G Protein Signaling: Methods and Protocols (Methods in Molecular Biology Vol 237) PDF
233 Pages·2003·1.23 MB·English
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Preview G Protein Signaling: Methods and Protocols (Methods in Molecular Biology Vol 237)
Description:
A collection of cutting-edge methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. Among the readily reproducible techniques presented are those for the purification of G proteins and effectors enzymes, assays of these purified G proteins and effector enzymes, and for the study of G proteins interactions with effectors in intact cells. Additional methods are provided for assaying G protein coupled receptor structure, function, and localization; for studying the physiological roles for endogenous G proteins; and for examining lipid and phosphate modifications of RGS proteins.
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