Flow Cytometry in Microbiology PDF
Preview Flow Cytometry in Microbiology
David Lloyd (Ed.) Flow Cytometry in Microbiology With 74 Figures Springer-Verlag London Ltd. David Lloyd, BSc, PhD, DSc Professor of Microbiology, University of Wales College of Cardiff, PO Box 915, Cardiff CFl 3TL, Wales Cover illustration: bis-(1 ,3-dibutybarbituric acid)-trimethine oxanol stained Eschericha coli NCTC 10418 (unpublished experiment of David Mason and David Lloyd) showing separation of viable and non-viable sub-populations. x axis: Forward Light Scatter. y axis: Fluorescence. ISBN 978-1-4471-2019-3 ISBN 978-1-4471-2017-9 (eBook) DOI 10.1007/978-1-4471-2017-9 British Library Cataloguing in Publication Data Flow Cytometry in Microbiology 1. Lloyd, David 576 ISBN 3-540-19796-6 Library of Congress Cataloging-in-Publication Data Flow cytometry in microbiology 1 David Lloyd, ed. p. cm. Includes bibliographical references and index. ISBN 0-387-19796-6: $120.00 (est.).-ISBN 3-540-19796-6 1. Flow cytometry. 2. Microbiology-Technique. 1. Lloyd, David, 1940- QR69.F56F56 1993 92-38958 576' .028-dc20 CIP Apar! from any fair dealing for the purposes of research or private study, or criticism or review, as permitted under the Copyright, Designs and Patents Act 1988, this publication may only be reproduced, stored or transmitted, in any form or by any means, with the prior permission in writing of the publishers, or in the case of reprographic reproduction in accordance with the terms of licences issued by the Copyright Licensing Agency. Enquiries concerning reproduction outside those terms should be sent to the publishers. © Springer-Verlag London 1993 Originally published by Springer-Verlag London Limited in 1993 Softcover reprint of the hardcover 1s t edition 1993 The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant laws and regulations and therefore free for general use. Produc! liability: The publisher can give no guarantee for information about drug dosage and application thereof contained in this book. In every individual case the respective user must check its accuracy by consulting other pharmaceutical litera ture. Typeset by Best-set Typesetter Ltd., Hong Kong. 12/3830-543210 Printed on acid-free paper Preface As yet, flow cytometry is not used so widely in microbiology as in some other disciplines. This volume presents contributions from research microbiologists who use flow cytometry to study a diverse set of problems. It illustrates the power of the technique, and may persuade others of its usefulness. Most of the con tributors gathered in Cardiff on 23 October 1991, at a meeting organized for the Royal Microscopical Society by Dr. Richard Allman, but the content of their chapters is not limited by the discourse of that meeting, and for balance other experts were invited to write for this book. Flow Cytometry in Microbiology thus represents the first collection of articles specifically devoted to the applications of a technique which promises so much to those investigating the microbial world. Cardiff, 1992 David Lloyd Contents List of Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix 1 Flow Cytometry: A Technique Waiting for Microbiologists David Lloyd . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2 The Physical and Biological Basis for Flow Cytometry of Escherichia coli Erik Boye and Harald B. Steen . . . . . . . . . . . . . . . . . . . . . . 11 3 Flow Cytometric Analysis of Heterogeneous Bacterial Populations Richard Allman, Richard Manchee and David Lloyd. . . . 27 4 On the Determination of the Size of Microbial Cells Using Flow Cytometry Hazel M. Davey, Chris L. Davey and Douglas B. Kell . . 49 5 Uses of Membrane Potential Sensitive Dyes with Bacteria David Mason, Richard Allman and David Lloyd . . . . . . . 67 6 Flow Cytometric Analysis, Using Rhodamine 123, of Micrococcus luteus at Low Growth Rate in Chemostat Culture Hazel M. Davey, Arseny S. Kaprelyants and Douglas B. Kell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 7 Characterization of Bacterial Cell Size and Ploidy using Flow Cytometry and Image Analysis Jerome Durodie, Kenneth Coleman and Michael J. Wilkinson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95 viii Contents 8 Cytometric Evaluation of Antifungal Agents Elizabeth A. Carter, Frank E. Paul and Pamela A. Hunter........................................... 111 9 Applications of Flow Cytometry in Bacterial Ecology Clive Edwards, Julian P. Diaper, Jonathan Porter and Roger Pickup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 10 Analysis of Microalgae and Cyanobacteria by Flow Cytometry Alex Cunningham . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131 11 Flow Cytometry at Sea Glen A. Tarran and Peter H. Burkill 143 12 Neural Network Analysis of Flow Cytometry Data Lynne Boddy and Colin W. Morris................... 159 13 Rapid Analysis of Microorganisms Using Flow Cytometry Michael Brailsford and Stephen Gatley................ 171 14 Appendix: Spectral Characteristics of Some Fluorescent Dyes and Excitation Sources David Lloyd . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181 Subject Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185 Contributors Richard Allman Microbiology Group, PABlO, University of Wales College of Cardiff, PO Box 915, Cardiff CF1 3TL, Wales Lynne Boddy Microbiology Group, PABlO, University of Wales College of Cardiff, PO Box 915, Cardiff CF1 3TL, Wales Erik Boye Department of Biophysics, Institute for Cancer Research, Montebello 0310, Oslo 3, Norway Michael Brailsford Chemunex, The Jeffreys Building, St. John's Innovation Park, Cowley Road, Cambridge CB4 1UF, England Peter H. Burkill Plymouth Marine Laboratory, Prospect Place, Plymouth PLl 3DH, England Elizabeth A. Carter SmithKline Beecham Pharmaceuticals, Great Burgh, Yew Tree Bottom Road, Epsom, Surrey KT18 5XQ, England Kenneth Coleman SmithKline Beecham Pharmaceuticals, Brockham Park Research Centre, Betchworth, Surrey RH3 7A J, England Alex Cunningham Department of Physics and Applied Physics, Strathclyde University, John Anderson Building, 107 Rottenrow, Glasgow G4 ONG, Scotland X Contributors Chris L. Davey Department of Biological Sciences, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales Hazel M. Davey Department of Biological Sciences, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales Julian P. Diaper Department of Genetics and Microbiology, Life Sciences Building, PO Box 147, Liverpool L69 3BX, England Jerome Durodie SmithKline Beecham Pharmaceuticals, Brockham Park Research Centre, Betchworth, Surrey RH3 7A J, England Clive Edwards Department of Genetics and Microbiology, Life Sciences Building, PO Box 147, Liverpool L69 3BX, England Stephen Gatley Chemunex SA, 41 Rue de 11 Novembre 94700, Maisons Alfort, France Pamela A. Hunter SmithKline Beecham Pharmaceuticals, Great Burgh, Yew Tree Bottom Road, Epsom, Surrey KT18 5XQ, England Douglas B. Kell Department of Biological Sciences, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales Arseny S. Kaprelyants Bakh Institute of Biochemistry, Russian Academy of Sciences, Leninskii Prospekt 33, 117071, Moscow, Russia David Lloyd Microbiology Group, PABlO, University of Wales College of Cardiff, PO Box 915, Cardiff CF1 3TL, Wales Richard Manchee Chemical Defence Establishment, Parton Down, Salisbury, Wiltshire SP4 OJQ, England David Mason Microbiology Group, PABlO, University of Wales College of Cardiff, PO Box 915, Cardiff CF1 3TL, Wales Contributors xi Colin W. Morris Department of Computer Studies, University of Glamorgan, Pontypridd CF37 1DL, Wales Frank E. Paul SmithKline Beecham Pharmaceuticals, Great Burgh, Yew Tree Bottom Road, Epsom, Surrey KT18 SXQ, England Roger Pickup Institute of Freshwater Ecology, Windermere Laboratory, Far Sawrey, Ambleside, Cumbria LA22 OLP, England Jonathan Porter Department of Genetics and Microbiology, Life Sciences Building, PO Box 147, Liverpool L69 3BX, England Harald B. Steen Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello 0310, Oslo, Norway Glen A. Tarran Plymouth Marine Laboratory, Prospect Place, Plymouth PLl 3DH, England Michael J. Wilkinson SmithKline Beecham Pharmaceuticals, Brockham Park Research Centre, Betchworth, Surrey RH3 7AJ, England
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