Saccharomyces cerevisiae

101 Pages·2016·6.33 MB·English

BIODIVERSITY OF ALCOHOL PRODUCING
YEAST- Saccharomyces cerevisiae
By
PISE SATISH AMBADAS
B. Sc. (Ag.)

THESIS SUBMITTED TO THE
ACHARYA N.G.RANGA AGRICULTURAL UNIVERSITY
IN PARTIAL FULFILMENT OF THE REQUIREMENTS
FOR THE AWARD OF THE DEGREE OF

MASTER OF SCIENCE IN AGRICULTURE
(AGRICULTURAL MICROBIOLOGY AND BIOENERGY)

CHAIRPERSON: Dr. S. SUMATHI

DEPARTMENT OF AGRICULTURAL MICROBIOLOGY AND
BIOENERGY
COLLEGE OF AGRICULTURE
RAJENDRANAGAR, HYDERABAD-500 030
ACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY
2011
BIODIVERSITY OF ALCOHOL
PRODUCING YEAST- Saccharomyces
cerevisiae

PPIISSEE SSAATIISSHH AAMMBBAADDAASS
B.Sc. (Ag.)

MASTER OF SCIENCE IN AGRICULTURE
(AGRICULTURAL MICROBIOLOGY)

2011
CERTIFICATE
Mr. PISE SATISH AMBADAS has satisfactorily prosecuted the course of
research and that the thesis entitled “BIODIVERSITY OF ALCOHOL PRODUCING
YEAST- Saccharomyces cerevisiae” submitted is the result of original research work and
is of sufficiently high standard to warrant its presentation to the examination. I also certify
that neither the thesis nor its part thereof has been previously submitted by him for a degree
of any University.

Chairperson
Date
CERTIFICATE
This is to certify that the thesis entitled “BIODIVERSITY OF ALCOHOL
PRODUCING YEAST- Saccharomyces cerevisiae” submitted in partial fulfilment of the
requirements for the degree of ‘Master of Science in Agriculture’ of the Acharya
N.G.Ranga Agricultural University, Hyderabad is a record of the bonafide original research
work carried out by Mr. PISE SATISH AMBADAS under our guidance and supervision.
No part of the thesis has been submitted by the student for any other degree or
diploma. The published part and all the assistance received during the course of
investigations have been duly acknowledged by the author of the thesis.
Thesis approved by the Student’s Advisory Committee
Chairperson : Dr. S. Sumathi
Professor and Univ. Head,
Department of Biochemistry,
College of Agriculture, ANGRAU,
Rajendranagar, Hyderabad-500 030. _____________________
Member : Dr. R. Subhash Reddy
Professor and Univ. Head,
Department of Agril. Microbiology
and Bioenergy,
College of Agriculture, ANGRAU,
Rajendranagar, Hyderabad-500 030. _____________________
Member : Dr.S. Sokka Reddy,
Professor and Head,
Department of Agril. Biotechnology,
Rajendranagar, Hyderabad-500 030. _____________________

Date of final viva-voce:
DECLARATION
I, PISE SATISH AMBADAS, hereby declare that the thesis entitled
“BIODIVERSITY OF ALCOHOL PRODUCING YEAST- Saccharomyces cerevisiae”
submitted to the Acharya N.G. Ranga Agricultural University for the degree of Master
of Science in Agriculture is a result of original research work done by me. I also declare
that no material contained in the thesis has been published earlier in any manner.

Place: Hyderabad (PISE SATISH AMBADAS)
I. D. No. - RAM/09-84
Date
LIST OF CONTENTS
Page No.
Title
Chapter No.
I
INTRODUCTION
II REVIEW OF LITERATURE
III MATERIALS AND METHODS
IV RESULTS AND DISCUSSION
V SUMMARY AND CONCLUSIONS
LITERATURE CITED
APPENDICES
ACKNOWLEDGEMENT
With immense pleasure, I wish to express my heartfelt respect and affectionate gratitude to my
beloved father Ambadas pise and mother Sheela pise whose everlasting love, unfading faith, incessant
inspiration, moral and financial support and blessings kept me enthusiastic throughout my life and
molded me to the present position, and my dear sister Anita Gadekar whose constant encouragement
brings out the best in every one of my endeavors and without which this work could not be completed.
I humbly take this opportunity to place my profound debt of gratitude on record to my esteemed
major advisor and Chairperson of the Advisory Committee, Dr. S. Sumathi, Professor and Univ. Head
Department of Biochemistry, College of Agriculture, Rajendranagar, Hyderabad for her valuable
suggestions, encouragement, affection, personal guidance, keen interest, immeasurable help and
constructive criticism given throughout my work and making it possible to bring out this thesis. It was
an honor and privilege for me to be associated with her who stood by me with boundless patience in
many difficult situations during my M. Sc. Degree Programme.
I am equally and deeply indebted to Dr. R. Subhash Reddy, Professor and Univ. Head,
Department of Agricultural Microbiology and Bioenergy, College of Agriculture, Rajendranagar,
Hyderabad and co-chairman of the Advisory Committee for giving me the privilege for full utilization of
all laboratory facilities. His encouragement, valuable guidance and technical suggestions throughout the
period of investigation led to the perfect conduct of the experimental work and presentation of thesis.
I owe my effusive thanks to Dr. S. Sokka Reddy Professor and Univ. Head, Department of
Agricultural Biotechnology, College of Agriculture, Rajendranagar, Hyderabad and Member of Advisory
Committee for his encouragement, co-operation and valuable suggestions during the course of my study.
I express my sincere gratitude to Dr.Reshma guest faculty, Agricultural Microbiology and
Bioenergy, college of Agriculture, Rajendranagar, Hyderabad for her valuable counsel, note-worthy
guidance and cordial co-operation during the course of investigation.
Words fail to express my deepest sense of gratitude to Reshma Bhadange, Krishna, Surendra
and Niranjan for their unabated help, co-operation, affection, and encouragement and even to the extent
of keeping aside their personal work and giving their valuable time so that I could finish my work at the
earliest possible time.
I am really indebted to my dear friends Mahesh, Ganga prasad, Sachin, and Alok, for their
great support, help and encouragement. I also fondly thank my senior friends Jaya, Manoj and Navin for
providing valuable guidance and to my juniors Harish, Tirupati, Ganesh, Rahul and Manoj for rendering
their unending co-operation and help during the course of my research.
I wish to express my heartfelt thanks to my classmates Shanti, Madhavi, Supraja and Revati
for their lovely friendship, help and care and for making the two year study very much enjoyable and
memorable.
I acknowledge ICAR for providing a seat in this esteemed university. I am also thankful to the
authorities of Acharya N.G. Ranga Agricultural University for providing excellent milieu and facilities
in completion of my post graduation.
I take this opportunity to convey my wholehearted thanks to all my family members, relatives
and well wishers, requesting their forgiveness for not mentioning them here by name.
Above all, I am grateful to ‘Almighty God’ for giving me enough strength and fortitude to meet
various challenges.
Needless to say omissions and errors are mine.

Hyderabad (SATISH A PISE)
June, 2011
LIST OF SYMBOLS AND ABBREVIATIONS

% : Per cent
m : Micro meter
< : Less than
> : Greater than
µg ml-1 : Micro gram per milli litre
µg : Microgram (s)
µl : Micro litre
~ : Similar to
& : And
CD : Critical difference
CFU : Colony forming units
CRD : Completely randomized design
et al. : And others
Fig. : Figure
g l-1 : Gram per litre
g : Gram
h : Hour (s)
i.e. : That is
IMTECH : Institute of Microbial Technology
kg : Kilogram
KMS : Potassium metabisulphate
l : Litre (s)
M : Molar
mg : Milligram (s)
min : Minute (s)
ml : Millilitres (s)
l yr-1 : Liters per year
mm : Millimetre (s)
MTCC : Microbial Type Culture Collection
NaOH : Sodium hydroxide
ng µl-1 : Nano gram per micro litre
nm : Nanometer
no. : Number
Sr. No. : Serial number
oC : Degree celsius
OD : Optical density
pH : Negative logarithm of hydrogen ion activity
ppm : Parts per million
rpm : Rotation per minute
v/v : Volume by volume
viz. : Namely
w/v : Weight/volume
wt. : Weight