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Biotechnological applications of microalgae: biodiesel and value added products PDF

251 Pages·2013·15.35 MB·English
by  F Bux
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Clean TeChnology B u Biotechnological x Applications B of Microalgae i o t e Biodiesel and c h Value-Added Products n o Microalgae are an invaluable biomass source with potential uses that could l o lead to environmental and economic benefits for society. Biotechnological g Applications of Microalgae: Biodiesel and Value-Added Products i presents the latest developments and recent research trends with a c a focus on potential biotechnologically related uses of microalgae. It gives l an analysis of microalgal biology, ecology, biotechnology, and biofuel A production capacity as well as a thorough discussion of the value-added p products that can be generated from diverse microalgae. p Biotechnological The book provides a detailed discussion of microalgal strain selection l i for biodiesel production, a key factor in successful microalgal cultivation c and generation of desired biofuel products. It also describes microalagal a enumeration methods, harvesting and dewatering techniques, and the t i Applications design, and the pros and cons, of the two most common methods for o cultivation—open raceway ponds and photobioreactors. Chapters cover n lipid extraction and identification, chemical and biological methods for s transesterification of microalgal lipids, and procedures involved in o of Microalgae life cycle analysis of microalgae. They also examine the importance f of microalgal cultivation for climate change abatement through CO M 2 sequestration and microalgae involvement in phycoremediation of domestic i and industrial wastewaters. c Biodiesel and r The book concludes with a general discussion of microalgal biotechnology o Value-Added Products and its potential as a modern “green gold rush.” The final chapter provides a an overview of advanced techniques such as genetic engineering of l g microalgae to increase lipid yield. This book provides a one-stop a benchmark reference on microalgal biotechnology, considering all aspects, e from microalgal screening to production of biofuels and other value- added products. K15038 ISBN: 978-1-4665-1529-1 Edited by 90000 Faizal Bux 9 781466 515291 K15038_Cover_mech.indd All Pages 4/22/13 1:28 PM Biotechnological Applications of Microalgae Biodiesel and Value-Added Products Biotechnological Applications of Microalgae Biodiesel and Value-Added Products Edited by Faizal Bux Boca Raton London New York CRC Press is an imprint of the Taylor & Francis Group, an informa business CRC Press Taylor & Francis Group 6000 Broken Sound Parkway NW, Suite 300 Boca Raton, FL 33487-2742 © 2013 by Taylor & Francis Group, LLC CRC Press is an imprint of Taylor & Francis Group, an Informa business No claim to original U.S. Government works Version Date: 20130409 International Standard Book Number-13: 978-1-4665-1530-7 (eBook - PDF) This book contains information obtained from authentic and highly regarded sources. Reasonable efforts have been made to publish reliable data and information, but the author and publisher cannot assume responsibility for the validity of all materials or the consequences of their use. The authors and publishers have attempted to trace the copyright holders of all material reproduced in this publication and apologize to copyright holders if permission to publish in this form has not been obtained. If any copyright material has not been acknowledged please write and let us know so we may rectify in any future reprint. Except as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced, transmitted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter invented, includ- ing photocopying, microfilming, and recording, or in any information storage or retrieval system, without written permission from the publishers. For permission to photocopy or use material electronically from this work, please access www.copyright.com (http://www.copyright.com/) or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that provides licenses and registration for a variety of users. For organizations that have been granted a photocopy license by the CCC, a separate system of payment has been arranged. Trademark Notice: Product or corporate names may be trademarks or registered trademarks, and are used only for identification and explanation without intent to infringe. Visit the Taylor & Francis Web site at http://www.taylorandfrancis.com and the CRC Press Web site at http://www.crcpress.com Contents Preface......................................................................................................................vii Acknowledgments .....................................................................................................ix About the Editor ........................................................................................................xi Contributors ...........................................................................................................xiii Chapter 1 Introduction ..........................................................................................1 Taurai Mutanda Chapter 2 The Biology of Microalgae ..................................................................7 Ranganathan Rajkumar and Zahira Yaakob Chapter 3 Strain Selection for Biodiesel Production ..........................................17 Subburamu Karthikeyan Chapter 4 Enumeration of Microalgal Cells .......................................................45 Taurai Mutanda and Faizal Bux Chapter 5 Microalgal Cultivation Reactor Systems ............................................51 Melinda J. Griffiths Chapter 6 Harvesting of Microalgal Biomass .....................................................77 Manjinder Singh, Rekha Shukla, and Keshav Das Chapter 7 Lipid Identification and Extraction Techniques .................................89 Desikan Ramesh Chapter 8 Synthesis of Biodiesel/Bio-Oil from Microalgae ...............................99 Bhaskar Singh, Yun Liu, and Yogesh C. Sharma v vi Contents Chapter 9 Analysis of Microalgal Biorefineries for Bioenergy from an Environmental and Economic Perspective Focus on Algal Biodiesel ...........................................................................................113 Susan T.L. Harrison, Christine Richardson, and Melinda J. Griffiths Chapter 10 Value-Added Products from Microalgae..........................................137 Terisha Naidoo, Nodumo Zulu, Dheepak Maharajh, and Rajesh Lalloo Chapter 11 Algae-Mediated Carbon Dioxide Sequestration for Climate Change Mitigation and Conversion to Value-Added Products ........161 Ajam Y. Shekh, Kannan Krishnamurthi, Raju R. Yadav, Sivanesan S. Devi, Tapan Chakrabarti, Sandeep N. Mudliar, Vikas S. Chauhan, Ravi Sarada, and Sanniyasi Elumalai Chapter 12 Phycoremediation by High-Rate Algal Ponds (HRAPs) ..................179 Ismail Rawat, Ramanathan Ranjith Kumar, and Faizal Bux Chapter 13 Microalgal Biotechnology: Today’s (Green) Gold Rush ..................201 Ravi V. Durvasula, Durvasula V. Subba Rao, and Vadrevu S. Rao Preface Over the decades, much of the literature has focused on the biological and ecological aspects of algae found in freshwater, marine, and brackish environ- ments. These organisms are also known to inhabit various other environments on Earth. More recently, there has been a substantial shift toward the concept of sustainable development and the “green economy” with emphasis on exploiting biological systems for the benefit of mankind. This underpins the fundamentals of the field of biotechnology, which has revolutionized various fields including agri- culture, food, pharmaceutical and medical sciences, environmental sciences, and industrial feedstock, thus positively impacting most spheres of human endeavor. Algae—but more specifically, microalgae—have been associated with problem- atic events such as algal blooms caused by eutrophication of aquatic environments, and in some cases the toxins produced have serious health impacts on the aquatic environment, plants, animals, and humans. However, these events are largely due to human activities resulting in the proliferation of nutrients in aquatic environ- ments. The significance of algae cannot be underestimated as they contribute approximately 40% of the oxygen in the atmosphere, are the original source of fossil fuels, and are the primary producers in the oceans. Therefore, there is poten- tial for exploitation of this invaluable biomass source that could lead to definite environmental and economic benefits for man. Therefore, the present book attempts to encompass the latest developments and recent research thrust and trends focusing specifically on the potential biotechno- logical application of microalgae. Strain selection, growth characteristics, large- scale culturing, and biomass harvesting have been discussed in detail and critically evaluated. With regard to the benefits of using algal technology, the major areas of progress have been in the fields of biodiesel production using microalgae, screening and production of high-value products from algae, and evaluating carbon dioxide sequestration from flue gas as a climate change mitigation strategy. The latter areas of research are clearly central to a sustainable development approach that is cur- rently attracting global attention. Compared to conventional crops, biodiesel from microalgae has shown superior potential in terms of yields and using wastewater as a substrate. However, critical evaluation of the technology has signaled some limitations with regard to large-scale biomass processing, specifically with regard to harvesting and oil extraction. Globally, much attention is currently focused on overcoming these hurdles to improve the techno-economics and make algal bio- diesel a fuel for tomorrow. Findings have shown that a biorefinery approach will certainly aid in improving the economics and large-scale application of the tech- nology. Producing high-value products (health care products, pigments, etc.) spe- cifically using microalgae is a large income-generating industry in many parts of the world, with potential for substantial growth. In addition, algal technology can be used as a cheaper option for the treatment of wastewater streams. vii viii Preface Therefore, this book will serve as an excellent reference for researchers and prac- titioners, providing a comprehensive outline of the most recent developments and advances in the field of biotechnological applications of microalgae. I would like to thank all the authors for their contributions to this book. Professor Faizal Bux Institute for Water and Wastewater Technology Durban University of Technology

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"The book gives an in-depth analysis of microalgal biology, ecology, biotechnology and biofuel production capacity as well as a thorough discussion on the value added products that can be generated from diverse microalgae. It summarizes the state of the art in microalgal biotechnology research, from
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