DTIC ADA436074: High Sensitivity Detection of Bacterial Endospores via TB Photoluminescence Enhancement PDF
Preview DTIC ADA436074: High Sensitivity Detection of Bacterial Endospores via TB Photoluminescence Enhancement
TYETDIVITIS NHEGSIH LAIRE T NCFOAOIBTCE SE RAOIPVS OBDTNE ECNNEECSENIMULOTOHP TNEMECNAH eipselli G. Bsema Jdn a,onirgelle P. Mlua P,*.r J,lle F. FsalohciN U.S. A ymr R hcraese L aboratory , O scitp B hcnar 2800 P owder M lli R d ,.A ihpled , DM38702 CARTSBA T ,yrtsime hlcacitylanao i obetgnella hlcaciti r sacsieropsod nleairetc agbnitceteD ero pes h ftsorebm e ymdbesu aec rsamelbo rhptla edh nsaesaes isduoir e fsroebm ueancnis - aren eggnimrof Bacillus d na muidirtsolC evitisne sylhgi hadepoleve deva he .W droo hftem sdioht e.r mlumO/U F0C0 0n5a hsts efslotim inloitcet eddetartsnom eedv ad hnnaoitcet erdieht . )ap dd(i ccainilocip i,dseropsod nleairetc andbinuopm orcek r faeaomcnese re p hndtoesab ae ped h,t VeU h ntdietic xde n bahTt idwexelpm oncehW ecnecsenimuloto hep hstecnahn . eduting a fmsored rloarev e ys bb fTnooissime d nsalairet almacigolo irbeh tmoo refcnerefret nri olfaitnet oep hdtetagitsev neiv aehW evitis o sapeuv ilgl isweropsod nleairetc anba hrteh tgoniht otna hdtnu odf nsalacimehc esn opser oe tt s a.in ht hodpsot i esestetewotla catoh ahngahpe teihsoh frstpntrooessaureogOpvhrnpoi e hhtguor hmtelbo rspi hetmocre v ooetl bnae eebv a. ehsWlang idsevres beo hetcud elrl iswnoi lCl Af onoitidda deuctnseref r s e.tfrtloun uoiesheTr elbl isweidu testahpso hdp nsaei 3 .detneserp ndiedneps ue srsaeropsod ne ehnte hdwesael esar ipe d hfstos er%l o0y1l neocniS stlus er.r ua Op fdeosael eer hgtnicnah nre osfdoht edmenima xoes leav a eh,wreff usbuoeuqa lacimeh cdn alacinahce mhto bmorf t s .ee dbheTtnese relpbl iewsael ea rpedcnah nosetdohtem 0 2as ideveihc aeva hew - mor fesaele rap dn iesaercn idlof B. globigii setun in2smieropsod ne .C° 0og8tnita ed hneanimalyced ofndooitid de ahhtguorht INTRODUCTION ire t ncfoaoibtce teehdT lacitylanaoi bn iegnellah ctnacifingi sas iseropsodn ela aren eeg h ftsorebm eemm o fseolc yecf iel h nteiga ttsnamr o sadeiropsod n ee. hyTrtsimehc Bacillus d na muidirtsolC do or,fogninosi odpo o,fesaes iedsu an casceice pesse hfrt oe.b m uAn ,egaliops edwoht e em. hsTrotc eysratil idm nnaailiv iec hhtt o nbtinatrop m sinioitcet erdie h ots tsr irf odfednet n siriep aspi h nteibircsed - sdioht eem httnemyolp eddednet nsi i .h net Isturela 1 Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. 1. REPORT DATE 2. REPORT TYPE 3. DATES COVERED 00 JAN 2002 N/A - 4. TITLE AND SUBTITLE 5a. CONTRACT NUMBER High Sensitivity Detection Of Bacterial Endospores Via Tb 5b. GRANT NUMBER Photoluminescence Enhancement 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION U.S. Army Research Laboratory, Optics Branch 2800 Powder Mill Rd., REPORT NUMBER Adelphi, MD 20783 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM(S) 11. SPONSOR/MONITOR’S REPORT NUMBER(S) 12. DISTRIBUTION/AVAILABILITY STATEMENT Approved for public release, distribution unlimited 13. SUPPLEMENTARY NOTES This article is from ADA409494 Proceedings of the 2001 ECBC Scientific Conference on Chemical and Biological Defense Research, 6-8 March , Marriott’s Hunt Valley Inn, Hunt Valley, MD. 14. ABSTRACT 15. SUBJECT TERMS 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF 18. NUMBER 19a. NAME OF ABSTRACT OF PAGES RESPONSIBLE PERSON a. REPORT b. ABSTRACT c. THIS PAGE UU 7 unclassified unclassified unclassified Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std Z39-18 sesaerc nliev ellang ies hnte hdw ndanuorgkc aeb hrtotin o omdtesu s itrel an a,yltnacifingi s em id tncaifice pesr ormeh troeggi rodtteussi -.seuqinhc egtnimusnoc eropsod ne e hn)ti)apd(a Ce(tanilocip imduicl afecocnese re p hndtoes asdbioht ermuO scerops o.d nnoEitul oost na ipsdi hfetom ofesosael ee rhd tnganisac thg in %yei2o5bwat1tno 1 fo,apd 1 bfnTooit i.d sdrAotc arfeh td onsaeice pngsonidnep ed 3+ l lniowitu lsousoe u eqohatt a ped hhtt inwoitaxelpm o nctiluser 2 - ttahg iVhlUt idwetaidar r ni.e ehrWo pe shmto rdfesael er )apd(b T,[mumix anmoitpros ba apedht ] 3 -2n derapmo cecnecsenimu ldecnahn eyltaer gastibihx e n to Tb 3+ sdi nhatgnelev anwoitatic xee hmto rrf adfetfi h sseicnecsenim udlevres b eo. heTno la beT h ftnooitce t. esDerutang iescnecsenim uellucel olmamr onna hrteworran 3+ ecnecsenimu l seirutangis .s enrIopso dlnaeiret csa u bd haenftpcaodnes e e rrhrpotefk reau mqe iranoufereht bfsTosec xnee a sesuwtnemirep xreuo 3+ ])apd( bxsTei[lp m eomfhc rot eo,ohfst + . suoiverP ap dd(nag ie lhmto rrfefsna rytgre ntea hdtenimret es dahhcraeser 2 -) to the t seta tdsetic xmeuibre .tnemecnah nsei h otstdael 2,3 e h ftnooisneps ussuoeu q n afatonuo mlala m,asdoht esm i h ntI lC bdTereff u oabdted d saeitylana odteretl ief rsaetalucitr agpni niatn oscelpm a ys. nnAoitulos 3 e rteeahtltaowsi - . shdTnuopm oeclbulos htgnelev ahawt idwetaidar rnie h steilpm aes smiurtce pnsoissi meecnecsenim uel hdt nmaumix anmoitpros baa ped h otgtnidnopserroc bnTa hyttisnet nnioissi mreegnor tsstibih xtea hetlpm a ys. ndAetcelloc 3+ sniatn oecno la .seropso dlnaeiretcab ruOcraeser fnooitanima xee hst atws r. isFeidu trsoj aemer hott ndiediv inde esb ahh .doht enmoitcet erd uhot iewcnerefret nri osflairet almacigolo idb nlaacime hscuoirav 4 si h T gnits eetvitis oeps l. asFtlus eervitag edn neavitis oepsl ahft ogbnitagitsev ndievlovni rdoefkc ehc gnit seevtita g ee.sns learFopso dlnaeiret cnaibat n totooc adnshetlp m rasosefsnopser lairetc agbniniatn oscelpm amso rlfang i fsnooitanimi l renooitcud eer hetre hswes adcetagitsevni gnid ndiefnrec nyodc udt ns.o dc eeehrsTru csceoropsodne suoirete leeehcd tu edovetirti dndaa .euqinhc er tuhot itwnerefret nyirami re ph,tetahpso hftpoceffe 6 detagitsevn ieva he w,yllan i F ftoim ie lhetvorp morited rnsoieropsod nleairetc ae bhmto ra fpedr oemsael eosrtdohtem . enfs ooas iohintphIcteted ostdoht elmacime hdc nlaacinahc elmarev edsenima x eekwr orwu extract more dpa. LATNEMIREPXE r u noliiat e nddiebircs eed rtanemegnar rlaacit pdo nsaerudeco rlpatnemirep xeehT .snoitacilb uspuoiverp 4 -6 reb rsatnemirep x fepouo rhgc a nesielpm a es hT ought to a constant dn ab Tf oleve leh tdn areffu bhti wemulov B. globigii timr eot ptehsc aneiht itwnatsn odcl ee hra e e.r 6h .T Ht7rpaeff uabmzi rsTuoeu qneaid aemr eswelpm ae s hf.lt oslnAosirapm orceporp pm ahsc a neeod aem rsatnemerusa eemtacilper ssietacilp eerer het hrt oeful aevgare vea hdt neal ssielpm a fsto ehsc ameo rlfang i es. hsTto llp l nadi nsanoitalucl agcnideecc urs odfesu .emu lloav t donntoaitartnec neorcopso dlnaeiret cea mb eahhs ttdirwad ndo aetatzsilamron STLUSER DNAISSUCSID NO INTERFERENCE STUDY. enimrete do tsessal ceerh tn islaireta mdenimax eeva he W .euqinh cneotitce teerdopso dlnaeiret ctrnacuobeo frfieeht 4,5 cinag reon idnekce h ecW esl ahft orb osflairet almacigolo inbeetru odf n,astl acsinagro nniev e,sslacimehc d neavitisop lairetc anaba hrteh tloairet aname hswruc ceovitis oeps l. asAfesnops eervitag eenslaf eav adgenima x eeswlairet aem h fte on. odNnuorgkc a bbeT hetvo blaang ieasv iegropsodne 2 dlei ytah tslaireta myln oeh t,tca fn .Ilangi sevitiso peslaf lairetc aebr eewsnops eerviti s doaep fsoelpm ao swr tosfesnops ee rhstedul c en1ri u.eg 1riuFg innFie es,saseropsodne B. globigii .seropsod nleairetc ambo rsfesnops eerviti s . oe1PrugiF poT - deredwop B. globigii 6.165 × 10 8 ,Lm/ UFC esff oytisnetni eldd i;M00 0y+t4b -ediciruhT ® 8% .g0niniatn o,c B. thuringensis , 0fr4oot c ydaabeftul i,d mott o;B00 0 y+2tbesf fyotisnetni - siretS B. globigii 1.3 × 10 9 .Lm/ UFC %8. 0gniniatno cedicitcesn ilaicremmo cadna .Bsisnegniruht . s ierheTtS B. globigii saw ngininiam ear ped hmto rlfang ies httcet e odetl blali tesr e ewdw nnaoitagufirtn e ycdbehsaw the spores. devres b eoswlang ies h ntsinoitcud e nrtilus edr itd etss eet h ntsilairet aem h ftloareveS ftonuo mdaex imaforf B. globigii itluciffi dem o .S e hfrtoeb m huatn idwevres boos lear eswe ez ihsguor hdtevom e ernb asclairet aemse htt u,bsdl odm nsanellop - ecni sgnilpma sevitceles nfooiti dhd tadiewrru cec so ta.sc sr eeohrwTopso dlnaeiret cna abrhetg rha c leuyrmeaht etahpsohp -.slacimeh cgniniatnoc K HPO al e.r salBang ir suntoocef fseuoiretel etds oe mhd tah 2 4 e h nttocef fgenitibih nrialim isas arheff uebtahpso htpa hdtetrop eerv ayhrre hdSna .etanilocipi dmuibre tmor fnoissim etnecsenimulotohp 7 si hrt oeflbiss oep rsanoitanalp xoe wT noitavresbo brT oyftinif fhag isah anhoi neatahpso hep htta h sttisr i ef. hT +3 ecalpsi dya mdn a ye hetrof esbelucel oam pddetic xee hhtcne uyq anmoi neatahpso hep h,tyldno c. e tSmio rafpd yeobghtTrt erni eerhetfsnart +3 d deerehtoetafnrtsn os.mtel due sseerhT h t l idaowoethd taem .ecnerefre tentiahpsohp PHOSPHATE INTERFEREN CE. stnemirep xft eoet sx er nuO 6 agnidni fn odesuco f suoive re phndtiebircs esdetahpso hftpocef fseuoiretel ee dhettanimi lreeocud eodrtohtem .t nad ohn ituetoWcfes B. globigii pma s elbitpecs usss eelr edwnuo rgGnivo ryPawg umDo rsfel lanoiti d ed eohaesuttcidnatsi sse irth atehltb i.se sntso oIip alnpa adshettahps oohtp 3 stnenopmoc 8 lanoitid de ahetti p.s genDisseco rd pnhatwo rrgie hfttolu s sseaaerlpm aesso hnti ”evictcetorp“ yawg u fDsoelpm a,sstnenopmo B. globigii f olangi sn iesaerce dadecneirepx ellit s up to 15% when exposed to 85 m KM HPO nfooKitartne c .neo m ceathsAt HPO en o al,pad 2 4 2 4 . htgner tlsang inesisaerc e% opdd5atue6reffus osttl acsinagro nsiuoir afev ose uhetnima xose tanwoitagitsev nr i unpoie ttsx eenhT s ew .tro2suh egstiaFhp s eofchonperefre teenhcituder . enWoitanima xsei h ftsotlus eerh 1 8.0 6.0 4.0 Normalized Intensity 2.0 0 sohp on reffuB CaCl2 FeCl3 CeCl3 3)3ON(Y 3)4OS(2lA 3lClA e.r2 ugiF on (ytisnetn ieh to tdezilamro ns ilangi se h .Tsnoitulo stla sf onoitidd an oyrevoce rlangiS l a fseomul oev hrt oeftasnepm o ocdted draeff uhbt ikwco taspd/ beT h ft)oetahpsohp . ded dnaoitul ots odted draeff uhbt ikwco testahpsohp/apd/ beT h ftloev ellang idsezilamr oen hsttneserp erreffuB l Af ostceff elaicifene be h .Tdedd anoitulo stla sf oemulo veh tro fetasnepmoc OS( ) lCl Adn a . rearealc 2 4 3 3 ta chti wstla sn odetartnecnoc l Atah tdetrope rnee bsa ht I.egrah c3 + ahti wsnoi +3 reta wn ides us i .sno ietahpsoh pevome ro tseitilica fgnissecorp mor ftnediv eylrael cs9 ihcaorpp asih tf oytilit ueh T to nere w Odegrah cylevitage n agniniatno csnoin ahti wstla seh T.stluse reseht devre sdn aevitceffe hgi heh tf otluse rtceri d as isih T.noita ceh tf ostceff elaicifene beh tredni hr olangi seh tecude rot b Tf oytiniffa +3 .snegyx odegrah cylevitage nro f 2, 3 lCl Aneh W ap dgniniatno cselpma so tdedd asa w 3 yawgu Dro iigibol g. B seh t, eh tro fdeniatnia mdn aap dro f%5 1o tp uy bdecude rsa wleve llangi yawguD iigibol g.B 5 8o terusopx en o m M K HPO eh tt ar oerofe bdedd asa wap deh ttah tdedivor p, 2 4 l Af onoitidd af otceff elaicifene beh tswoh syllacihpar gsih T.etahpsoh peh ts aemi temas lC . 3 MECN AEHSNAE EALPEDR ENT. sntoro ftfnee ct esrd rouemosu ce ovefaWh ecn i,sseropsod nleairetc aeb hmto raf p fdeosael eer hgtnicnah n fedooht etms eeb hgtninimreted . sIreff usbuoeu q nanioisneps u ysdbesael e srai pedlbalia vea h ft%o 0n1a hstsel eywdu tssi hnt fnooisneps ulsaicremm odcehs adawesu B. globigii f oleve lrewo lyltnacifingi sada htah t yawg ueD hnta hrteff usbuoeu q nanioisneps unso pau pedlbalia vyalidaer B. globigii . edW id rtx ef osnae mlacimeh cdn alacinahce mhto bdenimax eevah e hmto ra fpedr ogmnitca . see lrboapTsodne 1 .stnemirep xeese h ftsotlus eer hsteziramm us 4 ELBAT mumixa meh tf ooita reh ts itn1emecnahn emumixa me h .Tsdohte mesaele rAP Df oyramm u .S necno cema seh thti wdradnat serutarepme tmoo reh tdn adeveihc alangis .seropsod nleairetc afnbooitart Method mumixaM lang i.Sx a oMetmiT tnemecnahnE sda esBsalG 6.3 .ni m2 suhotercaaEmotaiD 4.8 .ni m21 muinommalyhtemirtlyteC 7.4 C° 0t5a.n i0m21 edimorb raguS/dic AonimA 5.8 . . Ttn0.ai8Rm1 enimalycedoD 20.5 t.an i2m C° 08 gnilioB 18.3 C°0 0 t1.an i5m1 ssa lhgt inwoitacin o,snoitacin oes rdaetpmet teav a ehswna elmacinahc eem hgtnomA . gnita edh n,ahtr aseuoecamota idd ndaa esbsa lhgt igwnika h,s) m0n 0d2 n ma0n 4s(daeb si aswda esbsa lhgt igwnika hds nnaoitacinoS n sees ehaswtletgre obd pe h sdoseontuhdertntcen sefthoet cpar f asenhehosrtopso dednheetda rhbt arsaueoecamot ah itgdi nw.isk daaheSb .e hrst uur dtoae aee tn rct rieeaIsnohtmaeptioheeltbraid 1,1 0 r5so1efropso dgnneil ito abht tcartx elli wsetunim tuoht irhwot inwoitacin otsa hdtnu o ef.v maeeh Whmto ra fpe dhfl tola gi Wan inoitati g .Aselpma sru on oevitceffen isa wsdae bssalg - L - rsoda esbsa lhgt igwuB .y lneOvitcep s,e 8 rd .3rnf 4.o aoa6tyd pcbeadatfcar t exdheetsaer chnti rsaueoecamotaid feo 0d1eriuq ehrtr aseuoecamota ie dhhtt isweitluciff iedht - gni erbet feam igtniltt e fssoetun i5m1 .n oisne p e sefltuhop cstmaa ar r stoe dxtdneeritoatiga xa i omst atwsr i ef. haT pedr ogmnitcart x fesona elmacime hecer hdtenima xoes leaW noitulos eropsod nleairetc aeb h fteolpm ahast i)wBAT Ce(dimo rmbuinommalyhtemirtlyt e fco dex i,mslavret neim itt e tsdaevom eerr eswelp m. aCS° 0 o5etrutx iem htta edh nnaoisnepsus ko odtoht e sm.i dheTtcell oncoissi meecnecsenimuloto he phd tn,anoitul obhsTtiw two hours to xa oodctetpmet t oa.s 4l e .afWr7oot c yaa abpfe dhdtesaerc nd ineasael emrumix as mthicaer e h ftlo l fanooislup xee h stsiseco rtpa h ntpie ttssr ief hetcn i,sgnitanimr eogt nsieropsod neeht dehsilpmoc cs aa sw.i ehrTopsod ne ehmto rafpd dn a,enigaraps a,eninal af oerutxi magnidd ay b e hgtnitcell odc n,aslavret n tiganilpm a,snoisneps uesropsod nee h fteolpm aoaestsoculg d nnaoitartnecn oac pldaiti nei hstem i8t .d5esael edroht esm i. hnToissi meecnecsenimulotohp took three hours. Lat e re prsaeropsod ne ehhtci hn,wistnemirep xree - deveih ceav a,hdetaeh enina lea h rteono leanina lraeht ihet iswero pes hmto raf p fdeosael eertelpm otcsomla - enigarapsa - loev emlumix ae mhhtca eosrtr u oso3trh u do2ehriuq elrli ttt siu,bliatkc oecsoculg f lacime htcs a.e lsheTt aersael eerlbatpec cndaietlus esrdoht eemse hfrtoeh t.i eesNael earpd s i. hsTnoisneps usseropsod n oe)tAD De(nimalyced o fdnooitid d sadienima xeev a ehdwohtem l bsa aAw D eD. hnToitid dBaA TeC h otrtenn armalim i nasein osdaw a pe dhfl toleasael eoert esael ear p ed. hCT° 0 t8saetun i n2msieropsod nleairetc aeb hmto r)fgnili o ybdbenimret esda( eropsod neevital efnrooitcn ugfno r sAt aiDshDtiw - to - .eru tnao ridetnpaamrett nAeDcDnoc 11 ehca e srAi D fDloev edllohser heatcnO eropso dtfnnoeednepe desnsiiae l aeef pt roeda,hrdt - to - ,yl r.at ecleC pdxle u eo,snweaorutarep mnfeootit css neaausfaer con s iel. tao eaihr tTAaDrD .seropsod nleairetc ambo ra fpedr ogmnisael er rodfoht etms ee b hsntioitid dAaDD DISCUSSION. The ts oe mhsttimr esperopsod ne ehmto ra fpe dhfl tolfeaosaeler nr u undoo e.s eauBqinhc esti hhtt isweropsod nleairetc afnbooitcet eedlbiss oepvitisnes - 5 r onfoisneps u fsLom/U F0C00, 3 f8nooitcet e fdtoim idlecnahne B. globigii , 11 s stecle t neeadwc naht 000, 0n1a hsts edletcet eydllatnemirep xeev a. eheWsael eAr DhDt iLwm/U F0C005 lang ihast itwnemtae rAt DeD hgtni s Gu fBLom/UFC - to - gnitacid n,ni6a hrtetae rfogoit aersion t esdtimr enpoitcet e fdloev es li. hLTm/U F0C0 0n5a hsts e ellbl iDw OrL utoaht noitce ta eht 01 tnega 00 5eh tt agnilpma sf osetuni m0 1nah -tsse ln ileve lri af oreti lre pelcitra pgniniatnoc e rnaoitcell ofecot asri hhtt iswrelpm alsaicre m .m oLon1tmCoitartnecn od cneat aertunim/L itcete devitisne sylemertx egnittimre pelbaliava .e rehpso memt hoastrefropso dlnaeire t ncfoaob yawg u,Dgnisseco r opdn nmaets yescnecserou llfaicremm ohactiW B. globigii nee bsa h .Lm/ U0F 0Clf9 eo1dt veaaetlceted 12 si hhtca e or sttuimr eyp ammets yrs u nositnemevor p mI ht idw nnaoitcet e fdloevel . Lm/U F0 C0n2a hsts efslotim inloitcet ehdca etrnemtae rA tDeDht mo rgfnilpm a fsyotilibicudorp eer h steiuqinhc esti h fteo seuvitatitna u oqntrecn o fcaoe reanO selpm aestacilp err onfoitaiv eddradna tesvital e%r 5 na1sitlus esr i. hnToisneps uas 6 d na stimi l ll e sweiuqinhc ert u,onoitatim isli hettip s. edDoht eem h fteo seuvitatitnauq - detius ot esu sa a .mets ygsninr atwre ltasrif SNOISULCNOC r odfoht erm u foyotivitisn ehsg ieh hettacid nyilrae ldcetrop eerv a ehswtlus eerhT eropso dlnaeiretcab sneiropso dLfnmoe/ U0F 0Cn0 a5sh sttec le . teno el otedbeiraWtace ted sdioht e.r msueOropsod ne ehmto ra f pfedosael edrecnah nr eoA fDhDt iswetu n ni5am hstsel lC l fAeo seu hhtguo r. hsTesnops eervitis oepsl amfo refnummi etahpso hy pnxaelpm ooct 3 .euqinhce tsih thti wecnerefretn itnacifingi syln oeh tdetanimil eeva he w,elpma san itneserp tsri fro ftneiciffu snah tero ms it i,detimi ls idohte meh tf oes uevitatitnau qelihW - t.reeslua STNEMEGDELWONKCA loasre veegsdelwo n hk oscstiarwoh teuhaT .k rs oirtwhortfop pdsunesal p smfeaocsru omalareG ieDirr a. Cs,Mtnatsis shacraes erremm ur suftoorof fe ehetzingoc eoertk idllu oewW r efedhob etmamurnof ro,eh)pw0 0,0929 9y1l (la el.,KostLMned uStR sArd,Tun)Soa0002( .er edhebircs esdtnemirepxe dal eohnirgell elPu a.P reDli hdwemrofr esp akwr oswi h fteo m oS licnu ohCcraes elRanoitaN - . rd Dnpaihsetaicos shAcraes eyRrotarob ahLcraes eyRm r.AS.U noitacud Egnireenign Ero fyteico Snacirem An adle hlle FsalohciN - hcraes eyRm r.AS.U Laboratory Postdoc lacime hdCooweg dyEm r.AS .eU hktna hot s .l epaWihswoll elFarot selpm aasiretc aeb h fteom ogsnidivo rrp o,fonret aePitt o.D syMllaicep s,eretn elCacigoloiB yawg ufnDoitr an Ma. DrfeDocnatsis se ahettaicerp pe,awnoit i.d ydndaIu tssi hndtiesu gnivorP Gr Buntoinese rspeitirup me ihfntooitisopm oe chfstonoitpircs es dir ho, fT,Udnuo rG snoissucs ird udo n fasonoitsegg ulsufpl eeh hetgdelwonk c oaetk idllu o ew,wylla n. isFelpmas aiti no ih,wyrotarob ahLcraes eyRm r.AS .e Uhfntoes o.dRLiv a. DrhDtiw sih tdepoleve dyll .dohtem 6 REFERENCES .1 .G .dWl udonGa ,.tAsruH er olpaSiret ceahBT ., )s,cw9skie6ermN9roe1 PYdacA( .2 ,nosdrah c.iSR.F Chem. Rev. 82 , 541 (1982) . .3 ,nosd r,d arn. ,h eaF.rc. vSeiM.i dRSr, nDf. elJ.l aPo. cBD.tHeM .J .syhP.mehC 97 , 553 ( 1993) . .4 ,eipsell i ..GdB Jn,anes o ..R,LD. r,Jll e ..F,FNonirgell e..PMP Anal. Chem. 70(9) , 1755 (1998). .5 D. L. Rosen, U.S. Patent 5,876,960, 1999. .6 ,eipsell i . G.BdJ n,aonirgell e . P.M,P. r,Jll e .F.FN Anal. Chim. Acta 426(1) , 43 - 50 (2001) . .7 T. D. Ba ,yrre h..dS DAnaaler Anal. Biochem. 71 , 351 - 357 (1976). .8 .noitacinumm oectavi r, pT,Udnuo rgGnivo ryPawg uyDm r.AS .,Unitr alMein a.DrD .9 R. H. Petrucci, ,noitacilpp Anredo Mdn aselpicnir Pyrtsimeh ClareneG 4 ht ,nallimca M,.d e New York, 1985, p 659. .01 .L .JR ode and J. W. Foster, Archiv für Mikrobiologie 36 , 67 - 94 (1960). .11 .noitarape r nptipircsun a,ms tlus edrehsilbup n,uonirgell e . P.MdP n.a r,Jll e.FF.N .21 .stlus edrehsilbup n,uyt isrevi nnUots o,Bvell u .VdV nLa R,A. r,Jll e.FF.N 7
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