Hairy Root Cultures Based Applications: Methods and Protocols PDF
Preview Hairy Root Cultures Based Applications: Methods and Protocols
Rhizosphere Biology Vikas Srivastava Shakti Mehrotra Sonal Mishra Editors Hairy Root Cultures Based Applications Methods and Protocols Rhizosphere Biology SeriesEditor AnilK.Sharma BiologicalSciences,CBSH,G.B.PantUniversityofAgriculture&Technology, Pantnagar,Uttarakhand,India The Series Rhizosphere Biology, emphasizes on the different aspects of Rhizo- sphere.Major increase inagricultural productivity, tomeet growing fooddemands ofhumanpopulationisimperative,tosurviveinthefuture.Alongwithmethodsof crop improvement, an understanding of the rhizosphere biology, and the ways to manipulateit,couldbeaninnovativestrategytodealwiththisdemandofincreasing productivity.ThisSerieswouldprovidecomprehensiveinformationforresearchers, and encompass all aspects in field of rhizosphere biology. It would comprise of topicsrangingfromtheclassicalstudiestothemostadvancedapplicationbeingdone in the field. Rhizoshpere is a dynamic environment, and a series of processes take place to create a congenial environment for plant to grow and survive. There are factorswhichmighthamperthegrowthofplants,resultinginproductivityloss,but, themechanismsarenotveryclear.Understandingtherhizosphereisneeded,inorder tocreateopportunitiesforresearcherstocomeupwithrobuststrategiestoexploitthe rhizosphereforsustainableagriculture. There are titles already available in the market in the broad area of rhizosphere biology, but there is a major lack of information as to the functions and future applications of this field. These titles have not given all the up-to-date information required by the today’s researchers and therefore, this Series aims to fill out thosegaps. Moreinformationaboutthisseriesathttp://www.springer.com/series/15861 (cid:129) (cid:129) Vikas Srivastava Shakti Mehrotra Sonal Mishra Editors Hairy Root Cultures Based Applications Methods and Protocols Editors VikasSrivastava ShaktiMehrotra DepartmentofBotany DepartmentofBiotechnology CentralUniversityofJammu InstituteofEngineeringandTechnology Samba,JammuandKashmir,India Lucknow,UttarPradesh,India SonalMishra SchoolofBiotechnology UniversityofJammu Jammu,JammuandKashmir,India ISSN2523-8442 ISSN2523-8450 (electronic) RhizosphereBiology ISBN978-981-15-4054-7 ISBN978-981-15-4055-4 (eBook) https://doi.org/10.1007/978-981-15-4055-4 ©SpringerNatureSingaporePteLtd.2020 Thisworkissubjecttocopyright.AllrightsarereservedbythePublisher,whetherthewholeorpart of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation,broadcasting,reproductiononmicrofilmsorinanyotherphysicalway,andtransmissionor informationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilar methodologynowknownorhereafterdeveloped. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt fromtherelevantprotectivelawsandregulationsandthereforefreeforgeneraluse. Thepublisher,theauthors,andtheeditorsaresafetoassumethattheadviceandinformationinthisbook arebelievedtobetrueandaccurateatthedateofpublication.Neitherthepublishernortheauthorsorthe editorsgiveawarranty,expressedorimplied,withrespecttothematerialcontainedhereinorforany errorsoromissionsthatmayhavebeenmade.Thepublisherremainsneutralwithregardtojurisdictional claimsinpublishedmapsandinstitutionalaffiliations. ThisSpringerimprintispublishedbytheregisteredcompanySpringerNatureSingaporePteLtd. The registered company address is: 152 Beach Road, #21-01/04 Gateway East, Singapore 189721, Singapore Preface The utilization of Agrobacterium has revolutionized the plant science research by offeringanexcellentresearchtool.Theproductionofhairyroots(HRs),asyndrome developedafterA.rhizogenes-plantinteraction,andtheestablishmentofhairyroot cultures(HRCs)systemhavelaidthebackgroundforbiotechnologicalproductionof plant-basedbioactivemolecules.Thesimilarorbetterproductionpotentialinhairy rootcultures(HRCs)hasattractedtheinterestofmanyplantscientiststoexplorethis techniqueintheareaofplant-basedsecondarymetaboliteproduction.Furthermore, new dimensions have also been explored to offer prospects to this technology to target issues related to environment remediation (phytoremediation), production of commerciallyvaluedmolecules(biotransformation),therapeuticproteins(molecular pharming),etc.Inthelastfourdecades,thejourneyhasbeenfascinating,andmany breakthroughshavebeenachieved.Torepresentthesebreakthroughs,recentlyinthe year2018,thebookHairyRoots:AnEffectiveToolofPlantBiotechnology(edited by V. Srivastava, S. Mehrotra, and S. Mishra) has been published. Nevertheless, diminutive documentation is available regarding experimental methods and pro- tocols related to this subject so far. Considering this as well as the significance of HRCsasbiotechnologicaltoolforvariouspurposes,inaconsecutiveendeavor,the presentbook,HairyRootCulturesBasedApplications:MethodsandProtocols,has been designed. The majority of book content consist of methodologies to develop experimentalprotocolsutilizinghairyrootbiotechnologyindetailhowever,someof thecontent(twochapters)existsindescriptiveform. Keeping in mind the requirement of updated basic information in hairy root biotechnology, Chap. 1 describes hairy root biotechnology podium (HRBP) as a researchplatform whereintegratedintra-andinterdisciplinary approachesareused toderivevaluablebenefitsofHRCsandalsodebatesonpromisesandprospectsin contrasttotherealitiesinhairyrootbiotechnology.Chapter2reviewsthepotential ofHRCsfortropanealkaloidproductionthroughvariousmethodsusedforHRCsof Solanaceae plant family. Chapters 3 and 4 deal with the protocols for hairy root induction of Coleus forskohlii and Swertia chirata and metabolite production of relevanttherapeuticimperativemolecules.Chapters5and6describetheprotocols v vi Preface for selection of spontaneous variants and culture condition optimization for increased alkaloid production in Catharanthus roseus, respectively. Chapter 7 explains elicitation as a potential strategy for enhanced withaferin-A production fromWithaniasomniferaHRCs.Chapter8describesproductionofbetulinicacid,a pentacyclictriterpenewithnoteworthypharmaceuticalactivities,throughtheestab- lishmentofHRCsofPentalinonandrieuxii.Chapter9outlinestheprotocolsrelated tothemaintenanceofHRCsofBrugmansia.candidaandalsodescribesthemethod for elicitation of HRCs with methyl jasmonate and other compounds followed by liberation of tropane alkaloids in the culture medium. Chapter 10 describes a detailed protocol for the establishment of hairy roots of Rubia. tinctorum, along with a protocol for elicitation and in situ removal of anthraquinones. Chapter 11 includesexperimentalapproachesinHRCsforbiomoleculeproductionandoptimi- zation ofstrategiesfor thesecretion ofrecombinant protein intheculturemedium. Chapter12providesacasestudyofteaplants(Camelliasinensisvar.sinensis)and claims for an improved protocol for Agrobacterium rhizogenes-mediated transfor- mationofrecalcitrantplantsforrootbiologystudies.Chapters13and14describe theutilityofAgrobacteriumrhizogenes-mediatedhairyrootsasabiologicalsystem forfunctionalanalysisofplantgenesrelatedtoarbuscularmycorrhizasymbiosisand legume nodulation, respectively. Chapter 15 describes a diagnostic panel for Fluidigm BioMark for the investigation of the expression of genes of alkaloid biosynthesis,transcriptionfactorsparticipatingintheregulationofalkaloidcontent, rolgenes,andreferencegenesassociatedwiththesynthesisofalkaloidsinNicotiana tabacumhairyroots. Precisely,thiseditedbookisanattempttopresentsomeoftheexcellentmethods andprotocolsrelevanttothisarea,whichcanbeservedasaneffectiveassettothose whowishtoworkonHRCs-basedresearch.Thisbookwillsurelyprovideenormous opportunitiesintheongoingandfutureresearchinthisfascinatingareaofresearch. Samba,JammuandKashmir,India VikasSrivastava Lucknow,UttarPradesh,India ShaktiMehrotra Jammu,JammuandKashmir,India SonalMishra Contents 1 HairyRootsBiotechnologyUnzipped:AJourneyofReality andPromises. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 ShaktiMehrotra,SonalMishra,andVikasSrivastava 2 HairyRootsasaSourceofTropaneAlkaloids. . . . . . . . . . . . . . . . 11 EwaSkałaandPrzemysławSitarek 3 Induction,MetaboliteAnalysis,andTransgenesisofHairyRoots fromColeusforskohlii. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 VijayLakshmiJamwal,IrshadAhmadRather,NitikaKapoor, andSumitG.Gandhi 4 EstablishmentofHairyRootsofEndangeredHimalayan PlantSwertiachirata:ASustainableAlternativetoExtraction fromNature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 RekhaChouhan,NatishKumar,AmitKumar,SajadAhmed, YadunandanSen,andSumitG.Gandhi 5 AProtocolfortheSelectionofSpontaneousVariants fromEstablishedCatharanthusroseusHairyRootCultures. . . . . . 65 FelipeA.Vázquez-Flota,MaríadeLourdesMiranda-Ham, MarinadelPilarNavarrete-Loeza,andMiriamMonforte-González 6 StrategiesfortheOptimizationofCultureConditions forIncreasingMetaboliteProductionThroughHairyRoot Cultures:MonoterpeneIndoleAlkaloidProduction inCatharanthusroseusHairyRoots. . . . . . . . . . . . . . . . . . . . . . . . 77 FelipeA.Vázquez-Flota,MaríadeLourdesaMiranda-Ham, JuliánCoello-Coello,andVíctorM.Loyola-Vargas vii viii Contents 7 ProtocolforEnhancedWithaferin-AProductioninElicited Withaniasomnifera(L.)DunalHairyRootCultures. . . . . . . . . . . . 87 AppakanShajahan,ChandrasekaranThilip, ValiyaparambathMusfirMehaboob,KunnampalliFaizal, PalusamyRaja,GanesanThiagu,andAbubakkerAslam 8 EstablishmentofHairyRootCulturesofPentalinonandrieuxii fortheProductionofBetulinicAcid. . . . . . . . . . . . . . . . . . . . . . . . 99 Yam-PucAlejandro,Sandoval-AntúnezCarlosAlberto, JoséEstebandelosSantos-Castillo,Avilés-BerzunzaElidé, García-SosaKarlina,Peña-RodríguezLuisManuel, Villarreal-OrtegaMaríaLuisa,andGodoy-HernándezGregorio 9 TropaneAlkaloidProductionbytheEstablishmentofHairy RootCulturesofBrugmansiacandidaandElicitation. . . . . . . . . . . 123 AlejandraB.Cardillo,MaríaPerassolo,JuanM.Minoia, JuliánRodríguezTalou,andAnaM.Giulietti 10 ElicitationasanEssentialStrategyforEnhancingAnthraquinone AccumulationinHairyRootCulturesofRubiatinctorum. . . . . . . . 133 MaríaPerassolo,AlejandraB.Cardillo,VíctorD.Busto, StéphanieRivière,JulietaCerezo,AnaM.Giulietti, andJuliánRodríguezTalou 11 EstablishmentofHairyRootCulturesfortheProduction ofBiopharmaceuticalsandOptimizationofMethodsfor RecombinantProteinSecretionintheCultureMedium. . . . . . . . . 153 EmileRage,SeleneBaschieri,CarlaMarusic,andMarcelloDonini 12 AnImprovedInVitroProtocolforAgrobacterium rhizogenes-MediatedTransformationofRecalcitrantPlants forRootBiologyStudies:ACaseStudyofTeaPlants(Camellia sinensisvar.sinensis). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175 LubobiFerdinandShamalaandShuWei 13 FunctionalAnalysisofPlantGenesRelatedtoArbuscular MycorrhizaSymbiosisUsingAgrobacteriumrhizogenes-Mediated RootTransformationandHairyRootProduction. . . . . . . . . . . . . . 191 TaniaHo-Plágaro,MaríaIsabelTamayo-Navarrete, andJoséManuelGarcía-Garrido 14 FunctionalCharacterizationofGenesInvolvedinLegume NodulationUsingHairyRootCultures. . . . . . . . . . . . . . . . . . . . . . 217 JawaharSingh,KamalKumar,andPraveenKumarVerma 15 FluidigmBioMarkDiagnosticPanelforAnalysisoftheExpression ofNicotianatabacumGenes,AssociatedwithAlkaloidSynthesis. . . 229 TatianaMatveevaandGitaAmini Editors and Contributors About the Editors Vikas Srivastava isworking as Assistant Professor in the Department of Botany, Central University of Jammu, India. He completed his Ph.D. jointly from Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP) and Lucknow University, India. Fur- thermore, he pursued Postdoc from the National Insti- tute of Plant Genome Research (NIPGR), New Delhi, India.Withspecializationinvariousaspectsofbiotech- nology,hehasacquiredtrainingandexperiencetocarry out research in diverse field of plant biology. He has published many articles in journals/books of interna- tionalreputeandhasbeenworkingasPrincipalInvesti- gator for major project sanctioned by University Grant Commission (UGC), New Delhi. He is a recipient of various awards and prestigious fellowships. He deliv- ered several invited lectures in institutes of national repute. Shakti Mehrotra is a Consulting Scientist in the Department of Biotechnology, Institute of Engineering and Technology, Dr. A.P.J. Abdul Kalam Technical University, Lucknow, India. She completed her Ph.D. jointly from CSIR-CIMAP and Lucknow University, India.Furthermore,shepursuedPostdocfromtheInsti- tute of Engineering and Technology, Lucknow (DBT-PDF)andCSIR-CIMAP(DSTYoungScientist). Withspecializationinvariousaspectsofbiotechnology, she has acquired training and experience to carry out ix
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