Microarray Technology: Methods and Applications

294 Pages·2016·11.284 MB·English

by  Paul C.H. Li, Abootaleb Sedighi, Lin Wang (eds.)

Methods in
Molecular Biology 1368
Paul C.H. Li · Abootaleb Sedighi
Lin Wang Editors
Microarray
Technology
Methods and Applications
M M B
ETHODS IN OLECULAR IOLOGY
Series Editor
John M. Walker
School of Life and Medical Sciences
University of Hertfordshire
Hat fi eld, Hertfordshire, AL10 9AB, UK
For further volumes:
http://www.springer.com/series/7651

Microarray Technology
Methods and Applications
Edited by
Paul C.H. Li
Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada
Abootaleb Sedighi
Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada
Lin Wang
Department of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada
Editors
Paul C .H. L i Abootaleb Sedighi
Department of Chemistry Department of Chemistry
Simon Fraser University Simon Fraser University
Burnaby, BC, Canada Burnaby, B C , Canada
Lin Wang
Department of Biomedical Engineering
University of British Columbia
Vancouver, BC, Canada
ISSN 1064-3745 ISSN 1940-6029 (electronic)
Methods in Molecular Biology
ISBN 978-1-4939-3135-4 ISBN 978-1-4939-3136-1 (eBook)
DOI 10.1007/978-1-4939-3136-1
Library of Congress Control Number: 2015952014
Springer New York Heidelberg Dordrecht London
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Dedication
Dedicated to Cindy Woo, Paul Chan who have encouraged and supported my quest for
analytical tools to fi ght cancer, in Australia and beyond.

Prefa ce
The microarray method has been a widely applied technology. There are considerable
amounts of technical and scientifi c literature published recently. The aim of this book is to
provide updates of this established technology in topics such as methods and applications,
as well as advances in applications of the microarray method to assay substances such as
proteins, glycans, and whole cells. The 19 chapters, which are written by recognized experts
in each topic, include reviews, detailed protocols and outlook of the nucleic acid microar-
rays and the cell/tissue microarrays.
Burnaby, BC, Canada Paul C. H. Li, Ph.D.
Vancouver, BC, Canada L in W ang, Ph.D.
Burnaby, BC, Canada Abootaleb Sedighi, Ph.D.
vii

Contents
Dedication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
PART I INTRODUCTION
1 Overview of Microarray Technology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Paul C . H . Li
PART II ADVANCES ON TECHNIQUES IN MICROARRAY TECHNOLOGY
2 Surface Functionalization for Immobilization of Probes on Microarrays. . . . . . 7
C. D esmet and C . A. M arquette
3 T he “Clickable” Method for Oligonucleotide Immobilization
Onto Azide-Functionalized Microarrays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Tomasz R atajczak , Barbara U szczyńska , E milia Frydrych-Tomczak ,
and Marcin K . Chmielewski
4 Microarray Developed on Plastic Substrates. . . . . . . . . . . . . . . . . . . . . . . . . . . 3 7
María-José B añuls , Sergi B . M orais , Luis A. Tortajada-Genaro ,
and Ángel M aquieira
5 D etection and Quantification of MicroRNAs by Ligase-A ssisted
Sandwich Hybridization on a Microarray. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Ryo Iizuka , T aro U eno , and Takashi F unatsu
6 P robe Design Strategies for Oligonucleotide Microarrays . . . . . . . . . . . . . . . . 6 7
N icolas Parisot , E ric P eyretaillade , E ric D ugat-Bony ,
Jérémie D enonfoux , Antoine M ahul , and P ierre Peyret
PART III APPLICATIONS OF MICROARRAY TECHNOLOGY
TO NUCLEIC ACID ASSAYS
7 Analyzing Illumina Gene Expression Microarray Data Obtained
From Human Whole Blood Cell and Blood Monocyte Samples. . . . . . . . . . . . 8 5
Alexander Teumer , C laudia Schurmann , A rne Schillert ,
Katharina Schramm , A ndreas Ziegler , and Holger Prokisch
8 M icroarray Technology and Its Applications for Detecting Plasma
microRNA Biomarkers in Digestive Tract Cancers. . . . . . . . . . . . . . . . . . . . . . 99
Hirotaka K onishi , Daisuke I chikawa , Tomohiro Arita , and Eigo O tsuji
9 A pplication of DNA Microarray to Clinical Diagnostics. . . . . . . . . . . . . . . . . . 1 11
Ankita Patel and S au W. C heung
ix