Antibody Expression and Production PDF
Preview Antibody Expression and Production
Antibody Expression and Production Cell Engineering Volume7 SeriesEditor ProfessorMohamedAl-Rubeai UCDSchoolofChemicalandBioprocessEngineering UniversityCollegeDublin Dublin,Ireland EditorialBoard DrHansjorgHauser HelmholtzCentreforInfectionResearch Braunschweig Germany ProfessorMichaelBetenbaugh JohnsHopkinsUniversity Baltimore,USA ProfessorMartinFussenegger SwissFederalInstituteofTechnology Zurich,Switzerland ProfessorNigelJenkins NationalInstituteforBioprocessingResearchandTraining Dublin,Ireland DrOtto-WilhelmMerten A.F.M.-Genethon11 GeneTherapyProgram Evry,France Forothertitlespublishedinthisseries,goto http://www.springer.com/series/5728 CELL ENGINEERING Vol. 7: Antibody Expression and Production Editedby MohamedAl-Rubeai UniversityCollegeDublin,Ireland 123 Editor Prof.MohamedAl-Rubeai SchoolofChemicalandBioprocess Engineering UniversityCollegeDublin Dublin Ireland [email protected] ISSN1389-6946 ISBN978-94-007-1256-0 e-ISBN978-94-007-1257-7 DOI10.1007/978-94-007-1257-7 SpringerDordrechtHeidelbergLondonNewYork LibraryofCongressControlNumber:2011928367 ©SpringerScience+BusinessMediaB.V.2011 Nopartofthisworkmaybereproduced,storedinaretrievalsystem,ortransmittedinanyformorby anymeans,electronic,mechanical,photocopying,microfilming,recordingorotherwise,withoutwritten permissionfromthePublisher,withtheexceptionofanymaterialsuppliedspecificallyforthepurpose ofbeingenteredandexecutedonacomputersystem,forexclusiveusebythepurchaserofthework. Printedonacid-freepaper SpringerispartofSpringerScience+BusinessMedia(www.springer.com) Contents 1 ExpressionofAntibodyinMammalianCells. . . . . . . . . . . . . 1 ThomasJostock 2 Bioreactor Systems for Producing Antibody from MammalianCells . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 DarrinKuystermansandMohamedAl-Rubeai 3 ProductionofAntibodyinInsectCells . . . . . . . . . . . . . . . . 53 HidekiYamaji 4 ProductionofMonoclonalAntibodiesinGlycoengineered Pichiapastoris . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77 DongxingZha,ThomasLinden,andThomasPotgieter 5 ProductionofAntibodiesinHansenulapolymorpha . . . . . . . . . 99 GeorgMelmer,StephanHellwig,GritHehmann,andUlrikeDahlems 6 ProductionofAntibodybyTransgenicAvians . . . . . . . . . . . . 121 YoshinoriKawabeandMasamichiKamihira 7 ProductionofAntibodiesinPlants . . . . . . . . . . . . . . . . . . 143 AlejandroSarrion-Perdigones,PalomaJuarez, AntonioGranell,andDiegoOrzaez 8 Production of Antibody Fab Fragments inEscherichiacoli . . . . . . . . . . . . . . . . . . . . . . . . . . . 165 HiroshiTachibanaandMasatakaTakekoshi 9 IntrabodyExpressioninMammalianCells. . . . . . . . . . . . . . 179 SilviaBiocca 10 EngineeringAntibodiesforCancerTherapy . . . . . . . . . . . . . 197 LisaE.GoldsmithandMatthewK.Robinson 11 Recombinant Bispecific Antibodies forCancerTherapy . . . . . . . . . . . . . . . . . . . . . . . . . . 235 DafneMüllerandRolandE.Kontermann v vi Contents 12 TheRoleofGlycosylationinTherapeuticAntibodies . . . . . . . . 251 MaureenSpearman,BenDionne,andMichaelButler 13 QualityIssuesArisingfromPost-translationalModification ofRecombinantAntibodies . . . . . . . . . . . . . . . . . . . . . . 293 RaymondTytherandNigelJenkins 14 RecoveryandPurificationofAntibody . . . . . . . . . . . . . . . . 305 XueJunHan,ArthurHewig,andGaneshVedantham SubjectIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 341 Contributors MohamedAl-Rubeai SchoolofChemicalandBioprocessEngineering,and ConwayInstituteofBiomolecularandBiomedicalResearch,UniversityCollege Dublin,Dublin,Ireland,[email protected] SilviaBiocca DepartmentofNeuroscience,UniversityofRome“TorVergata”, 00133Rome,Italy,[email protected] MichaelButler DepartmentofMicrobiology,UniversityofManitoba,Winnipeg, MB,CanadaR3T2N2,[email protected] UlrikeDahlems PharmedArtisGmbH,Forckenbeckstr.6,D-52074Aachen, Germany,[email protected] BenDionne DepartmentofMicrobiology,UniversityofManitoba,Winnipeg, MB,CanadaR3T2N2,[email protected] LisaE.Goldsmith DevelopmentalTherapeuticsProgram,FoxChaseCancer Center,Philadelphia,PA19111,USA,[email protected] AntonioGranell InstitutodeBiologíaMolecularyCelulardePlantas(IBMCP), UPV-CSIC,46022Valencia,Spain,[email protected] XueJunHan AmgenInc.,Seattle,WA98119,USA,[email protected] GritHehmann PharmedArtisGmbH,Forckenbeckstr.6,D-52074Aachen, Germany,[email protected] StephanHellwig PharmedArtisGmbH,Forckenbeckstr.6,D-52074Aachen, Germany,[email protected] ArthurHewig AmgenInc.,Seattle,WA98119,USA,[email protected] NigelJenkins NationalInstituteforBioprocessingReasearchandTraining, UniversityCollegeDublin,Dublin,Ireland,[email protected] ThomasJostock NovartisPharmaAG,CH-4002Basel,Switzerland, [email protected] PalomaJuarez InstitutodeBiologíaMolecularyCelulardePlantas(IBMCP), UPV-CSIC,46022Valencia,Spain,[email protected] vii viii Contributors MasamichiKamihira DepartmentofChemicalEngineering,Facultyof Engineering,KyushuUniversity,Fukuoka819-0395,Japan, [email protected] YoshinoriKawabe DepartmentofChemicalEngineering,Facultyof Engineering,KyushuUniversity,Fukuoka819-0395,Japan, [email protected] RolandE.Kontermann InstitutfürZellbiologieundImmunologie,Universität Stuttgart,70569Stuttgart,Germany,[email protected] DarrinKuystermans SchoolofChemicalandBioprocessEngineering,and ConwayInstituteofBiomolecularandBiomedicalResearch,UniversityCollege Dublin,Dublin,Ireland,[email protected] ThomasLinden BioprocessDevelopment,Merck&Co,Rahway,NJ07065, USA,[email protected] GeorgMelmer PharmedArtisGmbH,Forckenbeckstr.6,D-52074Aachen, Germany,[email protected] DafneMüller InstitutfürZellbiologieundImmunologie,UniversitätStuttgart, 70569Stuttgart,Germany,[email protected] DiegoOrzaez InstitutodeBiologíaMolecularyCelulardePlantas(IBMCP), UPV-CSIC,46022Valencia,Spain,[email protected] ThomasPotgieter BioprocessDevelopment,Merck&Co,Rahway,NJ07065, USA,[email protected] MatthewK.Robinson DevelopmentalTherapeuticsProgram,FoxChaseCancer Center,Philadelphia,PA19111,USA,[email protected] AlejandroSarrion-Perdigones InstitutodeBiologíaMolecularyCelularde Plantas(IBMCP),UPV-CSIC,46022Valencia,Spain,[email protected] MaureenSpearman DepartmentofMicrobiology,UniversityofManitoba, Winnipeg,MB,CanadaR3T2N2,[email protected] HiroshiTachibana DepartmentofInfectiousDiseases,TokaiUniversitySchool ofMedicine,Isehara,Kanagawa259-1193,Japan,[email protected] MasatakaTakekoshi DepartmentofMolecularLifeScience,TokaiUniversity SchoolofMedicine,Isehara,Kanagawa259-1193,Japan, [email protected] RaymondTyther NationalInstituteforBioprocessingReasearchandTraining, UniversityCollegeDublin,Dublin,Ireland,[email protected] GaneshVedantham AmgenInc.,Seattle,WA98119,USA, [email protected] Contributors ix HidekiYamaji DepartmentofChemicalScienceandEngineering,Graduate SchoolofEngineering,KobeUniversity,Kobe657-8501,Japan, [email protected] DongxingZha GlycoFiInc.(Awholly-ownedsubsidiaryofMerck&CoInc.), Lebanon,NH03766,USA,[email protected]
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