IS 13426: Animal feeds and feeding stuffs - Methods of sampling for aflatoxin analysis

11 Pages·1992·1.1 MB·English

by  Bureau of Indian Standards

इंटरनेट मानक
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Whereas the Parliament of India has set out to provide a practical regime of right to
information for citizens to secure access to information under the control of public authorities,
in order to promote transparency and accountability in the working of every public authority,
and whereas the attached publication of the Bureau of Indian Standards is of particular interest
to the public, particularly disadvantaged communities and those engaged in the pursuit of
education and knowledge, the attached public safety standard is made available to promote the
timely dissemination of this information in an accurate manner to the public.
“जान1 का अ+धकार, जी1 का अ+धकार” “प0रा1 को छोड न’ 5 तरफ”
Mazdoor Kisan Shakti Sangathan Jawaharlal Nehru
“The Right to Information, The Right to Live” “Step Out From the Old to the New”
IS 13426 (1992): Animal feeds and feeding stuffs - Methods
of sampling for aflatoxin analysis [FAD 5: Livestock Feeds,
Equipment and Systems]
“!ान $ एक न’ भारत का +नम-ण”
Satyanarayan Gangaram Pitroda
““IInnvveenntt aa NNeeww IInnddiiaa UUssiinngg KKnnoowwlleeddggee””
“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता हहहहै””ै”
Bhartṛhari—Nītiśatakam
“Knowledge is such a treasure which cannot be stolen”

IS 62431 : 1992

ANIMAL FEEDS AND FEEDING STUFFS-
METHODS OF SAMPLING FOR AFLATOXIN
ANALYSiS
UDC 636.085 : 543.05 [ 615.9 ]

@ BIS 1992
BUREAU OF INDIAN STANDARDS
MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG
NEW DELHI 110002
May 1992 Price Group 2
kcotseviL sdeeF lanoitceS ,eettimmoC DAF 5
DROWEROF
sihT naidnI dradnatS was adopted yb the uaeruB of naidnI ,sdradnatS after the draft dezilanif yb
the kcotseviL sdeeF lanoitceS eettimmoC had neeb devorppa yb the dooF dna erutlucirgA
noisiviD .licnuoC
gnilpmaS has a yrev lacitirc elor ot yalp ni the sisylana of elbarisednu ,scitsiretcarahc hcus as
,snixotada ylekil ot eb non ylmrofinu detubirtsid ni lamina feeds dna gnideef .sffuts nI order ot
ediced whether a tol seilpmoc htiw the mumixam stimil for nixotalfa debircserp ni the tnaveler
naidnI ,dradnatS ti si yrassecen that the elpmas nwa-rd si evitatneserper of a .tol
sihT naidnI dradnatS si desab no 76/371 EEC tsriF noissimmoC evitceriD of I March 1976 -atse
gnihsilb ytinummoC sdohtem of gnilpmas for the laiciffo lortnoc of gnideef sffuts ( OJ L 102
15.04. 76 ). ylnO the gnilpmas erudecorp for elbarisednu scitsiretcarahc has neeb adopted ni siht
.dradnats ehT noitatneserp of selbaT has neeb deifilpmis ot etatilicaf sti .esu
roF the esoprup of gnidiced whether a ralucitrap tnemeriuqer of siht dradnats si deilpmoc ,htiw
the lanif ,eulav devresbo or ,detaluclac gnisserpxe the tluser of a test or ,sisylana llahs eb dednuor
off ni ecnadrocca htiw SI 2 : 1960 seluR‘ for gnidnuor off !aciremun seulav ( revised )‘. ehT
rebmun of tnacifingis secalp deniater ni the dednuor off eulav dluohs eb the emas as that of the
deificeps eulav ni siht .dradnats
IS 13426 : 1992
Indian Standard
ANIMAL FEEDS AND FEEDING STUFFS-
METHODS OF SAMPLING FOR AFLATOXIN
ANALYSIS
4
1 SCOPE SAMPLING REQUIREMENTS
sihT dradnatS sebircserp eht sdohtem fo samp- 4.1 Loose Feeding Stuffs
gnil rof animal sdeef and gnideef sYfuts ni -aler
noit ot eht stnemeriuqer fo elbarisednu -carahc
4.1.1 Minimum Number of Increments
scitsiret ylekil ot eb detubirtsid ylmrofinu-non
tuohguorht eht gnideef ,sffuts hcus as .snixotalfa nehW stol era ni eht mrof fo esool gnideef
,sffuts stnemercni shall eb drawn morf hcae tol
2 ni ecnadrocca htiw 6.2. ehT muminim rebmun
REFERENCES
fo stnemercni ot eb nekat morf a tol sdneped
The gniwollof naidnI dradnatS is a yrassecen nopu eht tol ezis and shall eb ni ecnadrocca
tcnujda ot this standard: htiw 1oc 2 and 3 fo elbaT 1.
IS No. Title 4.1.2 Minimum Mass of Each Increment
4905 : 1968 sdohteM rof modnar sampling ehT muminim mass fo hcae tnemercni shall eb
as deificeps ni 1oc 4 fo elbaT 1.
3
TERMINOLOGY
4.1.3
Minimum Number of Aggregate Samples
roF eht esoprup fo this ,dradnatS eht
3.0 etagerggA samples shall eb deraperp ni -rocca
gniwollof snoitinifed shall .ylppa
ecnad htiw 6.3. ehT muminim rebmun fo
etagergga samples lliw yrav htiw eht ezis fo eht
3.1
Lot tol and is deificeps ni 1oc 5 fo elbaT 1.
ehT ytitnauq fo ffutsgnideef fo a ralucitrap epyt
4.1.4
decudorp rednu ylevitaler ralimis snoitidnoc Minimum Mass of Each Aggregate #Sample
shall etutitsnoc a .tol
ehT muminim mass fo hcae etagergga elpmas
ETON - ylevitaleR ralimis snoitidnoc dluow naem eht shall ton eb less than 4 kg.
esu fo raw slairetam gnivah tnacifingisni ,snoitairav
ralimis snoitidnoc fo ,erutcafunam .cte 4.2 Packaged Feeding Stuffs
3.2 Increment 4.2.1 Minimum Number of Packages in a Sample
A ytitnauq fo lairetam nekat at eno tniop morf nehW stol era ni eht mrof fo ,segakcap eht
eht .tol muminim rebmun fo segakcap ot eb nekat morf
hcae tol sdneped nopu eht tol ezis and shall eb
3.3
Aggregate Sample ni ecnadrocca htiw 1oc 2 and 3 fo elbaT 2.
esehT segakcap ni eht eIpmas shall eb drawn
etage;;T;mz fo stnemercni nekat morf eht
morf eht tol at .modnar roF eht ,esoprup -efer
ccncr shall eb edam ot SI 4905 : 1968.
3.4
Reduced Sample
4.2.2
Minimum Mass of Each Increment
A evitatneserper trap fo eht etagergga ,elpmas
stnemercnI shall cb drawn morf hcae fo eht
deniatbo morf eht rettal yb a ssecorp fo -cuder
detceles segakcap ni eht .elpmas roF this -rup
.noit
,esop a trap f>c eht stnetnoc fo eht delpmas
egakcap shall eb devomer retfa ,gnivah fi -secen
3.5
Final Sample ,yras dcitpmc eht segakcap .yletarapes ehT
A trap fo eht decuder elpmas ro fo eht -omoh muminim rebmun fo hcae tnemercni shall cb ni
dezineg etagergga .elpmas ecnadrocca htiw 1oc 4 fo elbaT 2.
1
.
IS 13426: 1992
Table 1 Loose Feeding Stuffs
( Clause 4.1 )
IS Lot Size (tonnes) Minimum Number Minimum Mass of Aggregate Samples
No. of Increments Each Increment (g)
~~~_~___*~_~~__~
No. ssaM Total
(kg) )sk(
(1) (2, (3) (4) (5) (6) (7)
)i up ot 1 7 600 1 4 4
)ii 1 ot 3 8 1000 2 4 8
)iii 3 ot 4 9 I 000 2 4 8
)vi 4 ot 5 10 800 2 4 8
“J 5 ot 6 11 800 2 4 8
)iv 6to7 12 800 2 4 8
vii) 7 ot 8 13 800 2 4 8
)iiiv 8 ot 9 14 800 2 4 8
)xi 9 to 10 15 800 2 4 8
x) 10 to 20 20 600 3 4 12
xi) 20 ot 04 30 600 3 4 12
xii) 40 dna evoba 40 400 4 4 16
Table 2 Packaged Feeding Stuffs
( Clause 4.2 )
SI No. Lot Size (Packages of Minimum Number of Minimum Mass of Aggregate Samples
50 kg and Above) Packages in Sample each Increment (kg)
C-L- ------C-_--__~
No. ssaM Total
(kg) (kg)
(1) (2) (3) (4) (5) (6) (7)
9 1 to 4 llA 4
)ii 5 ot 16 4 8
}iii 17 to 25 5 8
)vi 26 ot 36 6 8
)v 37 ot 49 7 8
)iv 50 ot 196 ot8 14 8
jiiv 197 ot 529 15 ot 23 12
)iiiv 530 ot 844 24 ot 29 12
)xi 845 ot 1600 30 o: 40 16
NOTE - roF 101 ezis erom naht 50 ,segakcap eht muminim rebmun fo segakcap ot eb detceles ni a elpmas si
detaluclac gnisu eht gniwollof alumrof :
JNumber of segakcap gnikam a lot ezis
( otpu a mumixam fo 40 segakcap )
4.2.3 Minimum Number of Aggregate Samples 4.3 Minimum Mass of Final Samples
etagerggA samples shall eb deraperp ni -rocca
ecnad htiw ehT muminim rebmun fo -ergga hcaE etagergga elpmas 5evig eht lanif samples
6.3.
etag samples lliw yrav htiw eht ezis fo eht tol no noitcuder ( ees 5 eerhT lanif
and 4.4 ).
and shall eb as deificeps ni 1oc 5 fo elbaT 2. samples eta deriuqer ot eb deraperp morf hcae
etagergga .elpmas sisylanA fo at tsael eno lanif
4.2.4
Minimum Mass of Each Aggregate Sample elpmas rep etagergga elpmas is .deriuqer ehT
ehT muminim mass fo hcae etagergga elpmas mass fo eht lanif elpmas rof sisylana shall ton
shall ton eb less than 4 kg. eb less than 500 g.
2
3S 13426 : 1992
sampling tnemurtsni and eht sreniatnoc rof
5 REDUCTION OF AGGREGATE SAMPLE
samples morf suoititnevda .noitanimatnoc
hcaE etagergga elpmas shall eb decuder -apes
yletar yb rehtie fo eht gniwollof .sdohtem 6.1.6 ehT samples shall eb decalp ni naelc and
yrd glass .sreniatnoc ehT elpmas sreniatnoc
5.1 Coning and Quartering Method shall eb of hcus a ezis that yeht era tsomla
yletelpmoc dellif yb eht .elpmas
ehT lairetam shall eb llew dexim and neht
depoocs otni a enoc depahs .elip eraC shall 6.1.7 hcaE reniatnoc shall eb delaes thgit-ria
eb nekat ot pord hcae lufpoocs yltcaxe revo htiw a reppots ro a elbatius erusolc retfa gnillif
eht emas tops as esiwrehto eht lartnec sixa fo ni hcus a yaw that ti is ton elbissop ot nepo
eht enoc lliw eb denekcals and an nevenu -irtsid and laeser ti tuohtiw ,noitceted and dekram
noitub fo eht lairetam lliw .tluser retfA eht htiw lluf sliated fo sampling, etad fo sampling
enoc is ,demrof ti shall eb denettalf yb gnisserp hctab ro edoc ,rebmun eman fo eht lutcafunam
eht pot fo eht enoc htiw eht htooms ecafrus fo rer and rehto tnatropmi sralucitrap fo eht
eht .poocs nehT ti is tuc otni sretrauq yb owt .tnemngisnoc
senil hcihw tcesretni at thgir selgna at eht
6.1.8 selpmaS shall eb derots ni hcus a rennam
ertnec fo eht .enoc ehT klub fo eht elpmas is
that ereht is on noitaroireted fo eht .lairetam
decudortni yb gnitcejer yna owt yllanogaid
etisoppo .sretrauq
6.2 Preparation of Increment Samples
5.2 Increment Reduction Method 6.2.1 An yranigami noisivid shall eb edam fo
eht tol otni a rebmun fo yletamixorppa lauqe
ehT lairetam shall eb daerps no a htooms -non
parts, gnidnopserroc ot eht rebmun fo -ergga
erutsiom gnibrosba etalp otni a mrofinu talf
etag samples dedivorp rof ni 1oc 5 fo selbaT I
elgnatcer fo elbatius .ssenkciht ehT elgnatcer
and 2 rof esool gnideef sffuts and degakcap
os demrof shall eb dedivid otni 5 lauqe parts
gnideef ,sffuts .ylevitcepser
esiwhtgnel and 4 lauqe parts .esiwhtdaerb morF
hcae fo eht 20 parts os ,deniatbo lauqe -itnauq
6.2.2 fI rebmun is retaerg than ,eno eht
seit fo lairetam shall eb detcelloc yb eht esu fo this
latot rebmun fo stnemercni dedivorp rof ni 1oc
elbatius poocs seno ro erom rebmun fo semit
3 fo selbaT 1 and 2 shall eb detubirtsid -ixorppa
gnidneped eht ytitnauq fo dedivid .elpmas
yletam yllauqe revo eht tnereffid parts.
6 INSTRUCTIONS FOR DRAWING, 6.2.3 nehT samples fo yletamixorppa lauqe
PREPARING AND PACKAGING OF sezis shall eb nekat hcus that eht latot tnuoma
SAMPLES ni eht samples morf hcae trap is ton less than
eht muminim 4 kg ytitnauq deriuqer rof hcae
nI ,gniward ,gniraperp gnirots and handling
etagergga .elpmas sihT tnuoma fo tnemercni
samples, erac dluohs eb nekat that eht -reporp
has neeb deificeps ni 1oc 4 fo selbaT 1 and 2,
seit fo eht lairetam era ton .detceffa ehT
gniwollof snoituacerp and snoitcerid shall eb .ylevitcepser
.devresbo 6.2.4 stnemercnI nekat morf tnereffid parts
shall ton eb .detagergga
6.1 lareneG
6.1.1 ehT sampling apparatus shall eb naelc 6.3 Preparation of Aggregate Samples
and yrd nehw .desu
ehT tnemercni morf hcae trap fo eht tol shall
eb dexim and eht rebmun fo etagergga samples
6.1.2 ehT sampling apparatus shall eb edam fo
dedivorp rof ni 1oc 5 fo selbaT 1 and 2 shall eb
slairetam hcihw tonnac etanimatnoc eht -orp
edam up, taking erac ot eton eht nigiro fo hcae
stcud ot eb .delpmas
etagergga .elpmas
6 dluohs .1.3 demottob-talF eb desu rof levohs sampling htiw esool lacitrev gnideef sides 6.4 Preparation of Final Samples
.sffuts
6.4.1 ehT lairetam ni each aggregate elpmas
llahs eb ylluferac dexim ot niatbo an -egomoh
6.1.4 gnilpmaS poocs htiw etairporppa gnol
dezin .elpmas fI yrassecen eht etagergga sam-
split dluohs eb desu rof sampling degakcap -deef
elp dluohs tsrif eb decuder ot at tsacI 2 kg
gni -sffuts
decuder( )elpmas rehtie yb using eht gninoc
6.1.5 snoituacerP shall eb nekat ot tcetorp eht and gniretrauq dohtem ro eht tnemercni -cuder
samples, eht lairetam gnieb ,delpmas eht noit dohtem ( ees 5 .)
3
IS 13426: 1992
6.4.2 tA tsael three lanif selpmas of muminim 5W6 gnigakcaP Of aniP se’pmaS
500 g each llahs neht eb prepared, morf each ehT sreniatnoc of lanif selpmas llahs eb delaes
aggregate elpmas ( see 4.3). Each elpmas llahs dna dellebal ni hcus a rennam that yeht -nac
eb tup otni na etairporppa .reniatnoc llA ton eb denepo tuohtiw gnigamad the .laes
yrassecen snoituacerp llahs eb nekat ot diova
7 GNITROPER FO TSET STLUSER
yna egnahc of noitisopmoc of the ,elpmas
noitanimatnoc or noitaretluda hcihw thgim laniF selpmas llahs eb tested for nixotalfa -noc
esira gnirud noitatropsnart or storage. tnet dna the test stluser llahs eb reported as
the eulav of nixotalfa for the .tol
-
Standard Mark
ehT esu of the dradnatS Mark si denrevog yb the snoisivorp of the Bureau oj Indian
Standards Act, 1986 dna the seluR dna snoitalugeR edam .rednuereht ehT dradnatS Mark
no stcudorp derevoc yb na naidnI dradnatS syevnoc the ecnarussa that yeht evah neeb
decudorp ot ylpmoc htiw the stnemeriuqer of that dradnats rednu a llew denifed metsys
of ,noitcepsni gnitset dna ytilauq lortnoc hcihw si desived dna desivrepus yb SIB dna
operated yb the .recudorp dradnatS dekram stcudorp are osla ylsuounitnoc checked yb
SIB for ytimrofnoc ot that dradnats as a rehtruf .draugefas sliateD of snoitidnoc rednu 1
hcihw a ecnecil for the esu of the dradnatS Mark yam eb detnarg ot srerutcafunam or ~
srecudorp yam eb deniatbo morf the uaeruB of naidnI .sdradnatS