Journal of Microscopy 1992. Vol. 166 Contents and Indices Joint Editors: P. Echlin, K. M. Knowles, T. Mattfeldt, J. B. Pawley, D. B. Williams EDITORIAL ADVISORY BOARD K. Tanaka, Japan M. J. Goringe, U.K. *H. J. Tanke, C. Hammond, U.K. . Long, U.K. The Netherlands . Loretto, U.K. B. F. Trump, U.S.A. B. Afzelius, Sweden . Lorimer, U.K. E. R. Weibel, *M. A. Browne, U.K. . Newbury, U.S.A. Switzerland A. Boyde, U.K. 3. Morgan, U.K. M. A. Williams, U.K. S. Bradbury, U.K. . F. Page, U.K. *T. Wilson, U.K. G. J. Brakenhoff, M. Petran, The Netherlands Czechoslovakia Representatives for the Gillian R. Bullock, H. Pillar, Germany International Society Switzerland M. Pluta, Poland for Stereology K. Carlsson, Sweden B. Ralph, U.K. J.-L. Chermant, France J. N. Chapman, U.K. L. Reimer, Germany A. J. Baddeley, A. V. Crewe, U.S.A. F. W. D. Rost, The Netherlands A. G. Cullis, U.K. Australia *L. M. Cruz-Orive, *Audrey M. Glauert, U.K. J. D. Shelburne, U.S.A. Switzerland C. Fiori, U.S.A. *Ryuichi Shimizu, Japan H. J. G. Gundersen, R. M. Glaeser, U.S.A. U. B. Sleytr, Austria Denmark D. T. Grubb, U.S.A. *D. J. Smith, U.S.A. C. Lantuéjoul, France *C. V. Howard (I.S.S. *W. M. Stobbs, U.K. R. E. Miles, Australia President), U.K. T. Takahashi, Japan *J. P. Rigaut, France A. Howie, U.K. *Associate Editor Executive Editor: GILLIAN WILSON Published for the Royal Microscopical Society by Blackwell Scientific Publications @/ Oxford London Edinburgh Boston Melbourne Paris Berlin Vienna Journal of Microscopy Patron: HER MAJESTY THE QUEEN Officers and Council 1992-93 President: M. J. GORINGE Vice-President: S. BRADBURY Vice-President and International Secretary: H. Y. ELDER Executive Hon. Secretary: C. HAMMOND Hon. Treasurer: C. V. HOWARD Hon. Secretary (Education): N. G. WRIGLEY Hon. Secretary (Science): C. J. F. vaN NOORDEN Ordinary Members of Council: D. E. ASHHURST P. J. GOODHEW G. R. COULTON C. HAWES P. CRAWLEY R. MACKENZIE A. G. CULLIS M. G. ORMEROD P. ECHLIN T. F. PAGE Hon. Joint Editors: P. ECHLIN J. B. PAWLEY K. M. KNOWLES D. B. WILLIAMS T. MATTFELDT Executive Editor: GILLIAN M. M. WILSON Administrator: P. B. HIRST Copyright. Papers accepted become the copyright of the journal (© 1992 The Royal Microscopical Society). Authorization to photocopy for internal or personal use or the internal or personal use of specific clients is granted by The Royal Microscopical Society for libraries and other users registered with the Copyright Clearance Centre (CCC) Transactional Reporting Service, provided that the stated base fee of $6.00 per copy is paid directly to the CCC, 27 Congress Street, Salem, MA 01970, U.S.A. This consent does not extend to other kinds of copying, such as copying for general distribution, for advertising or promotional purposes, for creating new collective works, or for resale. Special requests should be addressed to the Editor. 0022-2720/92 $6.00. Contents of Vol. 166 Part 1 Introduction N. Harris. Molecular histochemistry and plant biology: a review KarL J. OPARKA and Curis HAwEs. Vacuolar sequestration of fluorescent probes in plant cells: a review M. D. FRICKER and N. S. WHITE. Wavelength considerations in confocal microscopy of botanical specimens M. MICHEL, H. GNAGI and M. MULLER. Diamonds are a cryosectioner’s best friend N. D. Reap, W. T. G. ALLAN, H. KNIGHT, M. R. KNIGHT, R. MALHO, A. RUSSELL, P. S. SHACKLOCK and A. J. TREWAVAS. Imaging and measurement of cytosolic free calcium in plant and fungal cells PETER SHAW, MaRTIN HIGHETT and DaviD RAWLINS. Confocal microscopy and image processing in the study of plant nuclear structure CLIVE W. LLoyD, CATHERINA J. VENVERLOO, Kim C. GOODBODY and PETER J. SHAW. Confocal laser microscopy and three-dimensional reconstruction of nucleus-associated microtubules in the division plane of vacuolated plant cells B. Vian, M. TEMSAH, D. REIS and J. C. ROLAND. Co-localization of the cellulose framework and cell-wall matrix in helicoidal constructions MAuREEN C. MCCANN, BRIAN WELLS and KEITH ROBERTS. Complexity in the spatial localization and length distribution of plant cell-wall matrix polysacchar- ides G. I. McFappeEn, M. A. ANDERSON, I. BONIG, J. E. Gray and A. E. CLARKE. Self- incompatibility: insights through microscopy Book Reviews Rapid Publications P. S. TURNER and T. J. WHITE. On the determination of precise k-factors for AEM RICHARD HALLGREN and CLAUS BUCHHOLZ. Improved solid surface rendering with the simulated fluorescence process (SFP) algorithm Part 2 THORSTEN SCHORMANN and THOMAS M. JovIn. Contrast enhancement and depth perception in three-dimensional representations of differential interference contrast and confocal scanning laser microscope images V. Conan, S. GESBERT, C. V. HOWARD, D. JEULIN, F. MEYER and D. RENARD. Geostatistical and morphological methods applied to three-dimensional micro- scopy D. J. GOLDSTEIN. Resolution in light microscopy studied by computer simulation RICHARD W. Horosin, PETER O. GERRITS and DaAvip J. WRIGHT. Staining sections of water-miscible resins. 2. Effects of staining-reagent lipophilicity on the staining of glycol-methacrylate-embedded tissues WILLIAM B. GREENE and LyLe G. WALSH. An improved cryo-jet freezing method GILLES HEJBLUM, JEAN-YVES MARY, VINCENT MORICE and ANDRE AURENGO. Interpretation of electron microscope autoradiographs: correction for cross-fire when membrane components are highly labelled M. DiocraiuTi, P. Picozz1, S. SaNTuccI, L. Lozzi and M. DE CRESCENZI. Extended electron energy-loss fine structure and selected-area electron diffrac- tion studies of small palladium clusters BRITTA HOFFMANN-MILLACK, CLIVE J. ROBERTS and WILLIAM S. STEER. Observation of rhombic facets on platinum wire after heating in air using scanning tunnelling microscopy Letters to the Editor Erratum Rapid Publication BILLY W. Loo, JR, SHAWN WILLIAMS, STANLEY MEIZEL and STEPHEN S. ROTHMAN. X-ray stereomicroscopy: high resolution 3-D imaging of human spermatozoa in aqueous suspension with natural contrast Part 3 Editorial I. FROMM, L. REIMER and R. RENNEKAMP. Investigation and use of plasmon losses in energy-filtering transmission electron microscopy J. VAN PUYMBROECK, W. JAcoB and P. VAN EsPEN. Methodology for spectrum evaluation in quantitative electron energy-loss spectrometry using the Zeiss CEM902 E. S. GELsEMA, A. L. D. BECKERS, C. W. J. SORBER and W. C. De Bruijn. Quantification procedures for electron energy-loss spectroscopy and imaging: the use of correspondence analysis for element determination H. TENAILLEAU and J. M. Martin. A new background subtraction for low- energy EELS core edges PATRICK BERGHMANS, WIM JACOB and FREDDY ADAMS. Ultrastructural localiza- tion of titanium in chemically modified chrysotile asbestos D. M. Huonc, M. DRECHSLER, M. MOLLER and H.-J. CaNtow. Electron spectroscopic imaging of the morphology of in situ polyethylene ‘shish kebabs’ U. PLaTE, H. J. HOHLING, L. REIMER, R. H. BARCKHAUuS, R. WEINECKE, H. P. WIESMANN and A. BoybE. Analysis of the calcium distribution in predentine by EELS and of the early crystal formation in dentine by ESI and ESD KARL-HEINZ KOrTJE and Doris KOrtTyjE. The application of electron spectro- scopic imaging for quantification of the area fractions of calcium-containing precipitates in nervous tissue U.-R. HEINRICH, J. MAURER, W. MANN and W. Kreutz. Localization of Ca?*- stores and tissue compartments with a Ca’*-binding capacity in the organ of Corti of the guinea-pig by electron energy-loss spectroscopy I. M. HuxHaM, M. N. Gaze, P. WORKMAN and R. J. Marrs. The use of parallel EEL spectral imaging and elemental mapping in the rapid assessment of anti- cancer drug localization BRIAN P. MITCHELSON. The electron energy-loss spectroscopic analysis of inhaled smoke particles R. R. SCHRODER. Zero-loss energy-filtered imaging of frozen-hydrated proteins: model calculations and implications for future developments H. FEHRENBACH, J. RICHTER and Pu. A. SCHNABEL. Electron spectroscopic study (ESI, EELS) of Nanoplast-embedded mammalian lung Letter to the Editor Book Review Index of key words Aequorin, 57 Electron diffraction, 389 Affinity detection, 111 Electron energy-loss spectroscopy, 257, 297, 329, Analytical electron microscopy, 207 359 Anionic charges, 111 Electron energy-loss spectroscopy (EELS), 401 Artefact, 199 Electron microscope autoradiography, 219 Electron microscopy, 123, 343, 381 Background subtraction, 297 Electron scattering, 389 Biological material, 389 Electron spectroscopic diffraction, 329 Boron, 367 Electron spectroscopic imaging, 287, 307, 329, 343 Boron carrier, 367 Electron spectroscopic imaging and diffraction, Brain, 343 257 Electron spectroscopic imaging (ESI), 401 Calcium, 57, 343 Element distribution image, 287 Calcium-binding capacity, 359 Elemental analysis, 381 Calcium gradient, 329 Elemental mapping, 367 Calcium Green, 57 Endocytosis, 15 Calcium localization, 359 Energy filter, 43 Calcium phosphate formation, 329 Energy-filtered transmission electron microscopy Cancer therapy, 367 (EFTEM), 389 Cell-wall architecture, 123 Entropy, 155 Cellulose, 111 ESI, 317, 343 Chromatic abberation, 29 Expectation-maximization algorithm, 219 Chromatin, 401 Extended electron energy-loss fine structure, 231 Chromosomes, 29 Clusters, 231 Fast freeze/deep-etch, 123 Coherence, 185 Fluo-3, 57 Computer, 185 Fluorescein isothiocyanate, 15 Condenser, 185 Fluorescence, 185 Confocal microscopy, 57, 87 Fluorescence microscopy, 155 Confocal ratio imaging, 29 Fluorescence ratio imaging, 57 Confocal scanning, 185 Fluorescent probes, 15 Confocal scanning laser microscopy, 99 Fourier, 185 Contrast enhancement, 389 Freeze substitution, 207 Correspondence analysis, 287 Cross-fire, 219 Cryo-jet freezing, 207 Gene expression, 3 Cryo-stage, 307 Geostatistics, 169 Cryobiology, 207 Glycol methacrylate (GMA), 199 Cryogens, 207 Guard cells, 29 Guinea-pig, 359 Cryosectioning, 43 Cryotechniques, 207 Crystalline morphology, 317 HeCd laser, 29 CSLM, 155 Helicoidal cell wall, 111 Cutting artefacts, 43 High-pressure freezing, 43 Cytochemistry, 343 Histochemistry, 3 Holey carbon film, 307 Dark-field imaging, 317 Homogalacturonans, 111 Dentine, 329 Detection limits, 273 Ice, 389 Development, 3 Image processing, 87, 169 Diamond knives, 43 Image reconstruction, 99 Differential interference contrast, 155 Immunocytochemistry, 3, 137 Dispersion, 257 Immunogold labelling, 123 Division plane, 99 In situ hybridization, 3, 87, 137 Drug localization, 367 Indo-1, 57 Inhalation, 381 EELS, 307 Inner ear, 359 EFEM, 317 Inverse power law, 273 EFTEM, 317 Iodine, 367 Elastic-inelastic cross-section, 389 Ionization electrode, 43 Linear filters, 169 Radiopharmaceutical, 367 Linear regression, 273 rDNA genes, 87 Lipophilicity, 199 Regulation, 3 Low energy losses, 297 Resolution, 185, 219 Low-temperature microscopy, 43 Retina, 343 Lowicryl, 317 Rotary-shadowed replicas, 123 Lucifer Yellow, 15 Lung parenchyma, 401 S-RNases, 137 Scanning tunnelling microscopy, 247 Mathematical morphology, 169 Scattering cross-sections, 389 Measurement of mass thickness by electron Selected-area electron diffraction, 231 transmission, 329 Self-incompatibility, 137 Medium-thick specimens, 297 Sequestration, 15 Membrane labelling, 219 ‘Shish kebabs’, 317 mIBG, 367 Signal transduction, 57 Microcrystals, 389 Silicon oxide, 297 Molecular biology, 3 Simulated annealing, 219 Multiple-wavelength confocal microscopy, 29 Simulation, 185 Simulations, 219 Nanoplast embedding, 401 Smoke, 381 Spinal cord explants, 207 New three-parameter function, 297 Stereo reconstruction, 155 Nicotiana alata, 137 Non-uniform variance, 273 Stereology, 169 Structure-activity relationship, 199 Nuclear structure, 87 Structure-staining correlations, 199 Numerical aperture, 185 Subtractive cytochemistry, 111 Super-resolution, 185 Objective, 185 Synapse, 343 Obliquity, 185 Organ of Corti, 359 Tannic acid, 401 Teleost, 343 Palladium, 231 Telomeres, 87 Parallel EEL spectra, 367 Thermal faceting, 247 Particles, 381 Thin fibre section, 307 Patterns, 3 Three-dimensional deconvolution, 87 Pectin, 123 Three-dimensional digitization, 169 Phosphorus distribution, 401 Three-dimensional measurements, 169 Photometry, 57 Three-dimensional optical microscopy, 87 Plant, 3 Tissue culture, 207 Plant cells, 15 Titanium-trichloride-treated chrysotile asbestos, Plant leaves, 43 307 Plasmon losses, 257 Pollination, 137 Ultraviolet, 29 Polycrystalline platinum, 247 Universal point curve, 219 Polystyrene, 367 Polyethylene, 317 Variance functions, 273 Pre-prophase band, 99 Vitrified proteins, 389 Predentine, 329 Predentine-dentine border, 329 Wallis transformation, 155 Protein structure determination, 389 Water-miscible resin, 199 Quantification, 287, 343 Xyloglucan, 111, 123 Quantitative analysis, 297 Quantitative EELS, 273 Zero-loss energy filtering, 389 Index of authors Adams, F., 307 Mann, W., 359 Allan, W.T.G., 57 Martin, J.M., 297 Anderson, M.S., 137 Mary, J-Y., 219 Aurengo, A., 219 Maurer, J., 359 Barckhaus, R.H., 329 Meizel, S., RP5 Beckers, A.L.D., 287 Meyer, F., 169 Berghmans, P., 307 Michel, M., 43 Bonig, I., 137 Mitchelson, B.P., 381 Boyde, A., 329 Moller, M., 317 Buchholz, C., RP3 Morice, V., 219 Miller, M., 43 Cantow, H.-J., 317 Clarke, A.E., 137 Ollacarizquetta, M.A., 253 Conan, V., 169 Olmedilla, A., 253 Oparka, K.J., 15 De Bruijn, W.C., 287 Picozzi, P., 231 De Crescenzi, M., 231 Plate, U., 329 Diociaiuti, M., 231 Drechsler, M., 317 Rawlins, D., 87 Read, N.D., 57 Fehrenbach, H., 401 Reimer, L., 257, 329 Fricker, M.D., 29 Reis, D., 111 Fromm, I., 257 Renard, D., 169 Gaze, M.N., 367 Rennekamp, R., 257 Gelsema, E.S., 287 Richter, J., 401 Gerrits, P.O., 199 Risuefio, M.C., 253 Gesbert, S., 169 Roberts, C.J., 247 Gnagi, H., 43 Roberts, K., 123 Goldstein, D.J., 185 Roland, J.C., 111 Goodbody, K.C., 99 Rothman, S.S., RP5 Gray, J.E., 137 Russell, A., 57 Greene, W.B., 207 Sanchez-Pina, M.A., 253 Gunning, B., 1 Santucci, S., 231 Hallgren, R.C., RP3 Schnabel, Ph.A., 401 Harris, N., 3 Schormann, T., 155 Hawes, C., 15 Schréder, R.R., 389 Heinrich, U.-R., 359 Shacklock, P.S., 57 Hejblum, G., 219 Shaw, P., 87, 99 Highett, M., 87 Sorber, C.W.J., 287 Hoffmann-Millack, B., 247 Steer, W.S., 247 Hohling, H.J., 329 Stockert, J.C., 253 Horobin, R.W., 199 Tandler, C.J., 253 Howard, C.V., 169 Temsah, M., 111 Huong, D.M., 317 Tenailleau, H., 297 Huxham, I.M., 367 Testillano, P.S., 253 Jacob, W., 273, 307 Trewavas, A.J., 57 Jeulin, D., 169 Turner, P.S., RP1 Jovin, T.M., 155 Van Espen, P., 273 Knight, H., 57 Van Puymbroeck, J., 273 Knight, M.R., 57 Venverloo, C.J., 99 K6rtje, D., 343 Vian, B., 111 KG6rtje, K-H., 343 Villiger, W., 417 Kreutz, W., 359 Walsh, L.G., 207 Lloyd, C.W., 99 Wells, B., 123 Loo, B.W., Jr, RP5 White, N.S., 29 Lozzi,'L., 231 White, T.J., RP1 Wienecke, R., 329 McCann, M.C., 123 Wiesmann, H.P., 329 McFadden, G.I., 137 Williams, S., RP5 Mairs, R.J., 367 Workman, P., 367 Malho, R., 57 Wright, D.J., 199