Adult Stem Cells: Methods and Protocols

320 Pages·2017·10.328 MB·English

by  Paolo Di Nardo, Sanjiv Dhingra, Dinender K. Singla (eds.)

Methods in
Molecular Biology 1553
Paolo Di Nardo · Sanjiv Dhingra
Dinender K. Singla Editors
Adult
Stem Cells
Methods and Protocols
M M B
ethods in olecular iology
Series Editor
John M. Walker
School of Life and Medical Sciences
University of Hertfordshire
Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes:
http://www.springer.com/series/7651
Adult Stem Cells
Methods and Protocols
Edited by
Paolo Di Nardo
Center for Regenerative Medicine, Università di Roma Tor Vergata, Rome, Italy; Department of Clinical
Sciences and Translational Medicine, Università di Roma Tor Vergata, Rome, Italy
Sanjiv Dhingra
Institute of Cardiovascular Sciences, St-Boniface Hospital Albrechtsen Research Centre, Regenerative
Medicine Program, College of Medicine, Faculty of Health Sciences, University of Manitoba,
Winnipeg, MB, Canada
Dinender K. Singla
Division of Metabolic and Cardiovascular Sciences, Burnett School of Biomedical Sciences,
University of Central Florida, Orlando, FL, USA
Editors
Paolo Di Nardo Sanjiv Dhingra
Center for Regenerative Medicine Institute of Cardiovascular Sciences
Università di Roma Tor Vergata St-Boniface Hospital Albrechtsen Research Centre
Rome, Italy Regenerative Medicine Program
College of Medicine
Department of Clinical Sciences and Translational
Faculty of Health Sciences
Medicine
University of Manitoba
Università di Roma Tor Vergata
Winnipeg, MB, Canada
Rome, Italy
Dinender K. Singla
Division of Metabolic and Cardiovascular Sciences
Burnett School of Biomedical Sciences
University of Central Florida
Orlando, FL, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic)
Methods in Molecular Biology
ISBN 978-1-4939-6754-4 ISBN 978-1-4939-6756-8 (eBook)
DOI 10.1007/978-1-4939-6756-8
Library of Congress Control Number: 2016963441
© Springer Science+Business Media LLC 2017
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Cover Caption: Immunofluoresce micrograph of alpha-sarcomeric actin (green) in differentiating cardiac progenitor cells
grown on a biodegradable scaffold (phase contrast microscopy, Blue). Nuclei were stained with propidium iodide (red)
(Felicia Carotenuto and Paolo Di Nardo)
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Preface
Degenerative diseases are the main cause of death globally and, owing to the lack of innova-
tive drugs and several limits of organ transplantation, new cost-effective solutions for treat-
ing damaged organs are compelling needs. In this respect, the prowess of stem cells to repair
tissues damaged by degenerative disorders has created universal interest, even if the related
technologies are still in their infancy. Indeed, decades of intensive studies on stem cell behav-
ior have not generated yet the clinical revolution so much announced and expected. This
situation has been determined by many impeding factors, among which the lack of standard-
ized protocols to isolate and handle stem cells has played a major role. Stem cell technology
is among the most complex research endeavors and requires strict standardization of the
materials and procedures, through a long-term process driven by merging the quantum of
knowledge resident in different disciplines and international laboratories. This implies a
strong impetus for change, in which cross-fertilization is encouraged to create a positive and
explosive melting pot of ideas, methods, and expertise to benefit research and sustain
advanced biomedical industries. Therefore, we should be aware that Stem Cell Standardization
cannot be a concern of the biomedical field alone, but must involve the knowledge accumu-
lated in a multiplicity of fields. The convergence between biomedicine and engineering holds
promise to benefit both the patients and the industries. In this way only, we can hope to
transform an artisanal activity, as stem cell research is today, in an industrialized process able
to supply patients with efficient, safe, and cost-effective cell treatments.
This book collects stem cell protocols from some of the major laboratories involved in
stem cell research in the world. They have made available their protocols in the hope to
ignite a fruitful discussion on adult stem cells standardization to take further the first enthu-
siastic wave that has often produced only fragmented knowledge and unsuitable options for
innovative treatments to be delivered in the clinical setting.
Inevitably, the book does not report protocols related to the isolation and culture of
stem cells in all possible living tissues. The editors have selected some of those that they
considered most reliable; it does not mean that the protocols excluded should be consid-
ered of minor interest.
Finally, the editors wish to express their deepest gratitude to Anna Maria Maccari for
the invaluable support in collecting and helping to revise all manuscripts.
Rome, Italy Paolo Di Nardo
Winnipeg, MB, Canada Sanjiv Dhingra
Orlando, FL, USA Dinender Singla
v
Contents
Preface.......................................................... v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Simple Protocol to Isolate, Characterize, and Expand Dental
Pulp Stem Cells................................................ 1
Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere,
Zhiqian Yang, and Giovanni Nicolao Berta
2 Derivation of Mesenchymal Stem Cells from Embryonic Stem Cells:
A Non-Variable and Inexhaustive Source of Adult Stem Cells ............. 15
Glen Lester Sequiera, Niketa Sareen, Ejlal Abu El-Rub,
and Sanjiv Dhingra
3 Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs)
for the Treatment of Sjogren’s-Like Disease in NOD Mice ............... 25
Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang,
and Simon D. Tran
4 Manipulating the Proliferative Potential of Cardiomyocytes
by Gene Transfer............................................... 41
Giulia Prosdocimo and Mauro Giacca
5 Assessment of Energy Metabolic Changes in Adipose Tissue-Derived
Stem Cells.................................................... 55
Ghazaleh Hajmousa and Martin C. Harmsen
6 Isolation and In Vitro Characterization of Epidermal Stem Cells........... 67
Kasper S. Moestrup, Marianne S. Andersen, and Kim B. Jensen
7 Endothelial Progenitor Cells: Procedure for Cell Isolation and Applications ..... 85
Garikipati V.N. Srikanth and Raj Kishore
8 Therapeutic Application of Placental Mesenchymal Stem Cells
Reprogrammed Neurospheres in Spinal Cord Injury of SCID............. 91
Vikram Sabapathy, Franklin Jebaraj Herbert, and Sanjay Kumar
9 Therapeutic Application of Human Wharton Jelly Mesenchymal
Stem Cells in Skin Injury of SCID.................................. 115
Vikram Sabapathy, Balasubramanian Sundaram, and Sanjay Kumar
10 Isolation, Characterization, and Expansion of Cancer Stem Cells........... 133
Luke A. Torre-Healy, Artem Berezovsky, and Justin D. Lathia
11 Cardiac Progenitor Cell Extraction from Human Auricles................ 145
Paolo Di Nardo and Francesca Pagliari
12 Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle ........ 155
Antonio Musarò and Silvia Carosio
13 Isolation of Stromal Stem Cells from Adipose Tissue.................... 169
Maria Prat, Francesca Oltolina, Silvia Antonini,
and Andrea Zamperone
vii
viii Contents
14 Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells
as an Individual-Specific and Renewable Source of Adult Stem Cells ........ 183
Glen Lester Sequiera, Sekaran Saravanan, and Sanjiv Dhingra
15 A Simplified and Systematic Method to Isolate, Culture, and Characterize
Multiple Types of Human Dental Stem Cells from a Single Tooth.......... 191
Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen,
Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki,
Nicholas Makhoul, Huan Pham, Yoshinori Sumita, and Simon D. Tran
16 Measurement of Autophagy by Flow Cytometry . . . . . . . . . . . . . . . . . . . . . . . 209
Silvia Zappavigna, Angela Lombardi, Gabriella Misso, Anna Grimaldi,
and Michele Caraglia
17 Echocardiography-Guided Intramyocardial Injection Method
in a Murine Model ............................................. 217
Kay Maeda, Rick Seymour, Marc Ruel, and Erik J. Suuronen
18 Noninvasive Assessment of Cell Fate and Biology in Transplanted
Mesenchymal Stem Cells......................................... 227
Federico Franchi and Martin Rodriguez-Porcel
19 Methods for Long-Term Storage of Murine Bone Marrow- Derived
Mesenchymal Stem Cells......................................... 241
Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil,
and Sanjiv Dhingra
20 Therapeutic Application of Adult Stem Cells in the Heart ................ 249
Taylor A. Johnson and Dinender K. Singla
21 Patient-Derived and Intraoperatively Formed Biomaterial
for Tissue Engineering .......................................... 265
Shalmli U. Joshi, Rares O. Barbu, Melissa Carr-Reynolds, Brian Barnes,
and Syam P. Nukavarapu
22 Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal
Stromal Cells for Rabbit Mandibular Reconstruction and Engineering....... 273
Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah,
Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi,
and Simon D. Tran
23 Optimal Environmental Stiffness for Stem Cell Mediated Ischemic
Myocardium Repair ............................................ 293
Honghai Liu, Christian Paul, and Meifeng Xu
24 Assessment of MiRNA Regulation of Endothelial Progenitor Cell Mediated
Angiogenesis.................................................. 305
Darukeshwara Joladarashi and Prasanna Krishnamurthy
25 Stem Cell Exosomes: Cell-FreeTherapy for Organ Repair ................ 315
Mohsin Khan and Raj Kishore
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 323
Contributors
MohaMed Nur abdallah • Faculty of Dentistry, McGill University, Montreal, QC,
Canada
ejlal abu el-rub • Institute of Cardiovascular Sciences, St-Boniface Hospital Albrechtsen
Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health
Sciences, University of Manitoba, Winnipeg, MB, Canada
MariaNNe S. aNderSeN • Biotech Research and Innovation Centre, University of
Copenhagen, København, Denmark
Silvia aNtoNiNi • Department of Health Sciences, Università del Piemonte Orientale
“A .Avogadro”, Novara, Italy
MohaMMed bakkar • Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty
of Dentistry, McGill University, Montreal, QC, Canada
rareS o. barbu • Department of Biomedical Engineering, University of Connecticut,
Storrs, CT, USA
briaN barNeS • Arteriocyte, Cellular Therapies Medical Systems, Hopkinton, MA, USA
arteM berezovSky • Department of Cellular and Molecular Medicine, Lerner Research
Institute, Cleveland Clinic, Cleveland, OH, USA
GiovaNNi Nicolao berta • Department of Clinical and Biological Sciences, University
of Turin, Orbassano (Turin), Italy
Michele caraGlia • Department of Biochemistry, Biophysics and General Pathology,
Second University of Naples, Naples, Italy
Maria eliSabetta carere • Department of Clinical and Biological Sciences, University
of Turin, Orbassano (Turin), Italy
Silvia caroSio • Center for Life Nano Sciences—Sapienza, Instituto Italiano di Tecnologia,
Rome, Italy
MeliSSa carr-reyNoldS • Institute for Regenerative Engineering, University of Connecticut
Health Center, Farmington, CT, USA
zhoNGjiaN cheNG • Center for Translational Medicine, Temple University School of
Medicine, Philadelphia, PA, USA
SaNjiv dhiNGra • Institute of Cardiovascular Sciences, St-Boniface Hospital Albrechtsen
Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health
Sciences, University of Manitoba, Winnipeg, Manitoba, MB, Canada
Paolo di Nardo • Center for Regenerative Medicine, Università di Roma Tor Vergata,
Rome, Italy; Department of Clinical Sciences and Translational Medicine, Università di
Roma Tor Vergata, Rome, Italy
Federica di SciPio • Department of Clinical and Biological Sciences, University of Turin,
Orbassano (Turin), Italy
Ghada abu elGhaNaM • Craniofacial Tissue Engineering and Stem Cells Laboratory,
Faculty of Dentistry, McGill University, Montreal, QC, Canada; University of Jordan,
Amman, Jordan
doNGdoNG FaNG • Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty of
Dentistry, McGill University, Montreal, QC, Canada
ix
x Contributors
Federico FraNchi • Division of Cardiovascular Diseases, Department of Internal
Medicine, Mayo Clinic, Rochester, MN, USA
Mauro Giacca • Molecular Medicine Laboratory, International Centre for Genetic
Engineering and Biotechnology (ICGEB), Trieste, Italy; Department of Medical, Surgical
and Health Sciences, University of Trieste, Trieste, Italy
aNNa GriMaldi • Department of Biochemistry, Biophysics and General Pathology, Second
University of Naples, Naples, Italy
Ghazaleh hajMouSa • Cardiovascular Regenerative Medicine Research Group of the
Department of Pathology and Medical Biology, University of Groningen, University
Medical Center, Groningen, The Netherlands
MartiN c. harMSeN • Cardiovascular Regenerative Medicine Research Group of the
Department of Pathology and Medical Biology, University of Groningen, University
Medical Center Groningen, Groningen, The Netherlands
FraNkliN jebaraj herbert • Centre for Stem Cell Research, A Unit of inStem Bengaluru,
Christian Medical College, Bagayam, Vellore, Tamil Nadu, India
kiM b. jeNSeN • Biotech Research and Innovation Centre, University of Copenhagen,
Copenhagen, Denmark
taylor a. johNSoN • Division of Metabolic and Cardiovascular Sciences, Burnett School of
Biomedical Sciences, University of Central Florida, Orlando, FL, USA
darukeShwara joladaraShi • Department of Biomedical Engineering, University of
Alabama at Birmingham, Birmingham, AL, USA
jack jordaN • Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty of
Dentistry, McGill University, Montreal, QC, Canada
ShalMli u. joShi • Department of Biomedical Engineering, University of Connecticut,
Storrs, CT, USA
takako kawaSaki • Department of Regenerative Oral Surgery, Nagasaki University
Graduate School of Biomedical Sciences, Nagasaki, Japan
Saeed khalili • University of Toronto, Toronto, ON, Canada
MohSiN khaN • Center for Translational Medicine, Temple University School of Medicine,
Philadelphia, PA, USA
raj kiShore • Center for Translational Medicine, Temple University School of Medicine,
Philadelphia, PA, USA; Department of Pharmacology, Temple University School of
Medicine, Philadelphia, PA, USA
PraSaNNa kriShNaMurthy • Department of Biomedical Engineering, University of
Alabama at Birmingham, Birmingham, AL, USA
SaNjay kuMar • Centre for Stem Cell Research, A Unit of inStem Bengaluru, Christian
Medical College, Bagayam, Vellore, Tamil Nadu, India
juStiN d. lathia • Department of Cellular and Molecular Medicine, Lerner Research
Institute, Cleveland Clinic, Cleveland, OH, USA; Department of Molecular Medicine,
Cleveland Clinic Lerner College of Medicine of Case Western Reserve University,
Cleveland, OH, USA; Case Comprehensive Cancer Center, Cleveland, OH, USA
li-chieh liN • Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty
of Dentistry, McGill University, Montreal, QC, Canada
youNaN liu • Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty
of Dentistry, McGill University, Montreal, QC, Canada
hoNGhai liu • Department of Pathology and Laboratory Medicine, University of
Cincinnati Medical Center, Cincinnati, OH, USA
Contributors xi
aNGela loMbardi • Department of Biochemistry, Biophysics and General pathology, Second
University of Naples, Naples, Italy
kay Maeda • Division of Cardiac Surgery, University of Ottawa Heart Institute Ottawa,
Ottawa, ON, Canada
NicholaS Makhoul • Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty
of Dentistry, McGill University, Montreal, QC, Canada
Gabriella MiSSo • Department of Biochemistry, Biophysics and General Pathology, Second
University of Naples, Naples, Italy
kaSPer S. MoeStruP • Biotech Research and Innovation Centre, University of Copenhagen,
Copenhagen, Denmark
MeeNal MoudGil • Institute of Cardiovascular Sciences, St . Boniface Hospital Albrechtsen
Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health
Sciences, University of Manitoba, Winnipeg, MB, Canada
aNtoNio MuSarò • Instituto Pasteur-Fondazione Cenci Bolognetti, DAHFMO-Unit of
Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza
University of Rome, Rome, Italy; Center for Life Nano Science—Sapienza, Instituto
Italiano di Tecnologia, Rome, Italy
SyaM P. NukavaraPu • Department of Biomedical Engineering, University of Connecticut,
Storrs, CT, USA; Institute for Regenerative Engineering, University of Connecticut
Health Center, Farmington, CT, USA; Materials Science & Engineering, University of
Connecticut, Storrs, CT, USA; Department of Orthopedic Surgery,University of
Connecticut Health Center, Farmington, CT, USA
FraNceSca oltoliNa • Department of Health Sciences, Università del Piemonte Orientale
“A .Avogadro”, Novara, Italy
FraNceSca PaGliari • Center for Regenerative Medicine, Università di Roma Tor Vergata,
Rome, Italy; Biological and Environmental Science and Engineering, King Abdullah
University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia
chriStiaN Paul • Department of Pathology and Laboratory Medicine, University
of Cincinnati Medical Center, Cincinnati, OH, USA
huaN PhaM • Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty
of Dentistry, McGill University, Montreal, QC, Canada
Maria Prat • Department of Health Sciences, Università del Piemonte Orientale
“A .Avogadro”, Novara, Italy
Giulia ProSdociMo • Molecular Medicine Laboratory, International Centre for Genetic
Engineering and Biotechnology (ICGEB), Trieste, Italy
Murali raMaMoorthi • Craniofacial Tissue Engineering and Stem Cells Laboratory,
Faculty of Dentistry, McGill University, Montreal, QC, Canada
MartiN rodriGuez-Porcel • Division of Cardiovascular Diseases, Department of Internal
Medicine, Mayo Clinic, Rochester, MN, USA
Michael roSkieS • Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty of
Dentistry, McGill University, Montreal, QC, Canada; Department of Otolaryngology—
Head and Neck Surgery, McGill University, Montreal, QC, Canada
Marc ruel • Division of Cardiac Surgery, University of Ottawa Heart Institute, Ottawa,
ON, Canada
vikraM SabaPathy • Centre for Stem Cell Research, A Unit of inStem Bengaluru,
Christian Medical College, Bagayam, Vellore, Tamil Nadu (TN), India