In Vitro Differentiation of T-Cells: Methods and Protocols

265 Pages·2019·8.114 MB·English

by  Shin Kaneko

Methods in
Molecular Biology 2048
Shin Kaneko Editor
In Vitro
Diff erentiation
of T-Cells
Methods and Protocols
M M B
ETHODS IN OLECULAR IO LO GY
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School of Lifeand MedicalSciences
University ofHertfordshire
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In Vitro Differentiation of T-Cells
Methods and Protocols
Edited by
Shin Kaneko
Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research
and Application, Kyoto University, Kyoto, Japan
Editor
ShinKaneko
ShinKanekoLaboratory,Department
ofCellGrowthandDifferentiation
CenterforiPSCellResearchandApplication,KyotoUniversity
Kyoto,Japan
ISSN1064-3745 ISSN1940-6029 (electronic)
MethodsinMolecularBiology
ISBN978-1-4939-9727-5 ISBN978-1-4939-9728-2 (eBook)
https://doi.org/10.1007/978-1-4939-9728-2
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Preface
The dream of in vitro replication of cell differentiation occurring within our bodies has
becomearealitywiththeemergenceofpluripotentstemcells,whichhavepavedthewayto
clinicalapplicationwiththeadventofinducedpluripotentstem(iPS)cells.Onthisoccasion,
I am honored to be able to publish this protocol book, In Vitro Differentiation of T-Cells:
MethodsandProtocols.
T-cells play a pivotal role in host defense from pathogens; however, sometimes, they
causeaseriousdisease.TheinvitroT-celldifferentiationsystemisofvitalimportancetothe
pathologicalanalysis,drugdevelopment,andcelltherapyofhumanT-cells.
In this protocol book, T-cell induction methods from human pluripotent stem cells,
suchashumanembryonicstemcells(Chapter6)andhumaniPScells(Chapters6–10),are
mainly compiled. Among the various reported methods, this book cites in vitro T-cell
differentiation protocols through co-culture with murine stromal cells (Chapter 7), feeder
cell-free condition (Chapter 8), T-cell receptor gene-transduced iPS cells (Chapter 9), and
chimericantigenreceptorgene-transducediPScells(Chapter10).Italsocontainsaprotocol
for establishing T-cell-derived iPSCs, which offers an extremely useful tool for research on
invitroT-celldifferentiationinduction(Chapter5).Furthermore,thisbookalsobriefsthe
readers on a protocol for in vitro differentiation for T-cell-related immune cells, such as
human NK cells (Chapter 12) and human NKTcells (Chapter 13). They both are near to
clinicalapplicationandareconsideredahelpfulreferenceforclinicaldevelopment.
In recent years, when medical practice of using peripheral blood T-cells as drugs has
becomearealityintheformofCARTandTCR-Tcells,wecannotdismissthepossibilityof
clinicalapplicationsofinvitrodifferentiatedT-cells.Inadditiontotheprotocolsforinvitro
cell differentiation, this book also compiles useful techniques to develop and evaluate
cellularmedicines.Specifically,thisbookcontainsthedetailsoninvitroT-celldifferentiation
frompluripotentstemcellsofmouse(Chapter14)andmonkey(Chapter11)thatareuseful
in basic research for predicting their effectiveness and safety in vivo. Furthermore, to assist
the characterization of in vitro and in vivo differentiated T-cells, the following protocols
havebeenincluded:thebasicsofflowcytometryanalysis(Chapters1and2),genetransfer
to peripheral blood T-cells and effector function analysis (Chapter 3), single T-cell tran-
scriptomeanalysis(Chapter16),invivoresearchonbloodimmunecellsusingimmunode-
ficient mice (Chapter 19), and in vivo assays for effectiveness and safety of iPSC-derived
T-cellsthroughsuicidegeneswitch(Chapter20).
In addition, this volume collects recently reported interesting technologies, including
“rejuvenating” technology for human peripheral T-cells without pluripotent stem cell
technology(Chapter4);characterizationtechniquesforthymicepithelialstemcellslocated
inthethymus,whichisthemainfieldofT-celldifferentiation(Chapter15);andmethodsfor
thestructuralanalysisofantigensandtheirspecificT-cellreceptors(Chapter17).
v
vi Preface
I would like to extend my cordial gratitude to the authors and the editors who
contributed to the creation of this protocol book despite the fact that my inexperience as
aneditorcausedthemsomuchtrouble.
Itismysincerewishthatthisbookwouldbeofhelptotheresearcherswhoareworking
onthedifferentiationofT-cellsfromstemcells.
Kyoto,Japan ShinKaneko
Contents
Preface ..................................................................... v
Contributors................................................................. ix
1 FlowCytometryAnalysistoIdentifyHumanCD8+TCells.................. 1
JacquelineFlynnandPaulGorry
2 FlowCytometryAnalysistoIdentifyHumanCD4+TCellSubsets............ 15
JacquelineFlynnandPaulGorry
3 GeneModificationandImmunologicalAnalysesfor theDevelopment
ofImmunotherapyUtilizingTCellsRedirectedwithAntigen-Specific
Receptors.............................................................. 27
ToshikiOchi,MasakiMaruta,andNaotoHirano
4 InVitroConversionofActivatedTCellsintoStemCell
Memory-LikeTCells.................................................... 41
TaisukeKondo,YukiImura,MakotoAndo,ShunsukeChikuma,
andAkihikoYoshimura
5 HumaniPSCGenerationfromAntigen-SpecificTCells ..................... 53
ToshinobuNishimura,YukikoMurmann,andHiromitsuNakauchi
6 InVitroDifferentiationofTCells:FromHumanEmbryonicStemCells
andInducedPluripotentStemCells....................................... 59
ShoichiIriguchiandShinKaneko
7 RedifferentiationofAdaptiveNa¨ıve-LikeCTLfromT-Cell-DerivediPSC...... 71
YoheiKawaiandShinKaneko
8 InVitroDifferentiationofTCell:FromHumaniPSCellsinFeeder-Free
Condition.............................................................. 77
YutakaYasui,YasumichiHitoshi,andShinKaneko
9 DifferentiatingCD8αβTCellsfromTCR-TransducediPSCsforCancer
Immunotherapy ........................................................ 81
AtsutakaMinagawaandShinKaneko
10 InVitroDifferentiationofTCell:FromCAR-ModifiedT-iPSC.............. 85
TatsukiUedaandShinKaneko
11 InVitroDifferentiationofTCells:FromNonhumanPrimate-Induced
PluripotentStemCells................................................... 93
ShoichiIriguchiandShinKaneko
12 AnImprovedMethodtoProduceClinical-ScaleNaturalKillerCells
fromHumanPluripotentStemCells ...................................... 107
HuangZhuandDanS.Kaufman
13 InVitroDetectionofCellularAdjuvantPropertiesofHumanInvariant
NaturalKillerTCells.................................................... 121
RongZhang,ShuichiKitayama,TianyiLiu,Norihiro Ueda,Yumi
Tokumitsu,HiroakiMashima,HidekiOhdan,ShinKaneko,andYasushi
Uemura
vii
viii Contents
14 InVitroDifferentiationofTCellsfromMurinePluripotent
StemCells ............................................................. 131
XiaoniaoChen,FengyangLei,LiqiangWang,XiaofangXiong,
andJianxunSong
15 ClonogenicCultureofMouseThymicEpithelialCells ...................... 143
MihoSekai,JianweiWang,andYokoHamazaki
16 Single-CellTranscriptomeAnalysisofTCells .............................. 155
WillemVanDerByl,SimoneRizzetto,JeromeSamir,CurtisCai,
AudaA.Eltahla,andFabioLuciani
17 StructuralModelingofLymphocyteReceptorsandTheirAntigens ........... 207
SonglingLi,JanWilamowski,ShunsukeTeraguchi,FlorisJ.vanEerden,
JohnRozewicki,AnaDavila,ZichangXu,KazutakaKatoh,
andDaronM.Standley
18 AssessingTLymphocyteAgingUsingTelomereLength
andTelomeraseActivityMeasurementsinLowCellNumbers................ 231
TinhinaneFali,CamilleK’Ros,VictorAppay,andDelphineSauce
19 GenerationofHematopoieticStemandProgenitorCellsfromHuman
PluripotentStemCells................................................... 245
HideyukiOguro
20 UsingtheInducibleCaspase-9Suicide-SafeguardSystemwithiPSC
andBioluminescentTracking............................................. 259
JonathanVillanueva,ToshinobuNishimura,
andHiromitsuNakauchi
Index ...................................................................... 265
Contributors
MAKOTO ANDO (cid:1) DepartmentofMicrobiologyandImmunology,KeioUniversitySchoolof
Medicine,Shinjuku-ku,Tokyo,Japan
VICTOR APPAY (cid:1) Centred’ImmunologieetdesMaladiesInfectieuses(CIMI-Paris),Sorbonne
Universite´,INSERM,Paris,France;InternationalResearchCenterofMedicalSciences
(IRCMS),KumamotoUniversity,Kumamoto,Japan
CURTISCAI (cid:1) KirbyInstituteforInfectionandImmunity,TheUniversityofNewSouth
Wales,Sydney,NSW,Australia;SchoolofMedicalSciences,TheUniversityofNewSouth
Wales,Sydney,NSW,Australia
XIAONIAOCHEN (cid:1) DepartmentofOphthalmology,ChinesePLAGeneralHospital,Beijing,
China;DepartmentofOphthalmology,HarvardMedicalSchool,Boston,MA,USA
SHUNSUKECHIKUMA (cid:1) DepartmentofMicrobiologyandImmunology,KeioUniversitySchool
ofMedicine,Shinjuku-ku,Tokyo,Japan
ANADAVILA (cid:1) GraduateSchoolofMedicine,OsakaUniversity,Osaka,Japan
AUDAA.ELTAHLA (cid:1) KirbyInstituteforInfectionandImmunity,TheUniversityofNewSouth
Wales,Sydney,NSW,Australia;SchoolofMedicalSciences,TheUniversityofNewSouth
Wales,Sydney,NSW,Australia
TINHINANEFALI (cid:1) Centred’ImmunologieetdesMaladiesInfectieuses(CIMI-Paris),
SorbonneUniversite´,INSERM,Paris,France
JACQUELINEFLYNN (cid:1) RheumatologyResearchGroup,CentreforInflammatoryDiseases,
SchoolofClinicalSciencesatMonashHealth,MonashUniversity,Melbourne,VIC,
Australia;SchoolofHealthandBiomedicalSciences,RMITUniversity,Melbourne,VIC,
Australia;BurnetInstitute,Melbourne,VIC,Australia
PAULGORRY (cid:1) SchoolofHealthandBiomedicalSciences,RMITUniversity,Melbourne,VIC,
Australia
YOKOHAMAZAKI (cid:1) DepartmentofImmunologyandCellBiology,GraduateSchoolof
Medicine,KyotoUniversity,Kyoto,Japan;LaboratoryofImmunobiology,GraduateSchool
ofMedicine,Center foriPSCellResearchandApplication(CiRA),KyotoUniversity,
Kyoto,Japan
NAOTOHIRANO (cid:1) TumorImmunotherapyProgram,PrincessMargaretCancerCentre,
UniversityHealthNetwork,Toronto,ON,Canada
YASUMICHIHITOSHI (cid:1) ThyasCo.Ltd.,Kyoto,Japan
YUKIIMURA (cid:1) DepartmentofMicrobiologyandImmunology,KeioUniversitySchoolof
Medicine,Shinjuku-ku,Tokyo,Japan;Sohyaku,InnovativeResearchDivision,Mitsubishi
TanabePharmaCorporation,Aoba-ku,Yokohama,Kanagawa,Japan
SHOICHIIRIGUCHI (cid:1) ShinKanekoLaboratory,DepartmentofCellGrowthand
Differentiation,Center foriPSResearchandApplication,KyotoUniversity,Kyoto,Japan
SHINKANEKO (cid:1) ShinKanekoLaboratory,DepartmentofCellGrowthandDifferentiation,
Center foriPSCellResearchandApplication(CiRA),KyotoUniversity,Kyoto,Japan
KAZUTAKA KATOH (cid:1) ResearchInstituteforMicrobialDiseases,OsakaUniversity,Osaka,
Japan;ImmunologyFrontierResearchCenter,OsakaUniversity,Osaka,Japan
DANS.KAUFMAN (cid:1) DivisionofRegenerativeMedicine,DepartmentofMedicine,University
ofCalifornia,SanDiego,SanDiego,CA,USA
ix