Andrology Laboratory Manual

245 Pages·2010·8.6 MB·English

by  Kamini A. Rao

Andrology
Laboratory Manual

Andrology
Laboratory Manual
Editors
Kamini A Rao DGO DORCP DCh FRCOG (UK), MCh (UK), FICOG, PGDMLE (Law)
Medical Director, Bangalore Assisted Conception Centre
6/7 Kumara Krupa Road High Grounds
Bengaluru, Karnataka, India
Ashok Agarwal PhD, HCLD (ABB)
Director, Andrology Laboratory and Reproductive Tissue Bank
Director, Center for Reproductive Medicine and ART Training
Professor, Cleveland Clinic Lerner College of Medicine
Case Western Reserve University, Staff, Glickman Urological and Kidney Institute, Ob-Gyn and Wenen’s Health
Institute, Anatomic Pathology and Immunology, Cleveland Clinic 9500 Euclid Avenue Desk A 19.1 Cleveland,
Ohio 44195, USA
MS Srinivas PhD
Scientific Director and Chief Embryologist
Bangalore Assisted Conception Centre, Bengaluru, Karnataka, India
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Andrology Laboratory Manual
© 2010, Jaypee Brothers Medical Publishers (P) Ltd
All rights reserved. No part of this publication should be reproduced, stored in a retrieval system, or transmitted in any form or by
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any dispute, all legal matters are to be settled under Delhi jurisdiction only.
First Edition: 2010
ISBN 978-81-8448-901-9
Typeset at JPBMP typesetting unit
Printed at Ajanta Offset
CONTRIBUTORS
Ahmed M Ragheb MD, M Sc Ashok Bhattacharyya
Department of Biochemistry, University of Calcutta, Kolkata,
Fellow in Male Infertility
West Bengal, India
Glickman Urological and Kidney Institute, Cleveland Clinic
9500 Euclid Avenue, Cleveland, OH 44195, USA
Baidyanath Chakravarty
Alberto M Torres-Cantero MD, MPH, PH Director
Institute of Reproductive Medicine, Kolkata
Professor
West Bengal, India
Division of Preventive Medicine and Public Health, School of
Medicine, University of Murcia, Campus de Espinardo, Murcia,
Baiju PA
Spain
Consultant
Bangalore Assisted Conception Centre, 6/7, Kumara Krupa
Alex C Varghese
Road, High Grounds, Bengaluru, Karnataka, India
Scientific Director and Chief
Embryologist Fertility Clinic and IVF Division AMRI Medical Daniel R Franken
Centre (A Unit of AMRI Hospitals) 97A, Southern Avenue, Reproductive Biology Research Laboratory, Department of
Kolkata, West Bengal, India Obstetrics and Gynaecology, Tygerberg 7505, South Africa
Andrew Luu Edmund Sabanegh Jr MD
Center for Reproductive Medicine Director
Cleveland Clinic 9500 Euclid Avenue, Desk A19.1 Cleveland, Ohio Center for Male Fertility Glickman Urological and Kidney
44195 USA Institute Cleveland Clinic 9500 Euclid Avenue/Q100
Cleveland, OH United States
Ang Wen Jeat
Melaka Manipal Medical College, Eleonora Bedin Pasqualotto MD, PhD
Jalan Batu Hampar, Bukit Baru 75150, Melaka, Malaysia Professor of Gynecology
University of Caxias do Sul, RS, Brazil, Chief Obstetrics and
Anjali Chandra Gynecology department, General Hospital, University of Caxias
Research Fellow Cleveland Clinic 9500 Euclid Avenue, Desk do Sul, RS, Brazil Researcher II, Brazilian Counsel of Scientific
A19.1 Cleveland, Ohio, 44195 USA and Technological Develop-ment (CNPq) Director Conception –
Center for Human Reproduction Rua Pinheiro Machado, 2569,
Antonio Tavares MD Sl 23/24, Bairro Sao Pelegrino, Caxias do Sul, RS, Brazil
Director
Fabio Firmbach Pasqualotto MD, PhD
Rubinstein Urological Clinic Rubinstein Urological Clinic
Professor of Urology
Estrada Pau Ferro 204 AP 101 BL 1 Jacarepagua, Rio De Janeiro,
University of Caxias do Sul, RS, Brazil, Professor of
Brazil
postgraudation, Institute of Biotechnology, University of
Ashok Agarwal PhD HCLD (ABB) Caxias do Sul, RS, Brazil, Researcher I, Brazilian Counsel of
Scientific and Technological Development (CNPq) Director
Director
Conception – Center for Human Reproduction, Rua Pinheiro
Andrology Laboratory and Reproductive Tissue Bank, Director, Machado, 2569, Sl 23/24, Bairro Sao Pelegrino, Caxias do Sul,
Center for Reproductive Medicine and ART Training, Professor, RS Brazil
Cleveland Clinic Lerner College of Medicine, Case Western
Reserve University Staff, Glickman Urological and Kidney Fnu Deepinder
Institute, Ob-Gyn and Women’s Health Institute, Anatomic Department of Internal Medicine
Pathology and Immunology Cleveland Clinic 9500 Euclid St Joseph’s Hospital, Resurrection Health Care System,
Avenue, Desk A 19.1 Cleveland, Ohio, 44195 USA University of Illinois, USA
vi ANDROLOGY LABORATORY MANUAL
Furquan Pathan MS Srinivas PhD
Melaka Manipal Medical College, Scientific Director and Chief
Jalan Batu Hampar, Bukit Baru 75150, Melaka, Malaysia Embryologist, Bangalore Assisted Conception Centre,
Bengaluru, Karnataka, India
Hans-Juergen Glander
Natachandra Chimote
Department of Dermatology/Andrology Unit, European
Academy of Andrology (EAA) Training Center, University of Scientific Director
Leipzig, Ph.-Rosenthal-Str 23-25, Leipzig 04103, Germany Vasundara Clinic and Assisted Conception Centre, Nagpur,
India
Jaime Mendiola MSc PhD
Parag Nandi
Research Fellow
Research Fellow
Division of Preventive Medicine and Public Health, School of
Department of Environmental Sciences, University of Calcutta,
Medicine, University of Murcia, Campus de Espinardo, Murcia,
Kolkata, West Bengal, India
Spain
Philip Kumanov
James Savia
Clinical Center of Endocrinology and Gerontology Medical
Research Fellow
University Sofia 6 Damian Gruev Str. 1303 Sofia, Bulgaria
Cleveland Clinic 9500 Euclid Avenue-Q100 Cleveland, OH,
United States Rakesh Sharma
Center for Reproductive Medicine
John C Kefer MD, PhD
Cleveland Clinic 9500 Euclid Avenue, Desk A19.1 Cleveland,
Clinical Fellow, Center for Male Fertility Glickman Urological Ohio 44195 USA
and Kidney Institute Cleveland Clinic 9500 Euclid Avenue/
Q100, Cleve land, OH United States Reda Mahfouz
Center for Reproductive Medicine
Kamini A Rao
Cleveland Clinic 9500 Euclid Avenue, Desk A19.1 Cleveland,
Medical Director Ohio 44195 USA
Bangalore Assisted Conception Centre, 6/7, Kumara Krupa
Road, High Grounds, Bengaluru, Karnataka, India Rima Dada MD, PhD (Genetics)
Associate Professor
Kelly S Athayde MT, TS (ABB) MS
All India Institute of Medical Sciences, Ansari Nagar, New
Embryologist Incharge, Fertility Unlimited, Inc., Adjunct Delhi, India
Instructor Ob-Gyn, Northeastern Ohio Univer-sities Colleges
of Medicine and Pharmacy Neoucom, 468 E Market Street Rupin Shah
Akron, Ohio, USA Consultant
Andrology and Microsurgeon, Lilavathi Hospital and Research
Kerstin Poenicke
Centre, Mumbai Maharashtra, India
Department of Andrology, University of Leipzig, Leipzig,
Sathish Adiga
Germany
Associate Professor
Luana Venturin Lara Department of Obstetrics and Gynecology Division of
Biologist, Conception – Center for Human Reproduction, Rua Reproductive Medicine Department of Obstetrics and Gynecology
Pinheiro Machado, 2569, Sl 23/24, Bairro Sao Pelegrino, Caxias Kasturba Medical College, Manipal, India
do Sul, RS Brazil
Shukla Kundu
Meena Chimote Research fellow
Department of Biochemistry, University of Calcutta, Kolkata,
Medical Director
West Bengal, India
Vasundara Clinic and Assisted Conception Centre, Nagpur,
India
Sonja Grunewald
Monis Bilal Shamsi MSc Physician
Sundararajan Venkatesh M Pharm Lab for Molecular University of Leipzig Department of Dermatology, Venerology
Reproduction and Genetics, Anatomy Department, AIIMS, New and Allergology European Training Center of Andrology
Delhi, India Philipp-Rosenthal-Strasse 23-25 04103 Leipzig, Germany
CONTRIBUTORS vii
Sundararajan Venkatesh Vasan SS
Director
Lab for Molecular Reproduction and Genetics
Manipal Andrology and Reproductive Services (MARS)
Anatomy Department, AIIMS
Director
New Delhi, India
Ankur – (Centre for Men’s Health, Fertility and Reproductive
Health, Neurourology and Continence)
Uwe Paasch
Head
Andrologist (EAA) Senior Physician NU Hospitals Centre for Male Reproduction and Erectile
University of Leipzig dysfunction
Department of Dermatology, Venerology and Allergology, Vijay Mangoli
European Training Center of Andrology Fertility Clinic and IVF Centre, Kwality House, Mumbai,
Division of Dermato-pathology Maharashtra, India
Division of Aesthetics and Laserdermatology
Vineet Malhotra
Philipp-Rosenthal-Strasse 23-2504103
Assistant Urologist and Research Fellow Lilavathi Hospital and
Leipzig, Germany Research Centre, Mumbai Maharashtra, India

FOREWORD
It gives me great pleasure to write the foreword to this book Andrology Laboratory Manual.
Its aim is to provide detailed information for the interdisciplinary team of specialists from
the clinical members: gynecologists, endocrinologists and urologists to the clinical scientists
and embryologists. It encompasses both the latest laboratory techniques and clinical
treatments. Our fundamental knowledge is rejuvenated with a concise overview of
spermatogenesis, sperm maturation and the genetics of male infertility. Our understanding
of the complexity and vulnerability of these processes is enhanced by up-to-date information
of the latest advances in male infertility evaluation. Chapters for clinicians include novel
information on endocrine disorders, compromising lifestyle factors, sexual dysfunction and
surgical sperm retrieval techniques. Chapters for scientists focus on laboratory skills in sperm preparation, protection
from infection and cryopreservation. Its format makes it useful as an introductory text and also as an everyday
reference resource for those working in the field of Clinical Andrology. I recommend it as a useful, accessible book
for every fertility center.
Sheena EM Lewis
Professor of Reproductive Medicine
Centre for Public Health
Queen’s University of Belfast
Institute of Clinical Science
Northern Ireland, UK