Epiblast Stem Cells: Methods and Protocols PDF
Preview Epiblast Stem Cells: Methods and Protocols
Methods in Molecular Biology 1074 Ramiro Alberio Editor Epiblast Stem Cells Methods and Protocols M M B ™ ETHODS IN OLECULAR IOLOGY Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hat fi eld, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 Epiblast Stem Cells Methods and Protocols Edited by Ramiro Alberio School of Biosciences, University of Nottingham, Loughborough, UK Editor Ramiro Alberio School of Biosciences University of Nottingham Loughborough, UK ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-62703-627-6 ISBN 978-1-62703-628-3 (eBook) DOI 10.1007/978-1-62703-628-3 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 2013945871 © Springer Science+Business Media New York 2 013 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifi cally the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfi lms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifi cally for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer. Permissions for use may be obtained through RightsLink at the Copyright Clearance Center. Violations are liable to prosecution under the respective Copyright Law. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specifi c statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is a brand of Springer Springer is part of Springer Science+Business Media (www.springer.com) Pref ace The cells of the epiblast give rise to all the tissues of the embryo proper. How epiblast cells acquire pluripotent features and how they transition to form the trilaminar embryo are cur- rent topics of intense investigation. A more detailed understanding of these in vivo events will lead to improved strategies for in vitro cellular differentiation protocols and genetic modifi cation. In E piblast Stem Cells : M ethods and Protocols , leading researchers provide a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast. As embryo development varies in model organisms, this volume includes chapters describing techniques used to study epiblast development in different amniotes. This collection brings contributions from the fi elds of embryology, stem cell biology and developmental biology together, such that readers can fi nd in a single volume detailed procedures for the isolation and culture of epiblasts at different stages of develop- ment and techniques for the study of differentiation into specifi c lineages. Over the past fi ve years, advances in the molecular characterization of pluripotent cells have led to a new understanding of how metastable states of pluripotency develop during the transition between the early inner cell mass to the late epiblast stage. Exploiting this new knowledge has enabled researchers to develop protocols that capture these novel pluripotent interme- diates. These intermediate stages of pluripotency are important for modelling the transi- tions that these cells undergo in vivo and will contribute to improving the development of robust in vitro differentiation protocols. Written in the highly successful M ethods in Molecular Biology ™ series format, chapters include introductions to their respective topics, a complete list of the necessary materials and reagents, detailed laboratory protocols, and extensive notes providing suggestions on troubleshooting and how to overcome common diffi culties. I hope the chapters presented in this volume will serve as an up-to-date source of novel protocols developed as a result of the new understanding in pluripotency and will contrib- ute to the incremental use of stem cell technologies in different species. Comprehensive and cutting-edge E piblast Stem Cells : M ethods and Protocols serves as a resource to individuals interested in studying the biology of pluripotent cells. Loughborough, UK Ramiro Alberio Contents Preface ............................................................................................................................. v Contributors .................................................................................................................... ix 1 Generation and Characterization of Epiblast Stem Cells from Blastocyst-Stage Mouse Embryos ............................................................. 1 Daniel C. Factor, Fadi J. Najm, and Paul J. Tesar 2 Reversion of Mouse Postimplantation Epiblast Stem Cells to a Naïve Pluripotent State by Modulation of Signalling Pathways ................... 15 Astrid Gillich, Siqin Bao, and M. Azim Surani 3 Converting Mouse Epiblast Stem Cells into Mouse Embryonic Stem Cells by Using Small Molecules ................................................................................. 31 Wenlin Li and Sheng Ding 4 Isolation and Culture of Rabbit Embryonic Stem Cells ..................................... 39 Arata Honda 5 Goat Embryonic Stem-Like Cell Derivation and Characterization ..................... 51 Esmail Behboodi, Louise Lam, William G. Gavin, Alla Bondareva, and Ina Dobrinski 6 Derivation and Culture of Canine Embryonic Stem Cells .................................. 69 Ian C. Tobias, Courtney R. Brooks, Jonathan H. Teichroeb, and Dean H. Betts 7 Isolation and Culture of Porcine Embryonic Stem Cells .................................... 85 Ivan Vassiliev and Mark B. Nottle 8 Isolation and Culture of Pig Epiblast Stem Cells ............................................... 97 Aida Rodriguez, David A. Contreras, and Ramiro Alberio 9 Isolation and Culture of Bovine Embryonic Stem Cells ..................................... 111 Shanbo Cao, Fang Wang, and Lin Liu 10 Analysis of Molecular Markers for Staging Peri-g astrulating Bovine Embryos ................................................................................................. 125 Isabelle Hue, Severine A. Degrelle, and Christoph Viebahn 11 Chicken Embryonic Stem Cells: Establishment and Characterization ................. 137 Pauline Aubel and Bertrand Pain 12 Manipulating the Avian Epiblast and Epiblast-Derived Stem Cells ..................... 151 Cantas Alev, Mikiharu Nakano, Yuping Wu, Hiroyuki Horiuchi, and Guojun Sheng viii Contents 13 Stepwise Differentiation from Naïve State Pluripotent Stem Cells to Functional Primordial Germ Cells Through an Epiblast-Like State ................ 175 Katsuhiko Hayashi and Mitinori Saitou 14 Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells ........................................................ 185 Mikkel A. Rasmussen, Vanessa J. Hall, and Poul Hyttel 15 Derivation of Neural Precursors from Bovine Preimplantation Embryos ............ 199 Giovanna Lazzari, Silvia Colleoni, and Cesare Galli Index ............................................................................................................................... 209 Contributors RAMIRO ALBERIO • Division of Animal Sciences, School of Biosciences, University of Nottingham , Loughborough, UK CANTAS ALEV • Laboratory for Early Embryogenesis, RIKEN Center for Developmental Biology , Chuo-Ku, Kobe, Japan PAULINE AUBEL • Inserm, U846, Stem Cell and Brain Research Institute , Bron , France ; Université de Lyon , Lyon , France SIQIN BAO • Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge , Cambridge, UK ESMAIL BEHBOODI • Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary , Calgary , AB , Canada DEAN H. BETTS • Department of Physiology and Pharmacology , Schulich School of Medicine and Dentistry, University of Western Ontario , London, ON , Canada ; Children’s Health Research Institute, Lawson Health Research Institute , London, ON , Canada ALLA BONDAREVA • Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary , Calgary , AB , Canada COURTNEY R. BROOKS • Department of Physiology and Pharmacology , Schulich School of Medicine and Dentistry, University of Western Ontario , London, ON , Canada SHANBO CAO • The Third Affi liated Hospital of Guangzhou Medical University , Guangzhou, China SILVIA COLLEONI • Avantea, Laboratory of Reproductive Technologies , Cremona, Italy DAVID A. CONTRERAS • Division of Animal Sciences, School of Biosciences, University of Nottingham , Loughborough, UK SEVERINE A. DEGRELLE • INRA, UMR1198 Biologie du Développement et Reproduction , Jouy-en-Josas, France ; ENVA , Maisons Alfort, France ; Department of Immunology, INSERM, Institut Cochin , Paris , France SHENG DING • Department of Pharmaceutical Chemistry , Gladstone Institute of Cardiovascular Disease, University of California , San Francisco, CA , USA INA DOBRINSKI • Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary , Calgary , AB , Canada DANIEL C. FACTOR • Department of Genetics and Genome Sciences, Case Western Reserve University School of Medicine , Cleveland, OH , USA CESARE GALLI • Avantea, Laboratory of Reproductive Technologies , Cremona, Italy ; Department of Veterinary Medical Sciences, University of Bologna , Bologna , Italy ; Fondazione Avantea , Cremona, Italy WILLIAM G. GAVIN • rEVO Biologics , Framingham, MA , USA ASTRID GILLICH • Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge , Cambridge, UK VANESSA J. HALL • Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen , Frederiksberg C, Denmark
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