Pankaj Suman Pranjal Chandra Editors Immunodiagnostic Technologies from Laboratory to Point-Of-Care Testing Immunodiagnostic Technologies from Laboratory to Point-Of-Care Testing (cid:129) Pankaj Suman Pranjal Chandra Editors Immunodiagnostic Technologies from Laboratory to Point-Of-Care Testing Editors PankajSuman PranjalChandra AnimalBiotechnologyLaboratory SchoolofBiochemicalEngineering,Indian NationalInstituteofAnimal InstituteofTechnology Biotechnology(NIAB)Hyderabad LaboratoryofBio-PhysioSensors Hyderabad,Telangana,India andNano-bioengineering Varanasi,UttarPradesh,India ISBN978-981-15-5822-1 ISBN978-981-15-5823-8 (eBook) https://doi.org/10.1007/978-981-15-5823-8 #SpringerNatureSingaporePteLtd.2021 Thisworkissubjecttocopyright.AllrightsarereservedbythePublisher,whetherthewholeorpartofthe materialisconcerned,specificallytherightsoftranslation,reprinting,reuseofillustrations,recitation, broadcasting,reproductiononmicrofilmsorinanyotherphysicalway,andtransmissionorinformation storageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodology nowknownorhereafterdeveloped. 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The registered company address is: 152 Beach Road, #21-01/04 Gateway East, Singapore 189721, Singapore Contents BiosensorsforClinicalSamples:ConsiderationandApproaches. . . . . . 1 MihaelaTertis,OanaHosu,AncaFlorea,andCeciliaCristea ImmunodiagnosisbyElectrochemicalMultiplexing inClinicalSamples. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 S.Campuzano,P.Yáñez-Sedeño,andJoséM.Pingarrón PrincipleandApplicationsofImmunodiagnosticsUsingRadioisotope asTracers. .. . . . . . . .. . . . . . . . .. . . . . . . . .. . . . . . . .. . . . . . . . .. . . 61 ShishuKantSuman,RohitSharma,andChandanKumar RedoxCyclingTechnologiesforPoint-of-CareImmunodiagnostics inVariousMatrices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 GorachandDutta,DespinaMoschou,andRiddhipratimMandal Next-GenerationImmunosensingTechnologiesBased onNano-Bio-EngineeredPaperMatrices. . . . . . . . . . . . . . . . . . . . . . . . 93 KuldeepMahato,BudhhadevPurohit,AshutoshKumar, AnanyaSrivastava,andPranjalChandra AlternativeAnalyte-BindingCompoundsforImmunosensor-Like Point-of-CareApplication. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 AnkitaKushwaha,YuzuruTakamura,andManishBiyani Bead-BasedSELEXforAptamersSelectionandTheirApplication inDetectionofDiverseAntigens. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125 DevikaN.Nagar,T.Yathirajarao,PankajKumar,PoojaKushwaha, andPankajSuman ChangingTrendsinImmunosensingTechnologiesandTheir CommercialAspectsinAnimalHealthandWelfare. . . . . . . . . . . . . . . . 141 SherinKaul,RajniSingh,SharanyaKamaraju,andPankajSuman MethodsforEnhancingAptamerAffinityforAntigenDetection andItsCharacterization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163 KomalBirader,L.SaiKeerthana,T.Yathirajarao,JeannieAliceBarla, andPankajSuman vv vi Contents ApplicationofBiosensorstoEnhanceReproductiveEfficiency andProductionofLivestockandPoultrybyDiverseAntigenAnalysis. . . 185 PankajKumar,ShaliniChakraborty,DevikaNagar,KomalBirader, andPankajSuman ClinicalImplicationsofCortisolandBioanalyticalMethods forTheirDeterminationinVariousBiologicalMatrices. . . . . . . . . . . . . 195 AjayKumarSahi,NeelimaVarshney,RakeshKumarSidu, SuruchiPoddar,Pallawi,KameshwarnathSingh, andSanjeevKumarMahto ReceptorsinImmunodiagnostics:Antibodies,AntibodyFragments, SingleDomainAntibodiesandAptamers. . . . . . . . . . . . . . . . . . . . . . . . 223 NachiketShembekar About the Authors PankajSuman isaScientistandPrincipalInvestigatorattheNationalInstituteof Animal Biotechnology, Hyderabad, India. He did his Bachelors in Veterinary Medicine from Maharashtra Animal & Fishery Sciences University, Nagpur, India and obtained his MS degree from the Indian Institute of Technology Roorkee, Roorkee, India. He did his Ph.D. at the National Institute of Immunology, New Delhi. He also worked as visiting scholar at Friedrich-Schiller University, Jena, Germany(2007–2010).Hisresearchexperiencespansthroughvaryingdisciplineof science pertaining to immunology, reproductive biology, veterinary, and clinical medicine. In the past, he has made a significant contribution in understanding the molecular mechanisms behind the immunological basis of pregnancy, vaccine delivery, and diagnostics. His current research is focused on the development of biosensing devices for the detection of small molecules (toxins, metabolites, etc.) throughdesigningsmallantibodiesandaptamersasatoolfordetection.Tillnow,he haspublishedover25researcharticlesinvariousreputedjournals.Heisarecipient ofseveralprestigiousfellowships andawardsfromagencieslikeCouncilofScien- tific&IndustrialResearch,India,DepartmentofScienceandTechnology,India,and United States Department of Agriculture, USA. He is also a recipient of the prestigious G. P. Talwar Young Scientist award for outstanding contribution in reproductivehealth.Hehasattendedseveralnationalandinternationalconferences anddeliveredinvitedtalks. PranjalChandra iscurrentlyemployedasanAssistantProfessorattheSchoolof Biochemical Engineering, Indian Institute of Technology (BHU), Varanasi, India. He earned his Ph.D. from Pusan National University, South Korea and did his postdoctoraltrainingatTechnion-IsraelInstituteofTechnology,Israel.Hisresearch focus is highly interdisciplinary, spanning a wide range in biotechnology, nanobiosensors, material engineering, nanomedicine, etc. He has designed several commercially viable biosensing prototypes that can be operated for onsite analysis forbiomedicaldiagnostics,environmentalmonitoring,andotherpoint-of-caretest- ing applications. He has published six books on various aspects of biosensors/ medical diagnostics from IET London, Springer Nature, and CRC Press, USA. He has also published over 85 journal articles in topmost journals in his research area including Biosensors and Bioelectronics, Analytical Chemistry, Biomaterials, vviiii viii AbouttheAuthors Chemical Communications, Electroanalysis, etc. His work has been greatly highlighted in over 300 topmost news agencies globally including Rajya Sabha TV; DD Science; Science Trends, USA; Nature India; Vigyan Prasar; Global Medical Discovery, Canada; APBN Singapore; Business Wire, Dublin; etc. He is a recipient of many prestigious awards and fellowships such as DST Ramanujan Fellowship (Government of India); Early Career Research Award (DST, Govern- ment of India); Brain Korea-21 and National Research Foundation Fellowship, South Korea; Technion Postdoctoral Fellowship, Israel; NMS Young Scientist Award, Biotech Research Society India Young Scientist Award, Young Engineers Award2018,RSCHighlyCitedCorrespondingAuthorAward(generalchemistry); ACS/ElsevierOutstandingReviewerAwards,etc.Heisareviewerofover50inter- national journals and expert project reviewer of various national/international funding agencies. He is Associate Editor of Sensors International and an editorial boardmemberofMaterialsScienceforEnergyTechnologiesbyKeAiandElsevier; WorldJournalofMethodology,USA;FrontiersinBiosciences,USA;andReportsin PhysicalSciences,Singapore. Biosensors for Clinical Samples: Consideration and Approaches Mihaela Tertis, Oana Hosu, Anca Florea, and Cecilia Cristea Abbreviations Ab Antibody AD Autoimmunediseases Ag Antigen AGA Antigliadinantibodies anti-CPP Anti-citrullinepeptide anti-tTG Anti-transglutaminaseantibodies AFP Alphafetoprotein AChE Acetylcholinesterase AP Alkalinephosphatase APTES 3-aminopropyltrietoxysilane Apt Aptamer ASV Anodicstrippingvoltammetry AuNPs Goldnanoparticles BSA Bovineserumalbumin c-Myc Myelocytomatosisoncogene cTnT Troponin-T cTnI CardiacTroponin-I CA125(MUC16) Cancerantigen125 CA19-9 Carbohydrateantigen19-9 CEA Carcinoembryonicantigen CA125 Carcinomaantigen125 CK-MB CreatinekinaseMB M.Tertis·O.Hosu·A.Florea·C.Cristea(*) AnalyticalChemistryDepartment,FacultyofPharmacy,“IuliuHaţieganu”UniversityofMedicine andPharmacy,Cluj-Napoca,Romania e-mail:[email protected] #SpringerNatureSingaporePteLtd.2021 1 P.Suman,P.Chandra(eds.),ImmunodiagnosticTechnologiesfromLaboratoryto Point-Of-CareTesting,https://doi.org/10.1007/978-981-15-5823-8_1 2 M.Tertisetal. CK-MM CreatinekinaseMM CNT Carbonnanotubes CSF Cerebrospinalfluid CRP C-reactiveprotein CV Cyclicvoltammetry CVD Cardiovasculardiseases ECL Electrochemiluminescence ELISA Enzyme-linkedimmunosorbentassay DDAB Didodecyldimethylammoniumbromide Den Dendrimer DPV Differentialpulsevoltammetry EGFR(ErbB-1;HER1) Epidermalgrowthfactorreceptor EIS Electrochemicalimpedancespectroscopy GADA Glutamatedecarboxylaseantibody GCE Glassycarbonelectrode GO Grapheneoxide hCG Humanchorionicgonadotropin HER-3 Humanepidermalgrowthfactorreceptor3 HE4 Humanepididymis-specificprotein4 HIgG HumanimmunoglobulinG HRP Horseradishperoxidase IgA ImmunoglobulinA IgB ImmunoglobulinB IgG ImmunoglobulinG IL-8(CXCL-8) Interleukin8 IL-17 Interleukin17 ITO Indiumtinoxide LF Lateral-flow LFTSs Lateral-flowteststrips LSW Linearsweepvoltammetry MBP Myelinbasicprotein MBs Magneticbeads MBP Myelinbasicproteins MDM2 Murinedoubleminute2 MH Mercaptohexanoicacid MIF Macrophagemigrationinhibitoryfactor MPR Myeloperoxidase MS Multiplesclerosis MUC4 Mucin4 Myo Myoglobin MWCNT Multi-walledcarbonnanotubes ND Neurologicaldisorders NSE Neuron-specificenolase NT Neurotransmitters PB Prussianblue