Epithelial Transport: A guide to methods and experimental analysis PDF
Preview Epithelial Transport: A guide to methods and experimental analysis
Epithelial Transport JOIN US ON THE INTERNET VIA WWW, GOPHER, RP OR EMAil: WWW: http://www.thomson.com GOPHER: gopher.thomson.com A service of ICDP FTP: ftp.thomson.com EMAIL: Epithelial Transport A guide to methods and experimental analysis Edited by Nancy K. Wills, Luis Reuss and Simon A. Lewis Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas, USA CHAPMAN &. HAll London· Weinheim . New York· Tokyo· Melbourne· Madras Published by Chapman & Hall, 2-6 Boundary Row, London SEl 8HN, UK Chapman & Hall, 2-6 Boundary Row, London SEI8HN, UK Chapman & Hall GmbH, Pappelallee 3, 69469 Wemhelm, Germany Chapman & Hall USA, 115 Fifth Avenue, New York, NY 10003, USA Chapman & Hall japan, lTP-japan, Kyowa BUlldmg, 3F, 2-2-1 Hirakawacho, Chlyoda-ku, Tokyo 102, japan Chapman & Hall Austraha, 102 Dodds Street, South Melbourne, Victona 3205, Austraha Chapman & Hall India, R. Seshadri, 32 Second Mam Road, CIT East, Madras 600 035, India Fust edition 1996 © 1996 Chapman & Hall Softcover reprint of the hardcover 1st edition 1996 Typeset m 1O/12pt Palatmo by AFS Image Setters Ltd, Glasgow ISBN-13: 978-94-010-7168-0 e-ISBN-13: 978-94-009-1495-7 001:10.1007/978-94-009-1495-7 Apart from any fair dealing for the purposes of research or private study, or Criticism or reView, as permitted under the UK Copyright DeSigns and Patents Act, 1988, this pubhcatlon may not be reproduced, stored, or transmitted, in any form or by any means, without the pnor permissIOn in writmg of the pubhshers, or m the case of reprographic reproduction only m accordance With the terms of the licences issued by the Copyright Licensing Agency in the UK, or in accordance With the terms of licences issued by the appropriate Reproduction Rights OrgamzatlOn outside the UK. EnqUiries concermng reproduction outside the terms stated here should be sent to the publishers at the London address printed on this page. The publisher makes no representation, express or imphed, With regard to the accuracy of the mformatlon contamed m thiS book and cannot accept any legal responslbihty or liability for any errors or omiSSIOns that may be made. A catalogue record for this book IS available from the British Library I§ Printed on acid-free paper, manufactured in accordance WIth ANSI/NlSO 239.48-1992 and ANSI/NlSO 239.48-1984 (Permanence of Paper) Contents List of contributors Vll Preface xi 1 Epithelial structure and function 1 Simon A. Lewis 2 Epithelial transport proteins 21 Luis Reuss, Nancy K. Wills and Simon A. Lewis 3 Epithelial polarity 49 M. Cereijido, R.G. Contreras, M.R. Garcia-Villegas, L. Gonzales-Mariscal and J. Valdes 4 Characterization of epithelial ion transport 70 Calvin U. Cotton and Luis Reuss 5 Epithelial electrophysiology 93 Simon A. Lewis 6 Impedance analysis of epithelia 118 Simon A. Lewis, Chris Clausen and Nancy K. Wills 7 Measurements and interpretation of cytoplasmic ion activities 146 Guillermo A. Altenberg and Luis Reuss 8 Measurements of epithelial cell volume 167 Luis Reuss, Calvin U. Cotton and Guillermo A. Altenberg VI Contents 9 Methods and experimental analysis of isolated epithelial cell membranes 190 Austin K. Mircheff 10 Methods and experimental analysis of single ion channels 212 Simon A. Lewis 11 Epithelial cell culture 236 Nancy K. Wills 12 Signaling pathways regulating ion transport in polarized cells 256 Norman J. Karin, Min LN. Zhang, E. Radford Decker and Roger O'Neil 13 The cytoskeleton and epithelial function 275 John W. Mills 14 Future perspectives: molecular biology and pathophysiology 306 Alicia McDonough Appendix A: Instrumentation 323 Appendix B: Solving simple equivalent circuits 325 Appendix C: A spread-sheet for marker distribution data 329 Index 335 Contributors Guillermo A. Altenberg MD Department of Physiology and Biophysics University of Texas Medical Branch Galveston Texas 77555-0641 USA M. Cereijido MD, PhD Centro de Investigation y de Studios Avanzados Fuselage, Biofisica y Neurociencias Apartheid Postal 14-740 07000 Mexico DF Mexico Chris Clausen PhD Department of Physiology and Biophysics State University of New York at Stony Brook Stony Brook New York 11704-8861 USA R. G. Contreras PhD Centro de Investigacion y de Estudios A vanzados Fisologia, Biofisica y Neurociencias Apartado Postal 14-740 07000 Mexico DF Mexico Calvin U. Cotton PhD Department of Physiology Case Western Reserve Cleveland Ohio 44106-4948 USA viii Contributors E. Radford Decker PhD Department of Pharmacology Texas Biotechnology Corporation Houston 77030 Texas USA M. R. Garda-Villegas MD Centro de Investigacion y de Estudios A vanzados Fisologia, Biofisica y Neurociencias Apartado Postal 14-740 07000 Mexico DF Mexico L. Gonzalez-Mariscal MD Centro de Investigacion y de Estudios Avanzados Fisologia, Biofisica y Neurociencias Apartado Postal 14-740 07000 Mexico DF Mexico Norman J. Karin PhD Department of Integrative Biology University of Texas, Houston Health Science Center Houston Texas 77030 USA Simon A. Lewis PhD Department of Physiology and Biophysics University of Texas Medical Branch Galveston Texas 77555-0641 USA Alicia McDonough PhD Department of Physiology and Biophysics University of Southern California, School of Medicine 1333 San Pablo Los Angeles CA 90033-1026 USA Contributors IX John W. Mills PhD Department of Biology Clarkson University Potsdam NY 13676 USA Austin K. Mircheff PhD Department of Physiology and Biophysics University of Southern California, School of Medicine 1333 San Pablo Los Angeles CA 90033-1026 USA Roger O'Neil PhD Department of Integrative Biology University of Texas, Houston Health Science Center Houston Texas 77030 USA Luis Reuss MD Department of Physiology and Biophysics University of Texas Medical Branch Galveston Texas 77555-0641 USA J. Valdes PhD Centro de Investigacion y de Estudios A vanzados Fisologia, Biofisica y Neurociencias Apartado Postal 14-740 07000 Mexico DF Mexico Nancy K. Wills PhD Department of Physiology and Biophysics University of Texas Medical Branch Galveston Texas 77555-0641 USA x Contributors Min LN. Zhang MD Department of Integrative Biology University of Texas, Houston Health Science Center Houston Texas 77030 USA
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