Correlative light and electron microscopy. 2 PDF
Preview Correlative light and electron microscopy. 2
Series Editors Leslie Wilson Department of Molecular, Cellular and Developmental Biology University of California Santa Barbara, California Phong Tran University of Pennsylvania Philadelphia, USA & Institut Curie, Paris, France AcademicPressisanimprintofElsevier 32JamestownRoad,LondonNW17BY,UK 525BStreet,Suite1800,SanDiego,CA92101-4495,USA 225WymanStreet,Waltham,MA02451,USA TheBoulevard,LangfordLane,Kidlington,OxfordOX51GB,UK Firstedition2014 Copyright#2014,ElsevierInc.AllRightsReserved Nopartofthispublicationmaybereproducedortransmittedinanyformorbyanymeans, electronicormechanical,includingphotocopying,recording,oranyinformationstorageand retrievalsystem,withoutpermissioninwritingfromthepublisher.Detailsonhowtoseek permission,furtherinformationaboutthePublisher’spermissionspoliciesandour arrangementswithorganizationssuchastheCopyrightClearanceCenterandtheCopyright LicensingAgency,canbefoundatourwebsite:www.elsevier.com/permissions. Thisbookandtheindividualcontributionscontainedinitareprotectedundercopyrightbythe Publisher(otherthanasmaybenotedherein). Notices Knowledgeandbestpracticeinthisfieldareconstantlychanging.Asnewresearchand experiencebroadenourunderstanding,changesinresearchmethods,professionalpractices,or medicaltreatmentmaybecomenecessary. Practitionersandresearchersmustalwaysrelyontheirownexperienceandknowledgein evaluatingandusinganyinformation,methods,compounds,orexperimentsdescribedherein. Inusingsuchinformationormethodstheyshouldbemindfuloftheirownsafetyandthesafety ofothers,includingpartiesforwhomtheyhaveaprofessionalresponsibility. Tothefullestextentofthelaw,neitherthePublishernortheauthors,contributors,oreditors, assumeanyliabilityforanyinjuryand/ordamagetopersonsorpropertyasamatterofproducts liability,negligenceorotherwise,orfromanyuseoroperationofanymethods,products, instructions,orideascontainedinthematerialherein. ISBN:978-0-12-801075-4 ISSN:0091-679X ForinformationonallAcademicPresspublications visitourwebsiteatstore.elsevier.com Contributors Alin Achim Visual Information Laboratory, University ofBristol, Bristol, UnitedKingdom Claude Antony EuropeanMolecular Biology Laboratory,Meyerhofstrasse 1, Heidelberg, Germany;InstitutdeGe´ne´tiqueetBiologieMole´culaireetCellulaire,1rueLaurent Fries,BP10142,andDepartmentofStructuralBiologyandGenomics,Institutde Ge´ne´tique etde Biologie Mole´culaire etCellulaire, INSERM, U964, CNRS UMR7104, Illkirch, France Vera Bandmann Biomineralization,INM-LeibnizInstituteforNewMaterials,Saarbru¨cken,Germany SebastianBanhart Junior ResearchGroup Sexually Transmitted Bacterial Pathogens (NG 5), Robert Koch Institute,Berlin, Germany NorbertBannert Centre for HIVand other Retroviruses (FG 18), Robert Koch Institute, Berlin, Germany Derron Bishop Department ofPhysiology,IndianaUniversity School of Medicine-Muncie, Muncie,Indiana,USA Erik Bos Department ofMolecular Cell Biology, Section Electron Microscopy, Leiden University MedicalCenter,Leiden, The Netherlands FilipBraet SchoolofMedicalSciences (Discipline ofAnatomyand Histology)—The Bosch Institute;Charles Perkins Centre,and Australian Centre for Microscopy & Microanalysis,The University ofSydney, Sydney, NSW,Australia Robert J. Bryson-Richardson SchoolofBiologicalSciences, Faculty ofsciences, Monash University, Melbourne, Victoria,Australia RaffaellaCarzaniga Electron Microscopy Unit, London ResearchInstitute,CancerResearchUK, London,United Kingdom KatherineCeller MolecularBiotechnology, InstituteofBiologyLeiden, Leiden University, POBox 9505, Leiden, The Netherlands Delfine Cheng SchoolofMedicalSciences (Discipline ofAnatomyand Histology)—The Bosch Institute,The University ofSydney, Sydney, NSW,Australia xi xii Contributors Lucy M. Collinson ElectronMicroscopyUnit, London Research Institute, Cancer Research UK, London,UnitedKingdom Nielsde Jonge Innovative Electron Microscopy, INM-Leibniz Institutefor New Materials, Saarbru¨cken,Germany Ce´dric Delevoye Centre de Recherche, Institut Curie,and Structure and Membrane Compartments,CNRSUMR144, Paris, France KyleC.Dent Diamond Light Source, Harwell Science and Innovation Campus, Didcot,and Oxford Particle Imaging Centre,Divisionof StructuralBiology, WellcomeTrust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom Marie-CharlotteDomart ElectronMicroscopyUnit, London Research Institute, Cancer Research UK, London,UnitedKingdom Elizabeth Duke Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire, United Kingdom Jeroen C.Eikenboom Department ofThrombosis and Hemostasis, Einthoven Laboratory for Experimental VascularMedicine, Leiden University Medical Center,Leiden, The Netherlands Frank G.A. Faas Department ofMolecularCellBiology, Section Electron Microscopy, Leiden University Medical Center, Leiden, The Netherlands IsabelFerna´ndez deCastro CellStructureLaboratory,CentroNacionaldeBiotecnologı´a,ConsejoSuperiorde InvestigacionesCientı´ficas(CNB-CSIC), Cantoblanco,Madrid, Spain Pascal Fua Computer Vision Laboratory, EPFL,Lausanne,Switzerland Kimberley H.Gibson Max Planck Institute ofMolecular Cell Biology and Genetics, Dresden, Germany KayGru¨newald Oxford Particle Imaging Centre,Divisionof StructuralBiology, WellcomeTrust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom Christoph Hagen Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom Contributors xiii XavierHeiligenstein EuropeanMolecular Biology Laboratory,Meyerhofstrasse 1, Heidelberg, Germany; InstitutCurie,Centre de Recherche, and Structure and Membrane Compartments,CNRS UMR144, Paris, France JeffreyHenriquez SchoolofMedicalSciences (Discipline ofAnatomyand Histology)—The Bosch Institute,The University ofSydney, Sydney, NSW,Australia Lorna Hodgson SchoolofBiochemistry,University ofBristol, Bristol, United Kingdom Anthony Holtmaat Department ofNeuroscience,University MedicalCenter(CMU), University of Geneva, Geneva, Switzerland Jacob P.Hoogenboom DepartmentofImagingPhysics,FacultyofAppliedSciences,DelftUniversityof Technology, Delft, The Netherlands William A. Horton Research Department, ShrinersHospitalfor Children, Portland, Oregon,USA Ilse Hurbain Centre deRecherche, InstitutCurie;Structure and MembraneCompartments, CNRS UMR144, and Cell and TissueImaging Facility(PICT-IBiSA), CNRS UMR144, Paris, France Minh Huynh Australian Centre for Microscopy &Microanalysis, The University of Sydney, Sydney, NSW, Australia Anne Jorstad ComputerVision Laboratory, EPFL,Lausanne, Switzerland Douglas R.Keene Research Department, ShrinersHospitalfor Children, Portland, Oregon,USA Martin Kerschensteiner Research Unit Therapy Development,InstituteofClinical Neuroimmunology, Ludwig-Maximilian University, and Munich Center for Systems Neurology (SyNergy), Nordallee, Munich,Germany Graham W. Knott BioEM Facility, Centre ofElectron Microscopy, EPFL,Lausanne,Switzerland Roman I.Koning Department ofMolecular Cell Biology, Section Electron Microscopy, Leiden University MedicalCenter,Leiden, The Netherlands Abraham J. Koster Department ofMolecular Cell Biology, Section Electron Microscopy, Leiden University MedicalCenter,Leiden, The Netherlands xiv Contributors MarkoLampe European MolecularBiologyLaboratory,and Department ofTranslational Pulmonology, Translational LungResearchCenter (TLRC), University of Heidelberg, Heidelberg, Germany Michael Laue AdvancedLightandElectronMicroscopy(ZBS4),RobertKochInstitute,Berlin, Germany NalanLiv DepartmentofImagingPhysics,FacultyofAppliedSciences,DelftUniversityof Technology, Delft, The Netherlands Bohumil Maco BioEMFacility,CentreofElectronMicroscopy,andComputerVisionLaboratory, EPFL,Lausanne, Switzerland Kazimierz Madela AdvancedLightandElectronMicroscopy(ZBS4),RobertKochInstitute,Berlin, Germany JudithMantell Wolfson Bioimaging Facility, University ofBristol, Bristol, UnitedKingdom Jana Meissner MaxPlanckInstituteofMolecularCellBiologyandGenetics,Dresden,Germany Thomas Misgeld MunichCenter for Systems Neurology (SyNergy), and InstituteofNeuronalCell Biology, Technische Universita¨tMu¨nchen,Nordallee,Munich,Germany MarjonJ. Mourik Department ofMolecularCellBiology, Section Electron Microscopy, Leiden University Medical Center, Leiden, The Netherlands David Nam Visual Information Laboratory, University ofBristol, Bristol, United Kingdom Ivana Nikic ResearchUnitTherapy Development, Instituteof Clinical Neuroimmunology, Ludwig-Maximilian University, Munich,Nordallee, Germany ViolaM.J.Oorschot Monash Micro Imaging,Monash University, Melbourne, Victoria, Australia Benjamin S. Padman DepartmentofBiochemistryandMolecularBiology,andMonashMicroImaging, Monash University, Melbourne, Victoria,Australia Diana B.Peckys Innovative Electron Microscopy, INM-Leibniz Institutefor New Materials, Saarbru¨cken,Germany Contributors xv Christopher J.Peddie Electron Microscopy Unit, London ResearchInstitute,CancerResearchUK, London,United Kingdom InaPeristere EuropeanMolecularBiologyLaboratory,Meyerhofstrasse1,Heidelberg,Germany Janett Piesker AdvancedLightandElectronMicroscopy(ZBS4),RobertKochInstitute,Berlin, Germany Georg Ramm DepartmentofBiochemistryandMolecularBiology,andMonashMicroImaging, Monash University, Melbourne, Victoria, Australia Graca Raposo Centre deRecherche, InstitutCurie;Structure and MembraneCompartments, andCellandTissueImagingFacility(PICT-IBiSA),CNRSUMR144,Paris,France Cristina Risco CellStructureLaboratory,CentroNacionaldeBiotecnologı´a,ConsejoSuperiorde InvestigacionesCientı´ficas (CNB-CSIC),Cantoblanco, Madrid, Spain Lynn Y.Sakai Research Department, ShrinersHospitalfor Children, Portland, Oregon,USA Jean Salamero Centre deRecherche, InstitutCurie;Cell and TissueImaging Facility (PICT-IBiSA), and Spatio-Temporal Modeling Imaging and Cellular Dynamics, CNRS UMR144, Paris, France Laura Sanz-Sa´nchez CellStructureLaboratory,CentroNacionaldeBiotecnologı´a,ConsejoSuperiorde InvestigacionesCientı´ficas (CNB-CSIC),Cantoblanco, Madrid, Spain Gerry Shami SchoolofMedicalSciences (Discipline ofAnatomyand Histology)—The Bosch Institute,The University ofSydney, Sydney, NSW,Australia NicholasR. Smith Department ofCelland Developmental Biology, Oregon Health Sciences University, Portland, Oregon, USA TamarE.Sztal SchoolofBiologicalSciences, Faculty ofsciences, Monash University, Melbourne, Victoria,Australia Erin M. Tranfield Instituto Gulbenkian de Cieˆncia, Rua da Quinta Grande 6, Oeiras, Portugal, and European MolecularBiologyLaboratory, Meyerhofstrasse 1, Heidelberg, Germany xvi Contributors SaraL. Tufa ResearchDepartment,ShrinersHospital for Children, Portland, Oregon, USA Jack A.Valentijn Department ofMolecularCellBiology, Section Electron Microscopy, Leiden University Medical Center, Leiden, The Netherlands Karine M.Valentijn Department ofMolecularCellBiology, Section Electron Microscopy, Leiden University Medical Center, Leiden, The Netherlands Gilles P.van Wezel MolecularBiotechnology,InstituteofBiology Leiden, Leiden University,PO Box 9505, Leiden, The Netherlands Jean-Marc Verbavatz MaxPlanckInstituteofMolecularCellBiologyandGenetics,Dresden,Germany PaulVerkade Wolfson Bioimaging Facility, Schools ofBiochemistry and Physiology & Pharmacology, University of Bristol, Bristol, United Kingdom Daniela Vorkel MaxPlanckInstituteofMolecularCellBiologyandGenetics,Dresden,Germany Joost Willemse MolecularBiotechnology,InstituteofBiology Leiden, Leiden University,PO Box 9505, Leiden, The Netherlands Melissa H.Wong Department ofCell and DevelopmentalBiology, OregonHealthSciences University, Portland, Oregon, USA AnjaZimmermann Centre for HIV and other Retroviruses (FG 18), RobertKoch Institute,Berlin, Germany Preface Idonotsearch,Ifind. (PabloPicasso) Withinarathershortperiodoftime,wepresentasecondMCBvolumeonCorrelative Light and Electron Microscopy (CLEM). Immediately after the completion of the first volume, we realized that numerous additional new as well as established approaches—notmentionedinthefirstvolume—neededtobepresentedsothatin- terestedcolleaguescouldfollowthesedetailedandwell-establishedprotocols.Itwas alsobypopulardemandthatwedecidedtolookfortechniquesandapproachesthat werenotpublishedinthefirstvolume.Weareawareofthefactthatalsothesecond bookonCLEMtechniquesisbynomeansexhaustive.However,wehopethatthis secondvolume—aswellasthefirstone—willserveasaninspiringsourceforfurther research involving the combination ofimaging modalities. Since the publication of the first book, the motivation to apply correlative approachescontinuestobestrong.MotivationsforCLEMincludelocatingandim- aging certain “spots” in cells and tissues, collecting dynamic as well as structural information about the same specimen, and/or simply increasing sample size and throughput. Certainly, we all wish to “find” without extensive “searching.” What- everthemotivationsareforeachindividualresearcher,itisalwaysthecombination ofusingcomplementingimagingtechniquesthatisexpectedtodelivermorequan- titativeandqualitativeinformationaboutagivenspecimenasopposedtojustusing one ofthe applied techniques. MostofthecurrentactivitiesinCLEMpresentedinthisbookarecenteredaround threekeytopics.First,itisthedevelopmentofCLEMprobesthatattractsthemost attentioninthefield.Probesthatallowthedetectionofalabeledstructurebyfluores- cenceandelectronmicroscopyaredesperatelyneeded.Second,muchresearchhas beendoneonthepreservationoffluorescence,suchasGFP,inplastic-embeddedsam- plesforelectronmicroscopy.Again,theseactivitiesaremainlydrivenbytheneedto correlatespecificsignalswithcellularultrastructure.Third,itistheanalysisofthecor- relativedatathatcomesmoreandmoreintofocus.Automatedregistrationalgorithms willbeincreasinglyimportanttoaccuratelycorrelateLMandEMdata.Allthetech- niquespresentedinthisbook“fall”intooneormoreofthesethreekeytopics. Inparalleltothissecondvolume,wewillcontinuetoteachtechniquesforCLEM duringabiannualpracticalcoursesponsoredbyEMBO.Itiscertainlyourcontinued hopethatboththepracticalcourseandthispublicationwillhelptofurthermotivate crosstalk andcollaboration between all imaging worlds. Thomas Mu¨ller-Reichertand PaulVerkade Dresdenand Bristol July 1, 2014 xvii xviii Preface ACKNOWLEDGMENTS TheauthorswouldliketothankSarahLay(Elsevier)forherhelpinbringingthissecondvol- umeonCorrelativeLightandElectronMicroscopytocompletion.
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