Cell Reprogramming: Methods and Protocols

270 Pages·2015·8.905 MB·English

by  Paul J. Verma, Huseyin Sumer (eds.)

Methods in
Molecular Biology 1330
Paul J. Verma
Huseyin Sumer Editors
Cell Re pro-
gramming
Methods and Protocols
M M B
ETHODS IN OLECULAR IOLOGY
Series Editor
John M. Walker
School of Life and Medical Sciences
University of Hertfordshire
Hatfield, Hertfordshire , AL10 9AB, UK
For further volumes:
http://www.springer.com/series/7651

Cell Reprogramming
Methods and Protocols
Edited by
Paul J. Verma
Stem Cell and Genetic Engineering Group, Department of Materials Engineering,
Faculty of Engineering, Monash University, Clayton, VIC, Australia; South Australian Research &
Development Institute (SARDI), Turretfield Research Centre, Rosedale, SA, Australia
Huseyin Sumer
Swinburne University of Technology, Hawthorn, VIC, Australia
Editors
Paul J . V erma Huseyin S umer
Stem Cell and Genetic Engineering Group Swinburne University of Technology
Department of Materials Engineering Hawthorn, VIC, Australia
Faculty of Engineering
Monash University
Clayton , V IC , Australia
South Australian Research & Development
Institute (SARDI)
Turretfield Research Centre
Rosedale, SA, Australia
ISSN 1064-3745 ISSN 1940-6029 (electronic)
Methods in Molecular Biology
ISBN 978-1-4939-2847-7 ISBN 978-1-4939-2848-4 (eBook)
DOI 10.1007/978-1-4939-2848-4
Library of Congress Control Number: 2015955417
Springer New York Heidelberg Dordrecht London
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Prefa ce
Cell Reprogramming: Methods and Protocols is a comprehensive review of cellular repro-
gramming technology in vertebrates, aimed at reprogramming differentiated cells and germ
line transmission of pluripotent stem cells. The emphasis here is on providing readily repro-
ducible techniques for inducing pluripotency in somatic cells for disease modeling and the
generation of cloned embryos and animals in a number of key research and commercially
important species. Additional chapters dealing with such reprogramming-related issues
such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of repro-
gramming intermediates are also included. A section providing alternative cutting-edge
methods for nuclear transfer, as well as techniques for the production of germ line chimeras
from embryonic stem cells and induced pluripotent stem cells is also incorporated. This is
complimented with the neonatal care and management of somatic cell nuclear transfer
derived offspring.
Cell Reprogramming also provides an understanding of the factors involved in nuclear
reprogramming, which is imperative for the success of reprogramming. This volume will
prove benefi cial to molecular biologists, stem cell biologists, clinicians, biotechnologists,
students, veterinarians, and animal care technicians involved with reprogramming, nuclear
transfer, and transgenesis.
Clayton, VIC, Australia P aul J. V erma
Hawthorn, VIC, Australia Huseyin S umer
v

Contents
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i x
PART I BACKGROUND
1 Cellular Reprogramming in Basic and Applied Biomedicine:
The Dawn of Regenerative Medicine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Wendy Dean
PART II DE NOVO REPROGRAMMING
2 Synthetic mRNA Reprogramming of Human Fibroblast Cells. . . . . . . . . . . . . 1 7
Jun L iu and P aul J . V erma
3 M icroRNA-Mediated Reprogramming of Somatic Cells into
Induced Pluripotent Stem Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Shelley E.S. Sandmaier and Bhanu Prakash V.L. Telugu
4 G eneration of Footprint-Free Induced Pluripotent Stem Cells
from Human Fibroblasts Using Episomal Plasmid Vectors. . . . . . . . . . . . . . . . 3 7
Dmitry A. Ovchinnikov , Jane Sun , and Ernst J. Wolvetang
5 R eprogramming of Human Fibroblasts with Non-i ntegrating RNA
Virus on Feeder-Free or Xeno-Free Conditions. . . . . . . . . . . . . . . . . . . . . . . . 4 7
Pauline T. L ieu
PART III LIVESTOCK, DOMESTIC AND ENDANGERED SPECIES
6 Inducing Pluripotency in Cattle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 7
Luis F. Malaver-Ortega , Amir Taheri-Ghahfarokhi ,
and Huseyin S umer
7 G eneration of Induced Pluripotent Stem Cells (iPSCs) from Adult
Canine Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Sehwon K oh and J orge A . Piedrahita
8 Derivation of Equine-Induced Pluripotent Stem Cell Lines
Using a piggyBac Transposon Delivery System and Temporal
Control of Transgene Expression . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Kristina Nagy and Andras N agy
9 G eneration of Avian Induced Pluripotent Stem Cells. . . . . . . . . . . . . . . . . . . . 89
Yangqing Lu , F ranklin D . W est , Brian J . Jordan , R obert B. B eckstead ,
Erin T. J ordan , and Steven L . Stice
vii
viii Contents
10 Generation of Induced Pluripotent Stem Cells from Mammalian
Endangered Species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 01
Inbar Friedrich B en-Nun , S usanne C . M ontague , M arlys L . H ouck ,
Oliver Ryder , and Jeanne F . L oring
PART IV GERM-LINE TRANSMISSION OF PLURIPOTENT STEM CELLS
11 Generation of Efficient Germ-Line Chimeras Using Embryonic
Stem Cell Injection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
William A . R itchie
12 Generation of Viable Mice from Induced Pluripotent
Stem Cells (iPSCs) Through Tetraploid Complementation . . . . . . . . . . . . . . . 1 25
Lan K ang and S haorong G ao
13 C loning Endangered Felids by Interspecies Somatic Cell Nuclear Transfer. . . . 1 33
Martha C. G ómez and C . Earle P ope
14 Generation of Chimeras from Porcine Induced Pluripotent Stem Cells . . . . . . 1 53
Franklin D. W est , Steve L . T erlouw , John R. Dobrinsky ,
Yangqing Lu , E rin T. J ordan , and Steven L. Stice
15 A Novel Method of Somatic Cell Nuclear Transfer
with Minimum Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 69
S.M. Hosseini, F. Moulavi, and M.H. Nasr-Esfahani
16 N eonatal Care and Management of Foals Derived by Somatic
Cell Nuclear Transfer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 89
Aime K . J ohnson and Katrin Hinrichs
PART V INFLUENCING REPROGRAMMING AND GENOME EDITING
17 Isolation of Reprogramming Intermediates During Generation
of Induced Pluripotent Stem Cells from Mouse Embryonic Fibroblasts. . . . . . 205
Christian M . N efzger , S ara A laei , and Jose M. Polo
18 Analysis of Mitochondrial DNA in Induced Pluripotent
and Embryonic Stem Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 19
William Lee, Richard D.W. Kelly, Ka Yu Yeung, Gael Cagnone,
Matthew McKenzie, and Justin C. St. John
19 Genome Modification of Pluripotent Cells by Using Transcription
Activator-Like Effector Nucleases (TALENs). . . . . . . . . . . . . . . . . . . . . . . . . . 253
Amir Taheri-Ghahfarokhi , Luis F. Malaver-Ortega ,
and Huseyin Sumer
Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 269
Contributors
SARA ALAEI • Department of Anatomy and Developmental Biology,
Australian Regenerative Medicine Institute, M onash University , Clayton,
VIC, A ustralia
ROBERT B. B ECKSTEAD • Department of Animal and Dairy Science,
Regenerative Bioscience Center , U niversity of Georgia , Athens, G A , USA
INBAR F RIEDRICH BEN-NUN • Department of Chemical Physiology, Center for
Regenerative Medicine, T he Scripps Research Institute , L a Jolla , C A , U SA
GAEL C AGNONE • The Mitochondrial Genetics Group, Centre for Genetic Diseases,
Hudson Institute of Medical Research , Monash University , C layton , V IC , A ustralia
WENDY DEAN • Epigenetics Programme, The Babraham Institute , Cambridgeshire, U K
JOHN R . DOBRINSKY • JRD Biotechnology , Oregon , W I, USA
SHAORONG G AO • National Institute of Biological Sciences, NIBS , B eijing,
People’s Republic of China ; S chool of Life Sciences and Technology , T ongji University ,
Shanghai, P eople’s Republic of China
MARTHA C . GÓMEZ • Audubon Nature Center for Research of Endangered Species ,
New Orleans, L A , U SA
KATRIN HINRICHS • Department of Veterinary Physiology and Pharmacology,
College of Veterinary Medicine and Biomedical Sciences, T exas A&M University ,
College Station, T X , U SA
S. M. HOSSEINI • Department of Reproductive Biotechnology at Reproductive Biomedicine
Research Center , Royan Institute for Biotechnology, ACECR , Isfahan, I ran
MARLYS L . HOUCK • San Diego Zoo Institute for Conservation Research , E scondido,
CA , U SA
JUSTIN C. ST. JOHN • The Mitochondrial Genetics Group, Centre for Genetic Diseases,
Hudson Institute of Medical Research , Monash University , C layton , V IC , A ustralia
AIME K. J OHNSON • JT Vaughn Large Animal Teaching Hospital, College of Veterinary
Medicine, A uburn University , A uburn, A L, U SA
BRIAN J . J ORDAN • Department of Animal and Dairy Science, Regenerative Bioscience
Center , U niversity of Georgia , A thens , GA , U SA
ERIN T. J ORDAN • Department of Animal and Dairy Science, Regenerative Bioscience
Center , U niversity of Georgia , Athens , G A , USA
LAN KANG • Institute of Cancer Stem Cell, Dalian Medical University , D alian,
People’s Republic of China ; N ational Institute of Biological Sciences, NIBS , B eijing,
People’s Republic of China
RICHARD D. W. KELLY • The Mitochondrial Genetics Group, Centre for Genetic Diseases,
Hudson Institute of Medical Research , Monash University , C layton , V IC , A ustralia
SEHWON K OH • Department of Cell Biology, Duke University , D urham , N C, U SA ;
Duke University Medical Center, D uke University , D urham , N C, U SA
WILLIAM LEE • The Mitochondrial Genetics Group, Centre for Genetic Diseases, Hudson
Institute of Medical Research , Monash University , C layton, VIC, Australia
ix