Hepatocyte Transplantation: Methods and Protocols PDF
Preview Hepatocyte Transplantation: Methods and Protocols
Hepatocyte Transplantation M E T H O D S I N M O L E C U L A R B I O L O G Y TM John M. Walker, SERIES EDITOR 502. Bacteriophages:MethodsandProtocols,Volume2:Mole- 467. Angiogenesis Protocols: Second Edition, edited by cularandAppliedAspects,editedbyMarthaR.J.Clokie StewartMartinandCliffMurray,2008 andAndrewM.Kropinski2009 466. Kidney Research: Experimental Protocols, edited by 501. Bacteriophages:MethodsandProtocols,Volume1:Isola- TimD.HewitsonandGavinJ.Becker,2008 tion,Characterization,andInteractions,editedby 465. Mycobacteria,SecondEdition,editedbyTanyaPar- MarthaR.J.ClokieandAndrewM.Kropinski2009 ishandAmandaClaireBrown,2008 496. DNAandRNAProfilinginHumanBlood:Methods 464. TheNucleus,Volume2:PhysicalPropertiesandIma- andProtocols,editedbyPeterBugert,2009 gingMethods,editedbyRonaldHancock,2008 493. AuditoryandVestibularResearch:MethodsandProto- 463. TheNucleus,Volume1:NucleiandSubnuclearCom- cols,editedbyBerndSokolowski,2009 ponents,editedbyRonaldHancock,2008 490. 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CancerEpidemiology,Volume1:HostSusceptibility FunctionalProteomics,SecondEdition,editedby Factors,editedbyMukeshVerma,2008 ChristophKannicht,2008 470. Host-Pathogen Interactions: Methods and Protocols, 445. Autophagosome and Phagosome, edited by Vojo editedbySteffenRuppandKaiSohn,2008 Deretic,2008 469. WntSignaling,Volume2:PathwayModels,editedby 444. PrenatalDiagnosis,editedbySinhueHahnandLaird ElizabethVincan,2008 G.Jackson,2008 468. Wnt Signaling, Volume 1: Pathway Methods and 443. MolecularModelingofProteins,editedbyAndreas MammalianModels,editedbyElizabethVincan,2008 Kukol,2008 M M B TM E T H O D S I N O L E C U L A R I O L O G Y Hepatocyte Transplantation Methods and Protocols Edited by Anil Dhawan King’s College Hospital, London, UK Robin D. Hughes King’s College London, School of Medicine London, UK Editors AnilDhawan RobinD.Hughes King’sCollegeHospital King’sCollegeLondon London,UK SchoolofMedicine [email protected] London,UK [email protected] SeriesEditor JohnM.Walker UniversityofHertfordshire Hatfield,Herts. UK ISBN:978-1-58829-883-6 e-ISBN:978-1-59745-201-4 ISSN:1064-3745 e-ISSN:1940-6029 DOI10.1007/978-1-59745-201-4 LibraryofCongressControlNumber:2008939645 #HumanaPress,apartofSpringerScienceþBusinessMedia,LLC2009 Allrightsreserved.Thisworkmaynotbetranslatedorcopiedinwholeorinpartwithoutthewrittenpermissionofthe publisher(HumanaPress,c/oSpringerScience+BusinessMedia,LLC,233SpringStreet,NewYork,NY10013,USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of informationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodology nowknownorhereafterdevelopedisforbidden. Theuseinthispublicationoftradenames,trademarks,servicemarks,andsimilarterms,eveniftheyarenotidentified assuch,isnottobetakenasanexpressionofopinionastowhetherornottheyaresubjecttoproprietaryrights. Coverillustration:Figure1fromchapter15 Printedonacid-freepaper 9 8 7 6 5 4 3 2 1 springer.com Preface Cellular therapy using human hepatocytes is being evaluated worldwide as an alternativetoorgantransplantationinpatientswithliver-basedmetabolicdiseaseand acuteliverfailure.Thebasisforclinicalusehascomefromthedemonstrationofefficacy inanimalmodelsofacuteandchronicliverdisease. Protocols have been developed for the isolation of hepatocytes from liver tissue under GMP conditions and also for improved methods of cryopreservation, so hepa- tocytes can be stored for later clinical use. Assays are used to assess the quality and function of the hepatocytes prior to transplantation. There are clinical protocols for administrationofcellsdirectlyintothepatient’sliver. The engraftment of donor cells in the recipient liver can be detected by DNA techniquesorfunctionalproteinsinthecaseofgeneticliverdisorders.Invivomethods areneededtotrackthefateofhepatocytesaftertransplantation. Due to the shortage of donor organs,thefuture of hepatocyte transplantation will be alternative sources of liver cells such as foetal hepatoblasts or stem cell-derived hepatocytes. Methods for culture and in vitro proliferation of stem cells will be importantfortheirapplication. Itishopedthatthisvolumefromtheexpertsinthefieldprovidesthereaderwiththe practicalprotocolstoenablethemtoperformandinvestigatehepatocytetransplanta- tion. Needless to say this is a rapidly developing field, and new and improved techni- quesarebeingdevelopedallthetime. AnilDhawan&RobinD.Hughes v Acknowledgements To my wife Anita and boys Atin and Ashish for their understanding, love and supportthattheyhaveprovidedthroughoutmycareer. Sincerethankstoallthecontributors. Particular thanks to Professor Nigel Heaton, Mr Mohamed Rela, Liver Transplant Coordinators,andDrRagaiMitryforhelpingestablishthehepatocytetransplantation programmeatKing’sCollegeHospital. AnilDhawan vii Contents Preface..............................................................v Acknowledgements ....................................................vii Contributors......................................................... xi ColorPlates .........................................................xv 1 HumanHepatocyteTransplantationOverview ...........................1 JulianaPuppiandAnilDhawan 2 IsolationofHumanHepatocytes.....................................17 RagaiR.Mitry 3 AnOptimisedMethodforCryopreservationofHumanHepatocytes .........25 ClaireTerryandRobinD.Hughes 4 LiverCellCultureTechniques.......................................35 Jose´ V.CastellandMarı´aJose´ Go´mez-Lecho´n 5 InVitroAssaysforInductionofDrugMetabolism .......................47 BrianG.Lake,RogerJ.Price,AmandaM.Giddings, andDavidG.Walters 6 HepatocyteApoptosis .............................................59 MustaphaNajimi,Franc¸oiseSmets,andEtienneSokal 7 SmallAnimalModelsofHepatocyteTransplantation .....................75 JurgenSeppen,EbtisamElFilali,andRonaldOudeElferink 8 HepatocyteTransplantationTechniques:LargeAnimalModels .............83 AnneWeber,Marie-The´r`eseGroyer-Picard,andIbrahimDagher 9 CellTransplantTechniques:EngraftmentDetectionofCells ...............97 RobertA.FisherandValeriaR.Mas 10 HepaticPreconditioningforTransplantedCellEngraftment andProliferation ................................................107 Yao-MingWuandSanjeevGupta 11 ExVivoGeneTransferintoHepatocytes..............................117 XiaWang,PrashantMani,DebiP.Sarkar,NamitaRoy-Chowdhury, andJayantaRoy-Chowdhury 12 SourcesofAdultHepaticStemCells:Haematopoietic ...................141 RosemaryJeffery,RichardPoulsom,andMalcolmR.Alison 13 ProductionofHepatocyte-LikeCellsfromHumanAmnion...............155 ToshioMiki,FabioMarongiu,EwaC.S.Ellis,KenDorko, KeitaroMitamura,AaratiRanade,RobertoGramignoli, JulioDavila,andStephenC.Strom 14 GenerationofHepatocytesfromHumanEmbryonicStemCells ...........169 NiloufarSafiniaandStephenLMinger ix x Contents 15 Isolation,InVitroCultivationandCharacterisationofFoetal LiverCells .....................................................181 YueWu,ChetanC.Shatapathy,andStephenL.Minger 16 HumanIntrahepaticBiliaryEpithelialCellLineages:StudiesInVitro .......193 RuthJoplinandStiveliaKachilele 17 LiverCellLabellingwithMRIContrastAgents ........................207 MichelModo,ThomasJ.Meade,andRagaiR.Mitry 18 MicrobiologicalMonitoringofHepatocyteIsolationintheGMP Laboratory.....................................................221 SharonC.Lehec Index.............................................................229 Contributors MALCOLMR.ALISON.CentreforDiabetesandMetabolicMedicine,ICMS,Bart’sand TheLondonSchoolofMedicine,London,UK JOSE´ V.CASTELL.UnitofExperimentalHepatology,UniversityHospital‘‘LaFe’’, Valencia,Spain IBRAHIMDAGHER.InsermU804;UniversityParis-Sud,HoˆpitaldeBiceˆtre, Kremlin-Biceˆtre,andServicedeChirurgieGe´ne´rale,HoˆpitalBe´cl`ere,Clamart, France JULIODAVILA.Pfizer,Inc.,St.LouisMo,USA ANILDHAWAN.PaediatricLiverCentre,King’sCollegeHospital,DenmarkHill, London,UK EBTISAMELFILALI.AMCLivercenter,Amsterdam,TheNetherlands KENDORKO.DepartmentsofPathologyandSurgeryandMcGowanInstitute forRegenerativeMedicine,UniversityofPittsburgh,USA EWAC.S.ELLIS.DepartmentsofPathologyandSurgeryandMcGowanInstitute forRegenerativeMedicine,UniversityofPittsburgh,USA ROBERTA.FISHER.DepartmentofSurgery,TransplantationDivision,Virginia CommonwealthUniversity,MedicalCollegeofVirginiaHospitals,Richmond, Virginia,USA DOMINIQUEFRANCO.InsermU804;UniversityParis-Sud,HoˆpitaldeBiceˆtre, Kremlin-Bicˆetre,andServicedeChirurgieGe´ne´rale,HoˆpitalBe´cl`ere,Clamart,France AMANDAM.GIDDINGS.BIBRAInternational,Carshalton,SurreyandCentrefor Toxicology,FacultyofHealthandMedicalSciences,UniversityofSurrey,Guildford, UK MAR´IAJOSE´ GO´MEZ-LECHO´N.UnitofExperimentalHepatology,UniversityHospital‘‘La Fe’’,Valencia,Spain ROBERTOGRAMIGNOLI.DepartmentsofPathologyandSurgeryandMcGowanInstitute forRegenerativeMedicine,UniversityofPittsburgh,USA MARIE-THE´RE`SEGROYER-PICARD.InsermU804;UniversityParis-Sud,HoˆpitaldeBiceˆtre, Kremlin-Biceˆtre,France SANJEEVGUPTA.MarionBessinLiverResearchCenter,DiabetesCenter,CancerResearch Center,DepartmentsofMedicineandPathology,andInstituteforClinicaland TranslationalResearch,AlbertEinsteinCollegeofMedicine,NewYork,USA ROBIND.HUGHES.InstituteofLiverStudies,King’sCollegeLondonSchoolofMedicine, London,UK ROSEMARYJEFFERY.HistopathologyUnit,CancerResearchUK,LondonResearch Institute,London,UK RUTHJOPLIN.LiverResearchLaboratories,InstituteofBiomedicalResearch,University ofBirminghamMedicalSchool,Birmingham,UK xi
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