Engineering Biosensors: Kinetics and Design Applications

419 Pages·2001·15.18 MB·English

by  Ajit Sadana

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Engineering Biosensors:
Kinetics and Design
Applications
Engineering Biosensors:
Kinetics and Design
Applications
Ajit Sadana
Chemical Engineering Department
University of Mississippi
University, Mississippi
ACADEMIC PRESS
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Copyright 0 2002 by Academic Press
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This book is dedicated to my parents,
Dr. J. C. Sadana and Mrs. Jinder Sadana, to whom I owe more than
they will ever know.
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CONTENTS
Preface xi
1 Introduction
1.1. Background, Definition, and the Need for Biosensors 1
1.2. Assay Formats 10
1.3. Difficulties with Biosensor Applications 12
1.4. Newer Applications for Biosensors 13
1.5. Commercially Available Biosensors 17
1.6. Biomedical Applications 17
1.7. Overview 19
2 Influence of Diffusional Limitations and Reaction Order
On Antigen-Antibody Binding Kinetics
2.1. Introduction 23
2.2. Theory 24
3 Influence of Diffusional Limitations and Lateral Interactions
on Antigen-Antibody Binding Kinetics
3.1. Introduction 45
3.2. Theory 46
3.3. Conclusions 63
vii
viii Contents
Fractal Reaction Kinetics
4
4.1. Introduction 67
4.2. Fractal Kinetics 69
5 Influence of Different Parameters on Fractal Dimension
Values During the Binding Phase
5.1. Introduction 83
5.2. Theory 85
5.3. Results 89
5.4. Summary and Conclusions 122
6 Fractal Dimension and the Binding Rate Coefficient
6.1. Introduction 127
6.2. Theory 130
6.3. Results 133
6.4. Conclusions 183
7 Fractal Dimension and the Dissociation Rate Coefficient
7.1. Introduction 187
7.2. Theory 190
7.3. Results 195
7.4. Conclusions 216
8 Influence of Nonspecific Binding on the Rate and
Amount of Specific Binding: a classical analysis
8.1. Introduction 221
8.2. Theory 230
9 Influence of Nonspecific Binding on the Rate and Amount
of Specific Binding: a fractal analysis
9.1. Introduction 253
9.2. Theory 255
9.3. Results 257
9.4. Other Examples of Interest 265
9.5. Conclusions 269
ix
Contents
10 Fractal Dimension and Hybridization
10.1. Introduction 273
10.2. Theory 275
10.3. Results 276
10.4. Conclusions 307
11 Fractal Dimension and Analyte-Receptor Binding in Cells
11.1. Introduction 311
11.2. Theory 313
11.3. Results 315
11.4. Conclusions 34 1
12 Surface Plasmon Resonance Biosensors
12.1. Introduction 345
12.2. Theory 347
12.3. Results 349
12.4. Conclusions 379
13 Economics and Market for Biosensors
13.1. Introduction 385
13.2. Market Size and Economics 386
13.3. Development Cost of a Biosensor 393
13.4. Cost Reduction Methods 395
Index 399