Table Of Content

Journal of Immunological Methods, 141 (1991) 297-298 © 1991 Elsevier Science Publishers B.V. 0022-1759/91/$03.50 Contents to volume 141 (1991) Number 1 - 26 July 1991 Review article Assays for transforming growth factor B A. Meager (U.K.) Research reports Cell mediated cytotoxicity against U 937 cells by human monocytes and macrophages in a modified colorimetric MTT assay. A methodological study A.A. Van de Loosdrecht, E. Nennie, G.J. Ossenkoppele, R.H.J. Beelen and M.M.A.C. Langenhuijsen (The Netherlands) A versatile dot-ELISA method with femtomole sensitivity for detecting small peptides P. Sithigorngul, A.O.W. Stretton and C. Cowden (U.S.A.) Methods for the detection of anti-endothelial antibodies by enzyme-linked immunosorbent assay A.H.M. Heurkens, A. Gorter, T.M. De Vreede, C.S. Edgell, F.C. Breedveld and M.R. Daha (The Netherlands, U.S.A.) A visual assay for quantitating neutrophil chemotaxis in a collagen gel matrix. A novel chemotactic chamber J.L. Haddox, R.R. Pfister and CI. Sommers (U.S.A.) Efficient gene transfer and expression in primary B lymphocytes R.W. Overell, K.E. Weisser, B.W. Hess, S.F. Ziegler, C.M. Pleiman, C. Maliszewski and K.H. Grabstein (U.S.A.) A new technique, requiring small amounts of cells, for the parallel study of chemiluminescence and phagocytosis via different receptors in the same cell population C.H. Sandgren, K. Nordling and I. Bjérk (Sweden) Mannan-binding protein in human liver N.G. Ryley, A.R. Heryet, J. Lu, K.B.M. Reid and K.A. Fleming (U.K.) Production, characterisation and use of monoclonal antibodies to human interleukin-5 in an enzyme-linked immunosorbent assay L.A. McNamee, D.I. Fattah, T.J. Baker, S.K. Bains and P.H. Hissey (U.K.) Identification of epitopes recognized by a panel of six anti-human IgG2 monoclonal antibodies S. Harada, S§. Hata, Y. Kosada and E. Kondo (Japan) Eosinophil protein X/eosinophil derived neurotoxin (EPX/EDN). Detection by enzyme-linked immunosorbent assay and purification from normal human urine C.M. Reimert, U. Minuva, A. Kharazmi and K. Bendtzen (Denmark) Freezing of cells - Replacement of serum by oxypolygelatine J. Groth, J. Steinmann, V. Eckstein and W. Miiller-Ruchholtz (F.R.G.) A rapid and simple method for the preparation of a monospecific antibody to ovine haptoglobin D. Levieux and A. Venien (France) A *'Cr release cytotoxicity assay for use with human intrahepatic biliary epithelial cells R.C.S. Ayres, J. Shaw, C.O. Mills, R. Coleman and J.M. Neuberger (U.K.) Generation of anti-human CD8£-specific antibodies using transfectants expressing mixed-species CD8 heterodimers J.P. DiSanto, L.A. Terry and N. Flomenberg (U.S.A.) Production of monoclonal antibody against granulomonopoietic enhancing activity (GM-EA) C.-W. Lieu, C.-K. Ho and S.-Y. Wang (Taiwan) Technical note Protease treatment of nitrocellulose-bound antigens enhances the sensitivity of Western blots J. Pan and N. Auersperg (Canada) Announcement Notes to authors 298 Number 2 - 9 August 1991 Research reports An enzyme immunoassay for human defensins A.V. Panyutich, N.N. Voitenok, R.I. Lehrer and T. Ganz (U.S.A., U.S.S.R.) PH sensitive liposomes provide an efficient means of sensitizing target cells to class I restricted CTL recognition of a soluble protein R. Reddy, F. Zhou, L. Huang, F. Carbone, M. Bevan and B.T. Rouse (U.S.A.) Selection of hybrid hybridomas by flow cytometry using a new combination of fluorescent vital stains T. Shi, A.M. Eaton and D.B. Ring (U.S.A.) A new method of gene transfer into hematopoietic progenitors using liquid culture with interleukin-3 and interleukin-6 K. Hamada, T. Akagi, A. Okano, S. Taki, K. Shimotohno and Y. Takahara (Japan) Isolation of a 30 kDa immunoglobulin binding protein from Pseudomonas maltophilia S. Grover, Z.A. McGee and W.D. Odell (U.S.A.) Measurement of affinity of viral monoclonal antibodies by ELISA titration of free antibody in equilibrium mixtures A. Azimzadeh and M.H.V. Van Regenmortel (France) Purification and characterisation of human immunoglobulin lgAl1 and IgA2 isotypes from serum L.M. Loomes, W.W. Stewart, R.L. Mazengera, B.W. Senior and M.A. Kerr (U.K.) Isolation of human complement subcomponents Clr and Cls in their unactivated, proenzyme forms P.D. Lane, V.N. Schumaker, Y. Tseng and P.H. Poon (U.S.A.) Construction of representative immunoglobulin variable region cDNA libraries from human peripheral blood lymphocytes without in vitro stimulation C. Huang and B.D. Stollar (U.S.A.) Development of immunoassays for the detection of soluble tumour necrosis factor receptors N.-B. Liabakk, A. Sundan, A. Waage, M. Brockhaus, H. Loetcher, W. Lesslauer and T. Espevik (Norway, Switzerland) Epitope mapping employing immobilized synthetic peptides. How specific is the reactivity of these peptides with antiserum raised against the parent protein? R. Savoca, C. Schwab and H.R. Bosshard (Switzerland) An appraisal of polystyrene- (ELISA) and nitrocellulose-based (ELIFA) enzyme immunoassay systems using monoclonal antibodies reactive toward antigenically distinct forms of human C-reactive protein M.J. Shields, J.N. Siegel, C.R. Clark, K.K. Hines, L.A. Potempa, H. Gewurz and B. Anderson (U.S.A.) A sensitive and efficient induction system for murine IgE. Single cell analysis at the clonal level F. Ledermann, C. Schlienger, K. Wagner and C. Heusser (Switzerland) Sensitive immunometric assays for secretory peroxidase and myeloperoxidase in human saliva P. Vilja, M. Lumikari, J. Tenovuo, G. Sievers and P. Tuohimaa (Finland) Short communication A rapid method to determine the isotype and specificity of coproantibodies in mice infected with Trichinella or fed cholera toxin T. DeVos and T.A. Dick (Canada) Letters to the editors Evaluation of a closed perfusion chamber for single cell fluorescence measurements J.F.M. Wetzels, A. Kribben, T.J. Burke and R.W. Schrier (U.S.A.) Reply to the letter of J.F.M. Wetzels et al. C. Ince (The Netherlands) Announcements Contents to volume 14] (1991) Notes to authors