“HETEROSIS AND COMBINING ABILITY STUDIES FOR YIELD AND ITS COMPONENTS TRAITS IN SESAME (Sesamum indicum L.)” A THESIS SUBMITTED TO NAVSARI AGRICULTURAL UNIVERSITY NAVSARI IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE (AGRICULTURE) IN GENETICS AND PLANT BREEDING BY ARPANKUMAR JAGADISHBHAI NAYAK B. Sc. (Hons.) Agri. DEPARTMENT OF GENETICS AND PLANT BREEDING N.M. COLLEGE OF AGRICULTURE NAVSARI AGRICULTURAL UNIVERSITY NAVSARI – 396 450 GUJARAT JUNE – 2016 Registration No. 2010114053 DEDICATED TO MYBELOVED PARENTS AND MAJOR GUIDE Arpan Nayak .. ABSTRACT “HETEROSIS AND COMBINING ABILITY STUDIES FOR YIELD AND ITS COMPONENT TRAITS IN SESAME [Sesamum indicum L.]” Name of the student Major advisor NAYAK ARPANKUMAR J. Dr. S. R. Patel DEPARTMENT OF GENETICS AND PLANT BREEDING N. M. COLLEGE OF AGRICULTURE NAVSARI AGRICULTURAL UNIVERSITY, -396 450 ABSTRACT G g The present investigation on sesame comprised of a half-diallel set of eight parents and their 28 crosses. The experiment was laid out in randomized block design with three replications at college farm, N. M. College of agriculture, Navsari Agricultural University, Navsari during kharif, 2014-15. The observations were recorded as visual assessment [days to flowering and days to maturity, plant height (cm), number of branches per plant, number of capsules per plant, capsule length, seed yield per plant (g), 1000 seed weight (g), harvest index (%) and oil content (%) ] on randomly selected five competitive individual plants. The data were subjected to estimation of heterosis, combining ability analysis (Model-1, Method-2 of Griffing, 1956a). Analysis of variance revealed significant differences among the genotypes, hybrids and parents for all the traits except harvest index for parent studied. Several crosses exhibited significantly desirable heterobeltiosis and economic heterosis for seed yield per plant and other characters. On the basis of per se performance and estimates of heterobeltiosis, the crosses ASRT10 x AT 324 (41.88%) and ASRT10 x Patan 64 (41.88 %) were found to be most promising for seed yield per plant, hence could be evaluated further to exploit the heterosis or utilized in future breeding programme to obtain desirable segregants for the development of superior genotypes. Combining ability analysis revealed presence of both additive and non-additive gene effects. The estimates of general combining ability effects suggested that parents AT 164 and ASRT 10 were good general combiners for seed yield per plant and its related attributes. As regards specific combining ability effects cross combinations Vijapadi selection x ASRT 9 (3.29) followed by ASRT10 x Patan 64 (2.83) and ASRT10 x AT 324 (2.81) were significant for seed yield per plant. Looking to the role of additive as well as non-additive gene effects in the inheritance of most of the traits in the population under study, suggest diallel selective mating followed by heterosis breeding and biparental mating or recurrent selection breeding approaches for creation of more variability and to identify desirable transgressive segregants for further improvement of yield and yield component traits. On the basis of per se performance, heterosis and combining ability effects for seed yield per plant and its components, parents AT 164 and ASRT 10 and the hybrids ASRT10 x AT 324, ASRT10 x Patan 64 and Vijapadi selection x ASRT 9 were identified superior hybrids for their large scale testing. …………………………………………………………………………….......Abstract Dr. S. R. Patel Associate Professor, Dept. of Genetics and Plant Breeding, College of Agriculture, Bharuch campus, Bharuch. N. A. U., Navsari-396 450 C E R T I F I C A T E This is to certify that the thesis entitled, “Heterosis and Combining Ability Studies for Yield and its Components Traits in Sesame (Sesamum indicum L.)” submitted by Mr. Nayak Arpankumar Jagdishbhai in partial fulfillment of the requirements for the award of the degree of Master of Science (Agriculture) in Genetics and Plant Breeding of Navsari Agricultural University is a record of bonafide research work carried out by him under my guidance and supervision and that the thesis has not been previously formed the basis for the award of any degree, diploma or has been published for other similar title. All the assistance and help received during the course of the investigation have been duly acknowledged by him. Place: Navsari Date: June, 2016 Major Advisor (S. R.PATEL) D E C L A R A T I O N This is to declare that the whole of the research work reported here in the thesis for the partial fulfilment of the requirements for the degree of Master of Science (Agriculture) in Genetics and Plant Breeding by the undersigned is the result of investigation carried by him under direct guidance and supervision of Dr. S. R. Patel, Associate Professor, Dept. of Genetics and Plant Breeding, College of Agriculture, Bharuch campus, Bharuch. Navsari Agricultural University, Navsari- 396 450 and no part of the work has been submitted for any other degree so far. Place: Navsari Date: June, 2016 (Arpankumar J. Nayak) Countersigned by (S. R. Patel) Associate Professor Dept. of Genetics and Plant Breeding, College of Agriculture Bharuch Campus Bharuch N. A. U., Navsari-396 450 ACKNOWLEDGEMENT This memorable occasion of my life provides me a unique privilege to express my deepest sense of gratitude and indebtedness to my honorable Major Advisor, Dr. S. R. PATEL, Associate professor, Department of Genetics & Plant Breeding, College of Agriculture, N.A.U., Bharuch Campus, Bharuch. for his expert and intelligent guidance, constant encouragement, ever willing help, constant interest, constructive criticism and critical interaction throughout the course of this investigation and scrutinizing the manuscript right from the selection of my research problem up to the final shaping of this thesis in its present form. I am very grateful to my Minor Advisor, Dr. A. V. Narwade, Assistant Professor, Dept. of Genetics and Plant Breeding, N. M. College of Agriculture. I am equally thankful to my committee members Dr. R. K. Patel, Associate Professor, Dept. of Genetics and Plant Breeding, N. M. College of Agriculture, and Dr. H. N. Chhatrola, Assistant Professor Dept. of Statistics & Computer Center, ASPEE College of Horticulture and Foresty for their valuable comments and helpful suggestions during the course of this investigation. I wish to acknowledge Principal Dr. M. K. Arvadiya, N. M. College of Agriculture, Navsari for providing facilities during the course of my study. I heartly express my thanks to Dr. K. G. Modha, Dr. madhubala and Dr G. D. Vadodariya , and all the staff members of Department of Genetics and Plant Breeding, N. M. Agricultural, Navsari, for their valuable help and co-operation during the course of my study. The limited world of words hampers me to express the feeling of my indebtedness towards my friends whose love and affection always makes me feel at home here in the Navsari Agricultural University. I would like to thank my seniors Mandar, Dr. Haimil, Gaurav, Vipule, unnati, for their direct and indirect help to assist me reach this pinnacle. It is my pleasant duty to acknowledge most gratefully invaluable and unbelievable help received with joyful company from my friends gallery Aalok, Vishat, Ronak, Balat, Ravi, Ronak, Dubay, Haresh, Kiran, Chirag, Chelabhai. Dinesh, RJ, Jaykishan, Momin, Joshibhai, Joshi, Mali, Ravi, Sarjan, Bhagora, Vitthal, Pooja, Kalpana, and Reena during the course of this study. It is my pleasant duty to acknowledge most gratefully invaluable and unbelievable help received with joyful company from my juniors Mitesh, Suchi, Bhavna and Shubham and thanks to my all friends, who have inspired me in all respects during this study and I remain indebted forever to them. At this inexplicable moment of joy, words are inadequate in the available lexicon to express my gratitude and respect towards my family, who took pains to bring me up to this stage, without their love, sacrifice, extra-ordinary patience, inspiration and blessings this work would not have taken final shape. My heart takes breath when I realize the painstaking efforts taken up by My “Family Member’s” for making me post graduate of the noble profession. Words are just not sufficient to express my feeling for their love, blessing, affection and encouragement given to me by my parents Jagadishbhaai and Javanikaben and I solemnized to dedicate this thesis to them and vis-à-vis offers this as a humble tribute to science. My heartly lovable sisters Hema, Manisha, and Jalpa brother Hardik and my Fiancee Reetu. I special thanks to my beloved uncle S. M. Nayak and his son Monty and cousin brother Dilip Nayak who are deeply sited in my heart and always encourage every time. Last but far from the least, I bow my head in extreme regards to the almighty "JAY BHOLE” and “JAI RANGILA HANUMAN” whose blessings enabled me to reach this destination. Place: Navsari Date : / /2016 (ARPAN NAYAK) LIST OF SYMBOLS AND ABBREVIATIONS Symbols = is equal to X cross : Colon ( ) bracket ; Semi colon Abbreviations G gram et al. and others % per cent viz. namely C.D. Critical difference Cm Centimeter Contd… Continued C.V. Co-efficient of variation Max. Maximum Min. Minimum N. M. Naveenchandra Mafatlal No. Number R.B.D. Randomized Block Design S.Em. Standard error of mean F First filial generation 1 F. S. Full Sib H. S. Half Sib Gca General Combining ability Sca Specific Combining ability
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