EVALUATION OF DIFFERENT POLLEN STORAGE METHODS IN SUNFLOWER (Helianthus

148 Pages·2016·6.78 MB·English

EVALUATION OF DIFFERENT POLLEN STORAGE
METHODS IN SUNFLOWER (Helianthus annuus L.)

By
CH.S LAKSHMI
B.Sc.(Ag)

THESIS SUBMITTED TO THE
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR
THE AWARD OF THE DEGREE OF

MASTER OF SCIENCE IN AGRICULTURE

DEPARTMENT OF SEED SCIENCE AND TECHNOLOGY
COLLEGE OF AGRICULTURE
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
RAJENDRANAGAR, HYDERABAD - 500 030

AUGUST, 2009
CERTIFICATE
Miss. CH.S. LAKSHMI has satisfactorily prosecuted the course of
research and that the thesis entitled “EVALUATION OF DIFFERENT
POLLEN STORAGE METHODS IN SUNFLOWER (Helianthus annuus
L.)” submitted is the result of original research work and is of sufficiently high
standard to warrant its presentation to the examination. I also certify that the
thesis or part there of has not been previously submitted by her for a degree of
any university.

Date : (K. KANAKA DURGA)
Place : Hyderabad Major Advisor
CERTIFICATE
This is to certify that the thesis entitled “EVALUATION OF DIFFERENT
POLLEN STORAGE METHODS IN SUNFLOWER (Helianthus annuus
L.)” submitted in partial fulfilment of the requirements for the degree of MASTER OF
SCIENCE IN AGRICULTURE (Seed Science and Technology) of the Acharya
N.G. Ranga Agricultural University, Hyderabad, is a record of the bonafide research
work carried out by Miss. CH.S. LAKSHMI under my guidance and supervision.
The subject of the thesis has been approved by the Student’s Advisory Committee.
No part of the thesis has been submitted for any other degree or diploma. The
published part has been fully acknowledged. All the assistance and help received during
the course of investigation have been duly acknowledged by the author of the thesis.

(Dr. K. KANAKA DURGA)
Chairman of the Advisory Committee

Thesis approved by the Student’s Advisory Committee

Chairman : (Dr. K.KANAKA DURGA) _____________________
Senior Scientist (Genetics & Plant breeding)
Seed Research & Technology Centre
Rajendranagar, Hyderabad – 500 030

Member : (Dr. A.R.G.RANGANATHA) _____________________
Principal Scientist (Plant breeding)
Directorate of Oilseeds Research
Rajendranagar, Hyderabad – 500 030

Member : (Dr. R. ANKAIAH) _____________________
Principal Scientist (Plant Physiology)
Seed Research & Technology Centre
Rajendranagar, Hyderabad – 500 030
DECLARATION
I, CH.S. LAKSHMI hereby declare that the thesis entitled “EVALUATION OF
DIFFERENT POLLEN STORAGE METHODS IN SUNFLOWER (Helianthus
annuus L.)” submitted to the Acharya N.G. Ranga Agricultural University for the
degree of MASTER OF SCIENCE IN AGRICULTURE is the result of original
research work done by me. I also declare that any material contained in the thesis has
not been published earlier in any manner.

Date : (CH.S. LAKSHMI)

Place: Hyderabad
CONTENTS
Chapter Title Page No.
I INTRODUCTION
II REVIEW OF LITERATURE
III MATERIALS AND METHODS
IV RESULTS
V DISCUSSION
VI SUMMARY
LITERATURE CITED
APPENDICES
LIST OF TABLES
Table
Title Page No.
No.
1a Analysis of variance for different pollen storage methods for
field observations in FRBD experiment
1b Analysis of variance for different pollen storage methods for
laboratory observations in FRBD experiment
2 Effect of filler material (F), storage methods (C) and age of
pollen (D) on seed set (%) in sunflower
3 Interaction effects of filler material (F) and storage methods
(C) on seed set (%) in sunflower
4 Interaction effects of filler material (F) and age of pollen (D)
on seed set (%) in sunflower
5 Interaction effects of storage methods (C) and age of pollen
(D) on seed set (%) in sunflower
6 Effect of filler material (F), storage methods (C) and age of
pollen (D) on seed yield plant-1 (g) in sunflower
7 Interaction effects of filler material (F) and storage methods
(C) on seed yield plant-1 (g) in sunflower
8 Interaction effects of filler material (F) and age of pollen (D)
on seed yield plant-1 (g) in sunflower
9 Interaction effects of storage methods (C) and age of pollen
(D) on seed yield plant-1 (g) in sunflower
10 Effect of filler material (F), storage methods (C) and age of
pollen (D) on filled seeds plant-1 in sunflower
11 Interaction effects of filler material (F) and storage methods
(C) on filled seeds plant-1 in sunflower
12 Interaction effects of filler material (F) and age of pollen (D)
on filled seeds plant-1 in sunflower
Table
Title Page No.
No.
13 Interaction effects of storage methods (C) and age of pollen
(D) on filled seeds plant-1 in sunflower
14 Effect of filler material (F), storage methods (C) and age of
pollen (D) on unfilled seeds plant-1 in sunflower
15 Interaction effects of filler material (F) and storage methods
(C) on unfilled seeds plant-1 in sunflower
16 Interaction effects of filler material (F) and age of pollen (D)
on unfilled seeds plant-1 in sunflower
17 Interaction effects of storage methods (C) and age of pollen
(D) on unfilled seeds plant-1 in sunflower
18 Effect of filler material (F), storage methods (C) and age of
pollen (D) on seed recovery (%) in sunflower
19 Interaction effects of filler material (F) and storage methods
(C) on seed recovery (%) in sunflower
20 Interaction effects of filler material (F) and age of pollen (D)
on seed recovery (%) in sunflower
21 Interaction effects of storage methods (C) and age of pollen
(D) on seed recovery (%) in sunflower
22 Effect of filler material (F), storage methods (C) and age of
pollen (D) on 100 seed weight (g) in sunflower
23 Interaction effects of filler material (F) and storage methods
(C) on 100 seed weight (g) in sunflower
24 Interaction effects of filler material (F) and age of pollen (D)
on 100 seed weight (g) in sunflower
25 Interaction effects of storage methods (C) and age of pollen
(D) on 100 seed weight (g) in sunflower
26 Effect of filler material (F), storage methods (C) and age of
pollen (D) on oil content (%) in sunflower
Table
Title Page No.
No.
27 Interaction effects of filler material (F) and storage methods
(C) on oil content (%) in sunflower
28 Interaction effects of filler material (F) and age of pollen (D)
on oil content (%) in sunflower
29 Interaction effects of storage methods (C) and age of pollen
(D) on oil content (%) in sunflower
30 Effect of filler material (F), storage methods (C) and age of
pollen (D) on field emergence (%) in sunflower
31 Interaction effects of filler material (F) and storage methods
(C) on field emergence (%) in sunflower
32 Interaction effects of filler material (F) and age of pollen (D)
on field emergence (%) in sunflower
33 Interaction effects of storage methods (C) and age of pollen
(D) on field emergence (%) in sunflower
34 Effect of filler material (F), storage methods (C) and age of
pollen (D) on pollen viability (%) in sunflower
35 Interaction effects of filler material (F) and storage methods
(C) on pollen viability (%) in sunflower
36 Interaction effects of filler material (F) and age of pollen (D)
on pollen viability (%) in sunflower
37 Interaction effects of storage methods (C) and age of pollen
(D) on pollen viability (%) in sunflower
38 Effect of filler material (F), storage methods (C) and age of
pollen (D) on endosperm / embryo ratio in sunflower
39 Interaction effects of filler material (F) and storage methods
(C) on endosperm / embryo ratio in sunflower
40 Interaction effects of filler material (F) and age of pollen (D)
on endosperm / embryo ratio in sunflower
Table
Title Page No.
No.
41 Interaction effects of storage methods (C) and age of pollen
(D) on endosperm / embryo ratio in sunflower
42a Dormancy duration studies in sunflower
42b Dormancy breaking methods in sunflower
43 Effect of filler material (F), storage methods (C) and age of
pollen (D) on germination (%) in sunflower
44 Interaction effects of filler material (F) and storage methods
(C) on germination (%) in sunflower
45 Interaction effects of filler material (F) and age of pollen (D)
on germination (%) in sunflower
46 Interaction effects of storage methods (C) and age of pollen
(D) on germination (%) in sunflower
47 Effect of filler material (F), storage methods (C) and age of
pollen (D) on seedling dry weight (mg) in sunflower
48 Interaction effects of filler material (F) and storage methods
(C) on seedling dry weight (mg) in sunflower
49 Interaction effects of filler material (F) and age of pollen (D)
on seedling dry weight (mg) in sunflower
50 Interaction effects of storage methods (C) and age of pollen
(D) on seedling dry weight (mg) in sunflower
51 Effect of filler material (F), storage methods (C) and age of
pollen (D) on seedling vigour index-I in sunflower
52 Interaction effects of filler material (F) and storage methods
(C) on seedling vigour index-I in sunflower
53 Interaction effects of filler material (F) and age of pollen (D)
on seedling vigour index-I in sunflower
54 Interaction effects of storage methods (C) and age of pollen
(D) on seedling vigour index-I in sunflower
Table
Title Page No.
No.
55 Effect of filler material (F), storage methods (C) and age of
pollen (D) on seedling vigour index-II in sunflower
56 Interaction effects of filler material (F) and storage methods
(C) on seedling vigour index-II in sunflower
57 Interaction effects of filler material (F) and age of pollen (D)
on seedling vigour index-II in sunflower
58 Interaction effects of storage methods (C) and age of pollen
(D) on seedling vigour index-II in sunflower
59 Effect of filler material (F), storage methods (C) and age of
pollen (D) on speed of emergence (%) in sunflower
60 Interaction effects of filler material (F) and storage methods
(C) on speed of emergence (%) in sunflower
61 Interaction effects of filler material (F) and age of pollen (D)
on speed of emergence (%) in sunflower
62 Interaction effects of storage methods (C) and age of pollen
(D) on speed of emergence (%) in sunflower
63 Seed set (%) at different starvation periods (days) of stigma
emergence in sunflower
64 Correlation coefficients among seed yield and yield attributing
characters with age of pollen in sunflower