Israel Medical Biochemistry, University of
L. M. LEWIN (13), Department of Chemical Oslo, P.O. Box 1112, Blindern, Oslo ,3
Pathology, Sackler Medical School, leT Norway
Aviv University, leT Hashomer, Israel AHTRAM S. OWENS (44), Department of
xiv CONTRIBUTORS TO VOLUME 72
Biochemistry, University of Wyoming, ENZO SANTANIELLO (30), lstituto id
Laramie, Wyoming 17028 Chimica, Facolt~ id Medicina, Universit~
GEORGE M. PATTON (2), Veterans Adminis- di Milano, 33102-1 Milan, Italy
tration Hospital, Room 124, Research JOCHEN SCHACHT (52), Department of Bio-
Building, Boston, Massachusetts 03120 logical Chemistry and Kresge Hearing
ECYRB V. PLAPP (46), Department of Research Institute, University of Michi-
Biochemistry, The University of Iowa, gan, Ann Arbor, Michigan 90184
Iowa City, Iowa 24225 DAVID A. SCHOOLEY (5), Department of
R. D. PLATTNER (3), Northern Regional Re- Biochemistry, Zoecon Corporation, Paid
search Center, Agricultural Research Alto, California 40349
Science and Education Administration, HORST SCHULZ (48), Department of
Peoria, Illinois 40616 Chemistry, City College of the City Uni-
NED A. PORTER (4), Department of Chemis- versity of New York, New York, New York
try, Paul M. Gross Chemical Laborato- 13001
ries, Duke University, Durham, North J-L. SEBED~O (12), Fisheries Research and
Carolina 60772 Technology Laboratory, Technical Uni-
ORVILLE S. PRIVETT (7), The Hormel Insti- versity of Nova Scotia, P.O. Box ,0001
tute, University of Minnesota, Austin, Halifax, Nova Scotia B3J ,4(32 Canada
Minnesota 21955 KASTURI SENGUPTA (58), Department of
BRUCE M. RAAKA (42), Department of Med- Pharmacology, University of South
icine, New York University Medical Carolina School of Medicine, Columbia,
Center, New York, New York 61001 South Carolina 80292
NAHCAMAR DRAN RADHAKRISHNAN (29), B. SHAFIT-ZAGARDO (24), Division of Medi-
Departments of Biology and Chemistry, cal Genetics, Department of Pediatrics,
Massachusetts Institute of Technology, Mount Sinai Hospital School of Medicine,
Cambridge, Massachusetts 93120 New York, New York 92001
NAMRON S. RADIN (1, 51, 55), Mental H. STANLEY A. SHERRATT (49, 50), De-
Health Research Institute (Department of partment of Pharmacological Sciences,
Psychiatry) and Department of Biological The Medical School, University of New-
Chemistry, University of Michigan, Ann castle upon Tyne, Newcastle upon Tyne
Arbor, Michigan 90184 NEI 7RU, United Kingdom
W. N. RATNAYAKE (12), Fisheries Research ROaERT D. SIMONI (34, 35), Department of
and Technology Laboratory, Technical Biological Sciences, Stanford University,
University of Nova Scotia, P.O. Box Stanford, California 50349
I000, Halifax, Nova Scotia B3J 2X4, LARRY A. SKLAR (34), Scripps Clinic and
Canada Research Foundation, La Jolla, Califor-
ROBERT J. ROBSON (29), Departments of -iB ain 73029
ology and Chemistry, Massachusetts In- DANIEL J. SMITH (45), Department of "
stitute of Technology, Cambridge, Mas- Chemistry, University of Akron, Akron,
sachusetts 93120 Ohio 52344
CHARLES O. ROCK (27), Department of P. K. STUMPF (64, 65, 66), Department of
Biochemistry, St. Jude Children's Biochemistry and Biophysics, University
Research Hospital, Memphis, Tennessee of California, Davis, California 61659
10183 FRANK F. SUN (31), Experimental Biology
J. ROSENTHAL (24), Pediatrics Research Research, The Up john Company,
Unit, Hadassah University Hospital, Kalamazoo, Michigan 10094
Jerusalem ,00019 Israel HORATHOY TAKAGAKI (29), Departments of
THOMAS R. RUSSELL (60), Department of Biology and Chemistry, Massachusetts
Biochemistry, University of Miami School Institute of Technology, Cambridge,
of Medicine, Miami, Florida 10133 Massachusetts 93120
CONTRIBUTORS TO VOLUME 72 xv
N. E. TOLBERT (16), Department of ALAN H. ULLMAN (17), Industrial Chemi-
Biochemistry, Michigan State University, cals Division, ehT Procter & Gamble
East Lansing, Michigan 42884 Company, Cincinnati, Ohio 14254
AMY TSAI (35), Department of Biological RoY B. VE.RDERY III (25), Laboratory of
Sciences, Stanford University, Stanford, Lipoprotein Metabolism, Clinical Re-
California 50349 search Institute "fo Montreal, Montreal,
F. TSURUKI (24), Laboratoo, of Neuro- Quebec H2W IR7, Canada
chemistry, Department of Biochemistry, RANGA RAO VUr~NAM (55), Becton Dickin-
Hebrew University-Hadassah Medical son Diagnostics, Orangeburg. New York
School, P.O. Box ,2711 Jerusalem ,00019 26901
Israel JOHN C. WALLACE (43), Department of
DEAN J. TUMA (14), Liver Study Unit, Vete- Biochemistry, University of Adelaide,
rans Administration Medical Center, and Adelaide, Australia
the Departments of lnternal Medicine and HUGO WEEYEN (4), Department of Chemis-
Biochemistry, University of Nebraska try, Paul M. Gross Chemical Laborato-
Medical Center, Omaha, Nebraska 50186 ries, Duke University, Durham, North
G. F. TUTWILER (40), Department of Bio- Carolina 60772
logical Research, McNeil Pharmaceuti- HAROLD B. WHITE Ill (17), Department of
cal, Spring House, Pennsylvania 77491 Chemistry, University of Delaware,
SmMON UHTZUR (22), Department of Newark, Delaware 1971l
Food Engineering and Biotechnology, BYUNG P. Yu (63), Department of Physiol-
Technion-Israel Institute fi)r Technology, ogy, University of Texas Health Science
Haifa, Israel Center, San Antonio, Texas 48287
Preface
Let me haue nem about me, that are fat
Sleek-headed men, and such as sleep a'nights
suiluJ Caesar Act I, Scene II*
Fat people are no longer the preferred stereotype of placidity and
health, and sleep is a somewhat overestimated commodity. Fat books, on
the other hand, proliferate. A single volume was planned originally to
cover recent developments in the biochemistry of lipids. It would have
been too obese for comfort, so it was decided to divide the book into two
volumes. If only obesity could be controlled so easily in the real world!
Volume 17 dealt with enzymes while Volume 72 covers methods use-
ful to the lipid biochemist. Each section starts with a list of related articles
previously published in the Methods in Enzymology series. In general, the
enzymes included have been purified highly. A notable exception are
enzymes of lipid metabolism from plants, a number of which are included
even though they have only been purified partially. It is hoped that their
inclusion will provide a stimulus to their isolation in homogeneous form.
The borderline between fatty acid and sterol metabolism has been set
at fl-hydroxy-fl-methylglutaryl-CoA. The inclusion of some enzymes may
strike the reader as odd, but there was in each case good reason. For
example, 3-methylcrotonyl-CoA carboxylase is included because of its
relation to acetyl-CoA carboxylase and its ability to carboxylate free
biotin, a valuable property for understanding acetyl-CoA carboxylase.
I welcome suggestions for future volumes. Please do not hesitate to
draw my attention to errors of omission or commission.
JOHN M. LOWENSTEIN
* H. .M Furness, Jr. (ed.) .)3191( J. .B Lippincott Co., Philadelphia and London, p. .54
iivx
METHODS IN ENZYMOLO(_iY
EDITED BY
Sidney P. Colowick and Nathan O. Kaplan
VANDERBILT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDICINE UNIVERSITY OF CALIFORNIA
NASHVILLE, TENNESSEE AT SAN DIEGO
LA JOLLA, CALIFORNIA
I. Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
III. Preparation and Assay of Substrates
IV. Special Techniques for the Enzymologist
.V Preparation and Assay of Enzymes
VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
VII. Cumulative Subject Index
xix
METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
Sidney P. Colowick Nathan O. Kaplan
VOLUME VIII. Complex Carbohydrates
Edited yb HTEBAZILE F. NEUFELD DNA ROTCIV GRUBSNIG
VOLUME IX. Carbohydrate Metabolism
Edited yb WILLIS A. DOOW
VOLUME X. Oxidation and Phosphorylation
Edited yb DLANOR W. KOORBATSE DNA DRANYAM E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited yb C. H. W. SR1H
EMULOV XII. Nucleic Acids (Parts A and B)
Edited yb ECNERWAL NAMSSORG DNA KlVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited yb J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited yb J. M. NIETSNEWOL
VOLUME .VX Steroids and Terpenoids
Edited yb DNOMYAR B. CLAYTON
VOLUME XVI. Fast Reactions
Edited yb KENNETH KUSTIN
VOLUME XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by HERBERT TABOR AND CELIA WHITE TABOR
VOLUME XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited yb DONALD B. MCCORMICK DNA LEMUEL D. WRIGHT
ixx
xxii METHODS IN ENZYMOLOGY
EMULOV XIX. Proteolytic Enzymes
Edited by EDURTREG E. NNAMLREP DNA LASZLO DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part )C
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXlI. Enzyme Purification and Related Techniques
Edited by WILLIAM B. YBOKAJ
EMULOV XXlII. Photosynthesis (Part A)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited yb ECNERWAL NAMSSORG DNA KIVlE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by SIDNEY REHCSIELF DNA LESTER REKCAP
EMULOV XXXII. Biomembranes (Part )B
Edited by SIDNEY FLEISCHER AND LESTER PACKER
METHODS 1N ENZYMOLOGY XXlll °..
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited yb AHTRAM G. SINNED DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited yb WILLIAM B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part B)
Edited yb JOHN M. NIETSNEWOL
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited yb BERT .W O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
detidE yb JOEL G. NAMDRAH DNA BERT .W O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and
Organ Systems)
Edited by JOEL G. NAMDRAH DNA BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part )B
Edited yb W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part )C
Edited yb W. A. DOOW
EMULOV XLIII. Antibiotics
Edited by JOHN H. HSAH
EMULOV XLIV. Immobilized Enzymes
Edited yb KLAUS HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part )B
Edited yb LASZLO DNAROL
EMULOV XLVI. Affinity Labeling
Edited yb WILLIAM B. YBOKAJ DNA R1EM KEHCLIW