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AN INTRODUCTION TO STATISTICAL ANALYSIS IN RESEARCH PDF

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AnIntroductiontoStatistical AnalysisinResearch An Introduction to Statistical Analysis in Research WithApplicationsintheBiologicalandLifeSciences KathleenF.Weaver VanessaC.Morales SarahL.Dunn KanyaGodde PabloF.Weaver Thiseditionfirstpublished2018 ©2018JohnWiley&Sons,Inc Allrightsreserved.Nopartofthispublicationmaybereproduced,storedinaretrievalsystem,or transmitted,inanyformorbyanymeans,electronic,mechanical,photocopying,recordingorotherwise, exceptaspermittedbylaw.Adviceonhowtoobtainpermissiontoreusematerialfromthistitleisavailable athttp://www.wiley.com/go/permissions. TherightofKathleenF.Weaver,VanessaC.Morales,SarahL.Dunn,KanyaGodde,andPabloF.Weaverto beidentifiedastheauthorsofthisworkhasbeenassertedinaccordancewithlaw. RegisteredOffices JohnWiley&Sons,Inc.,111RiverStreet,Hoboken,NJ07030,USA EditorialOffice 111RiverStreet,Hoboken,NJ07030,USA Fordetailsofourglobaleditorialoffices,customerservices,andmoreinformationaboutWileyproducts visitusatwww.wiley.com. Wileyalsopublishesitsbooksinavarietyofelectronicformatsandbyprint-on-demand.Somecontentthat appearsinstandardprintversionsofthisbookmaynotbeavailableinotherformats. LimitofLiability/DisclaimerofWarranty Thepublisherandtheauthorsmakenorepresentationsorwarrantieswithrespecttotheaccuracyor completenessofthecontentsofthisworkandspecificallydisclaimallwarranties,includingwithout limitationanyimpliedwarrantiesoffitnessforaparticularpurpose.Thisworkissoldwiththe understandingthatthepublisherisnotengagedinrenderingprofessionalservices.Theadviceand strategiescontainedhereinmaynotbesuitableforeverysituation.Inviewofongoingresearch,equipment modifications,changesingovernmentalregulations,andtheconstantflowofinformationrelatingtothe useofexperimentalreagents,equipment,anddevices,thereaderisurgedtoreviewandevaluatethe informationprovidedinthepackageinsertorinstructionsforeachchemical,pieceofequipment,reagent, ordevicefor,amongotherthings,anychangesintheinstructionsorindicationofusageandforadded warningsandprecautions.Thefactthatanorganizationorwebsiteisreferredtointhisworkasacitation and/orpotentialsourceoffurtherinformationdoesnotmeanthattheauthororthepublisherendorsesthe informationtheorganizationorwebsitemayprovideorrecommendationsitmaymake.Further,readers shouldbeawarethatwebsiteslistedinthisworkmayhavechangedordisappearedbetweenwhenthis workswaswrittenandwhenitisread.Nowarrantymaybecreatedorextendedbyanypromotional statementsforthiswork.Neitherthepublishernortheauthorshallbeliableforanydamagesarising herefrom. LibraryofCongressCataloging-in-PublicationData Names:Weaver,KathleenF. Title:Anintroductiontostatisticalanalysisinresearch:with applicationsinthebiologicalandlifesciences/KathleenF.Weaver[andfourothers]. Description:Hoboken,NJ:JohnWiley&Sons,Inc.,2017.|Includesindex. Identifiers:LCCN2016042830|ISBN9781119299684(cloth)|ISBN9781119301103(epub) Subjects:LCSH:Mathematicalstatistics–Dataprocessing.|Multivariate analysis–Dataprocessing.|Lifesciences–Statisticalmethods. Classification:LCCQA276.4.I652017|DDC519.5–dc23LCrecordavailable athttps://lccn.loc.gov/2016042830 Coverimage:Courtesyoftheauthor CoverdesignbyWiley Setin11/14ptWarnockProbyAptaraInc.,NewDelhi,India 10 9 8 7 6 5 4 3 2 1 v Contents Preface ix Acknowledgments xi AbouttheCompanionWebsite xiii  ExperimentalDesign 1 1.1 ExperimentalDesignBackground 1 1.2 SamplingDesign 2 1.3 SampleAnalysis 7 1.4 Hypotheses 9 1.5 Variables 10  CentralTendencyandDistribution 13 2.1 CentralTendencyandOtherDescriptiveStatistics 13 2.2 Distribution 18 2.3 DescriptiveStatisticsinExcel 34 2.4 DescriptiveStatisticsinSPSS 48 2.5 DescriptiveStatisticsinNumbers 52 2.6 DescriptiveStatisticsinR 57  ShowingYourData 61 3.1 BackgroundonTablesandGraphs 61 3.2 Tables 62 3.3 BarGraphs,Histograms,andBoxPlots 63 3.4 LineGraphsandScatterPlots 136 3.5 PieCharts 165  ParametricversusNonparametricTests 191 4.1 Overview 192 4.2 Two-SampleandThree-SampleTests 194  t-Test 195 5.1 Student’st-TestBackground 195 vi Contents 5.2 Examplest-Tests 196 5.3 CaseStudy 201 5.4 ExcelTutorial 205 5.5 Pairedt-TestSPSSTutorial 209 5.6 Independentt-TestSPSSTutorial 213 5.7 NumbersTutorial 218 5.8 RIndependent/Paired-Samplest-TestTutorial 223  ANOVA 227 6.1 ANOVABackground 227 6.2 CaseStudy 236 6.3 One-WayANOVAExcelTutorial 241 6.4 One-WayANOVASPSSTutorial 247 6.5 One-WayRepeatedMeasuresANOVASPSSTutorial 252 6.6 Two-WayRepeatedMeasuresANOVASPSSTutorial 261 6.7 One-WayANOVANumbersTutorial 272 6.8 One-WayRTutorial 288 6.9 Two-WayANOVARTutorial 291  Mann–WhitneyUandWilcoxonSigned-Rank 297 7.1 Mann–WhitneyUandWilcoxonSigned-RankBackground 297 7.2 Assumptions 298 7.3 CaseStudy–Mann—WhitneyUTest 299 7.4 CaseStudy–WilcoxonSigned-Rank 302 7.5 Mann–WhitneyUExcelTutorial 305 7.6 WilcoxonSigned-RankExcelTutorial 313 7.7 Mann–WhitneyUSPSSTutorial 319 7.8 WilcoxonSigned-RankSPSSTutorial 324 7.9 Mann–WhitneyUNumbersTutorial 328 7.10 WilcoxonSigned-RankNumbersTutorial 337 7.11 Mann–WhitneyU/WilcoxonSigned-RankRTutorial 350  Kruskal–Wallis 353 8.1 Kruskal–WallisBackground 353 8.2 CaseStudy1 354 8.3 CaseStudy2 358 8.4 Kruskal–WallisExcelTutorial 362 8.5 Kruskal–WallisSPSSTutorial 368 8.6 Kruskal–WallisNumbersTutorial 375 8.7 Kruskal–WallisRTutorial 386  Chi-SquareTest 393 9.1 Chi-SquareBackground 393 9.2 CaseStudy1 394 Contents vii 9.3 CaseStudy2 401 9.4 Chi-SquareExcelTutorial 405 9.5 Chi-SquareSPSSTutorial 418 9.6 Chi-SquareNumbersTutorial 426 9.7 Chi-SquareRTutorial 429  Pearson’sandSpearman’sCorrelation 435 10.1 CorrelationBackground 435 10.2 Example 435 10.3 CaseStudy–Pearson’sCorrelation 442 10.4 CaseStudy–Spearman’sCorrelation 445 10.5 Pearson’sCorrelationExcelandNumbersTutorial 448 10.6 Spearman’sCorrelationExcelTutorial 455 10.7 Pearson/Spearman’sCorrelationSPSSTutorial 462 10.8 Pearson/Spearman’sCorrelationRTutorial 467  LinearRegression 473 11.1 LinearRegressionBackground 473 11.2 CaseStudy 480 11.3 LinearRegressionExcelTutorial 484 11.4 LinearRegressionSPSSTutorial 497 11.5 LinearRegressionNumbersTutorial 508 11.6 LinearRegressionRTutorial 517  BasicsinExcel 523 12.1 OpeningExcel 524 12.2 InstallingtheDataAnalysisToolPak 525 12.3 CellsandReferencing 529 12.4 CommonCommandsandFormulas 532 12.5 ApplyingCommandstoEntireColumns 534 12.6 InsertingaFunction 536 12.7 FormattingCells 537  BasicsinSPSS 539 13.1 OpeningSPSS 539 13.2 LabelingVariables 541 13.3 SettingDecimalPlacement 543 13.4 DeterminingtheMeasureofaVariable 544 13.5 SavingSPSSDataFiles 545 13.6 SavingSPSSOutput 547  BasicsinNumbers 551 14.1 OpeningNumbers 551 14.2 CommonCommands 553 viii Contents 14.3 ApplyingCommands 555 14.4 AddingFunctions 557  BasicsinR 561 15.1 OpeningR 561 15.2 GettingAcquaintedwiththeConsole 562 15.3 LoadingData 566 15.4 InstallingandLoadingPackages 570 15.5 Troubleshooting 576  Appendix 579 FlowChart 579 LiteratureCited 581 Glossary 585 Index 591 ix Preface This book is designed to be a practical guide to the basics of statis- tical analysis. The structure of the book was born from a desire to meet the needs of our own science students, who often felt discon- nected from the mathematical basis of statistics and who struggled with the practical application of statistical analysis software in their research.Thus,thespecificemphasisofthistextisontheconceptual framework of statistics and the practical application of statistics in thebiologicalandlifesciences,withexamplesandcasestudiesfrom biology,kinesiology,andphysicalanthropology. Inthefirstfewchapters,thebookfocusesonexperimentaldesign, showing data, and the basics of sampling and populations. Under- standing biases and knowing how to categorize data, process data, and show data in a systematic way are important skills for any researcher. By solidifying the conceptual framework of hypothesis testingandresearchmethods,aswellasthepracticalinstructionsfor showing data through graphs and figures, the student will be better equippedforthestatisticalteststocome. Subsequent chapters delve into detail to describe many of the parametric and nonparametric statistical tests commonly used in research. Each section includes a description of the test, as well as when and how to use the test appropriately in the context of exam- plesfrombiologyandthelifesciences.Thechaptersincludein-depth tutorials for statistical analyses using Microsoft Excel, SPSS, Apple Numbers, and R, which are the programs used most often on col- legecampuses,orinthecaseofR,isfreetoaccessontheweb.Each tutorialincludessampledatasetsthatallowforpracticingandapply- ingthestatisticaltests,aswellasinstructionsonhowtointerpretthe x Preface statistical outputs in the context of hypothesis testing. By building confidencethroughpracticeandapplication,thestudentshouldgain the proficiency needed to apply the concepts and statistical tests to theirownsituations. The material presented within is appropriate for anyone looking toapplystatisticalteststodata,whetheritisforthenovicestudent, forthestudentlookingtorefreshtheirknowledgeofstatistics,orfor those looking for a practical step-by-step guide for analyzing data acrossmultipleplatforms.Thisbookisdesignedforundergraduate- level research methods and biostatistics courses and would also be usefulasanaccompanyingtexttoanystatisticscourseorcoursethat requiresstatisticaltestinginitscurriculum. ExamplesfromtheBook Thetutorialsinthisbookarebuilttoshowavarietyofapproachesto using Microsoft Excel, SPSS, Apple Numbers, and R, so the student can find their own unique style in working with statistical software, aswellastoenrichthestudentlearningexperiencethroughexposure tomoreandvariedexamples.Mostofthedatausedinthisbookwere obtaineddirectlyfrompublishedarticlesorweredrawnfromunpub- lisheddatasetswithpermissionfromthefacultyattheUniversityof LaVerne.Insometutorials,dataweregeneratedstrictlyforteaching purposes;however,datawerebasedonactualtrendsobservedinthe literature. xi Acknowledgments Thisbookwasmadepossiblebythehelpandsupportofmanyclose colleagues, students, friends, and family; because of you, the ideas forthisbookbecameareality.ThankyoutoJeromeGarciaandAnil Kapoorforincorporatingearlydraftsofthisbookintoyourcourses and for your constructive feedback that allowed it to grow and develop. Thank you to Priscilla Escalante for your help in research- ingtutorialdesign,AliciaGuadarramaandJeremyWagonerforbeing ourtutorialtesters,andMargaretGoughandJosephCabreraforyour helpful comments and suggestions; we greatly appreciate it. Finally, thankyoutotheUniversityofLaVernefacultythatkindlyprovided their original data to be used as examples and to the students who inspiredthisworkfromthebeginning.

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