Antimicrobial Susceptibility Testing Disk Diffusion & Detection of Antimicrobial Resistance Babak Valizadeh , DCLS [email protected] 1389 / 07 / 08 2010.09.30 1 2 CLSI 2006 – 2010 3 Antimicrobial Susceptibility Testing CLSI : Clinical and Laboratory Standards Institute CLSI (2005) , formerly NCCLS (2004) NCCLS :National Committee for Clinical Laboratory Standards Antimicrobial Susceptibility Testing (AST) vs. Antibiogram Susceptible vs. Sensitive 4 Antimicrobial Resistance 5 Antimicrobial Resistance 6 Antimicrobial Resistance 7 Antimicrobial Resistance 8 Proposed definitions of MDR/XDR/PDR bacteria MDR : Multidrug Resistant Bacteria XDR : Extensively Drug Resistant Bacteria PDR : Pan Drug Resistant Bacteria 9 Automated System Automated bacterial identification and Antimicrobial susceptibility testing (ID/AST) system High-Quality MIC & Extended MIC ranges VITEK® 2 –bioMérieux Microscan-Dade Phoenix - BD 10
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