IS 13401: Meat and Meat Products - Determination of Thiobarbituric Acid Value in Meat - Test Method

8 Pages·1992·1 MB·English

by  Bureau of Indian Standards

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“The Right to Information, The Right to Live” “Step Out From the Old to the New”
IS 13401 (1992): Meat and Meat Products - Determination of
Thiobarbituric Acid Value in Meat - Test Method [FAD 18:
Slaughter House and Meat Industry]
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Satyanarayan Gangaram Pitroda
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“Knowledge is such a treasure which cannot be stolen”

Indian Standard
MEAT AND MEAT PRODUCTS-
DETERMINA4TION OF THIOBARBITURIC
ACID VALU'E IN MEAT-TEST METHOD
CDU 637’5’543

BUREAU OF INDIAN STANDARDS
MANAK RHAVAN. 9 IIAHADUR SHAI-1. ZAFAR MARG
WEN IHLED I 10002
yluJ 1992 ecirP puorG 1
Meat yrtsudnI lanoitceS ,eettimmoC DAF 7
DROWEROF
sihT naidnI dradnatS was adopted yb the uaeruB of naidnI ,sdradnatS after the draft dezilanif yb
the Meat yrtsudnI lanoitceS eettimmoC had neeb devorppa yb the dooF dna erutlucirgA noisiviD
.licnuoC
ehT gnol storage of taem dna taem stcudorp yam dael ot elbaredisnoc evitadixo ytidicnar ni the
exposed fat. sihT noitadixo yam tluser ni niatrec elbarisednu segnahc ni the .taem ehT yramirp
stcudorp of dipil noitadixo are sedixorepordyh dna these are ylidaer desopmoced ot ecudorp suoirav
cirutibraboiht dica evitcaer secnatsbus ( SRABT ) ylralucitrap lynobrac .sdnuopmoc ehT
cirutibraboiht-2 dica noitcaer has neeb ylediw desu as na evitcejbo erusaem of evitadixo noitaroireted
gnirrucco ni taem dna taem .stcudorp
A orcim’cificeps dohtem setamitse ABT seulav ni taem selpmas yb tcerid yrtemotohportceps gnisu
xuller noitallitsid ot separate .slynobrac
roF the esoprup of gnidiced whether a ralucitrap tnemeriuqer of siht dradnats si deilpmoc ,htiw
the lanif ,eulav devresbo or ,detaluclac gnisserpxe the tluser of a test or ,sisylana llahs eb dednuor
off ni ecnadrocca htiw SI 2 : 1960 seluR‘ for gnidnuor off laciremun seulav ( revised) '. ehT
rebmun of tnacifingis deniater’secalp ni the dednuor off eulav dluohs eb the emas as that of the
deificeps eulav ni siht .dradnats
SI 13401 : 1992
Indian Standard
MEAT AND MEAT PRODUCTS -
DETERMINATION OF THIOBARBITURIC
ACID VALUE IN MEAT - TEST METHOD
1 EPOCS latot emulov of 5 .lm ddA ABT tnegaer ( 5 lm )
dna ylthgit cap the .sebut ehT lanif snoitartnecnoc
sihT naidnI dradnatS sebircserp a test dohtem of PET ni the evoba 10 lm semulov dnopserroc
for the noitanimreted of ABT eulav of .taem ot 0, 4’0, 8’0, 12’0, 16’0 dna 20’0 ’-OI elom
x
per ertil .ylevitcepser retfA hguoroht ,gnixim
2 ELPICNIRP heat the test, sebut ylsuorogiv gniliob water
iu
htab for 45 setunim dna looc ni tap water.
laminA eussi[ hcihw has neeb detabucni -iborea
enimreteD ecnabrosba of the snoitulos at 538 mn
yllac secudorp a ruoloc htiw cirutibraboiht-2
nihtiw 30 nim of ,gnilooc gnittes the knalb
dica ( ABT ). sihT ruoloc si eud ot the tluser of
( 0’0 lm PET ) ot .oreZ
a xelpmoc demrof morf noitadixo stcudorp of
detarutasnu yttaf sdnuopmoc dna cirutibraboiht-2 ehT tolp of PET noitartnecnoc ( mp ) tsniaga
.dica ehT yramirp stcudorp of dipil noitadixo ecnabrosba at 538 mn si raenil pu ot 2’0 mp PET
are sedixorepordyh dna these rednu the snoitidnoc debircsed here. ehT ralom
are readily decom-
posed ot ecudorp suoirav cirutibraboiht dica noitcnitxe tneiciffeoc of the ruoloc depoleved
evitcaer secnatsbus ( SRABT ) ylralucitrap ( ytiralom/ecnabrosba ) si 1’9 x 5OI at 538 .mn
lynobrac .sdnuopmoc ehT cirutibraboiht-2 dica
3.4 erudecorP
noitcaer has neeb ylediw desu as na evitcejbo
erusaem of evitadixo noitaroireted gnirrucco ni yleniF chop 100 g .elpmas ecalP 10 g elpmas ni
taem dna taem .stcudorp a llams reniatnoc dna freeze .yletaidemmi -htiw
tuo ,gniwaht refsnart taem noitrop ot the rednelb
3 SUTARAPPA raj htiw 35 lm of dellitsid water dna dnelb for 2
setunim or litnu the elpmas si ylenif .dedivid
3.1 noitallitsiD ylbmessA tinU
refsnarT the elpmas etanegomoh ot a tared 500
3.1.1 Blender lm dnuor mottob ksalf gniniatnoc yletamixorppa
100 gm each of ctallaglyporp dna ANDE dna add
3.1.2 Water Bath dellitsid water so that the latot thgiew of the
elpmas dna water si 105 g. hsulF the elpnlas htiw
3.1.3 Spectrophotometer
negortin dna add 95 lm of 4 M .ICH peeK the
3.1.4 Screw Cap Test Tubes with Tejlotl Litled noitallitsid rate at tuoba eno ot two drops per
Caps - 15 x 125 .mm .dnoces ehT ,llits neewteb ,selpmas si desnir
htiw lonahtem dna neht htiw dellitsid water.
3.1.5 Mtgrretic Stirrer etaregirfeR SRABT etallitsid ,thginrevo fi
3.2 stnegaeR .yrassecen

3.5 cirtcmotohportccpS noitanimreteD
3.2.1 TBA Reagent
refsnarT 5 lm of each SRABT etallitsid dna 5 lm
xiM 1’44 g of cirutibraboiht-2 dica ni 50 lm of
of ART tnegaer otni pac-wercs test ,sebut revoc
dellitsid water ni a 1171-005 cirtemulov ksall htim
ylthgit dna treat debircsed ni dradnats evruc
ddA laicalg as
vigorous stirring ( magnetic stirrer ). .noitaraperp ylsuoenatlumiS nur a hnalb 01‘ 5 lm
citeca dica litnu the ksalf si driht-owt .lluf
of dellitsid relaw dna 5 lm of ABT .tnegaer
ylsuorogiV rits the erutxim for 10 setunim or
elpmaS snoitulos htiw ecnabrosba greater naht
litnu the cirutibraboiht-2 si tsouha yletelpmoc
0 5 llahs eb detulid htiw dellitsid water or -retla
.devlossid lliF the ksall ot the kram htiw
ylevitan the sisylana llarls cb rqeated gnisu ssel
laicalg citeca .dica
SWAHT .etallitsid
3.6 noitaluclaC of SRABT eulaV
yletaruccA hgiew 0’220 g yxohtertet enaporp Express SKART cula\ as lonr~~ edylleitla~lol~;~~~ per
( E’II‘ ) dna refsnart ot a 100 cirtemulov .ksalf kg .taem fI a 5 lin oll,llitsid deniatbo
ml al~quot 01‘
etuliD ot volurnc htiw dellitsid water ot ecudorp l‘r0~ 10 g ,taem si ,desu neht SIIABT elrliiv yam
a I X OI ‘M stock .noitulos peeK the noitulos cb detaluclac t‘rom the &ililpmis :nluinro’I
rednu .noitaregirfer Prepare a 1 X 10 M“
gnikrow noitulos yb gnitulid OI im of the stock
solullon to IO0 ml.
3.3 noitaraperP of Standard Curve
ylclaruccA e(tcpip stollqila of O, 0.4, (1 8, 1.2, 1.6
gnikrow ’litT dradnats noitulos otni
and 2’0 ml c)i
screw cap test sebut dna dda water ot ekam a

1
Standard Mark
ehT esu fo eht dradnatS Mark si de~r~cvog yb eht snolslvorp fo ehr Bur~ou u/ fnd~an
Sranhrds act, 1986 dna eht seluR dna snoitalugeK edam .rednuereht ehT dradnatS Mark no
stcudorp derevoc yb na naidnI dradnatS syevnoc eht ecnarussa taht yehr evah neeb decudorp
ot ylpmoc htiw eht srnemerluqer fo taht d~radnats rednu a llew denifed metsys fo ,noitcepsni
gnitset dna ytilauq lorlnrtC hcihw SI desived dna desivrepus yb SIH dna detarepo yb eht
.recudorp dradnatS dekram srcudorp era osla cor~t~r~~~o~~sly dekcehc yb SISI rof ytimrofnoc
ot taht dradnats sa a rehtruf .draugefas sllaWI fo snoitldmcc rednu hcihw a ecnecil rof eht
esu fo eht dradnatS Mark yam eb detnarg ot srerutcafunam ro srecudorp yam be obtained
mo-rf the Hurcau 0C IIldiarl .sdradnatS
Bureau of Indian Standards
SIB si a yrotutats noitutitsni dehsilbatse rrdnn the Brrrecrrc of Idim S~urrthrtf~A uf, 1986 to promote
harmonious tnempoleved of the seittvttca of .1i0ifl:7idradliats gnikram and ytilanq nortacititrec of goods
dna gnidnetta ot detcennoc srettam ni the .yrtnuoc
Copyright
SIB sah the thgirypoc of lla sti .snottacrlbup No part of these snortacilbnp yam eb dccndorper nr
yna mrof tuohtiw the roirp noissimrep ni gnitirw of 111s. sihT does ton edulcerp the free ,esir ni the
esrnoc of gnitnemelpmi the ,d-ladnats of yrassecen ,sliated hcus as slobmys dna ,selis epyt ro edalg
.snoitangised seiriuqnE gnitaler ot thgirypoc he addressed ot the rotceriD ( snoitacilbuP ), .SIB
Revision of Indian Standards
naidnI sdradnatS are deweiver yllactdotrep dna .deslwr llellw yrassecen dna ,stnemdnema fi any, era
deussi morf emit ot .emit sresU of natdnl sdradnatS dIrtohs niatrecsa that yctlt are ni noissecsop of
the tsetal stnemdnema or nottide stnemmoC no siht naidnI dradnatS yam eb tnes ot SIB gnivig the
gniwollof ecnerefer :
Dot : No. DAF 7 ( 21 )
stnemdnemA Issued Since noitacilbuP
dnemA No. Date of eussI txeT detceffA
BUREAU OF NAIDNI SDRADNATS
:sretrauqdaeH
kanaM ,navahB 9 rudarlaB Shah rafaZ Marg. New ihleD 110002

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