Table Of Content

Media Connections With this edition, we introduce our new SaplingPlus with Lehninger teaching and learning platform. Its online homework system provides robust, high-level questions that you can assign to students for practice and assessment, with hints and wrong-answer feedback. Below is a chapter-by-chapter list of the other media resources available on the SaplingPlus with Lehninger platform. • The Interactive Metabolic Map, including tutorials and concept check questions, allows students to zoom between overview and detailed views of the most commonly taught metabolic pathways. • Case Studies (6, with more to be added) ask students to solve a biochemical mystery by choosing from different investigation options. • Molecular Structure Tutorials (9) guide students through concepts using three-dimensional molecular models, now updated with multiple-choice assessment. • Simulations (11) allow students to interact with structures and processes in a gamelike format. • Animated Mechanism Figures (29) show key reactions in detail. • Living Graphs (18) allow students to alter the parameters in key equations and graph the results. • Nature Articles with Assessment (6) provide an article from Nature plus tailored assessment to engage students in reading primary literature and to encourage critical thinking. • Animated Biochemical Techniques (9) illustrate the principles behind some of the most commonly used biochemical techniques. Chapter 2 Water Living Graphs: Henderson-Hasselbalch Equation Titration Curve for a Weak Acid Chapter 3 Amino Acids, Peptides, and Proteins UPDATED Molecular Structure Tutorial: Protein Architecture, section on amino acids Animated Biochemical Technique: SDS Gel Electrophoresis Chapter 4 The Three-Dimensional Structure of Proteins UPDATED Molecular Structure Tutorial: Protein Architecture, including sections on: Sequence and Primary Structure The α Helix The β Sheet The β Turn Introduction to Tertiary Structure Tertiary Structure of Fibrous Proteins Tertiary Structure of Small Globular Proteins Tertiary Structure of Large Globular Proteins Quaternary Structure Chapter 5 Protein Function UPDATED Molecular Structure Tutorials: Oxygen-Binding Proteins, including sections on: Myoglobin: Oxygen Storage Hemoglobin: Oxygen Transport Hemoglobin Is Susceptible to Allosteric Regulation Defects in Hb Lead to Serious Disease MHC Molecules Living Graphs: Protein-Ligand Interactions Binding Curve for Myoglobin Cooperative Ligand Binding Hill Equation Animated Biochemical Technique: Immunoblotting Chapter 6 Enzymes NEW Case Studies: Toxic Alcohols—Enzyme Function A Likely Story—Enzyme Inhibition UPDATED Animated Mechanism Figure: Chymotrypsin Mechanism Living Graphs: Michaelis-Menten Equation Competitive Inhibitor Uncompetitive Inhibitor Mixed Inhibitor Lineweaver-Burk Equation Chapter 8 Nucleotides and Nucleic Acids UPDATED Molecular Structure Tutorial: Nucleotides NEW Simulations: Nucleotide Structure DNA/RNA Structure Sanger Sequencing Polymerase Chain Reaction NEW Nature Article with Assessment: LAMP: Adapting PCR for Use in the Field Animated Biochemical Techniques: Dideoxy Sequencing of DNA Polymerase Chain Reaction Chapter 9 DNA-Based Information Technologies UPDATED Molecular Structure Tutorial: Restriction Endonucleases NEW Simulation: CRISPR NEW Nature Articles with Assessment: Assessing Untargeted DNA Cleavage by CRISPR/Cas9 Genome Dynamics during Experimental Evolution Animated Biochemical Techniques: Plasmid Cloning Reporter Constructs Synthesizing an Oligonucleotide Array Screening an Oligonucleotide Array for Patterns of Gene Expression Yeast Two-Hybrid Systems Chapter 11 Biological Membranes and Transport Living Graphs: Free-Energy Change for Transport (graph) Free-Energy Change for Transport (equation) Free-Energy Change for Transport of an Ion Chapter 12 Biosignaling UPDATED Molecular Structure Tutorial: Trimeric G Proteins Chapter 13 Bioenergetics and Biochemical Reaction Types Living Graphs: Free-Energy Change Free-Energy of Hydrolysis of ATP (graph) Free-Energy of Hydrolysis of ATP (equation) Chapter 14 Glycolysis, Gluconeogenesis, and the Pentose Phosphate Pathway NEW Interactive Metabolic Map: Glycolysis NEW Case Study: Sudden Onset—Introduction to Metabolism UPDATED Animated Mechanism Figures: Phosphohexose Isomerase Mechanism The Class I Aldolase Mechanism Glyceraldehyde 3-Phosphate Dehydrogenase Mechanism Phosphoglycerate Mutase Mechanism Alcohol Dehydrogenase Mechanism Pyruvate Decarboxylase Mechanism Chapter 16 The Citric Acid Cycle NEW Interactive Metabolic Map: The citric acid cycle NEW Case Study: An Unexplained Death—Carbohydrate Metabolism UPDATED Animated Mechanism Figures: Citrate Synthase Mechanism Isocitrate Dehydrogenase Mechanism Pyruvate Carboxylase Mechanism Chapter 17 Fatty Acid Catabolism NEW Interactive Metabolic Map: β-Oxidation NEW Case Study: A Day at the Beach—Lipid Metabolism UPDATED Animated Mechanism Figure: Fatty Acyl-CoA Synthetase Mechanism Chapter 18 Amino Acid Oxidation and the Production of Urea UPDATED Animated Mechanism Figures: Pyridoxal Phosphate Reaction Mechanisms (3) Carbamoyl Phosphate Synthetase Mechanism Argininosuccinate Synthetase Mechanism Serine Dehydratase Mechanism Serine Hydroxymethyltransferase Mechanism Glycine Cleavage Enzyme Mechanism Chapter 19 Oxidative Phosphorylation Living Graph: Free-Energy Change for Transport of an Ion Chapter 20 Photosynthesis and Carbohydrate Synthesis in Plants UPDATED Molecular Structure Tutorial: Bacteriorhodopsin UPDATED Animated Mechanism Figure: Rubisco Mechanism Chapter 22 Biosynthesis of Amino Acids, Nucleotides, and Related Molecules UPDATED Animated Mechanism Figures: Tryptophan Synthase Mechanism Thymidylate Synthase Mechanism Chapter 23 Hormonal Regulation and Integration of Mammalian Metabolism NEW Case Study: A Runner’s Experiment—Integration of Metabolism (Chs 14–18) Chapter 24 Genes and Chromosomes Animation: Three-Dimensional Packaging of Nuclear Chromosomes Chapter 25 DNA Metabolism UPDATED Molecular Structure Tutorial: Restriction Endonucleases NEW Simulations: DNA Replication DNA Polymerase Mutation and Repair NEW Nature Article with Assessment: Looking at DNA Polymerase III Up Close Animations: Nucleotide Polymerization by DNA Polymerase DNA Synthesis Chapter 26 RNA Metabolism UPDATED Molecular Structure Tutorial: Hammerhead Ribozyme NEW Simulations: Transcription mRNA Processing NEW Animated Mechanism Figure: RNA Polymerase NEW Nature Article with Assessment: Alternative RNA Cleavage and Polyadenylation Animations: mRNA Splicing Life Cycle of an mRNA Chapter 27 Protein Metabolism NEW Simulation: Translation NEW Nature Article with Assessment: Expanding the Genetic Code in the Laboratory Chapter 28 Regulation of Gene Expression UPDATED Molecular Structure Tutorial: Lac Repressor Lehninger Principles of Biochemistry Lehninger Principles of Biochemistry SEVENTH EDITION David L. Nelson Professor Emeritus of Biochemistry University of Wisconsin–Madison Michael M. Cox Professor of Biochemistry University of Wisconsin–Madison