Journal of Biochemical and Biophysical Methods, 22 (1991) 343-345 © 1991 Elsevier Science Publishers B.V. 0165-/0912 /2$03X.5 0 Author Index Adams, R.L.P., Rinaldi, A. and Seivwright, C. Frank Jzn, J., see Rouwenhorst, R.J. (22) 119 Microassay for DNA methyltransferase (22) 19 Galacteros, F., see Vasseur, C. (22) 195 Akabane, Y., see Hisabori, T. (22) 253 Gaub, H.E., see Heyn, S.-P. (22) 145 Alon, R., Bayer, E.A. and Wilchek, M. Gemeiner, P., see Breier, A. (22) 185 A coupled enzyme assay for measurement of Gitlin, $.D., see Lindholm, P.F. (22) 233 sialidase activity (22) 23 Gohi, F.M., see Tribout, M. (22) 129 Anderson, M.L.M., Spandidos, D.A. and Cog- Gonzalez-Manas, J.M., see Tribout, M. (22) 129 gins, J.R. Grenier, D. Electroporation of lymphoid cells: factors af- Assay for protease inhibitors: qualitative fecting the efficiency of transfection (22) 207 measurement and detection in polyacryla- mide gel electrophoretograms (22) 35 Battistin, L., see Scarpa, M. (22) i35 Groff, P., see Vasseur, C. (22) 195 Bayer, E.A., see Alon, R. (22) 23 Guthridge, M., Bertolini, J., Schmitt, J. and Bertolini, J., see Guthridge, M. (22) 279 Hearn, M.T.W. Bracco, F., see Scarpa, M. (22) 135 Detection of FGF-8 mRNA in chondro- Brady, J.N., see Lindholm, P.F. (22) 233 sarcoma cells by a new in situ hybridization Breier, A., DuriSova, V., Stankovigova, T., Mis- technique with synthetic oligonucleotide lovi¢éova, D. and Gemeiner, P. probes (22) 279 Application of adsorption kinetics for estima- tion of dissociation constants (22) 185 Hider, D.-P. and Vogel, K. Briggner, L.-E. and Wadsé, I. Interactive image analysis system to de- Test and calibration processes for micro- termine the motility and velocity of cyano- calorimeters, with special reference to heat bacterial filaments (22) 289 conduction instruments used with aqueous Hearn, M.T.W., see Guthridge, M. (22) 279 systems (22) 101 Hendil, K.B. and Uerkvitz, W. Brolin, S.E. and Wettermark, G. The human multicatalytic proteinase: affinity Analytical applications of bioluminescence -— purification using a monoclonal antibody (22) a matter of proper kinetic design and record- 159 ing (22) 1 Hernandez, M.T., see Miré, A. (22) 177 Heyn, S.-P., Tillmann, R.W., Egger, M. and Cameselle, J.C., see Mird, A. (22) 177 Gaub, H.E. Chignell, C.F., see Li, A.S.W. (22) 83 A miniaturized micro-fluorescence film bal- Coggins, J.R., see Anderson, M.L.M. (22) 207 ance for protein-containing lipid monolayers Costas, M.J., see Miré, A. (22) 177 spread from a vesicle suspension (22) 145 Hirano, H., see Kajiwara, H. (22) 263 Daseler, W., Steinhoff, H.-J. and Redhardt, A. Hisabori, T., Inoue, K., Akabane, Y., Iwakami, A new method for the determination of the S. and Manabe, K. permittivity of small samples in the micro- Two-dimensional gel electrophoresis of the wave range and its application to hemoglobin membrane-bound protein complexes, includ- single crystals (22) 69 ing photosystem I, of thylakoid membranes DuriSova, V., see Breier, A. (22) 185 in the presence of sodium oligooxyethylene alkyl ether sulfate/dimethyl dodecylamine Egger, M., see Heyn, S.-P. (22) 145 oxide and sodium dodecyl sulfate (22) 253 344 Hjertén, S., see Li, J.-p. (22) 311 Lénnbro, P. and Wadsé, I. Holmquist, L. and Lewin, M. Effect of dimethyl sulphoxide and some anti- Separation of glutaraldehyde and some of its biotics on cultured human T-lymphoma cells aldol condensation products by hydroxyl-al- as measured by microcalorimetry (22) 331 dehyde group affinity chromatography (22) 321 Manabe, K., see Hisabori, T. (22) 253 Hwang, B.K., see Suh, J. (22) 167 Marriott, S.J., see Lindholm, P.F. (22) 233 Matsuura, S., see Kambayashi, J. (22) 93 Inoue, K., see Hisabori, T. (22) 253 Meezan, E., see Pillion, D.J. (22) 89 Iwakami, S., see Hisabori, T. (22) 253 Miré, A., Hernandez, M.T., Costas, M.J. and Cameselle, J.C. Jang, I., see Suh, J. (22) 167 Enzyme saturation and inhibition kinetics Janmey, P.A. studied from multiple progress curves re- A torsion pendulum for measurement of the corded spectrophotometrically from single re- viscoelasticity of biopolymers and its applica- action mixtures for ADP-ribose pyrophos- tion to actin networks (22) 41 phatase (22) 177 Mishra, R.K., see Ramesh, N. (22) 171 Kajiwara, H., Hirano, H. and Oono, K. Mislovicova, D., see Breier, A. (22) 185 Binding shift assay of parvalbumin, calmodu- Momo, F., see Scarpa, M. (22) 135 lin and carbonic anhydrase by high-perfor- Mori, T., see Kambayashi, J. (22) 93 mance capillary electrophoresis (22) 263 Kambayashi, J., Yokota, M., Sakon, M., Shiba, Oh, E., see Suh, J. (22) 167 E., Kawasaki, T., Mori, T., Tsuchiya, M., Oishi, H., see Kambayashi, J. (22) 93 Oishi, H. and Matsuura, S. Oono, K., see Kajiwara, H. (22) 263 A novel endotoxin-specific assay by turbidi- metry with Limulus amoebocyte lysate con- Paredes, S., see Tribout, M. (22) 129 taining B-glucan (22) 93 Parker, I., see Tigyi, G. (22) 243 Kawasaki, T., see Kambayashi, J. (22) 93 Pillion, D.J., Kim, S.-J. and Meezan, E. Kim, S.-J., see Pillion, D.J. (22) 89 Increased sensitivity of *°p and '*I counted Krull, U.J., see Vandenberg, E.T. (22) 269 in the presence of a solid scintillator: detec- tion of protein bands separated by poly- Lammi, M.J. and Tammi, M. acrylamide gel electrophoresis (22) 89 Autoradiographic quantitation of radiolabel- ed proteoglycans (22) 301 Ramesh, N. and Mishra, R.K. Lewin, M., see Holmquist, L. (22) 321 Screening of clones containing altered DNA Li, A.S.W. and Chignell, C.F. conformations by plasmid topoprofile shift The NoH value in EPR spin trapping: a new (22) 171 parameter for the identification of 5,5-di- Redhardt, A., see Daseler, W. (22) 69 methyl-1-pyrroline-N-oxide spin adducts (22) Rigo, A., see Scarpa, M. (22) 135 83 Rinaldi, A., see Adams, R.L.P. (22) 19 i, J.-p. and Hjertén, S. Rouwenhorst, R.J., Frank Jzn, J., Scheffers, W.A. High-performance liquid chromatography of and Van Dijken, J.P. proteins on deformed nonporous agarose Determination of protein concentration by beads: immuno-affinity chromatography, ex- total organic carbon analysis (22) 119 emplified with human growth hormone as ligand and a combination of ethylene glycol Safarik, I. and salt for desorption of the antibodies (22) Spectrophotometric determination of amylase 311 activity in coloured solutions (22) 61 Lin, F., see Wise, G.E. (22) 223 Sakon, M., see Kambayashi, J. (22) 93 Lindholm, P.F., Marriott, $.J., Gitlin, S.D. and Scarpa, M., Viglino, P., Momo, F., Bracco, F., Brady, J.N. Battistin, L. and Rigo, A. Differential precipitation and zinc chelate NMR method for superoxide dismutase assay chromatography purification of biologically in brain and liver homogenates (22) 135 active HTLV-I Tax, expressed in E. coli (22) Scheffers, W.A., see Rouwenhorst, R.J. (22) 119 233 345 Schmitt, J., see Guthridge, M. (22) 279 The prevention of adsorption of interferents Seivwright, C., see Adams, R.L.P. (22) 19 to radiolabelled protein by Tween 20 (22) 269 Shiba, E., see Kambayashi, J. (22) 93 Van Dijken, J.P., see Rouwenhorst, R.J. (22) 119 Spandidos, D.A., see Anderson, M.L.M. (22) 207 Vasseur, C., Galacteros, F., Groff, P. and Wajc- Stankovitova, T., see Breier, A. (22) 185 man, H. Steinhoff, H.-J., see Daseler, W. (22) 69 Cleavage by protease from Staphylococcus Suh, J., Hwang, B.K., Jang, I. and Oh, E. aureus V8: an improvement in the sequence Spectral titration of active site of carboxy- analysis of human hemoglobin variants (22) peptidase A (22) 167 195 Viglino, P., see Scarpa, M. (22) 135 Tammi, M., see Lammi, M.J. (22) 301 Vogel, K., see Hader, D.-P. (22) 289 Tigyi, G. and Parker, I. Microinjection into Xenopus oocytes: a pre- Wadsé, I., see Briggner, L.-E. (22) 101 cise semi-automatic instrument and optimal Wadsé, I., see Lénnbro, P. (22) 331 Wajcman, H., see Vasseur, C. (22) 195 parameters for injection of mRNAs (22) 243 Tillmann, R.W., see Heyn, S.-P. (22) 145 Wettermark, G., see Brolin, S.E. (22) 1 Tribout, M., Paredes, S., Gonzalez-Manas, J.M. Wilchek, M., see Alon, R. (22) 23 Wise, G.E. and Lin, F. and Goni, F.M. Transfer of silver-stained proteins from poly- Binding of Triton X-100 to bovine serum acrylamide gels to polyvinylidene difluoride albumin as studied by surface tension mea- surements (22) 129 membranes (22) 223 Tsuchiya, M., see Kambayashi, J. (22) 93 YashRoy, R.C. Protein heat denaturation and study of mem- Uerkvitz, W., see Hendil, K.B. (22) 159 brane lipid-protein interactions by spin label ESR (22) 55 Vandenberg, E.T. and Krull, U.J. Yokota, M., see Kambayashi, J. (22) 93 Journal of Biochemical and Biophysical Methods, 22 (1991) 347-348 © 1991 Elsevier Science Publishers B.V. 0165-022X/91 /$03.50 Subject Index Actin, (22) 41 Endotoxin, (22) 93 Active-site titration, (22) 167 Enzyme assay, (22) 23; (22) 61 ADP-ribose pyrophosphatase, (22) 177 Enzyme inhibition, (22) 177 Adsorption, (22) 185 Enzyme kinetics, (22) 1; (22) 177 Affinity chromatography, (22) 311 Ethylene glycol, (22) 311 Alkyl ether sulfate, (22) 253 Amoebocyte lysate, (22) 93 Fluorescence, (22) 269 Amphotericin B, (22) 331 Amylase, (22) 61 Gel, (22) 41 Anesthetic, local, (22) 185 Gel electrophoresis, (22) 223 Antibody hGH, (22) 311 Gentamicin, (22) 331 Antigenicity, (22) 223 B-Glucan, (22) 93 Autoradiography, (22) 301 Glutaraldehyde, (22) 321 Glycosaminoglycan, (22) 301 Bioluminescence analysis, (22) 1 Glycosylated proteins, (22) 119 Biomembrane, interaction with surfactant, (22) Growth factor, (22) 279 129 Biopolymer, (22) 41 Hemoglobin, (22) 69 Black drawing ink, (22) 61 High-performance capillary electrophoresis, Bovine serum albumin, (22) 129; (22) 269 HPCE, (22) 263 Brain, (22) 135 HLTV-I Tax,, (22) 233 HPLC, (22) 195 Calibration, (22) 101 Human growth hormone (hGH), (22) 311 Calmodulin, (22) 185; (22) 263 Human hemoglobin, (22) 195 Calorimeter, (22) 101 Hydrophobic interaction, (22) 311 Capillary electrophoresis, CE, (22) 263 Hydroxyl-aldehyde group, (22) 321 Carbonic anhydrase, (22) 263 Carboxypeptidase A, (22) 167 Image analysis, (22) 289; (22) 301 CAT assay, (22) 207 Immunoaffinity chromatography, (22) 159 Chondrosarcoma, (22) 279 In situ hybridization, (22) 279 Clone selection, (22) 171 Insoluble chromolytic substrate, (22) 61 Coloured solution, (22) 61 Interaction chromatography, (22) 321 Condensation product, (22) 321 Interference, (22) 269 Cross-linked starch, (22) 61 Intermediate, (22) 167 Cyanobacteria, (22) 289 Intracellular injection, (22) 243 5,5-Dimethyl-1-pyrroline-N-oxide, (22) 83 Kainate-receptor, (22) 243 Dimethyl sulphoxide, (22) 331 Dissociation constant, (22) 185 Lactate dehydrogenase, (22) 185 DNA, (22) 19 Langmuir trough, miniaturized, (22) 145 DNA structure, altered, (22) 171 Limulus, (22) 93 Lipid-protein interaction, (22) 55 Electroporation, (22) 207 Liver, (22) 135 Electrostatic interaction, (22) 311 Lymphoid cell, (22) 207 348 Membrane, (22) 55 Radioiodination, (22) 269 Membrane protein, (22) 253 Reaction vessel, (22) 101 Methylase, (22) 19 Reactive dye, (22) 185 Methyltransferase, (22) 19 Rheology, (22) 41 Microanalysis, (22) 1 mRNA, (22) 243 Microassay, (22) 19 Microcalorimetry, (22) 331 Safranin O precipitation, (22) 301 Micro-fluorescence film balance, (22) 145 Scintillator, solid, (22) 89 Motility, (22) 289 SDS-polyacrylamide gel electrophoresis, (22) 35 Multicatalytic proteinase, (22) 159 Sensitivity, *°P and '*°I, (22) 89 Separation, (22) 321 Network, (22) 41 Shear modulus, (22) 41 Neuroleptic, (22) 185 Sialidase activity, (22) 23 NMR, (22) 135 Silver staining, (22) 223 *°SO, labeling, (22) 301 Non-ionic detergent, (22) 269 Spectrophotometric assay, (22) 61 Oligomeric, (22) 321 Spectrophotometry, (22) 177 Oligonucleotide probe, (22) 279 Spin adduct, (22) 83 Oocyte, (22) 243 Spin label ESR, (22) 55 Spin trapping, (22) 83 Partial characterization, (22) 321 Streptomycin, (22) 331 Parvalbumin, (22) 263 Supercoiling, (22) 171 Penicillin, (22) 331 Superoxide dismutase, (22) 135 Permittivity, (22) 69 Surface tension, drop-weight method for measur- Phormidium uncinatum, (22) 289 ing, (22) 129 Photodynamic reaction, (22) 289 Surfactant, binding to biomolecule, (22) 129 Photosystem I, (22) 253 Planar supported membrane preparation, (22) Test process, (22) 101 145 Thylakoid membrane, (22) 253 Polymeric, (22) 321 Time-lapse video, (22) 289 Progress curve, (22) 177 Tissue homogenate, (22) 135 Prosome, (22) 159 T-lymphoma cell, (22) 331 Protease, (22) 35 TOC analysis, (22) 119 Protease inhibitor, (22) 35 Topoprofile, (22) 171 Protease V8, (22) 195 Torsion pendulum, (22) 41 Proteasome, (22) 159 Transfection, gene transfer, (22) 207 Protein, (22) 223; (22) 269 Transient assay, (22) 207 Protein band, (22) 89 Translation, in vivo, (22) 243 Protein crystal, (22) 69 Triton X-100, (22) 129 Protein denaturation, (22) 55 Turbidimetry, (22) 93 Protein determination, (22) 119 Two-dimensional gel electrophoresis, (22) 253 Protein-lipid monolayer, air/water interface, (22) 145 Velocity, (22) 289 Protein-lipid monolayer, formation, (22) 145 Viscoelasticity, (22) 41 Protein sequence, (22) 195 Proteoglycan, (22) 301 Western blot, (22) 223 Purification, (22) 233