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IN VITRO EMBRYO DEVELOPMENT IN THE PIG impact of oocyte maturation milieu on blastocyst morphology and viability Annadie KIDSON In vitro embryo development in the pig impact of oocyte maturation milieu on blastocyst morphology and viability Annadie Kidson 2004 Kidson, Annadie In vitro embryo development in the pig: impact of oocyte maturation milieu on blastocyst morphology and viability 2004 Dissertation Utrecht University, Faculty of Veterinary Medicine - with a summary in Dutch - ISBN 90-393-3730-6 Printing: DPP-Utrecht, Houten In vitro embryo development in the pig impact of oocyte maturation milieu on blastocyst morphology and viability In vitro embryo-ontwikkeling in het varken: effect van het milieu van eicelmaturatie op de morfologie en levensvatbaarheid van de blastocyst (met een samenvatting in het Nederlands) Proefschrift Ter verkrijgen van de graad van doctor aan de Universiteit Utrecht op gezag van de Rector Magnificus, Prof. Dr. W.H. Gispen, ingevolge het besluit van het College voor Promoties in het openbaar te verdedigen op donderdag 27 mei 2004 des ochtends te 10.30 uur door Annadie Kidson geboren op 10 April 1971 te Johannesburg, Zuid-Afrika Promotor: Prof. Ben Colenbrander Department of Farm Animal Health Faculty of Veterinary Medicine, Utrecht University Co-promotoren: Dr. Steph J. Dieleman Department of Farm Animal Health Faculty of Veterinary Medicine, Utrecht University Dr. Mart M. Bevers Department of Farm Animal Health Faculty of Veterinary Medicine, Utrecht University The investigations described in this thesis were financially supported by the Graduate School for Animal Health, the Department of Farm Animal Health of the Faculty of Veterinary Medicine of Utrecht University and Grant sponsor EU Commission Grant number QLK3-CT1999-00104 Printing of this thesis was financially supported by: Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University Dierenarts coöperatie AUV Graduate School for Animal Health, Faculty of Veterinary Medicine, Utrecht University Institute for Pig Genetics, Beuningen, The Netherlands Intervet Nederland BV TABLE OF CONTENTS Chapter 1 General introduction 1 Chapter 2 The influence of sow or gilt follicular fluid in vitro maturation 15 microenvironment, on fertilization, blastocyst morphology and apoptosis in prepubertal gilt oocytes Chapter 3 Effects of sperm dose and cumulus cells on the incidence and 37 pattern of polyspermy in prepubertal gilt oocytes, as modulated by sow or gilt follicular fluid during in vitro oocytes maturation Chapter 4 Effect of follicle-stimulating hormone on nuclear and cytoplasmic 53 maturation of sow oocytes in vitro Chapter 5 The effect of oviductal epithelial cell co-culture during in vitro 65 maturation on sow oocyte morphology, fertilization and embryo development Chapter 6 Quality of porcine blastocysts produced in vitro in the presence or 79 absence of growth hormone Chapter 7 Morphology-related cytoskeleton dynamics in porcine blastocysts 99 produced in vitro or in vivo, and its consequences on embryo viability Chapter 8 Summarizing discussion 119 Samenvatting 133 Acknowledgments 139 Curriculum Vitae 143 “Life is full of cactus, but we don’t have to sit on it.” Unknown Vir my familie, Vir Stephan, En vir Mart, Deo Gloria!
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